Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

Quantifying the Entropy of Binding for Water Molecules in Protein Cavities by Computing Correlations

(2015) David J. Huggins   BIOPHYSICAL JOURNAL

Enhanced functionality and stabilization of a cold active laccase using nanotechnology based activation-immobilization

(2015) Arka Mukhopadhyay et al.   BIORESOURCE TECHNOLOGY

A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity

(2015) Haluk Ertan et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Destructuring plant biomass: Focus on fungal and extremophilic cell wall hydrolases

(2015) Gea Guerriero et al.   PLANT SCIENCE

Structure-based rational design and introduction of arginines on the surface of an alkaline α-amylase from Alkalimonas amylolytica for improved thermostability

(2014) Zhuangmei Deng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improvement of the stability and activity of the BPO-A1 haloperoxidase from Streptomyces aureofaciens by directed evolution

(2014) Ryosuke Yamada et al.   JOURNAL OF BIOTECHNOLOGY

Thermostable Artificial Enzyme Isolated by In Vitro Selection

(2014) Aleardo Morelli et al.   PLoS One

Entropy-Enthalpy Compensation: Role and Ramifications in Biomolecular Ligand Recognition and Design

(2013) John D. Chodera et al.   Annual Review of Biophysics

Psychrophiles

(2013) Khawar S. Siddiqui et al.   Annual Review of Earth and Planetary Sciences

Structural and Energetic Basis of Protein Kinetic Destabilization in Human Phosphoglycerate Kinase 1 Deficiency

(2013) Angel L. Pey et al.   BIOCHEMISTRY

Engineering proteins for thermostability through rigidifying flexible sites

(2013) Haoran Yu et al.   BIOTECHNOLOGY ADVANCES

Deletion of a dynamic surface loop improves stability and changes kinetic behavior of phosphatidylinositol-synthesizingStreptomycesphospholipase D

(2013) Jasmina Damnjanović et al.   BIOTECHNOLOGY AND BIOENGINEERING

Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics

(2013) Tomasz Florczak et al.   ENZYME AND MICROBIAL TECHNOLOGY

Temperature and the catalytic activity of enzymes: A fresh understanding

(2013) Roy M. Daniel et al.   FEBS LETTERS

Substrate preference of a Geobacillus maltogenic amylase: A kinetic and thermodynamic analysis

(2013) Samira Nasrollahi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

A multi-factors rational design strategy for enhancing the thermostability of Escherichia coli AppA phytase

(2013) Baojin Fei et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Thermal stabilization of psychrophilic enzymes: A case study of the cold-active hormone-sensitive lipase from Psychrobacter sp. TA144

(2012) Concetta De Santi et al.   BIOTECHNOLOGY PROGRESS

Improving thermostability of phosphatidylinositol-synthesizing Streptomyces phospholipase D

(2012) J. Damnjanovic et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The Protein-Folding Problem, 50 Years On

(2012) K. A. Dill et al.   SCIENCE

Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop

(2011) Ya-Shan Cheng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Stepwise Adaptations to Low Temperature as Revealed by Multiple Mutants of Psychrophilic α-Amylase from Antarctic Bacterium

(2011) Alexandre Cipolla et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PHOENIX: A Scoring Function for Affinity Prediction Derived Using High-Resolution Crystal Structures and Calorimetry Measurements

(2011) Yat T. Tang et al.   Journal of Chemical Information and Modeling

Kinetic consequences of native state optimization of surface-exposed electrostatic interactions in the Fyn SH3 domain

(2011) Arash Zarrine-Afsar et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

A Rigidifying Salt-Bridge Favors the Activity of Thermophilic Enzyme at High Temperatures at the Expense of Low-Temperature Activity

(2011) Sonia Y. Lam et al.   PLOS BIOLOGY

Three amino acid changes contribute markedly to the thermostability of β-glucosidase BglC from Thermobifida fusca

(2010) Xiao-Qiong Pei et al.   BIORESOURCE TECHNOLOGY

Thermostabilization of Bacillus circulans xylanase via computational design of a flexible surface cavity

(2010) Jeong Chan Joo et al.   JOURNAL OF BIOTECHNOLOGY

Protein stability and enzyme activity at extreme biological temperatures

(2010) Georges Feller   JOURNAL OF PHYSICS-CONDENSED MATTER

Remarkable improvements of a neutral protease activity and stability share the same structural origins

(2010) S. M. Asghari et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A chemically modified α-amylase with a molten-globule state has entropically driven enhanced thermal stability†

(2010) Khawar Sohail Siddiqui et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The structure of a glycogen phosphorylase glucopyranose spirohydantoin complex at 1.8 Å resolution and 100 K: The role of the water structure and its contribution to binding

(2010) Mary Gregoriou et al.   PROTEIN SCIENCE

A novel approach for enhancing the catalytic efficiency of a protease at low temperature: Reduction in substrate inhibition by chemical modification

(2009) Khawar Sohail Siddiqui et al.   BIOTECHNOLOGY AND BIOENGINEERING

Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution

(2009) Eugene Rha et al.   FEBS Journal

Cold adaptation of enzymes: Structural, kinetic and microcalorimetric characterizations of an aminopeptidase from the Arctic psychrophile Colwellia psychrerythraea and of human leukotriene A4 hydrolase

(2008) Adrienne L. Huston et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Bioinformatic method for protein thermal stabilization by structural entropy optimization

(2008) E. Bae et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The GAP arginine finger movement into the catalytic site of Ras increases the activation entropy

(2008) C. Kotting et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA