Establishment and application of CRISPR interference to affect sporulation, hydrogen peroxide detoxification, and mannitol catabolism in the methylotrophic thermophile Bacillus methanolicus

Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli

(2019) Tian Tian et al.   ACS Synthetic Biology

Bacteria and archaea on Earth and their abundance in biofilms

(2019) Hans-Curt Flemming et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR interference-mediated metabolic engineering of Corynebacterium glutamicum for homo-butyrate production

(2018) Jinkyung Yoon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic engineering to enhance heterologous production of hyaluronic acid in Bacillus subtilis

(2018) Adam W. Westbrook et al.   METABOLIC ENGINEERING

RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain

(2018) Jaehyun Park et al.   Microbial Cell Factories

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

Heterologous Production of Squalene from Glucose in Engineered Corynebacterium glutamicum Using Multiplex CRISPR Interference and High-Throughput Fermentation

(2018) Jaehyun Park et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

The ahpD gene of Corynebacterium glutamicum plays an important role in hydrogen peroxide-induced oxidative stress response

(2018) Eun-Ji Hong et al.   JOURNAL OF BIOCHEMISTRY

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

(2017) Seong Keun Kim et al.   Microbial Cell Factories

CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

(2017) Brady F. Cress et al.   Microbial Cell Factories

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

(2016) Seong Keun Kim et al.   METABOLIC ENGINEERING

A novel process for obtaining pinosylvin using combinatorial bioengineering in Escherichia coli

(2016) Jing-long Liang et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production

(2016) Marta Irla et al.   Frontiers in Microbiology

Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape

(2015) Marta Irla et al.   BMC GENOMICS

High-Resolution Specificity from DNA Sequencing Highlights Alternative Modes of Lac Repressor Binding

(2014) Z. Zuo et al.   GENETICS

Complete genome sequence of Bacillus methanolicus MGA3, a thermotolerant amino acid producing methylotroph

(2014) Marta Irla et al.   JOURNAL OF BIOTECHNOLOGY

Transcriptional response of Corynebacterium glutamicum ATCC 13032 to hydrogen peroxide stress and characterization of the OxyR regulon

(2014) Johanna Milse et al.   JOURNAL OF BIOTECHNOLOGY

Proteomic analysis of the thermophilic methylotrophBacillus methanolicusMGA3

(2014) Jonas E. N. Müller et al.   PROTEOMICS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Spore formation inBacillus subtilis

(2013) Irene S. Tan et al.   Environmental Microbiology Reports

Genome Sequence of Thermotolerant Bacillus methanolicus: Features and Regulation Related to Methylotrophy and Production of l-Lysine and l-Glutamate from Methanol

(2012) Tonje M. B. Heggeset et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Expanding World of Methylotrophic Metabolism

(2009) Ludmila Chistoserdova et al.   Annual Review of Microbiology

Recent advances in the biological production of mannitol

(2009) Seung Hoon Song et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS