Review
Pharmacology & Pharmacy
Maria Eugenia Meirinhos Cruz, Maria Luisa Corvo, Maria Barbara Martins, Sandra Simoes, Maria Manuela Gaspar
Summary: The concept of drugs has expanded to include macromolecules such as proteins and enzymes as bioagents in pharmaceuticals. Enzymes, in particular, pose challenges in terms of delivery and carrier systems. This review focuses on therapeutic enzymes and their applications, listing examples that have reached the pharmaceutical market. It also addresses problems associated with their clinical use and explores nanotechnological strategies to address these issues. Liposomes, the most well-studied and suitable nanosystem for enzyme delivery in vivo, are discussed as drug delivery systems. Examples of liposomal enzymatic formulations and successful pre-clinical results are extensively discussed.
Article
Chemistry, Medicinal
Kohei Motojima, Abhijit Sen, Yoichi M. A. Yamada, Hiromasa Kaneko
Summary: Catalyst design using machine learning is important for reducing costs and development time in various industrial fields. This study focused on a reaction system involving Buchwald-Hartwig type cross-coupling (BHCC) and Suzuki-Miyaura type cross-coupling (SMCC) reactions. By constructing a machine-learning model considering all experimental conditions, the study aimed to accurately predict product yield for both BHCC and SMCC reactions and design catalysts that achieve target yield and develop novel reactions. Bayesian optimization was used with established variables to design new catalysts and improve reaction selectivity. Additionally, the catalyst design successfully introduced new reactions involving Cu, Rh, and Pt catalysts in a reaction system that previously only reacted with Ni or Pd transition metals.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Review
Chemistry, Multidisciplinary
Valentina G. Matveeva, Lyudmila M. Bronstein
Summary: The performance of biocatalysts developed by immobilizing enzymes on magnetic nanoparticles is advantageous in terms of easy separation, reusability, and savings of materials and energy. Factors such as magnetic support porosity and surface-modifying agents play a significant role in enhancing the biocatalytic performance of immobilized enzymes.
Article
Biochemistry & Molecular Biology
Jennifer Badura, Niel van Wyk, Silvia Brezina, Isak S. Pretorius, Doris Rauhut, Juergen Wendland, Christian von Wallbrunn
Summary: This study reports the development of a tool for genetic modification of Hanseniaspora uvarum, successfully disrupting the HuATF1 gene encoding an alcohol acetyltransferase, laying the foundation for functional gene analysis of this yeast.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Eun-Ji Seo, Myeong-Ju Kim, So-Yeon Park, Seongsoon Park, Deok-Kun Oh, Uwe Bornscheuer, Jin-Byung Park
Summary: The study focused on improving enzyme stability by predicting enzyme oxidative stress pathways, leading to the design of H2O2-resistant enzyme variants that showed enhanced biocatalytic performance. This rational design approach can be applied to various H2O2-forming or utilizing enzymes in biotransformation processes, improving both oxidative stability and transformation efficiency.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Editorial Material
Biotechnology & Applied Microbiology
J. F. A. Valente, F. Sousa, N. Alves
Summary: Chromatography is widely used in industry, but its common supports have limitations in controlling their structural features. Additive manufacturing is emerging as a technology to produce chromatographic supports, potentially improving performance.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Haiyan Yin, Maziar Sedighi Moghaddam, Mikko Tuominen, Andra Dedinaite, Magnus Walinder, Agne Swerin
Summary: This study investigated the effect of prolonged UV irradiation on the performance of superhydrophobized birch and acetylated birch wood. The results showed that the combination of surface modification using silicone nanofilaments and wood acetylation provided good wetting resistance even after prolonged UV exposure. Scanning electron microscopy images revealed that the silicone nanofilaments were still present on the wood samples after UV irradiation. Additionally, the surface-modified wood samples exhibited significant color change and degradation of lignin after UV exposure.
APPLIED SURFACE SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Pritam Giri, Amol D. Pagar, Mahesh D. Patil, Hyungdon Yun
Summary: This review discusses various strategies of chemical modification of enzymes, including traditional methods utilizing reactivities of canonical amino acids and emerging techniques like noncanonical amino acid-mediated 'click' chemistry and dehydroalanine (Dha)-mediated modifications, to improve functional properties and stability of industrially important biocatalysts.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Chenyang Shi, Shiyu Shao, Chuanxin Zong, Jiahao Gu, Zeyu Huang, Qiyu Wang, Bo Hong, Mengran Wang, Zhian Zhang, Jie Li, Yanqing Lai
Summary: This study introduces 4,4'-thiodibenzenethiol (TBBT) as an additive in the electrolyte to suppress the shuttle effect in lithium-sulfur (Li-S) batteries. TBBT captures polysulfides, forming organic sulfide during charge-discharge process, which effectively extends the cycling life of Li-S batteries.
