A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Toxoplasma Controls Host Cyclin E Expression through the Use of a Novel MYR1-Dependent Effector Protein, HCE1

(2019) Michael W. Panas et al.   mBio

The Toxoplasma effector TEEGR promotes parasite persistence by modulating NF-κB signalling via EZH2

(2019) Laurence Braun et al.   Nature Microbiology

Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Orchestrate Chronic Infection and GRA12 Underpins Resistance to Host Gamma Interferon

(2019) Barbara A. Fox et al.   mBio

CRISPR Screen Reveals that EHEC’s T3SS and Shiga Toxin Rely on Shared Host Factors for Infection

(2018) Alline R. Pacheco et al.   mBio

MYR1-Dependent Effectors Are the Major Drivers of a Host Cell’s Early Response to Toxoplasma , Including Counteracting MYR1-Independent Effects

(2018) Adit Naor et al.   mBio

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

(2018) Tianmin Wang et al.   Nature Communications

Identification of a novel protein complex essential for effector translocation across the parasitophorous vacuole membrane of Toxoplasma gondii

(2018) Nicole D. Marino et al.   PLoS Pathogens

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

(2016) Saima M. Sidik et al.   CELL

CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity

(2016) Carlos J. Blondel et al.   Cell Host & Microbe

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B

(2016) Liang Tao et al.   NATURE

The Toxoplasma gondii Rhoptry Kinome Is Essential for Chronic Infection

(2016) Barbara A. Fox et al.   mBio

A Novel Secreted Protein, MYR1, Is Central to Toxoplasma ’s Manipulation of Host Cells

(2016) Magdalena Franco et al.   mBio

In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis

(2016) Santhosh M. Nadipuram et al.   mBio

The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole

(2015) Daniel A. Gold et al.   Cell Host & Microbe

A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus

(2015) Qingpeng Ren et al.   Scientific Reports

The Toxoplasma Pseudokinase ROP5 Forms Complexes with ROP18 and ROP17 Kinases that Synergize to Control Acute Virulence in Mice

(2014) Ronald D. Etheridge et al.   Cell Host & Microbe

GRA25 Is a Novel Virulence Factor of Toxoplasma gondii and Influences the Host Immune Response

(2014) A. J. Shastri et al.   INFECTION AND IMMUNITY

Toxoplasma gondiiVirulence Factor ROP18 Inhibits the Host NF-κB Pathway by Promoting p65 Degradation

(2014) Jian Du et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

E-CRISP: fast CRISPR target site identification

(2014) Florian Heigwer et al.   NATURE METHODS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Host Cell Subversion by Toxoplasma GRA16, an Exported Dense Granule Protein that Targets the Host Cell Nucleus and Alters Gene Expression

(2013) Alexandre Bougdour et al.   Cell Host & Microbe

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

The Phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii Reveal Unusual Adaptations Within and Beyond the Parasites' Boundaries

(2011) Moritz Treeck et al.   Cell Host & Microbe

Type II Toxoplasma gondii KU80 Knockout Strains Enable Functional Analysis of Genes Required for Cyst Development and Latent Infection

(2011) Barbara A. Fox et al.   EUKARYOTIC CELL

ATF6β is a host cellular target of theToxoplasma gondiivirulence factor ROP18

(2011) Masahiro Yamamoto et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Strain-specific activation of the NF-κB pathway by GRA15, a novelToxoplasma gondiidense granule protein

(2011) Emily E. Rosowski et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Phosphorylation of Immunity-Related GTPases by a Toxoplasma gondii-Secreted Kinase Promotes Macrophage Survival and Virulence

(2010) Sarah J. Fentress et al.   Cell Host & Microbe

Phosphorylation of Mouse Immunity-Related GTPase (IRG) Resistance Proteins Is an Evasion Strategy for Virulent Toxoplasma gondii

(2010) Tobias Steinfeldt et al.   PLOS BIOLOGY

Toxoplasmosis snapshots: Global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis

(2009) Georgios Pappas et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

GRA12, a Toxoplasma dense granule protein associated with the intravacuolar membranous nanotubular network

(2008) Adeline Michelin et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY