Recent advances in the improvement of enzyme thermostability by structure modification

How to calculate thermostability of enzymes using a simple approach

(2018) Abdul A. N. Saqib et al.   BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION

Computer-assisted engineering of hyperstable fibroblast growth factor 2

(2018) Pavel Dvorak et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast

(2018) Fei Ge et al.   BIOTECHNOLOGY LETTERS

Enzymatic asymmetric synthesis of chiral amino acids

(2018) Ya-Ping Xue et al.   CHEMICAL SOCIETY REVIEWS

Engineering the residues on “A” surface and C-terminal region to improve thermostability of nitrilase

(2018) Zhe Xu et al.   ENZYME AND MICROBIAL TECHNOLOGY

Structural and functional insights into thermostable and organic solvent stable variant Pro247-Ser of Bacillus lipase

(2018) Nisha Chopra et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Rapid Sampling of Hydrogen Bond Networks for Computational Protein Design

(2018) Jack B. Maguire et al.   Journal of Chemical Theory and Computation

Structural investigation of the enantioselectivity and thermostability mechanisms of esterase RhEst1

(2018) Qi Chen et al.   JOURNAL OF MOLECULAR GRAPHICS & MODELLING

Enhancing Protein Stability with Genetically Encoded Noncanonical Amino Acids

(2018) Jack C. Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multifactorial level of extremostability of proteins: can they be exploited for protein engineering?

(2017) Debamitra Chakravorty et al.   EXTREMOPHILES

Crystal structure of a lipase from Streptomyces sp. strain W007 - implications for thermostability and regiospecificity

(2017) Zexin Zhao et al.   FEBS Journal

Directed Evolution of Recombinant C-Terminal Truncated Staphylococcus epidermidis Lipase AT2 for the Enhancement of Thermostability

(2017) Jiivittha Veno et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Computational Design of a Photocontrolled Cytosine Deaminase

(2017) Kristin M. Blacklock et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

FireProt: web server for automated design of thermostable proteins

(2017) Milos Musil et al.   NUCLEIC ACIDS RESEARCH

Enzyme stabilization via computationally guided protein stapling

(2017) Eric J. Moore et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Peptide backbone circularization enhances antifreeze protein thermostability

(2017) Corey A. Stevens et al.   PROTEIN SCIENCE

Tricolor R/G/B Laser Diode Based Eye-Safe White Lighting Communication Beyond 8 Gbit/s

(2017) Tsai-Chen Wu et al.   Scientific Reports

Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase

(2016) Junxian Zheng et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering the thermostability of β-glucuronidase from Penicillium purpurogenum Li-3 by loop transplant

(2016) Xudong Feng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Construction of Thermophilic Xylanase and Its Structural Analysis

(2016) Masahiro Watanabe et al.   BIOCHEMISTRY

Comparative thermal inactivation analysis ofAspergillus oryzaeandThiellavia terrestriscutinase: Role of glycosylation

(2016) Abhijit N. Shirke et al.   BIOTECHNOLOGY AND BIOENGINEERING

Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

(2016) Khawar Sohail Siddiqui   CRITICAL REVIEWS IN BIOTECHNOLOGY

Modulation of the thermostability and substrate specificity of Candida rugosa lipase1 by altering the acyl-binding residue Gly414 at the α-helix-connecting bend

(2016) Xiaofei Zhang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Dramatically Improved Performance of an Esterase for Cilastatin Synthesis by Cap Domain Engineering

(2016) Zheng-Jiao Luan et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Enhancing the Thermostability of β-Glucuronidase by Rationally Redesigning the Catalytic Domain Based on Sequence Alignment Strategy

(2016) Xudong Feng et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Thermostability of Enzymes from Molecular Dynamics Simulations

(2016) Tim Zeiske et al.   Journal of Chemical Theory and Computation

Contribution of main chain and side chain atoms and their locations to the stability of thermophilic proteins

(2016) Dharma Rao Tompa et al.   JOURNAL OF MOLECULAR GRAPHICS & MODELLING

An extended loop in CE7 carbohydrate esterase family is dispensable for oligomerization but required for activity and thermostability

(2016) Mrityunjay K. Singh et al.   JOURNAL OF STRUCTURAL BIOLOGY

Experimental and Computational Analysis of Protein Stabilization by Gly-to-d-Ala Substitution: A Convolution of Native State and Unfolded State Effects

