Review
Biotechnology & Applied Microbiology
Eugenia Carraro, Lucia Rossi, Edoardo Maghin, Marcella Canton, Martina Piccoli
Summary: This review discusses the importance of generating human skeletal muscle three-dimensional models through tissue engineering approaches, including the study of the most severe myopathies and the comparison of different in vitro models, as well as their effectiveness in simulating disease mechanisms and investigating therapeutic effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Haneul Kim, Gon Sup Kim, Sang-Hwan Hyun, Eunhye Kim
Summary: Neuromuscular diseases involve injury or dysfunction of neuromuscular tissue components. In vitro models have been developed to mimic the complex physical and biochemical microenvironments of human neuromuscular tissues, aiding the study and potential therapies for these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Review
Cell Biology
Paolo Raffa, Maria Easler, Anna Urciuolo
Summary: Skeletal muscle is reliant on the clear three-dimensional organization of myofibers and communication between myofibers and the nervous system for development and function. Various pathologies affecting muscle function are linked to alterations in the interaction between motor neurons and myofibers. Recent research indicates that three-dimensionality and integration of multiple cell types are essential for unveiling pathophysiologically relevant phenotypes in skeletal muscle modeling.
NEURAL REGENERATION RESEARCH
(2022)
Review
Biology
Camila Vesga-Castro, Javier Aldazabal, Ainara Vallejo-Illarramendi, Jacobo Paredes
Summary: In recent years, there has been a growing interest in evaluating the contractile force (CF) of engineered muscle tissues, but there are currently no standards available for selecting the most suitable experimental platform, measuring system, culture protocol, or stimulation patterns. Cantilever deflection, post deflection, and force transducers are commonly used configurations for CF assessment, and future studies are recommended to report detailed information on construct size, contractile area, maturity level, sarcomere length, and the tetanus-to-twitch ratio.
Article
Engineering, Biomedical
Jeong Sik Kong, Xuan Huang, Yeong-Jin Choi, Hee-Gyeong Yi, Junsu Kang, Sejin Kim, Jongmin Kim, Hyungseok Lee, Yeri Alice Rim, Ji Hyeon Ju, Wan Kyun Chung, Clifford J. Woolf, Jinah Jang, Dong-Woo Cho
Summary: 3D cell printing technology is widely studied for medical applications, with a particular focus on neuromuscular tissue. The use of CNSdECM bioink for 3D neuromuscular tissue fabrication shows promising results in supporting motor neuron activity and axon growth. This bioink holds great potential for producing tissue-engineered motor systems using 3D cell printing.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Erin Rousseau, Ritu Raman, Tigist Tamir, Angel Bu, Shriya Srinivasan, Naomi Lynch, Robert Langer, Forest M. White, Michael J. Cima
Summary: Damage to skeletal muscle tissue can have a severe impact on health and mobility. The study shows that targeted activation of implanted muscle grafts through non-invasive light stimulation restores motor function to levels similar to healthy mice. Analysis also reveals that repeated muscle contraction alters signaling pathways involved in muscle contractility, injury adaptation, neurite growth, neuromuscular synapse formation, angiogenesis, and cytoskeletal remodeling.
Article
Engineering, Biomedical
Xiomara Fernandez-Garibay, Maria A. Ortega, Estefania Cerro-Herreros, Jordi Comelles, Elena Martinez, Ruben Artero, Juan M. Fernandez-Costa, Javier Ramon-Azcon
Summary: The article describes the development of the first in vitro 3D model of DM1 human skeletal muscle, which shows highly aligned myotubes from both healthy and DM1 cells. The 3D micropatterning significantly improved DM1 cell differentiation into multinucleated myotubes, suitable for drug testing. AntagomiR-23b treatment in bioengineered DM1 skeletal muscle microtissues demonstrates significant therapeutic effects in rescuing molecular DM1 hallmarks and structural phenotypes.
Review
Biotechnology & Applied Microbiology
Natalie G. Kozan, Mrunmayi Joshi, Sydnee T. Sicherer, Jonathan M. Grasman
Summary: This article reviews the methods and materials used for fabricating porous biomaterial scaffolds for skeletal muscle regeneration. It discusses the current state of skeletal muscle tissue engineering and provides guidelines for scaffold fabrication based on functional requirements.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
E. Fornetti, F. De Paolis, C. Fuoco, S. Bernardini, S. M. Giannitelli, A. Rainer, D. Seliktar, F. Magdinier, J. Baldi, R. Biagini, S. Cannata, S. Testa, C. Gargioli
Summary: Three-dimensional (3D) bioprinting has emerged as an optimal tool for tissue engineering. The extrusion-based approach is the most suitable for skeletal muscle tissue engineering due to its ability to produce parallel printing fibers that mimic native tissue architecture. The development of a novel extrusion-based 3D bioprinting system using PEG-Fibrinogen has shown promising results for muscle tissue regeneration, both in vitro and in vivo.
