A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications

(2018) Dominik Schumacher et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Identification of VAPA and VAPB as Kv2 channel-interacting proteins defining endoplasmic reticulum-plasma membrane junctions in mammalian brain neurons

(2018) Michael Kirmiz et al.   JOURNAL OF NEUROSCIENCE

Reporter–nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents

(2018) Masahito Yamagata et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons

(2018) Hannah I. Bishop et al.   Frontiers in Molecular Neuroscience

Remodeling neuronal ER–PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels

(2018) Michael Kirmiz et al.   MOLECULAR BIOLOGY OF THE CELL

Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses

(2018) Souvik Modi et al.   Nanoscale

Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons

(2018) Manuel Maidorn et al.   mAbs

Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

(2018) Tao Fang et al.   NATURE METHODS

Towards an Understanding of Synapse Formation

(2018) Thomas C. Südhof   NEURON

Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain–specific functions during invadopodium formation and cancer cell invasion

(2017) Laurence Bertier et al.   FASEB JOURNAL

Identification and characterization of Nanobodies targeting the EphA4 receptor

(2017) Lies Schoonaert et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies

(2017) Tino Pleiner et al.   JOURNAL OF CELL BIOLOGY

Allosteric nanobodies uncover a role of hippocampal mGlu2 receptor homodimers in contextual fear consolidation

(2017) Pauline Scholler et al.   Nature Communications

Hippocampal Regulation of Postsynaptic Density Homer1 by Associative Learning

(2017) Nicholas E. Clifton et al.   NEURAL PLASTICITY

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

(2017) Els Beghein et al.   Frontiers in Immunology

Nanobodies as therapeutics: big opportunities for small antibodies

(2016) Sophie Steeland et al.   DRUG DISCOVERY TODAY

Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles

(2016) Merja Joensuu et al.   JOURNAL OF CELL BIOLOGY

Developing high-quality mouse monoclonal antibodies for neuroscience research – approaches, perspectives and opportunities

(2016) Belvin Gong et al.   New Biotechnology

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

(2016) Ingrid Chamma et al.   Nature Communications

Nanobodies as Versatile Tools to Understand, Diagnose, Visualize and Treat Cancer

(2016) Isabel Van Audenhove et al.   EBioMedicine

A new survivin tracer tracks, delocalizes and captures endogenous survivin at different subcellular locations and in distinct organelles

(2016) Els Beghein et al.   Scientific Reports

Camelid nanobodies: killing two birds with one stone

(2015) Aline Desmyter et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons

(2015) H. I. Bishop et al.   JOURNAL OF NEUROSCIENCE

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Molecular Profiling of Neurons Based on Connectivity

(2014) Mats I. Ekstrand et al.   CELL

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

A Nanobody-Based System Using Fluorescent Proteins as Scaffolds for Cell-Specific Gene Manipulation

(2013) Jonathan C.Y. Tang et al.   CELL

Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging

(2013) A. Szymborska et al.   SCIENCE

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Benefits and Pitfalls of Secondary Antibodies: Why Choosing the Right Secondary Is of Primary Importance

(2012) Colleen F. Manning et al.   PLoS One

Novel protein scaffolds as emerging therapeutic proteins: from discovery to clinical proof-of-concept

(2012) Thierry Wurch et al.   TRENDS IN BIOTECHNOLOGY

Llama VHH antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies

(2009) Claire Perruchini et al.   ACTA NEUROPATHOLOGICA

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth

(2009) Elena Gustchina et al.   VIROLOGY

Single-domain antibodies recognize selectively small oligomeric forms of amyloid β, prevent Aβ-induced neurotoxicity and inhibit fibril formation

(2008) Pierre Lafaye et al.   MOLECULAR IMMUNOLOGY

Fluorescence nanoscopy by ground-state depletion and single-molecule return

(2008) Jonas Fölling et al.   NATURE METHODS

An scFv Intrabody against the Nonamyloid Component of α-Synuclein Reduces Intracellular Aggregation and Toxicity

(2007) Sandra M. Lynch et al.   JOURNAL OF MOLECULAR BIOLOGY