Review
Biochemistry & Molecular Biology
Irene Lee-Rivera, Edith Lopez, Ana Maria Lopez-Colome
Summary: This review discusses the interactions of protease activated receptors (PARs) with their own family members as well as with other types of receptors. The authors point out that the cross-talk between receptors and signaling pathways is a novel mechanism providing diversity to receptor function and playing important roles in physiology and disease.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Review
Cell Biology
Yagmur Azbazdar, Mustafa Karabicici, Esra Erdal, Gunes Ozhan
Summary: Wnt signaling pathway plays a crucial role in physiological activities and has significant implications in cancer development and progression. By regulating plasma membrane composition and lipid organization, Wnt signaling can be activated or inhibited, leading to the development of more targeted anticancer drugs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Yunpeng Li, Xiaozhu Wang, Liumei Lu, Meng Wang, Yue Zhai, Xiaolu Tai, Diliqingna Dilimulati, Xiaowei Lei, Jing Xu, Cong Zhang, Yanbin Fu, Shen Qu, Qingfeng Li, Chao Zhang
Summary: This study aimed to explore and identify novel GPCR partners of MC3R and MC4R, investigate the pharmacological effects on melanocortin receptors, and validate their downstream signal transduction and membrane translocation effects. Through single cell RNA-seq datasets and co-immunoprecipitation validation, 23 novel GPCR partners interacting with MC3R and 32 with MC4R were identified.
MOLECULAR METABOLISM
(2021)
Article
Multidisciplinary Sciences
Jingyi Zhao, Vincent DiGiacomo, Mariola Ferreras-Gutierrez, Shiva Dastjerdi, Alain Ibanez de Opakua, Jong-Chan Park, Alex Luebbers, Qingyan Chen, Aaron Beeler, Francisco J. Blanco, Mikel Garcia-Marcos
Summary: IGGi-11 selectively inhibits noncanonical activation of heterotrimeric G-protein signaling, blocking tumor cell signaling and inhibiting metastatic cancer cell invasion without interfering with canonical GPCR signaling mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Letter
Medicine, Research & Experimental
Julia Etich, Oliver Semler, Nicola L. Stevenson, Alice Stephan, Roberta Besio, Nadia Garibaldi, Nadine Reintjes, Claudia Dafinger, Max Christoph Liebau, Ulrich Baumann, Matthias Moergelin, Antonella Forlino, David J. Stephens, Christian Netzer, Frank Zaucke, Mirko Rehberg
Summary: This study extends the range of TAPT1 mutations causing osteogenesis imperfecta (OI) and connects extracellular matrix changes to signaling regulation.
EMBO MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Wentong Jiang, Sanduo Zheng
Summary: This study reports the cryoelectron microscopy structures of the GALR1-G(o) and GALR2-G(q) complexes bound to the endogenous ligand galanin or spexin, revealing the structural determinants of GALRs and highlighting the potential role of zinc ion as a modulator of GALR1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Hui Wu, Linyan Zheng, Neng Ling, Liyan Zheng, Yulin Du, Qiang Zhang, Yue Liu, Weihong Tan, Liping Qiu
Summary: Chemically synthetic receptors, such as Ts-pHLIP-Pr, have been developed to equip cells with artificial signaling pathways and enable interaction with the outer world. Through conformational switch of pHLIP, specific proteins can be recruited to trigger downstream signaling cascades upon sensing external pH stimuli. This receptor can be extended to induce T-cell activation via low-pH environment-induced directional movement of cytoplasmic ZAP70.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Endocrinology & Metabolism
Seungheon Lee, Mi Ae Kim, Jong-Moon Park, Keunwan Park, Young Chang Sohn
Summary: This study reveals the TK signaling system in lophotrochozoan mollusks, specifically in the Pacific abalone Haliotis discus hannai (Hdh). The study identifies TK precursors and TK receptor isoforms in Hdh and characterizes Hdh-TK signaling. The findings suggest a possible role for TK peptides in regulating lipid metabolism in the neural and peripheral tissues of abalone.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Taylor P. Light, Delphine Brun, Pablo Guardado-Calvo, Riccardo Pederzoli, Ahmed Haouz, Frank Neipel, Felix A. Rey, Kalina Hristova, Marija Backovic
Summary: The study reveals that the HHV-8 envelope glycoprotein complex H and L binds to EphA2 by mimicking the receptor's cellular ligands, ephrins, and triggers receptor signaling for cytoskeleton remodeling. This provides novel insight into the entry mechanism of HHV-8 and potential therapeutic targets for HHV-8-related diseases.
