Development of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms

Total HIV DNA: a global marker of HIV persistence

(2018) Christine Rouzioux et al.   Retrovirology

Digital PCR as a tool to measure HIV persistence

(2018) Sofie Rutsaert et al.   Retrovirology

Proviral Latency, Persistent Human Immunodeficiency Virus Infection, and the Development of Latency Reversing Agents

(2017) David M. Margolis et al.   JOURNAL OF INFECTIOUS DISEASES

Evaluation of the immunogenicity and impact on the latent HIV-1 reservoir of a conserved region vaccine, MVA.HIVconsv, in antiretroviral therapy-treated subjects

(2017) Gemma Hancock et al.   Journal of the International AIDS Society

Identification of Genetically Intact HIV-1 Proviruses in Specific CD4 + T Cells from Effectively Treated Participants

(2017) Bonnie Hiener et al.   Cell Reports

Extensive virologic and immunologic characterization in an HIV-infected individual following allogeneic stem cell transplant and analytic cessation of antiretroviral therapy: A case study

(2017) Nathan W. Cummins et al.   PLOS MEDICINE

Early Antiretroviral Therapy Is Associated with Lower HIV DNA Molecular Diversity and Lower Inflammation in Cerebrospinal Fluid but Does Not Prevent the Establishment of Compartmentalized HIV DNA Populations

(2017) Michelli F. Oliveira et al.   PLoS Pathogens

High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay

(2017) Robert W. Yucha et al.   EBioMedicine

Decay of HIV DNA in the Reservoir and the Impact of Short Treatment Interruption in Kenyan Infants

(2017) Mark D Pankau et al.   Open Forum Infectious Diseases

Virological and immunological outcome of treatment interruption in HIV-1-infected subjects vaccinated with MVA-B

(2017) Miriam Rosás-Umbert et al.   PLoS One

Virological responses to lamivudine or emtricitabine when combined with tenofovir and a protease inhibitor in treatment-naïve HIV-1-infected patients in the Dutch AIDS Therapy Evaluation in the Netherlands (ATHENA) cohort

(2016) C Rokx et al.   HIV MEDICINE

The Future of Digital Polymerase Chain Reaction in Virology

(2016) Matthijs Vynck et al.   Molecular Diagnosis & Therapy

International AIDS Society global scientific strategy: towards an HIV cure 2016

(2016) Steven G Deeks et al.   NATURE MEDICINE

Defective proviruses rapidly accumulate during acute HIV-1 infection

(2016) Katherine M Bruner et al.   NATURE MEDICINE

Measuring Digital PCR Quality: Performance Parameters and Their Optimization

(2016) A. Lievens et al.   PLoS One

Ultra–sensitive droplet digital PCR for detecting a low–prevalence somatic GNAQ mutation in Sturge–Weber syndrome

(2016) Yuri Uchiyama et al.   Scientific Reports

Integrated and Total HIV-1 DNA Predict Ex Vivo Viral Outgrowth

(2016) Maja Kiselinova et al.   PLoS Pathogens

Evaluating Clonal Expansion of HIV-Infected Cells: Optimization of PCR Strategies to Predict Clonality

(2016) Sarah B. Laskey et al.   PLoS Pathogens

Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems

(2016) Julia K. Bialek et al.   PLoS One

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials

(2015) Philippe Corbisier et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Digital PCR for direct quantification of viruses without DNA extraction

(2015) Jernej Pavšič et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ddpcRquant: threshold determination for single channel droplet digital PCR experiments

(2015) Wim Trypsteen et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Establishment and Replenishment of the Viral Reservoir in Perinatally HIV-1-infected Children Initiating Very Early Antiretroviral Therapy

(2015) Marta Martínez-Bonet et al.   CLINICAL INFECTIOUS DISEASES

A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples

(2015) Coralie Coudray-Meunier et al.   INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

