Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production

Exploring three different expression systems for recombinant expression of globins: Escherichia coli , Pichia pastoris and Spodoptera frugiperda

(2018) An Bracke et al.   ANALYTICAL BIOCHEMISTRY

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution

(2018) Javier Viña-Gonzalez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

(2018) Thomas Vogl et al.   BIOTECHNOLOGY AND BIOENGINEERING

Superior proteina titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris

(2018) Roland Prielhofer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

(2018) Zhiliang Yang et al.   Biotechnology for Biofuels

Production and cleavage of a fusion protein of porcine trypsinogen and enhanced green fluorescent protein (EGFP) in Pichia pastoris

(2018) Hana Raschmanová et al.   FOLIA MICROBIOLOGICA

A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides

(2018) Qian Sun et al.   FOOD CHEMISTRY

Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture

(2018) Vikas Kumar Dagar et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Impact of the cultivation strategy on resveratrol production from glucose in engineered Corynebacterium glutamicum

(2018) Adelaide Braga et al.   JOURNAL OF BIOTECHNOLOGY

High-Level Expression of a Thermally Stable Alginate Lyase Using Pichia pastoris, Characterization and Application in Producing Brown Alginate Oligosaccharide

(2018) Haifeng Li et al.   Marine Drugs

Metabolic engineering of Pichia pastoris GS115 for enhanced pentose phosphate pathway (PPP) flux toward recombinant human interferon gamma (hIFN-γ) production

(2018) Ashish A. Prabhu et al.   MOLECULAR BIOLOGY REPORTS

Morphology and rheological behaviour of Yarrowia lipolytica : Impact of dissolved oxygen level on cell growth and lipid composition

(2018) Sara Magdouli et al.   PROCESS BIOCHEMISTRY

High-level expression of a novel protease-resistant α-galactosidase from Thielavia terrestris

(2018) Yu Liu et al.   PROCESS BIOCHEMISTRY

High-level production of a fungal β-glucosidase with application potentials in the cost-effective production of Trichoderma reesei cellulase

(2018) Ying Xia et al.   PROCESS BIOCHEMISTRY

Improved production and characterization of Volvariella volvacea Endoglucanase 1 expressed in Pichia pastoris

(2018) Ling Li et al.   PROTEIN EXPRESSION AND PURIFICATION

Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris

(2018) Thomas Vogl et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Advanced monitoring and control of pharmaceutical production processes with Pichia pastoris by using Raman spectroscopy and multivariate calibration methods

(2017) Jan-Patrick Voss et al.   ENGINEERING IN LIFE SCIENCES

Thermo-alkali-stable α-carbonic anhydrase of Bacillus halodurans: heterologous expression in Pichia pastoris and applicability in carbon sequestration

(2017) Shazia Faridi et al.   ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

Extremely low frequency magnetic field enhances glucose oxidase expression in Pichia pastoris GS115

(2017) Alireza Madjid Ansari et al.   ENZYME AND MICROBIAL TECHNOLOGY

An improved process for the production of highly purified recombinant thaumatin tagged-variants

(2017) Robert D. Healey et al.   FOOD CHEMISTRY

Enhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chaperones and fermenting in fed-batch mode

(2017) Qing-Hua Wang et al.   JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH

Differences in growth physiology and aggregation of Pichia pastoris cells between solid-state and submerged fermentations under aerobic conditions

(2017) Jorge Lima-Pérez et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Biotransformation of menadione to its prenylated derivative MK-3 using recombinant Pichia pastoris

(2017) Zhemin Li et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

(2017) Esther Giesselmann et al.   Microbial Cell Factories

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

(2017) Jonas Burgard et al.   Microbial Cell Factories

A comparative study of maltooligosyltrehalose synthase from Sulfolobus acidocaldarius expressed in Pichia pastoris and Escherichia coli

(2017) Chang Han et al.   PROCESS BIOCHEMISTRY

Transcriptional analysis for carbon metabolism and kinetic modeling for heterologous proteins productions by Pichia pastoris in induction process with methanol/sorbitol co-feeding

(2017) Luqiang Jia et al.   PROCESS BIOCHEMISTRY

Expression, fermentation, purification and lyophilisation of recombinant Subtilisin QK in Pichia pastoris

(2017) Kangping Zhou et al.   PROCESS BIOCHEMISTRY

Process development for production and purification of the Schistosoma mansoni Sm14 antigen

(2017) Leonardo Damasceno et al.   PROTEIN EXPRESSION AND PURIFICATION

Recombinant expression, purification and antimicrobial activity of a novel antimicrobial peptide PaDef in Pichia pastoris

