Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Engineering aldolases as biocatalysts

(2014) Claire L Windle et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

(2014) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Tuning of Lecitase features via solid-phase chemical modification: Effect of the immobilization protocol

(2014) Cristina Garcia-Galan et al.   PROCESS BIOCHEMISTRY

Stabilizing hyperactivated lecitase structures through physical treatment with ionic polymers

(2014) Jose C.S. dos Santos et al.   PROCESS BIOCHEMISTRY

Protein Engineering For Directed Immobilization

(2013) Erik Steen Redeker et al.   BIOCONJUGATE CHEMISTRY

Polyionic and cysteine-containing fusion peptides as versatile protein tags

(2013) Hauke Lilie et al.   BIOLOGICAL CHEMISTRY

Cationic β-Lactoglobulin Nanoparticles as a Bioavailability Enhancer: Protein Characterization and Particle Formation

(2013) Zi Teng et al.   BIOMACROMOLECULES

Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

(2013) Oveimar Barbosa et al.   BIOMACROMOLECULES

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Stabilizing biocatalysts

(2013) Andreas S. Bommarius et al.   CHEMICAL SOCIETY REVIEWS

Synthesis of ascorbyl oleate by transesterification of olive oil with ascorbic acid in polar organic media catalyzed by immobilized lipases

(2013) Sonia Moreno-Perez et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Strategies for the discovery and engineering of enzymes for biocatalysis

(2013) Timo Davids et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A general strategy for protein immobilization in layered titanates: Polyelectrolyte-assisted self-assembly

(2013) Zhu-Ping Han et al.   ENZYME AND MICROBIAL TECHNOLOGY

Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine

(2013) Cristina Garcia-Galan et al.   ENZYME AND MICROBIAL TECHNOLOGY

Purification of recombinant EGFP by fusion with L2 (252–273) from ribosomal protein L2 using magnetic particles

(2013) Junhua Li et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

(2013) Manfred T. Reetz   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enzymatic hydrolysis of racemic ibuprofen esters using Rhizomucor miehei lipase immobilized on different supports

(2013) Zohreh Habibi et al.   PROCESS BIOCHEMISTRY

Oxidation of phenyl compounds using strongly stable immobilized-stabilized laccase from Trametes versicolor

(2013) Veria Addorisio et al.   PROCESS BIOCHEMISTRY

Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs)

(2013) Sachin Talekar et al.   RSC Advances

Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

(2013) Oveimar Barbosa et al.   RSC Advances

Assessment of immobilized PGA orientation via the LC-MS analysis of tryptic digests of the wild type and its 3K-PGA mutant assists in the rational design of a high-performance biocatalyst

(2012) Immacolata Serra et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2: Design of a heterogeneous D-amino acid oxidase catalyst on porous glass

(2012) Juan M. Bolivar et al.   BIOTECHNOLOGY AND BIOENGINEERING

Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis

(2012) Christopher M. Clouthier et al.   CHEMICAL SOCIETY REVIEWS

Modifying enzyme activity and selectivity by immobilization

(2012) Rafael C. Rodrigues et al.   CHEMICAL SOCIETY REVIEWS

Directed evolution: tailoring biocatalysts for industrial applications

(2012) Ashwani Kumar et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Comparison of magnetic carboxymethyl chitosan nanoparticles and cation exchange resin for the efficient purification of lysine-tagged small ubiquitin-like modifier protease

(2012) Junhua Li et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Evaluation of chemically modified carrier proteins for developing monoclonal antibodies against a clinically relevant mutation of cKIT

(2012) Ricardo Jara-Acevedo et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking

(2012) Jenniffer Cruz et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Solid-phase modification with succinic polyethyleneglycol of aminated lipase B from Candida antarctica: Effect of the immobilization protocol on enzyme catalytic properties

(2012) Mónica Ruiz et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Positively Charged Mini-Protein Zbasic2 As a Highly Efficient Silica Binding Module: Opportunities for Enzyme Immobilization on Unmodified Silica Supports

(2012) Juan M. Bolivar et al.   LANGMUIR

Modulation of the properties of immobilized CALB by chemical modification with 2,3,4-trinitrobenzenesulfonate or ethylendiamine. Advantages of using adsorbed lipases on hydrophobic supports

(2012) Oveimar Barbosa et al.   PROCESS BIOCHEMISTRY

Tailor-made design of penicillin G acylase surface enables its site-directed immobilization and stabilization onto commercial mono-functional epoxy supports