Article
Chemistry, Multidisciplinary
Sadegh Davoudi, Bin Xu, Erik Jacques, Jose L. Cadavid, Matthew McFee, Chih-Ying Chin, Amir Meysami, Majid Ebrahimi, Mohsen A. Bakooshli, Kayee Tung, Henry Ahn, Howard J. Ginsberg, Alison P. McGuigan, Penney M. Gilbert
Summary: Stem cell-mediated endogenous repair is often evaluated through animal studies, but this study describes an in vitro assay that mimics important early stages of in vivo skeletal muscle repair. By integrating a semi-automated image analysis pipeline, high-content data analysis of muscle fiber content and morphology is enabled. The study found that the spatiotemporal dynamics of in vitro repair closely matched those observed in vivo only when both stem cells and injury were present. The engineered tissue's imaging geometry allowed for exploration of cellular mechanisms driving the muscle stem cell response to the regenerative template.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Cell Biology
X. Wei, C. Nicoletti, P. L. Puri
Summary: This article discusses the emerging contribution of FAPs to skeletal muscle responses to motor neuron injuries and systemic physiological or pathological metabolic perturbations, providing an initial blueprint of discrete sub-clusters of FAPs activated by specific perturbations and their role in muscle adaptation to these conditions.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Elana Molotsky, Yuhong Liu, Andrew P. Lieberman, Diane E. Merry
Summary: This study examines the relationship between neuromuscular junction (NMJ) pathology and vulnerability of fast-twitch motor units in spinal and bulbar muscular atrophy (SBMA) mouse models. The research reveals significantly increased NMJ and myofiber pathology in fast-twitch motor units, along with metabolic dysregulation and myofiber atrophy.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Nicolas Rose, Berenice Estrada Chavez, Surabhi Sonam, Thao Nguyen, Gianluca Grenci, Anne Bigot, Antoine Muchir, Benoit Ladoux, Bruno Cadot, Fabien Le Grand, Lea Trichet
Summary: We have developed a miniaturized 3D myotube culture chip that can monitor contraction at the single cell level. This technology requires significantly fewer starting materials than current systems and is crucial for evaluating the outcomes of therapeutic procedures for neuromuscular disorders.
Article
Biotechnology & Applied Microbiology
Lisanne Terrie, Margherita Burattini, Sandra Van Vlierberghe, Lorenzo Fassina, Lieven Thorrez
Summary: Skeletal muscle tissue engineering aims to generate 3D engineered constructs that mimic the structure and function of native muscle. Pulsed electromagnetic field stimulation enhances myotube formation in both 2D and 3D cultures, increasing myotube diameter, fusion index, and myosin heavy chain expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stanley M. H. Chan, Claudia Cerni, Samantha Passey, Huei Jiunn Seow, Ivan Bernardo, Chris van der Poel, Aleksandar Dobric, Kurt Brassington, Stavros Selemidis, Steven Bozinovski, Ross Vlahos
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2020)
Article
Cell Biology
Alessandro Bertero, Paul A. Fields, Alec S. T. Smith, Andrea Leonard, Kevin Beussman, Nathan J. Sniadecki, Deok-Ho Kim, Hung-Fat Tse, Lil Pabon, Jay Shendure, William S. Noble, Charles E. Murry
JOURNAL OF CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Jason W. Miklas, Elisa Clark, Shiri Levy, Damien Detraux, Andrea Leonard, Kevin Beussman, Megan R. Showalter, Alec T. Smith, Peter Hofsteen, Xiulan Yang, Jesse Macadangdang, Tuula Manninen, Daniel Raftery, Anup Madan, Anu Suomalainen, Deok-Ho Kim, Charles E. Murry, Oliver Fiehn, Nathan J. Sniadecki, Yuliang Wang, Hannele Ruohola-Baker
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
M. L. Hepworth, S. L. Passey, H. J. Seow, R. Vlahos
SCIENTIFIC REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Sarah A. Najjar, Alexander S. T. Smith, Christopher J. Long, Christopher W. McAleer, Yunqing Cai, Balaji Srinivasan, Candace Martin, Herman H. Vandenburgh, James J. Hickman
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Chemistry, Multidisciplinary
Alec S. T. Smith, Eunpyo Choi, Kevin Gray, Jesse Macadangdang, Eun Hyun Ahn, Elisa C. Clark, Michael A. Laflamme, Joseph C. Wu, Charles E. Murry, Leslie Tung, Deok-Ho Kim
Article
Chemistry, Multidisciplinary
Jong Seob Choi, Alec S. T. Smith, Nisa P. Williams, Tatsuya Matsubara, Minji Choi, Joon-Wan Kim, Hyung Jin Kim, Seungkeun Choi, Deok-Ho Kim
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Engineering, Biomedical
Nisa P. Williams, Marcus Rhodehamel, Calysta Yan, Alec S. T. Smith, Alex Jiao, Charles E. Murry, Marta Scatena, Deok-Ho Kim
Article
Engineering, Biomedical
Changho Chun, Alec S. T. Smith, Hyejin Kim, Dana S. Kamenz, Jung Hyun Lee, Jong Bum Lee, David L. Mack, Mark Bothwell, Claire D. Clelland, Deok-Ho Kim
Summary: This study investigated the impact of astrocyte-derived extracellular vesicles (EVs) on human neurons cultured in vitro, demonstrating that these EVs can enhance the electrophysiological function and anti-apoptotic behavior of neurons. The cargo proteins found in the EVs include various neuroprotective proteins and proteins that positively regulate neuronal excitability and synaptic development, suggesting the potential for EV-based therapeutics in treating neuropathies.