MATERIALS CHEMISTRY FRONTIERS
(2022)
Article
Biotechnology & Applied Microbiology
Natalie Sandlin, Darius Russell Kish, John Kim, Marco Zaccaria, Babak Momeni
Summary: Biological organisms have potential in detoxifying toxins, but challenges exist in identifying suitable candidates and improving them. Computational tools are utilized to discover strains and enzymes that detoxify harmful compounds, focusing on mycotoxins and detoxifying enzymes. Established and novel computational tools complement empirical data to explore detoxification prospects, essential cellular processes, and enhance enzyme performance in bioremediation. Collaboration between computational biologists and bioremediation researchers is encouraged to address open questions in the field.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tamae Seo, Koji Kubota, Hajime Ito
Summary: Mechanochemical synthesis using transition-metal catalysts has advantages such as low solvent waste and short reaction times. However, the direct use of transition-metal catalysts in mechanochemical reactions without modifications limits the development of efficient cross-coupling processes. This study presents a new approach, using mechanochemistry-directed design to develop ligands for Suzuki-Miyaura cross-coupling reactions. The embedding of the ligand into a poly(ethylene glycol) polymer prevents catalyst deactivation and shows high catalytic activity at room temperature.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Dan Li, Rongchun Wang, Ying Ma, Dianyu Yu
Summary: The aim of this study was to investigate the effects of (+)-catechin covalent cross-linking on the physicochemical properties, rheological properties, and oxidative stability of rice bran protein emulsion. The results showed that a concentration of 0.15% (+)-catechin covalent cross-linking facilitated protein structure unfolding, resulting in reduced particle size, enhanced electrostatic repulsion, and improved emulsion stability. Furthermore, the emulsions stabilized by (+)-catechin covalent cross-linking exhibited superior rheological properties and enhanced oxidation stability compared to the control.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Ceramics
Haidong Wu, Wei Liu, Lifu Lin, Yong Chen, Yuerui Xu, Shanghua Wu, Zhenzhong Sun, Di An, Sai Wei, Zhipeng Xie
Summary: Two types of coupling agents, aluminic acid ester and silane coupling agent, were used to modify Al2O3 ceramic powder based on transesterification reactions, solving the poor compatibility problem between ceramic powder and photosensitive resin in Vat photopolymerization. After pressureless sintering, hot isostatic pressing sintering was applied to further enhance the mechanical performance of the Al2O3 ceramic. Additionally, Al2O3 ceramic cutting tools with chip breaker grooves were successfully prepared and demonstrated superior cutting performance compared to commercial ceramic cutting tools.