(2017) De-Mei Meng et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level expression of l-glutamate oxidase in Pichia pastoris using multi-copy expression strains and high cell density cultivation

(2017) Wang YaPing et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression of the enzymatically active legumain-like cysteine proteinase TvLEGU-1 of Trichomonas vaginalis in Pichia pastoris

(2017) Gerardo Reséndiz-Cardiel et al.   PROTEIN EXPRESSION AND PURIFICATION

Pichia pastoris Alcohol Oxidase 1 (AOX1 ) Core Promoter Engineering by High Resolution Systematic Mutagenesis

(2017) Rui M. C. Portela et al.   Biotechnology Journal

Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

(2017) Deepak Parashar et al.   Frontiers in Microbiology

Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

(2017) Jinjia Wang et al.   Scientific Reports

On-line methanol sensor system development for recombinant human serum albumin production by Pichia pastoris

(2016) Chongchittapiban Panchiga et al.   AFRICAN JOURNAL OF BIOTECHNOLOGY

Enhanced truncated-t-PA (CT-b) expression in high-cell-density fed-batch cultures of Pichia pastoris through optimization of a mixed feeding strategy by response surface methodology

(2016) Mohammad Reza Kazemali et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

The vitamin-sensitive promoter PTHI11enables pre-defined autonomous induction of recombinant protein production inPichia pastoris

(2016) Nils Landes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Heterologous expression of the plant cysteine protease bromelain and its inhibitor inPichia pastoris

(2016) Nora Luniak et al.   BIOTECHNOLOGY PROGRESS

GAP promoter-based fed-batch production of highly bioactive core streptavidin byPichia pastoris

(2016) Jakob Michael Müller et al.   BIOTECHNOLOGY PROGRESS

Enhancing production of lipase MAS1 from marine Streptomyces sp. strain in Pichia pastoris by chaperones co-expression

(2016) Dongming Lan et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate

(2016) F. Carly et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A novel methanol-free Pichia pastoris system for recombinant protein expression

(2016) Wei Shen et al.   Microbial Cell Factories

Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

(2016) Andrea Camattari et al.   Microbial Cell Factories

Enhancing granulocyte colony-stimulating factor expression in Pichia pastoris through fusion with human serum albumin

(2016) Moolchand Sigar et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Expression and purification of the kinase domain of PINK1 in Pichia pastoris

(2016) Daichao Wu et al.   PROTEIN EXPRESSION AND PURIFICATION

Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

(2016) Wan-Cang Liu et al.   Scientific Reports

Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris

(2016) Alexander Rodríguez-López et al.   Scientific Reports

Expression and Control of Codon-Optimized Granulocyte Colony-Stimulating Factor in Pichia pastoris

(2015) Nitu Maity et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improving Performance and Operational Stability of Porcine Interferon-α Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding

(2015) Min-Jie Gao et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

(2015) Zhen Kang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Over-expression of activeCandida rugosa lip1inPichia pastorisvia high cell-density fermentation and its application to resolve racemic ibuprofen

(2015) Xiao Xiao et al.   BIOCATALYSIS AND BIOTRANSFORMATION

Real-time monitoring of glycerol and methanol to enhance antibody production in industrial Pichia pastoris bioprocesses

(2015) Sehoon Kim et al.   BIOCHEMICAL ENGINEERING JOURNAL

Development and in silico analysis of a new nitrogen-limited feeding strategy for fed-batch cultures of Pichia pastoris based on a simple pH-control system

(2015) Zahra Azimzadeh Irani et al.   BIOCHEMICAL ENGINEERING JOURNAL

Maximizing biomass concentration in baker’s yeast process by using a decoupled geometric controller for substrate and dissolved oxygen

(2015) Viki R. Chopda et al.   BIORESOURCE TECHNOLOGY

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

A macrokinetic model-based comparative meta-analysis of recombinant protein production byPichia pastorisunderAOX1promoter

(2015) José Manuel Barrigon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

(2015) Cheng Li et al.   BMC BIOTECHNOLOGY

Molecular Oxygen and Reactive Oxygen Species in Bread-making Processes: Scarce, but Nevertheless Important

(2015) Karolien Decamps et al.   CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION

Overproduction, purification, and property analysis of an extracellular recombinant fructosyltransferase

(2015) Wenwen Guo et al.   EUROPEAN FOOD RESEARCH AND TECHNOLOGY

Selection of suitably non-repressing carbon sources for expression of alcohol oxidase isozyme promoters in the methylotrophic yeast Pichia methanolica