(2012) Valeria Grazu et al.   PROCESS BIOCHEMISTRY

Chemical amination of lipase B from Candida antarctica is an efficient solution for the preparation of crosslinked enzyme aggregates

(2012) Magaly Galvis et al.   PROCESS BIOCHEMISTRY

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

(2011) Rafael C. Rodrigues et al.   ADVANCED SYNTHESIS & CATALYSIS

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Improving Stability and Activity of Cross-linked Enzyme Aggregates Based on Polyethylenimine in Hydrolysis of Fish Oil for Enrichment of Polyunsaturated Fatty Acids

(2011) Jinyong Yan et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

(2011) Roger A. Sheldon   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Polycationic amino acid tags enhance soluble expression of Candida antarctica lipase B in recombinant Escherichia coli

(2011) Hyun-Jung Jung et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non-Covalent Attachment to Anionic Supports usingZbasic2Module

(2011) Johanna Wiesbauer et al.   ChemCatChem

Activity, stability and inhibition of a bioactive paper prepared by large-scale coating of laccase microcapsules

(2011) M.P. Guerrero et al.   CHEMICAL ENGINEERING SCIENCE

Why does the silica-binding protein “Si-tag” bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces

(2011) Takeshi Ikeda et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Full enzymatic hydrolysis of commercial sucrose laurate by immobilized-stabilized derivatives of lipase from Thermomyces lanuginosa

(2011) Marzia Marciello et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

New opportunities for biocatalysis: driving the synthesis of chiral chemicals

(2011) Gao-Wei Zheng et al.   CURRENT OPINION IN BIOTECHNOLOGY

Chemical modification in the creation of novel biocatalysts

(2011) Alba Díaz-Rodríguez et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

(2011) Don A. Cowan et al.   ENZYME AND MICROBIAL TECHNOLOGY

Electrochemically synthesized polyaniline as support for lipase immobilization

(2011) Dejan Bezbradica et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Hen egg white as a feeder protein for lipase immobilization

(2011) Joceline J. Karimpil et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Preparation of cross-linked enzyme aggregates of l-aminoacylase via co-aggregation with polyethyleneimine

(2011) Bhalchandra K. Vaidya et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Modulation of the Selectivity of Immobilized Lipases by Chemical and Physical Modifications: Release of Omega-3 Fatty Acids from Fish Oil

(2011) Gloria Fernández-Lorente et al.   JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY

Activity, conformation and thermal stability of laccase and glucose oxidase in poly(ethyleneimine) microcapsules for immobilization in paper

(2011) Yufen Zhang et al.   PROCESS BIOCHEMISTRY

Effect of solid-phase chemical modification on the features of the lipase from Thermomyces lanuginosus

(2011) Magaly Galvis et al.   PROCESS BIOCHEMISTRY

Production and solid-phase refolding of human glucagon-like peptide-1 using recombinant Escherichia coli

(2011) Sung-Gun Kim et al.   PROTEIN EXPRESSION AND PURIFICATION

Engineering of Biocatalysts: from Evolution to Creation

(2011) Maureen B. Quin et al.   ACS Catalysis

Biocatalysis: Synthesis of Key Intermediates for Development of Pharmaceuticals

(2011) Ramesh N. Patel   ACS Catalysis

Preparation of cross-linked aggregates of aminoacylase from Aspergillus melleus by using bovine serum albumin as an inert additive

(2010) Tao Dong et al.   BIORESOURCE TECHNOLOGY

Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine

(2010) Zaida Cabrera et al.   CATALYSIS COMMUNICATIONS

Protein Chemical Modification on Endogenous Amino Acids

(2010) Emmanuel Baslé et al.   CHEMISTRY & BIOLOGY

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

(2010) G. Volpato et al.   CURRENT MEDICINAL CHEMISTRY

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

(2010) Karel Hernandez et al.   ENZYME AND MICROBIAL TECHNOLOGY

Polyethyleneimine–protein interactions and implications on protein stability

(2010) Laura Mazzaferro et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

(2010) Cheng-Liang Huang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Vinyl sulfone functionalized silica: a “ready to use” pre-activated material for immobilization of biomolecules

(2010) Mariano Ortega-Muñoz et al.   JOURNAL OF MATERIALS CHEMISTRY

Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase

(2010) Jiang Pan et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Preparation of dichlorvos–protein complete antigen by Mannich-type reaction

(2010) Qianqian Feng et al.   JOURNAL OF MOLECULAR STRUCTURE

Oriented immobilization and electron transfer to the cytochrome c oxidase

(2010) Christoph Nowak et al.   JOURNAL OF SOLID STATE ELECTROCHEMISTRY

Two step ethanolysis: A simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from Thermomyces lanuginosus

