An anti-apoptotic HEK293 cell line provides a robust and high titer platform for transient protein expression in bioreactors

Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells

(2018) Matthew Stuible et al.   JOURNAL OF BIOTECHNOLOGY

Antibodies to watch in 2019

(2018) Hélène Kaplon et al.   mAbs

Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein

(2016) Kai Ding et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells

(2016) Laurence Delafosse et al.   JOURNAL OF BIOTECHNOLOGY

Lactate and glucose concomitant consumption as a self-regulated pH detoxification mechanism in HEK293 cell cultures

(2015) Leticia Liste-Calleja et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Optimization and automation of an end-to-end high throughput microscale transient protein production process

(2015) Aaron B. Bos et al.   BIOTECHNOLOGY AND BIOENGINEERING

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

(2015) Yashas Rajendra et al.   BIOTECHNOLOGY PROGRESS

Application of high-throughput mini-bioreactor system for systematic scale-down modeling, process characterization, and control strategy development

(2015) Vijay Janakiraman et al.   BIOTECHNOLOGY PROGRESS

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

High-throughput miniaturized bioreactors for cell culture process development: Reproducibility, scalability, and control

(2014) Shahid Rameez et al.   BIOTECHNOLOGY PROGRESS

Development of a semi-automated high throughput transient transfection system

(2014) Aaron B. Bos et al.   JOURNAL OF BIOTECHNOLOGY

The therapeutic monoclonal antibody market

(2014) Dawn M Ecker et al.   mAbs

Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production

(2013) Shahram Misaghi et al.   BIOTECHNOLOGY PROGRESS

Use of an anti-apoptotic CHO cell line for transient gene expression

(2013) Nichole F. Macaraeg et al.   BIOTECHNOLOGY PROGRESS

A simple, robust and highly efficient transient expression system for producing antibodies

(2013) Tom Vink et al.   METHODS

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

(2013) David L. Hacker et al.   PROTEIN EXPRESSION AND PURIFICATION

Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors

(2012) Wei-Ting Hsu et al.   CYTOTECHNOLOGY

Feeding lactate for CHO cell culture processes: Impact on culture metabolism and performance

(2011) Jincai Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Overcoming challenges in WAVE bioreactors without feedback controls for pH and dissolved oxygen

(2011) Inn H. Yuk et al.   BIOTECHNOLOGY PROGRESS

Transient gene expression in HEK293 and vero cells immobilised on microcarriers

(2011) Lukas Fliedl et al.   JOURNAL OF BIOTECHNOLOGY

Automation of large scale transient protein expression in mammalian cells

(2011) Yuguang Zhao et al.   JOURNAL OF STRUCTURAL BIOLOGY

A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

(2011) Céline Raymond et al.   METHODS

Recent advances in the production of proteins in insect and mammalian cells for structural biology

(2010) Joanne E. Nettleship et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

BAKandBAXdeletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

(2009) Gregory J. Cost et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development of a generic transient transfection process at 100 L scale

(2008) Ola Tuvesson et al.   CYTOTECHNOLOGY

The Kinetics of Polyethylenimine-Mediated Transfection in Suspension Cultures of Chinese Hamster Ovary Cells

(2008) Martin Bertschinger et al.   MOLECULAR BIOTECHNOLOGY

Apoptosis: controlled demolition at the cellular level

(2007) Rebecca C. Taylor et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Transient Gene Expression in Suspension HEK-293 Cells: Application to Large-Scale Protein Production

(2005) Lucia Baldi et al.   BIOTECHNOLOGY PROGRESS