Article
Multidisciplinary Sciences
Geng Chen, Jun Xu, Asuka Inoue, Maximilian F. Schmidt, Chen Bai, Qiuyuan Lu, Peter Gmeiner, Zheng Liu, Yang Du
Summary: This study reports two structures of the human GPR88-Gi complex, revealing an allosteric ligand involved in the interaction between the receptor and G-protein, and a potential endogenous ligand of GPR88.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Delia I. Fernandez, Marijke J. E. Kuijpers, Johan W. M. Heemskerk
Summary: Platelets are stimulated to increase Ca2+ levels through G-protein coupled receptors or ITAM-linked receptors. There are significant differences in Ca2+ signaling between different receptor types, and simultaneous stimulation of both types of receptors can lead to a highly enforced Ca2+ rise.
Review
Cell Biology
Yiwei Zhou, Jiyong Meng, Chanjuan Xu, Jianfeng Liu
Summary: G protein-coupled receptors (GPCRs) are a large membrane protein family involved in physiological and pathological activities, and various sensors have been developed to monitor conformational changes for high-throughput drug screening purposes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Yun Lim, Ji Yul Kim, Youn Hee Jung, Jae Hoon Lee, Min Seok Baek, Je Hyeong Jung, Ho-Youn Kim, Wookbin Lee, Keunwan Park, Moon-Hyeong Seo
Summary: Studies on glutamine metabolism have revealed its vital role in cellular functions and disease detection. We developed a new Gln sensor, Q-SHINE, which accurately measures Gln concentrations in bio-fluid samples and visualizes and quantifies Gln changes in living cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Sergey A. Vishnivetskiy, Liana D. Weinstein, Chen Zheng, Eugenia V. Gurevich, Vsevolod V. Gurevich
Summary: Arresin-1 shows selectivity for light-activated phosphorylated rhodopsin, and this selectivity is mediated by two structural elements in the molecule. In the crystal structure, there are some residues close to rhodopsin that do not belong to the selectivity mechanism. Mutations in these residues enhance binding to unphosphorylated rhodopsin and increase its selectivity for phosphorylated rhodopsin. This challenges the current model of arrestin-receptor interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sergey A. Vishnivetskiy, Elizabeth K. Huh, Preethi C. Karnam, Samantha Oviedo, Eugenia Gurevich, Vsevolod V. Gurevich
Summary: Arrestins preferentially bind phosphorylated G protein-coupled receptors (GPCRs) through their conserved middle loop, which directly interacts with the bound GPCR. Mutagenesis studies reveal that the middle loop primarily acts as a suppressor of binding to non-preferred forms of the receptor. Certain mutations in the middle loop enhance the binding to unphosphorylated light-activated rhodopsin, making them potential candidates for improving phosphorylation-independent arrestins. However, enhanced forms of arrestin do not bind GPCRs exactly like the wild-type protein, indicating the need for caution when interpreting the structures of arrestin-receptor complexes with different enhanced arrestin mutants and reengineered receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ratna Ghosh, Marilia K. F. de Campos, Jin Huang, Seong K. Huh, Adam Orlowski, Yuan Yang, Ashutosh Tripathi, Aaron Nile, Hsin-Chieh Lee, Marek Dynowski, Helen Schaefer, Tomasz Rog, Marta G. Lete, Hasna Ahyayauch, Alicia Alonso, Ilpo Vattulainen, Tatyana I. Igumenova, Gabriel Schaaf, Vytas A. Bankaitis
MOLECULAR BIOLOGY OF THE CELL
(2015)
Article
Biochemistry & Molecular Biology
Aaron H. Nile, Rami N. Hannoush
NATURE CHEMICAL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Aaron H. Nile, Susmith Mukund, Karen Stanger, Weiru Wang, Rami N. Hannoush
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemistry & Molecular Biology
Aaron H. Nile, Felipe de Sousa e Melo, Susmith Mukund, Robert Piskol, Simon Hansen, Lijuan Zhou, Yingnan Zhang, Yue Fu, Emily B. Gogol, Laszlo G. Komuves, Zora Modrusan, Stephane Angers, Yvonne Franke, Christopher Koth, Wayne J. Fairbrother, Weiru Wang, Frederic J. de Sauvage, Rami N. Hannoush
NATURE CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Ashutosh Tripathi, Aaron H. Nile, Vytas A. Bankaitis
BIOCHEMICAL SOCIETY TRANSACTIONS
(2014)
Article
Biochemistry & Molecular Biology
