Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

Improving Performance and Operational Stability of Porcine Interferon-α Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding

(2015) Min-Jie Gao et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enzymatic production of specifically distributed hyaluronan oligosaccharides

(2015) Panhong Yuan et al.   CARBOHYDRATE POLYMERS

Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes

(2015) Zhen Kang et al.   Bioengineered

Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments

(2014) Lucie Holubova et al.   CARBOHYDRATE POLYMERS

Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species

(2014) Zhen Kang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A Novel Hyaluronidase Produced by Bacillus sp. A50

(2014) Xueping Guo et al.   PLoS One

High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

(2014) Peng Jin et al.   Scientific Reports

Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris

(2013) Zhenmin Ling et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Fusion of an Oligopeptide to the N Terminus of an Alkaline α-Amylase from Alkalimonas amylolytica Simultaneously Improves the Enzyme's Catalytic Efficiency, Thermal Stability, and Resistance to Oxidation

(2013) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced thermal stability and specific activity of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling amphipathic peptides

(2013) Xinyao Lu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements

(2013) Junjiao Zhang et al.   BIORESOURCE TECHNOLOGY

Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris

(2013) Ashok Kumar Prasanna Vadhana et al.   ENZYME AND MICROBIAL TECHNOLOGY

Pichia pastoris: protein production host and model organism for biomedical research

(2013) Brigitte Gasser et al.   Future Microbiology

Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris

(2012) Shuli Liang et al.   BIOTECHNOLOGY LETTERS

Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

(2012) Zhenmin Ling et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

(2012) Ana Leticia Vanz et al.   Microbial Cell Factories

Small surfactant-like peptides can drive soluble proteins into active aggregates

(2012) Bihong Zhou et al.   Microbial Cell Factories

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Protein secretion in Pichia pastoris and advances in protein production

(2011) Leonardo M. Damasceno et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production of recombinant proteins by yeast cells

(2011) Eda Çelik et al.   BIOTECHNOLOGY ADVANCES

Evaluation of the physical and biological properties of hyaluronan and hyaluronan fragments

(2011) Elaine L. Ferguson et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

(2011) Heinrich Lünsdorf et al.   Microbial Cell Factories

Real-time PCR-based determination of gene copy numbers inPichia pastoris

(2010) Sandra Abad et al.   Biotechnology Journal

Hyaluronidases, a group of glycosidases: Current and future perspectives

(2010) Nermeen S. El-Safory et al.   CARBOHYDRATE POLYMERS

Small peptide nanofibers as the matrices of molecular hydrogels for mimicking enzymes and enhancing the activity of enzymes

(2010) Yuan Gao et al.   CHEMICAL SOCIETY REVIEWS

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

(2010) Chandrasekhar Gurramkonda et al.   Microbial Cell Factories

Opportunities and challenges in synthetic oligosaccharide and glycoconjugate research

(2009) Thomas J. Boltje et al.   Nature Chemistry

Lowering induction temperature for enhanced production of polygalacturonate lyase in recombinant Pichia pastoris

(2009) Yun Wang et al.   PROCESS BIOCHEMISTRY

Antibody fragments may be incorrectly processed in the yeast Pichia pastoris

(2008) D. G. Kozlov et al.   BIOTECHNOLOGY LETTERS

Enzymatic hydrogelation to immobilize an enzyme for high activity and stability

(2008) Qigang Wang et al.   Soft Matter

High-yield recombinant expression of the extremophile enzyme, bee hyaluronidase in Pichia pastoris

(2007) Stephan Reitinger et al.   PROTEIN EXPRESSION AND PURIFICATION