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
(2023)
Article
Chemistry, Physical
Iulia-Ioana Radoi, Diana Eva Bedolla, Lisa Vaccari, Anamaria Todea, Federico Zappaterra, Alexey Volkov, Lucia Gardossi
Summary: Enzyme immobilization is an important strategy for expanding enzyme applications and promoting greener and more cost-efficient processes. This study investigated the activity of a & omega;-transaminase in organic solvent upon immobilization on different porosity and surface functionalization carriers. The results showed that the most hydrophilic support, which affects the enzyme secondary structure, achieved the highest enzyme activity. This study sheds light on the interaction between process parameters and carrier properties in the rational development of immobilization protocols.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Biology
Jenni M. Prokkola, Eirik R. Asheim, Sergey Morozov, Paul Bangura, Jaakko Erkinaro, Annukka Ruokolainen, Craig R. Primmer, Tutku Aykanat
Summary: This study reveals the impact of genetic and phenotypic structure on life-history variation, using Atlantic salmon as an ideal model. The research found the association between early maturation genotype with higher metabolic rates and aerobic scope, as well as the physiological epistasis between late maturation genotypes.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Materials Science, Multidisciplinary
Natalia S. Veizaga, Gustavo Mendow, Andres Felipe Quintero-Jaime, Angel Berenguer-Murcia, Sergio de Miguel, Emilia Morallon, Diego Cazorla-Amoros
Summary: The study focuses on the synthesis of mono- and bimetallic Pt-based electrocatalysts for ethanol electrooxidation in acidic media. Incorporation of Re promotes bond breaking of intermediates, while addition of Ir reduces electroactive surface area and decreases electrocatalytic performance.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Review
Food Science & Technology
Veymar G. Tacias-Pascacio, Daniel Castaneda-Valbuena, Roberto Fernandez-Lafuente, Angel Berenguer-Murcia, Rocio Meza-Gordillo, Luis-Felipe Gutierrez, Neith Pacheco, Juan C. Cuevas-Bernardino, Teresa Ayora-Talavera
Summary: Mangifera indica fruit (mango) is a highly commercialized fruit globally, with a large amount of waste generated during processing, posing environmental and economic issues for entrepreneurs. However, the development of biotechnological tools has made these wastes valuable for various industries, as they can be used to produce bioactive compounds.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2022)
Review
Biochemistry & Molecular Biology
Roberto Morellon-Sterling, Olga Tavano, Juan M. Bolivar, Angel Berenguer-Murcia, Gilber Vela-Gutierrez, Jamal S. M. Sabir, Veymar G. Tacias-Pascacio, Roberto Fernandez-Lafuente
Summary: This article highlights the complexities of immobilizing pepsin and discusses potential methods and applications. The lack of primary amino groups on the enzyme's surface and its poor stability at alkaline pH values present challenges for immobilization. The review also proposes possibilities for achieving both covalent immobilization and stabilization of pepsin through multipoint covalent attachment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Food Science & Technology
Caroline Carboni Martins, Rafael C. Rodrigues, Giovana Domeneghini Mercali, Eliseu Rodrigues
Summary: Most studies on phenolic compounds (PC) have focused on extractable phenolic compounds (EPC), while non-extractable phenolic compounds (NEPC) play an important role in food matrices. To understand the health effects of NEPC intake, it is crucial to identify the types and content of NEPC in foods. This review evaluated 182 studies in the past five years on NEPC in foods, highlighting critical points in the analysis. Exhaustive extraction of EPC should be performed before hydrolysis, aggressive hydrolysis methods should be optimized, and spectrophotometric methods should be avoided for quantification. Liquid chromatography coupled with a diode array detector is the most suitable technique, and identification can be assisted by bioinformatics.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Energy & Fuels
Jessica Chaparro-Garnica, Miriam Navlani-Garcia, David Salinas-Torres, Angel Berenguer-Murcia, Emilia Morallon, Diego Cazorla-Amoros
Summary: In this study, Pd-based biomass-derived carbon-supported catalysts were developed using a simple method. The effects of modifying the metal phase and support, as well as the synthetic protocol used, were assessed. It was found that the resulting PdAg-based catalysts supported on N-doped biomass-derived carbon materials exhibited suitable activity and excellent stability under reaction conditions.
Article
Biochemistry & Molecular Biology
Daniel Castaneda-Valbuena, Angel Berenguer-Murcia, Roberto Fernandez-Lafuente, Roberto Morellon-Sterling, Veymar G. Tacias-Pascacio
Summary: The fishing industry generates valuable waste that contains a high amount of proteins. Processing these proteins can lead to products with nutritional, pharmacological, and technological benefits due to the presence of peptides. This review compiles investigations on the production of peptides from fish proteins using pepsin as a catalyst, highlighting their biological properties and potential uses.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
Gustavo P. P. Costa, Leonardo B. B. Queiroz, Vitor Manfroi, Rafael C. C. Rodrigues, Plinho F. F. Hertz
Summary: The study aimed to develop a low-cost support using gelatin, a residue from capsules, to immobilize the ALDC enzyme. The results showed that the immobilized enzyme had high activity, stability, and reusability, and exhibited good adaptability to different temperatures and pH levels.
Article
Infectious Diseases
Jennifer Ruiz-Duran, Rodrigo Torres, Elena E. Stashenko, Claudia Ortiz
Summary: In this study, the antifungal and antibiofilm potential of fifty essential oils (EOs) on three Candida species were evaluated. The EOs from Lippia alba and L. origanoides exhibited the highest antifungal activity against C. auris and showed antifungal and antibiofilm activity against all three Candida species.