Article
Engineering, Biomedical
Jonathan H. Tsui, Andrea Leonard, Nathan D. Camp, Joseph T. Long, Zeid Y. Nawas, Rakchanok Chavanachat, Alec S. T. Smith, Jong Seob Choi, Zhipeng Dong, Eun Hyun Ahn, Alejandro Wolf-Yadlin, Charles E. Murry, Nathan J. Sniadecki, Deok-Ho Kim
Summary: Hybrid hydrogels composed of decellularized porcine myocardial extracellular matrix (dECM) and reduced graphene oxide (rGO) were developed to improve the functionality of cardiomyocytes. These hydrogels can also be used as bioinks to print cardiac tissues in a high-throughput manner. Modulating the composition and properties of the materials can enhance the electrophysiological function of cardiac tissues.
Article
Cell Biology
Alec S. T. Smith, Changho Chun, Jennifer Hesson, Julie Mathieu, Paul N. Valdmanis, David L. Mack, Byung-Ok Choi, Deok-Ho Kim, Mark Bothwell
Summary: Gene editing technologies can enhance modeling of inheritable neurodegenerative diseases, but careful comparison of multiple cell lines is needed to ensure consistency and accuracy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Materials Science, Biomaterials
Alec S. T. Smith, Jong Hyun Kim, Changho Chun, Ava Gharai, Hyo Won Moon, Eun Young Kim, Soo Hyun Nam, Nina Ha, Ju Young Song, Ki Wha Chung, Hyun Myung Doo, Jennifer Hesson, Julie Mathieu, Mark Bothwell, Byung-Ok Choi, Deok-Ho Kim
Summary: This study used human induced pluripotent stem cell models to investigate CMT2D disease, finding significant neuronal activity defects in cells with GARS1 mutations, and treatment with histone deacetylase 6 inhibitor CKD504 can improve these defects.
Article
Multidisciplinary Sciences
Dylan Shea, Elizabeth Colasurdo, Alec Smith, Courtnie Paschall, Suman Jayadev, C. Dirk Keene, Douglas Galasko, Andrew Ko, Ge Li, Elaine Peskind, Valerie Daggett
Summary: The formation of toxic Amyloid beta-peptide oligomers is an early event in Alzheimer's Disease. A designed alpha-sheet peptide can inhibit the deleterious effects on neuronal signaling and serve as a capture agent in a soluble oligomer binding assay. This method can detect toxic oligomers in Alzheimer's Disease and discriminate them from other forms of dementia.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Alec S. T. Smith, Shawn M. Luttrell, Jean-Baptiste Dupont, Kevin Gray, Daniel Lih, Jacob W. Fleming, Nathan J. Cunningham, Sofia Jepson, Jennifer Hesson, Julie Mathieu, Lisa Maves, Bonnie J. Berry, Elliot C. Fisher, Nathan J. Sniadecki, Nicholas A. Geisse, David L. Mack
Summary: Engineered muscle tissues combined with a magnetic sensing system allow non-invasive longitudinal analysis of developing contraction kinetics, improving research efficiency and drug screening capabilities. The platform enables identification and classification of healthy and diseased muscle cells, and has significant implications for the translation of novel therapies.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Green & Sustainable Science & Technology
Greg Potter, Alec S. T. Smith, Nguyen T. K. Vo, Jeanot Muster, Wendy Weston, Alessandro Bertero, Lisa Maves, David L. Mack, Alain Rostain