(2010) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

Immobilization–stabilization of glucoamylase: Chemical modification of the enzyme surface followed by covalent attachment on highly activated glyoxyl-agarose supports

(2010) Paulo Waldir Tardioli et al.   PROCESS BIOCHEMISTRY

Effects of the combined use of Thermomyces lanuginosus and Rhizomucor miehei lipases for the transesterification and hydrolysis of soybean oil

(2010) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes

(2009) Juan M. Bolivar et al.   BIOMACROMOLECULES

Immobilization of biomolecules on nanostructured films for biosensing

(2009) José R. Siqueira et al.   BIOSENSORS & BIOELECTRONICS

Advances in enzyme immobilisation

(2009) Dean Brady et al.   BIOTECHNOLOGY LETTERS

Selective Covalent Protein Immobilization: Strategies and Applications

(2009) Lu Shin Wong et al.   CHEMICAL REVIEWS

Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology

(2009) Justin M. Chalker et al.   Chemistry-An Asian Journal

The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment

(2009) Juan M. Bolivar et al.   ENZYME AND MICROBIAL TECHNOLOGY

Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus

(2009) Rafael C. Rodrigues et al.   ENZYME AND MICROBIAL TECHNOLOGY

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

Coupling of Site-Directed Mutagenesis and Immobilization for the Rational Design of More Efficient Biocatalysts: The Case of Immobilized 3G3K PGA fromE.coli

(2009) Immacolata Serra et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Vinyl sulfone: a versatile function for simple bioconjugation and immobilization

(2009) Julia Morales-Sanfrutos et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

(2009) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers

(2009) César A. Godoy et al.   PROCESS BIOCHEMISTRY

Kinetic and Thermodynamic Study of a Chemically Modified Highly Active Xylanase from Scopulariopsis sp.

(2008) Ahmed Jawaad Afzal et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

(2008) Juan M. Bolivar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose

(2008) Gloria Fernandez-Lorente et al.   BIOMACROMOLECULES

Practical application of different enzymes immobilized on sepabeads

(2008) L. Hilterhaus et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Design of Cytotoxic Ribonucleases by Cationization to Enhance Intracellular Protein Delivery

(2008) Junichiro Futami et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Gold nanoparticle-based electrochemical biosensors

(2008) José M. Pingarrón et al.   ELECTROCHIMICA ACTA

Carrier-bound and carrier-free penicillin acylase biocatalysts for the thermodynamically controlled synthesis of β-lactam compounds in organic medium

(2008) Lorena Wilson et al.   ENZYME AND MICROBIAL TECHNOLOGY

Intracellular Delivery of Glutathione S-transferase-fused Proteins into Mammalian Cells by Polyethylenimine-Glutathione Conjugates

(2008) H. Murata et al.   JOURNAL OF BIOCHEMISTRY

Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements

(2008) César Carrasco-López et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transient cell proliferation with polyethylenimine-cationized N-terminal domain of simian virus 40 large T-antigen

(2008) Hitoshi Murata et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

IMMOBILIZATION OF INVERTASE–CHITOSAN CONJUGATE ON HYALURONIC-ACID-MODIFIED CHITIN

(2008) LEISSY GOMEZ et al.   JOURNAL OF FOOD BIOCHEMISTRY

Preparation and characterization of bisphenol A-cationized bovine serum albumin

(2008) Yi Feng et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles

(2008) Chien-Li Wu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Enzyme stability and stabilization—Aqueous and non-aqueous environment

(2008) Padma V. Iyer et al.   PROCESS BIOCHEMISTRY

Enhancement of Novozym-435 catalytic properties by physical or chemical modification

(2008) Zaida Cabrera et al.   PROCESS BIOCHEMISTRY

The realm of penicillin G acylase in β-lactam antibiotics

(2007) Anuj K. Chandel et al.   ENZYME AND MICROBIAL TECHNOLOGY

Polyarginine as a multifunctional fusion tag

(2005) Stephen M. Fuchs et al.   PROTEIN SCIENCE

Thermodynamically Controlled Synthesis of Amide Bonds Catalyzed by Highly Organic Solvent-Resistant Penicillin Acylase Derivatives

(2004) Olga Abian et al.   BIOTECHNOLOGY PROGRESS

Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic

(2002) Olga N. Ilinskaya et al.   PROTEIN SCIENCE