Carl J. Mousley, Peihua Yuan, Naseem A. Gaur, Kyle D. Trettin, Aaron H. Nile, Stephen J. Deminoff, Brian J. Dewar, Max Wolpert, Jeffrey M. Macdonald, Paul K. Herman, Alan G. Hinnebusch, Vytas A. Bankaitis
Article
Cell Biology
Jihui Ren, Coney Pei-Chen Lin, Manish C. Pathak, Brenda R. S. Temple, Aaron H. Nile, Carl J. Mousley, Mara C. Duncan, Debra M. Eckert, Thomas J. Leiker, Pavlina T. Ivanova, David S. Myers, Robert C. Murphy, H. Alex Brown, Jolien Verdaasdonk, Kerry S. Bloom, Eric A. Ortlund, Aaron M. Neiman, Vytas A. Bankaitis
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Biochemistry & Molecular Biology
Aaron H. Nile, Ashutosh Tripathi, Peihua Yuan, Carl J. Mousley, Sundari Suresh, Iain M. Wallace, Sweety D. Shah, Denise Teotico Pohlhaus, Brenda Temple, Corey Nislow, Guri Giaever, Alexander Tropsha, Ronald W. Davis, Robert P. St. Onge, Vytas A. Bankaitis
NATURE CHEMICAL BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Anna Y. Lee, Robert P. St. Onge, Michael J. Proctor, Iain M. Wallace, Aaron H. Nile, Paul A. Spagnuolo, Yulia Jitkova, Marcela Gronda, Yan Wu, Moshe K. Kim, Kahlin Cheung-Ong, Nikko P. Torres, Eric D. Spear, Mitchell K. L. Han, Ulrich Schlecht, Sundari Suresh, Geoffrey Duby, Lawrence E. Heisler, Anuradha Surendra, Eula Fung, Malene L. Urbanus, Marinella Gebbia, Elena Lissina, Molly Miranda, Jennifer H. Chiang, Ana Maria Aparicio, Mahel Zeghouf, Ronald W. Davis, Jacqueline Cherfils, Marc Boutry, Chris A. Kaiser, Carolyn L. Cummins, William S. Trimble, Grant W. Brown, Aaron D. Schimmer, Vytas A. Bankaitis, Corey Nislow, Gary D. Bader, Guri Giaever
Article
Biophysics
Taichi Sugiura, Chisato Takahashi, Yusuke Chuma, Masakazu Fukuda, Makiko Yamada, Ukyo Yoshida, Hiroyuki Nakao, Keisuke Ikeda, Danish Khan, Aaron H. Nile, Vytas A. Bankaitis, Minoru Nakano
BIOPHYSICAL JOURNAL
(2019)
Article
Chemistry, Medicinal
Simon Hansen, Aaron H. Nile, Shrenik C. Mehta, Jakob Fuhrmann, Rami N. Hannoush
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Taichi Sugiura, Hiroyuki Nakao, Keisuke Ikeda, Danish Khan, Aaron H. Nile, Vytas A. Bankaitis, Minoru Nakano
Summary: The study focuses on lipid packing of membranes and demonstrates that packing defects of membranes promote Sec14 membrane binding and phospholipid transfer. Increased levels of phospholipid unsaturation accelerate Sec14-mediated phosphatidylcholine transfer.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Review
Biochemistry & Molecular Biology
Danish Khan, Aaron H. Nile, Ashutosh Tripathi, Vytas A. Bankaitis
Summary: The emergence of fungal superbugs resistant to current anti-fungal agents is diminishing our ability to treat infections effectively, highlighting the urgent need for new classes of anti-fungal drugs and targets. Recent evidence suggests that Sec14-like phosphatidylinositol transfer proteins (PITPs) could be unexplored portals for selectively disrupting phosphoinositide signaling in virulent fungi, offering exciting potential for next generation Sec14 inhibitors. Development of refined Sec14-directed inhibitors could enhance the chemical weaponry available for combating fungal pathogens and their interactions with human hosts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jina Yun, Simon Hansen, Otto Morris, David T. Madden, Clare Peters Libeu, Arjun J. Kumar, Cameron Wehrfritz, Aaron H. Nile, Yingnan Zhang, Lijuan Zhou, Yuxin Liang, Zora Modrusan, Michelle B. Chen, Christopher C. Overall, David Garfield, Judith Campisi, Birgit Schilling, Rami N. Hannoush, Heinrich Jasper
Summary: Cellular senescence and the senescence-associated secretory phenotype (SASP) play a role in aging and age-related diseases. The inflammation caused by SASP is believed to contribute to tissue dysfunction in aging and diseased animals. Research using intestinal organoids as a model has found that SASP factors released by senescent fibroblasts disturb stem cell activity and differentiation, leading to impaired crypt formation. It has been discovered that the N-terminal domain of Ptk7, which is secreted by senescent cells, activates non-canonical Wnt/Ca2+ signaling through FZD7 in intestinal stem cells (ISCs), disrupting symmetry breaking and stem cell differentiation. This study provides insight into the mechanism by which cellular senescence contributes to tissue dysfunction in aging and disease.
NATURE COMMUNICATIONS
(2023)