(2015) Tomoyuki Nakagawa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Methanol feeding strategy design enhances recombinant human growth hormone production byPichia pastoris

(2015) Hande Güneş et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Pilot studies on scale-up biocatalysis of 7-β-xylosyl-10-deacetyltaxol and its analogues by an engineered yeast

(2015) Wan-Cang Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Co-feeding strategy to enhance phytase production in Pichia pastoris

(2014) Jean Bernard Ndayambaje et al.   AFRICAN JOURNAL OF BIOTECHNOLOGY

High-Level Extracellular Production of Glucose Oxidase by Recombinant Pichia Pastoris Using a Combined Strategy

(2014) Lei Gu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance

(2014) Özge Ata et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Production of a sterol esterase fromOphiostomapiceae in batch and fed-batch bioprocesses using differentPichiapastorisphenotypes as cell factory

(2014) Víctor Barba Cedillo et al.   BIOTECHNOLOGY PROGRESS

Enhanced heterologous protein production in Pichia pastoris under increased air pressure

(2014) Marlene Lopes et al.   BIOTECHNOLOGY PROGRESS

HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation

(2014) Andrea B. Zepeda et al.   BRAZILIAN JOURNAL OF MICROBIOLOGY

Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy

(2014) Jian Ding et al.   JOURNAL OF BIOTECHNOLOGY

Efficient secretion of inulin fructotransferase in Pichia pastoris using the formaldehyde dehydrogenase 1 promoter

(2014) Rongrong Zhan et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

(2014) Yongjun Zhong et al.   Microbial Cell Factories

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

(2014) Johannes Hemmerich et al.   Microbial Cell Factories

Chitin–glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition

(2014) Bárbara Chagas et al.   New Biotechnology

Bioprocess and downstream optimization of recombinant bovine chymosin B in Pichia (Komagataella) pastoris under methanol-inducible AOXI promoter

(2014) Diego Gabriel Noseda et al.   PROTEIN EXPRESSION AND PURIFICATION

Searching the best operational strategies for Rhizopus oryzae lipase production in Pichia pastoris Mut+ phenotype: Methanol limited or methanol non-limited fed-batch cultures?

(2013) José Manuel Barrigón et al.   BIOCHEMICAL ENGINEERING JOURNAL

Quantitative comparison of dynamic physiological feeding profiles for recombinant protein production with Pichia pastoris

(2013) Oliver Spadiut et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Co-substrate mannitol feeding strategy design in semi-batch production of recombinant human erythropoietin production byPichia pastoris

(2013) Melda Ş. Eskitoros et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

(2013) Hongxing Niu et al.   Microbial Cell Factories

Cloning and Characterization of the Glycoside Hydrolases That Remove Xylosyl Groups from 7-β-xylosyl-10-deacetyltaxol and Its Analogues

(2013) Hai-Li Cheng et al.   MOLECULAR & CELLULAR PROTEOMICS

Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived 13C-labelling data from proteinogenic amino acids

(2013) Joel Jordà et al.   New Biotechnology

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

A simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza A virus Hemagglutinin protein

(2012) T. N. Athmaram et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy

(2011) Min-Jie Gao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A proteomic analysis of the Pichia pastoris secretome in methanol-induced cultures

(2011) Chung-Jr Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production of recombinant proteins by yeast cells

(2011) Eda Çelik et al.   BIOTECHNOLOGY ADVANCES

Improved production of monoclonal antibodies through oxygen-limited cultivation of glycoengineered yeast

(2011) Marina Berdichevsky et al.   JOURNAL OF BIOTECHNOLOGY

Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+

(2011) Leona Paulová et al.   JOURNAL OF BIOTECHNOLOGY

Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review

(2010) Gabriel Potvin et al.   BIOCHEMICAL ENGINEERING JOURNAL

Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis

(2010) Martin Dragosits et al.   BIOTECHNOLOGY PROGRESS

Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

(2009) Zhihao Wang et al.   BIORESOURCE TECHNOLOGY

Improved production of polygalacturonate lyase by combining a pH and online methanol control strategy in a two-stage induction phase with a shift in the transition phase

(2009) Muhammad Salman Qureshi et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Fed-batch methanol feeding strategy for recombinant protein production byPichia pastorisin the presence of co-substrate sorbitol

(2009) Eda Çelik et al.   YEAST

Biomass measurement online: the performance of in situ measurements and software sensors

(2008) Kristiina Kiviharju et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY