Design of fast proteolysis-based signaling and logic circuits in mammalian cells

Programming gene and engineered-cell therapies with synthetic biology

(2018) Tasuku Kitada et al.   SCIENCE

Programmable protein circuits in living cells

(2018) Xiaojing J. Gao et al.   SCIENCE

Modulation of Coiled-Coil Dimer Stability through Surface Residues while Preserving Pairing Specificity

(2017) Igor Drobnak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rewiring human cellular input–output using modular extracellular sensors

(2016) Kelly A Schwarz et al.   Nature Chemical Biology

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

Post-translational control of genetic circuits usingPotyvirusproteases

(2016) Jesus Fernandez-Rodriguez et al.   NUCLEIC ACIDS RESEARCH

Engineering dynamical control of cell fate switching using synthetic phospho-regulons

(2016) Russell M. Gordley et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of soybean mosaic virus as a versatile tool for studying protein–protein interactions in soybean

(2016) Jang-Kyun Seo et al.   Scientific Reports

Protease-based synthetic sensing and signal amplification

(2014) Viktor Stein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Function-Based Mutation-Resistant Synthetic Signaling Device Activated by HIV-1 Proteolysis

(2014) Andreja Majerle et al.   ACS Synthetic Biology

Nucleotide-binding oligomerization domain containing-like receptor family, caspase recruitment domain (CARD) containing 4 (NLRC4) regulates intrapulmonary replication of aerosolized Legionella pneumophila

(2013) William R Berrington et al.   BMC INFECTIOUS DISEASES

Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments

(2013) Helena Gradišar et al.   Nature Chemical Biology

Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

(2013) L. Yi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic Biology: Integrated Gene Circuits

(2011) N. Nandagopal et al.   SCIENCE

Scaffold Proteins: Hubs for Controlling the Flow of Cellular Information

(2011) M. C. Good et al.   SCIENCE

Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity

(2011) F.-S. Liang et al.   Science Signaling

De novo design of orthogonal peptide pairs forming parallel coiled-coil heterodimers

(2010) Helena Gradišar et al.   JOURNAL OF PEPTIDE SCIENCE

A Synthetic Coiled-Coil Interactome Provides Heterospecific Modules for Molecular Engineering

(2010) Aaron W. Reinke et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors

(2009) Sujan S. Shekhawat et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An auxin-based degron system for the rapid depletion of proteins in nonplant cells

(2009) Kohei Nishimura et al.   NATURE METHODS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Structural specificity in coiled-coil interactions

(2008) G GRIGORYAN et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Specific and efficient cleavage of fusion proteins by recombinant plum pox virus NIa protease

(2007) Nuoyan Zheng et al.   PROTEIN EXPRESSION AND PURIFICATION