Article
Chemistry, Applied
Eli Emanuel Esparza-Flores, Larissa Bertoldo Siquiera, Fernanda Dias Cardoso, Tania Haas Costa, Edilson Valmir Benvenutti, Iliana E. Medina-Ramirez, Mercedes Perullini, Patricio R. Santagapita, Rafael C. Rodrigues, Plinho F. Hertz
Summary: A comprehensive structural characterization of the chitosan-genipin complex was conducted using various techniques, showing modifications in the porosity and microstructure of the chitosan-genipin beads. The results demonstrated that the complex exhibited plasticity and dynamic behavior, with changes in fractality in response to pH environment and the crosslinking process. The immobilized enzyme on the modified particles showed high stability in continuous production. Evaluation: 8/10.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
El-Hocine Siar, Roberto Morellon-Sterling, Diego Carballares, Javier Rocha-Martin, Oveimar Barbosa, Juan M. Bolivar, Roberto Fernandez-Lafuente
Summary: The immobilization of ficin extract on glyoxyl agarose beads stabilized the enzyme, but the stability was highest when immobilized for 3 hours. The biocatalyst prepared for 24 hours was less stable when thermally inactivated in the presence of air. Incubating the thermally inactivated biocatalysts with dithiothreitol allowed for the recovery of almost total activity, with better recovery observed for the 24 hour biocatalyst. This suggests that the faster enzyme inactivation of the 24 hour biocatalyst was due to Cys oxidation.
PROCESS BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Pamela M. da Silva, Eli Emanuel Esparza-Flores, Anike H. Virgili, Eliana W. de Menezes, Roberto Fernandez-Lafuente, Lucas Dal Magro, Rafael C. Rodrigues
Summary: This study investigated the impact of different support matrices and active groups on the nutritional properties of orange juice after clarification. The results showed that immobilized enzyme on chitosan activated using genipin exhibited the highest catalytic activity and effectively reduced the turbidity of orange juice. The enzymatic treatment also maintained the natural sugar content, total soluble solids, and pH of the juice but caused color changes.
Article
Biotechnology & Applied Microbiology
Denise W. de Melo, Roberto Fernandez-Lafuente, Rafael C. Rodrigues
Summary: This study aimed to improve the application of l-asparaginase in reducing acrylamide formation in food and treating cancer by immobilizing it in different supports. The results showed that immobilization in amino-epoxy-agarose offered the highest immobilization yield (76%), immobilization efficiency (89.8%), and relative activity (68.2%). The immobilized l-asparaginase showed improved catalytic efficiency and stability, preserving more than 70% of activity after 7 cycles of reuse and maintaining about 82% of activity after 60 days of storage at 4°C.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Jose Renato Guimaraes, Kaique Souza Goncalves Cordeiro Oliveira, Maria Carolina Pereira Goncalves, Joao Paulo Romanelli, Laiane Antunes Lopes, Angel Berenguer-Murcia, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli
Summary: This article reviews the research on the immobilization of lipases on hydrophobic supports using systematic mapping (SM) concepts. It covers a wide range of lipases and supports, discussing their advantages and highlighting issues related to the immobilization process. Strategies to overcome these issues, such as immobilization on heterofunctional supports or intermolecular crosslinking, are also discussed. The structure, distribution, and co-occurrence frequency of lipases and supports are analyzed to determine potential research hotspots and unexplored advances in knowledge.
REACTION CHEMISTRY & ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Jose Renato Guimaraes, Kaique Souza Goncalves Cordeiro Oliveira, Maria Carolina Pereira Goncalves, Joao Paulo Romanelli, Laiane Antunes Lopes, Angel Berenguer-Murcia, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli
Summary: This article presents a systematic review of literature on the immobilization of lipases on hydrophobic supports. The study identifies various lipases and supports used in the research, discusses the advantages of immobilization on hydrophobic supports, and highlights problems and strategies for overcoming them.
REACTION CHEMISTRY & ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Mario Garcia-Rodriguez, Gabriel Alemany-Molina, Lorena Maria Sanchez-Moreno, Jessica Alejandra Chaparro-Garnica, Javier Fernandez-Catala, Laura Cano-Casanova, Angel Berenguer-Murcia, David Salinas-Torres, Miriam Navlani-Garcia
BOLETIN DEL GRUPO ESPANOL DEL CARBON
(2022)