Human Immunodeficiency Virus Type 1 Monoclonal Antibodies Suppress Acute Simian-Human Immunodeficiency Virus Viremia and Limit Seeding of Cell-Associated Viral Reservoirs

(2015) Diane L. Bolton et al.   JOURNAL OF VIROLOGY

Towards an HIV-1 cure: measuring the latent reservoir

(2015) Katherine M. Bruner et al.   TRENDS IN MICROBIOLOGY

Comparison of droplet digital PCR and conventional quantitative PCR for measuring EGFR gene mutation

(2015) BO ZHANG et al.   Experimental and Therapeutic Medicine

Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir

(2015) K. J. Bosman et al.   Scientific Reports

The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo

(2015) Ole S. Søgaard et al.   PLoS Pathogens

During Stably Suppressive Antiretroviral Therapy Integrated HIV-1 DNA Load in Peripheral Blood is Associated with the Frequency of CD8 Cells Expressing HLA-DR/DP/DQ

(2015) Alessandra Ruggiero et al.   EBioMedicine

A Novel Assay to Measure the Magnitude of the Inducible Viral Reservoir in HIV-infected Individuals

(2015) Francesco Andrea Procopio et al.   EBioMedicine

Optimising droplet digital PCR analysis approaches for detection and quantification of bacteria: a case study of fire blight and potato brown rot

(2014) Tanja Dreo et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Antiretroviral-Free HIV-1 Remission and Viral Rebound After Allogeneic Stem Cell Transplantation

(2014) Timothy J. Henrich et al.   ANNALS OF INTERNAL MEDICINE

Broadly Neutralizing Antibodies and Viral Inducers Decrease Rebound from HIV-1 Latent Reservoirs in Humanized Mice

(2014) Ariel Halper-Stromberg et al.   CELL

Identification of Chromosomally Integrated Human Herpesvirus 6 by Droplet Digital PCR

(2014) R. H. Sedlak et al.   CLINICAL CHEMISTRY

Quantification of Integrated HIV DNA by Repetitive-Sampling Alu-HIV PCR on the Basis of Poisson Statistics

(2014) W. De Spiegelaere et al.   CLINICAL CHEMISTRY

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

(2014) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

(2014) Eva Malatinkova et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Improved Single-Copy Assays for Quantification of Persistent HIV-1 Viremia in Patients on Suppressive Antiretroviral Therapy

(2014) A. R. Cillo et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Gene Editing of CCR5 in Autologous CD4 T Cells of Persons Infected with HIV

(2014) Pablo Tebas et al.   NEW ENGLAND JOURNAL OF MEDICINE

Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA

(2014) Maja Kiselinova et al.   PLoS One

A Real Time PCR Platform for the Simultaneous Quantification of Total and Extrachromosomal HIV DNA Forms in Blood of HIV-1 Infected Patients

(2014) Anna Casabianca et al.   PLoS One

Broadly neutralizing antibodies suppress HIV in the persistent viral reservoir

(2014) T.-W. Chun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reliable reconstruction of HIV-1 whole genome haplotypes reveals clonal interference and genetic hitchhiking among immune escape variants

(2014) Aridaman Pandit et al.   Retrovirology

Validation of an ultrasensitive digital droplet PCR assay for HIV-2 plasma RNA quantification

(2014) Jean Ruelle et al.   Journal of the International AIDS Society

Activation of HIV Transcription with Short-Course Vorinostat in HIV-Infected Patients on Suppressive Antiretroviral Therapy

(2014) Julian H. Elliott et al.   PLoS Pathogens

Panobinostat, a histone deacetylase inhibitor, for latent-virus reactivation in HIV-infected patients on suppressive antiretroviral therapy: a phase 1/2, single group, clinical trial

(2014) Thomas A Rasmussen et al.   Lancet HIV

Replication-Competent Noninduced Proviruses in the Latent Reservoir Increase Barrier to HIV-1 Cure

