Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi

Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi

(2018) Bruno A.A. Romagnoli et al.   ACTA TROPICA

Effective gene delivery to Trypanosoma cruzi epimastigotes through nucleofection

(2017) Lisandro Pacheco-Lugo et al.   PARASITOLOGY INTERNATIONAL

Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of Trypanosoma cruzi

(2017) Miguel A. Chiurillo et al.   mBio

Host triacylglycerols shape the lipidome of intracellular trypanosomes and modulate their growth

(2017) Felipe Gazos-Lopes et al.   PLoS Pathogens

Modulation of host central carbon metabolism and in situ glucose uptake by intracellular Trypanosoma cruzi amastigotes

(2017) Sheena Shah-Simpson et al.   PLoS Pathogens

Gene expression in Kinetoplastids

(2016) CE Clayton   CURRENT OPINION IN MICROBIOLOGY

CRISPR/Cas9-mediated endogenous C-terminal Tagging ofTrypanosoma cruziGenes Reveals the Acidocalcisome Localization of the Inositol 1,4,5-Trisphosphate Receptor

(2016) Noelia Lander et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome Editing by CRISPR/Cas9: A Game Change in the Genetic Manipulation of Protists

(2016) Noelia Lander et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Transcriptome Remodeling in Trypanosoma cruzi and Human Cells during Intracellular Infection

(2016) Yuan Li et al.   PLoS Pathogens

Drug Delivery Approaches in Addressing Clinical Pharmacology-Related Issues: Opportunities and Challenges

(2015) Hong Wen et al.   AAPS Journal

Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction

(2015) Thaise Lara Teixeira et al.   Parasites & Vectors

CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

(2015) Noelia Lander et al.   mBio

Automated High-Content Assay for Compounds Selectively Toxic to Trypanosoma cruzi in a Myoblastic Cell Line

(2015) Julio Alonso-Padilla et al.   PLoS Neglected Tropical Diseases

Development and Validation of a Novel Leishmania donovani Screening Cascade for High-Throughput Screening Using a Novel Axenic Assay with High Predictivity of Leishmanicidal Intracellular Activity

(2015) Andrea Nühs et al.   PLoS Neglected Tropical Diseases

Transient transfection and expression of foreign and endogenous genes in the intracellular stages of Trypanosoma cruzi

(2015) Prasad K. Padmanabhan et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Comparison of a High-Throughput High-Content Intracellular Leishmania donovani Assay with an Axenic Amastigote Assay

(2013) Manu De Rycker et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruzi

(2013) Monica Mendes Kangussu-Marcolino et al.   BMC MICROBIOLOGY

Host Metabolism Regulates Intracellular Growth of Trypanosoma cruzi

(2013) Kacey L. Caradonna et al.   Cell Host & Microbe

Optimizing Solubility and Permeability of a Biopharmaceutics Classification System (BCS) Class 4 Antibiotic Drug Using Lipophilic Fragments Disturbing the Crystal Lattice

(2013) Ulrika Tehler et al.   JOURNAL OF MEDICINAL CHEMISTRY

Extracellular amastigotes ofTrypanosoma cruziare potent inducers of phagocytosis in mammalian cells

(2012) Maria Cecilia Fernandes et al.   CELLULAR MICROBIOLOGY

Development of Luciferase Expressing Leishmania donovani Axenic Amastigotes as Primary Model for In Vitro Screening of Antileishmanial Compounds

(2012) Ravinder et al.   CURRENT MICROBIOLOGY

Trypanosoma cruzi: Role of δ-Amastin on Extracellular Amastigote Cell Invasion and Differentiation

(2012) Mário C. Cruz et al.   PLoS One

Quantitative proteome profiling informs on phenotypic traits that adapt Leishmania donovani for axenic and intracellular proliferation

(2011) Pascale Pescher et al.   CELLULAR MICROBIOLOGY

Intramolecular hydrogen bonding to improve membrane permeability and absorption in beyond rule of five chemical space

(2011) Alexander Alex et al.   MedChemComm

Large Urban Outbreak of Orally Acquired Acute Chagas Disease at a School in Caracas, Venezuela

(2010) Belkisyolé Alarcón de Noya et al.   JOURNAL OF INFECTIOUS DISEASES

Chagas disease 101

(2010) Julie Clayton   NATURE

Current and developing therapeutic agents in the treatment of Chagas disease

(2010) Werner Apt   Drug Design Development and Therapy

The steady-state transcriptome of the four major life-cycle stages of Trypanosoma cruzi

(2009) Todd A Minning et al.   BMC GENOMICS

Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum

(2009) Annie Rochette et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Rapid fluorescent assay for screening drugs on Leishmania amastigotes

(2008) Orly Shimony et al.   JOURNAL OF MICROBIOLOGICAL METHODS