CRISPR/Cas9-Based Editing of Streptomyces for Discovery, Characterization, and Production of Natural Products

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment

(2018) Zhen Jie Low et al.   Scientific Reports

Genome Engineering and Modification Toward Synthetic Biology for the Production of Antibiotics

(2017) Xuan Zou et al.   MEDICINAL RESEARCH REVIEWS

Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes

(2017) Lei Li et al.   METABOLIC ENGINEERING

Development of a CRISPR/Cas9-mediated gene-editing tool in Streptomyces rimosus

(2017) Haiyan Jia et al.   MICROBIOLOGY-SGM

CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

(2017) Mingzi M Zhang et al.   Nature Chemical Biology

A dual role for a polyketide synthase in dynemicin enediyne and anthraquinone biosynthesis

(2017) Douglas R. Cohen et al.   Nature Chemistry

Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants

(2017) Zhiwei Qin et al.   Chemical Science

Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster in Streptomyces Species

(2017) Hee-Ju Nah et al.   Frontiers in Microbiology

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Targeted genome editing in the rare actinomycete Actinoplanes sp. SE50/110 by using the CRISPR/Cas9 System

(2016) Timo Wolf et al.   JOURNAL OF BIOTECHNOLOGY

Gifted microbes for genome mining and natural product discovery

(2016) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Engineering microbial hosts for production of bacterial natural products

(2016) Mingzi M. Zhang et al.   NATURAL PRODUCT REPORTS

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA

(2016) Jianting Zhou et al.   NUCLEIC ACIDS RESEARCH

A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis

(2016) W. Tao et al.   Chemical Science

C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1

(2016) Shi-Yuan Li et al.   ACS Synthetic Biology

Multiplexed CRISPR/Cas9- and TAR-Mediated Promoter Engineering of Natural Product Biosynthetic Gene Clusters in Yeast

(2016) Hahk-Soo Kang et al.   ACS Synthetic Biology

Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining

(2015) Xiaoyu Tang et al.   ACS Chemical Biology

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

(2015) Jia-Wang Wang et al.   BIOTECHNIQUES

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Iterative Mechanism of Macrodiolide Formation in the Anticancer Compound Conglobatin

(2015) Yongjun Zhou et al.   CHEMISTRY & BIOLOGY

Culture-independent discovery of natural products from soil metagenomes

(2015) Micah Katz et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Discovery of microbial natural products by activation of silent biosynthetic gene clusters

(2015) Peter J. Rutledge et al.   NATURE REVIEWS MICROBIOLOGY

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

(2015) Nicholas C.O. Lee et al.   NUCLEIC ACIDS RESEARCH

Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters

(2015) Daniel Montiel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A prudent path forward for genomic engineering and germline gene modification

(2015) D. Baltimore et al.   SCIENCE

Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes

(2015) Tilmann Weber et al.   TRENDS IN BIOTECHNOLOGY

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing

(2015) Y. Liu et al.   mBio

Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

(2015) Wenjun Jiang et al.   Nature Communications

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

(2015) Yaojun Tong et al.   ACS Synthetic Biology

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes

(2014) Lorena T. Fernández-Martínez et al.   Scientific Reports

Targeted Capture and Heterologous Expression of the Pseudoalteromonas Alterochromide Gene Cluster in Escherichia coli Represents a Promising Natural Product Exploratory Platform

(2014) Avena C. Ross et al.   ACS Synthetic Biology

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Improvement of gougerotin and nikkomycin production by engineering their biosynthetic gene clusters

(2013) Deyao Du et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways

(2013) Gregory L. Challis   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

(2013) Ari E Friedland et al.   NATURE METHODS

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Recent application of metagenomic approaches toward the discovery of antimicrobials and other bioactive small molecules

(2010) Jacob J Banik et al.   CURRENT OPINION IN MICROBIOLOGY

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Drug Discovery and Natural Products: End of an Era or an Endless Frontier?

(2009) J. W.-H. Li et al.   SCIENCE