(2013) Ya-Chi Ho et al.   CELL

Dynamics of Cellular HIV-1 DNA Levels Over 144 Weeks of Darunavir/Ritonavir Monotherapy Versus Triple Therapy in the MONET Trial

(2013) Anna Maria Geretti et al.   HIV CLINICAL TRIALS

Plasma Viremia and Cellular HIV-1 DNA Persist Despite Autologous Hematopoietic Stem Cell Transplantation for HIV-Related Lymphoma

(2013) Anthony R. Cillo et al.   JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES

Development and Evaluation of an Affordable Real-Time Qualitative Assay for Determining HIV-1 Virological Failure in Plasma and Dried Blood Spots

(2013) S. C. Aitken et al.   JOURNAL OF CLINICAL MICROBIOLOGY

The Distribution of HIV DNA and RNA in Cell Subsets Differs in Gut and Blood of HIV-Positive Patients on ART: Implications for Viral Persistence

(2013) Steven A. Yukl et al.   JOURNAL OF INFECTIOUS DISEASES

The effect of multiple primer–template mismatches on quantitative PCR accuracy and development of a multi-primer set assay for accurate quantification of pcrA gene sequence variants

(2013) Brett M. Ledeker et al.   JOURNAL OF MICROBIOLOGICAL METHODS

HIV Populations Are Large and Accumulate High Genetic Diversity in a Nonlinear Fashion

(2013) F. Maldarelli et al.   JOURNAL OF VIROLOGY

Absence of Detectable HIV-1 Viremia after Treatment Cessation in an Infant

(2013) Deborah Persaud et al.   NEW ENGLAND JOURNAL OF MEDICINE

Highly Precise Measurement of HIV DNA by Droplet Digital PCR

(2013) Matthew C. Strain et al.   PLoS One

Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR

(2012) Timothy J. Henrich et al.   JOURNAL OF VIROLOGICAL METHODS

Global trends in molecular epidemiology of HIV-1 during 2000–2007

(2011) Joris Hemelaar et al.   AIDS

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

(2011) Leonardo B. Pinheiro et al.   ANALYTICAL CHEMISTRY

Cellular HIV-1 DNA quantification and short-term and long-term response to antiretroviral therapy

(2011) B. Masquelier et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Steady increase in cellular HIV-1 load during the asymptomatic phase of untreated infection despite stable plasma viremia

(2010) Alexander O Pasternak et al.   AIDS

Rational design of HIV-1 fluorescent hydrolysis probes considering phylogenetic variation and probe performance

(2010) Claudia F. Althaus et al.   JOURNAL OF VIROLOGICAL METHODS

HIV-1 replication and immune dynamics are affected by raltegravir intensification of HAART-suppressed subjects

(2010) Maria J Buzón et al.   NATURE MEDICINE

Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

(2009) Brian Boyle et al.   BMC BIOTECHNOLOGY

The Effect of Primer-Template Mismatches on the Detection and Quantification of Nucleic Acids Using the 5′ Nuclease Assay

(2009) Ralph Stadhouders et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation

(2009) Nicolas Chomont et al.   NATURE MEDICINE

Long-Term Control of HIV byCCR5Delta32/Delta32 Stem-Cell Transplantation

(2009) Gero Hütter et al.   NEW ENGLAND JOURNAL OF MEDICINE

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

(2008) D. Bru et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Highly Sensitive Methods Based on Seminested Real-Time Reverse Transcription-PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA

(2008) A. O. Pasternak et al.   JOURNAL OF CLINICAL MICROBIOLOGY

LTR real-time PCR for HIV-1 DNA quantitation in blood cells for early diagnosis in infants born to seropositive mothers treated in HAART area (ANRS CO 01)

(2008) Avettand-Fènoël Véronique et al.   JOURNAL OF MEDICAL VIROLOGY

Human Immunodeficiency Virus Type 1 Population Genetics and Adaptation in Newly Infected Individuals

(2008) M. Kearney et al.   JOURNAL OF VIROLOGY