(2016) Junjie Zou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural and dynamic evolution of the amphipathic N-terminus diversifies enzyme thermostability in the glycoside hydrolase family 12

(2016) Xukai Jiang et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Enhancing protein stability with extended disulfide bonds

(2016) Tao Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolutionary drivers of thermoadaptation in enzyme catalysis

(2016) Vy Nguyen et al.   SCIENCE

SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase

(2016) Meng Si et al.   PLoS One

Engineering de novo disulfide bond in bacterial α-type carbonic anhydrase for thermostable carbon sequestration

(2016) Byung Hoon Jo et al.   Scientific Reports

Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution

(2015) Amol V. Shivange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The role of proline substitutions within flexible regions on thermostability of luciferase

(2015) Haoran Yu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Recent Advances in Applied Biocatalysis and Biotechnology

(2015) Rodrigo O.M.A. de Souza et al.   BIOTECHNOLOGY ADVANCES

Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta

(2015) Matthew J. O’Meara et al.   Journal of Chemical Theory and Computation

FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants

(2015) David Bednar et al.   PLoS Computational Biology

Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis

(2015) Prakash Chandra Rathi et al.   PLoS One

Thermostability enhancement of an endo-1,4-β-galactanase from Talaromyces stipitatus by site-directed mutagenesis

(2014) Dorte M. Larsen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability

(2014) Jian-Ping Wu et al.   BIOTECHNOLOGY LETTERS

Enhanced Enzyme Kinetic Stability by Increasing Rigidity within the Active Site

(2014) Yuan Xie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Protein Thermal Stability Enhancement by Designing Salt Bridges: A Combined Computational and Experimental Study

(2014) Chi-Wen Lee et al.   PLoS One

Computationally designed libraries for rapid enzyme stabilization

(2014) H. J. Wijma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Systematic Optimization of Interface Interactions Increases the Thermostability of a Multimeric Enzyme

(2013) Andreas Bosshart et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel

(2013) Tana Koudelakova et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

(2013) Adi Dror et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Deletion of a dynamic surface loop improves stability and changes kinetic behavior of phosphatidylinositol-synthesizingStreptomycesphospholipase D

(2013) Jasmina Damnjanović et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stabilizing biocatalysts

(2013) Andreas S. Bommarius et al.   CHEMICAL SOCIETY REVIEWS

Structure- and sequence-analysis inspired engineering of proteins for enhanced thermostability

(2013) Hein J Wijma et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Protein disulfide engineering

(2013) Alan A. Dombkowski et al.   FEBS LETTERS

Improved activity and thermostability of Bacillus pumilus lipase by directed evolution

(2013) Nagihan Akbulut et al.   JOURNAL OF BIOTECHNOLOGY

Hydrophobic environment is a key factor for the stability of thermophilic proteins

(2013) M. Michael Gromiha et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Automated selection of stabilizing mutations in designed and natural proteins

(2012) B. Borgo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality

(2011) Yves Dehouck et al.   BMC BIOINFORMATICS

Contribution of inter-subunit interactions to the thermostability of Pyrococcus furiosus citrate synthase

(2011) Vicky Moore et al.   EXTREMOPHILES

Computational design and selections for an engineered, thermostable terpene synthase

(2011) Juan E. Diaz et al.   PROTEIN SCIENCE

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A role for flexible loops in enzyme catalysis

(2010) M Merced Malabanan et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Interrelationship of Steric Stabilization and Self-Crowding of a Glycosylated Protein

(2009) R. Høiberg-Nielsen et al.   BIOPHYSICAL JOURNAL

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design

(2009) Oliver Spadiut et al.   FEBS Journal

Structures and Functional Implications of an AMP-Binding Cystathionine β-Synthase Domain Protein from a Hyperthermophilic Archaeon

(2008) Neil P. King et al.   JOURNAL OF MOLECULAR BIOLOGY

Effect of glycosylation on protein folding: A close look at thermodynamic stabilization

(2008) D. Shental-Bechor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thermostability of Salt Bridges versus Hydrophobic Interactions in Proteins Probed by Statistical Potentials

(2007) Benjamin Folch et al.   Journal of Chemical Information and Modeling