Quantitative collision-induced unfolding differentiates model antibody-drug conjugates

The growing role of structural mass spectrometry in the discovery and development of therapeutic antibodies

(2018) Yuwei Tian et al.   ANALYST

Signature of Antibody Domain Exchange by Native Mass Spectrometry and Collision-Induced Unfolding

(2018) Yasunori Watanabe et al.   ANALYTICAL CHEMISTRY

An Online Four-Dimensional HIC×SEC-IM×MS Methodology for Proof-of-Concept Characterization of Antibody Drug Conjugates

(2018) Anthony Ehkirch et al.   ANALYTICAL CHEMISTRY

Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies

(2018) Oscar Hernandez-Alba et al.   ANALYTICAL CHEMISTRY

Ion mobility in the pharmaceutical industry: an established biophysical technique or still niche?

(2018) Iain DG Campuzano et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Collision induced unfolding of isolated proteins in the gas phase: past, present, and future

(2018) Sugyan M Dixit et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Collision induced unfolding detects subtle differences in intact antibody glycoforms and associated fragments

(2018) Yuwei Tian et al.   INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

Influence of glycan modification on IgG1 biochemical and biophysical properties

(2018) Jake W. Pawlowski et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Current possibilities of liquid chromatography for the characterization of antibody-drug conjugates

(2018) Balázs Bobály et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Native Mass Spectrometry, Ion mobility, and Collision-Induced Unfolding Categorize Malaria Antigen/Antibody Binding

(2017) Yining Huang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches

(2017) Yang Yang et al.   mAbs

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

(2017) Thomas Botzanowski et al.   mAbs

Characterization of Intact Antibody Drug Conjugate Variants Using Microfluidic Capillary Electrophoresis–Mass Spectrometry

(2016) Erin A. Redman et al.   ANALYTICAL CHEMISTRY

Antibody-drug conjugates—an emerging class of cancer treatment

(2016) Nikolaos Diamantis et al.   BRITISH JOURNAL OF CANCER

Evaluation of Ion Mobility-Mass Spectrometry for Comparative Analysis of Monoclonal Antibodies

(2016) Carly N. Ferguson et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Structural Characterization of a Monoclonal Antibody–Maytansinoid Immunoconjugate

(2015) Quanzhou Luo et al.   ANALYTICAL CHEMISTRY

Collision Induced Unfolding of Intact Antibodies: Rapid Characterization of Disulfide Bonding Patterns, Glycosylation, and Structures

(2015) Yuwei Tian et al.   ANALYTICAL CHEMISTRY

CIUSuite: A Quantitative Analysis Package for Collision Induced Unfolding Measurements of Gas-Phase Protein Ions

(2015) Joseph D. Eschweiler et al.   ANALYTICAL CHEMISTRY

Tandem Native Mass-Spectrometry on Antibody–Drug Conjugates and Submillion Da Antibody–Antigen Protein Assemblies on an Orbitrap EMR Equipped with a High-Mass Quadrupole Mass Selector

(2015) Andrey Dyachenko et al.   ANALYTICAL CHEMISTRY

Conformation and Dynamics of Interchain Cysteine-Linked Antibody-Drug Conjugates as Revealed by Hydrogen/Deuterium Exchange Mass Spectrometry

(2014) Lucy Yan Pan et al.   ANALYTICAL CHEMISTRY

Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment

(2014) François Debaene et al.   ANALYTICAL CHEMISTRY

Measurement of in Vivo Drug Load Distribution of Cysteine-Linked Antibody–Drug Conjugates Using Microscale Liquid Chromatography Mass Spectrometry

(2014) Shawna Mae Hengel et al.   ANALYTICAL CHEMISTRY

Dynamics of Intact Immunoglobulin G Explored by Drift-Tube Ion-Mobility Mass Spectrometry and Molecular Modeling

(2014) Kamila J. Pacholarz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Antibody-Drug Conjugates: An Emerging Concept in Cancer Therapy

(2014) Ravi V. J. Chari et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Statistical Modeling of the Drug Load Distribution on Trastuzumab Emtansine (Kadcyla), a Lysine-Linked Antibody Drug Conjugate

(2014) Michael T. Kim et al.   BIOCONJUGATE CHEMISTRY

A Hydrodynamic Comparison of Solution and Gas Phase Proteins and Their Complexes

(2014) Dominic Hewitt et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Approaches to Interchain Cysteine-Linked ADC Characterization by Mass Spectrometry

(2014) John F. Valliere-Douglass et al.   MOLECULAR PHARMACEUTICS

From Protein Complexes to Subunit Backbone Fragments: A Multi-stage Approach to Native Mass Spectrometry

(2013) Mikhail E. Belov et al.   ANALYTICAL CHEMISTRY

Site-specific antibody drug conjugates for cancer therapy

(2013) Siler Panowski et al.   mAbs

Native Intact Mass Determination of Antibodies Conjugated with Monomethyl Auristatin E and F at Interchain Cysteine Residues

(2012) John F. Valliere-Douglass et al.   ANALYTICAL CHEMISTRY

The impact of glycosylation on monoclonal antibody conformation and stability

(2012) Kai Zheng et al.   mAbs

Impact of linker and conjugation chemistry on antigen binding, Fc receptor binding and thermal stability of model antibody-drug conjugates

(2012) Mauro Acchione et al.   mAbs

High-sensitivity Orbitrap mass analysis of intact macromolecular assemblies

(2012) Rebecca J Rose et al.   NATURE METHODS

Characterizing the resolution and accuracy of a second-generation traveling-wave ion mobility separator for biomolecular ions

(2011) Yueyang Zhong et al.   ANALYST

Analytical methods for physicochemical characterization of antibody drug conjugates

(2011) Aditya Wakankar et al.   mAbs

Collision Cross Sections of Proteins and Their Complexes: A Calibration Framework and Database for Gas-Phase Structural Biology

(2010) Matthew F. Bush et al.   ANALYTICAL CHEMISTRY

Physicochemical Stability of the Antibody−Drug Conjugate Trastuzumab-DM1: Changes due to Modification and Conjugation Processes

(2010) Aditya A. Wakankar et al.   BIOCONJUGATE CHEMISTRY

Fundamentals of Traveling Wave Ion Mobility Spectrometry

(2008) Alexandre A. Shvartsburg et al.   ANALYTICAL CHEMISTRY