The Future of Digital Polymerase Chain Reaction in Virology

Quantitative Assessment of Commutability for Clinical Viral Load Testing Using a Digital PCR-Based Reference Standard

(2016) L. Tang et al.   JOURNAL OF CLINICAL MICROBIOLOGY

A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System

(2016) Coralie Coudray-Meunier et al.   PLoS One

Measuring Digital PCR Quality: Performance Parameters and Their Optimization

(2016) A. Lievens et al.   PLoS One

Integrated and Total HIV-1 DNA Predict Ex Vivo Viral Outgrowth

(2016) Maja Kiselinova et al.   PLoS Pathogens

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials

(2015) Philippe Corbisier et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Digital PCR for direct quantification of viruses without DNA extraction

(2015) Jernej Pavšič et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ddpcRquant: threshold determination for single channel droplet digital PCR experiments

(2015) Wim Trypsteen et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Robust regression methods for real-time polymerase chain reaction

(2015) Wim Trypsteen et al.   ANALYTICAL BIOCHEMISTRY

Statistical Models for the Analysis and Design of Digital Polymerase Chain Reaction (dPCR) Experiments

(2015) Robert M. Dorazio et al.   ANALYTICAL CHEMISTRY

Rational Design of Digital Assays

(2015) Pawel R. Debski et al.   ANALYTICAL CHEMISTRY

Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR

(2015) Alison S. Devonshire et al.   ANALYTICAL CHEMISTRY

A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system

(2015) Di Mu et al.   BIOTECHNOLOGY LETTERS

MethyLight droplet digital PCR for detection and absolute quantification of infrequently methylated alleles

(2015) Ming Yu et al.   Epigenetics

A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples

(2015) Coralie Coudray-Meunier et al.   INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

Comparative Evaluation of Three Commercial Quantitative Cytomegalovirus Standards by Use of Digital and Real-Time PCR

(2015) R. T. Hayden et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples

(2015) Alexandra S. Whale et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations

(2015) Sean C. Taylor et al.   JOURNAL OF VIROLOGICAL METHODS

Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir

(2015) K. J. Bosman et al.   Scientific Reports

Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material

(2015) Lianhua Dong et al.   Scientific Reports

Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based Assays

(2015) Giada Mattiuzzo et al.   PLoS One

Optimising droplet digital PCR analysis approaches for detection and quantification of bacteria: a case study of fire blight and potato brown rot

(2014) Tanja Dreo et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

(2014) Gavin Nixon et al.   ANALYTICAL CHEMISTRY

Impact of variance components on reliability of absolute quantification using digital PCR

(2014) Bart KM Jacobs et al.   BMC BIOINFORMATICS

Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool

(2014) J. F. Huggett et al.   CLINICAL CHEMISTRY

Identification of Chromosomally Integrated Human Herpesvirus 6 by Droplet Digital PCR

(2014) R. H. Sedlak et al.   CLINICAL CHEMISTRY

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

(2014) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Antiretrovirals for HIV prevention: when should they be recommended?

(2014) Maja Kiselinova et al.   Expert Review of Anti-Infective Therapy

The potential advantages of digital PCR for clinical virology diagnostics

(2014) Ruth Hall Sedlak et al.   EXPERT REVIEW OF MOLECULAR DIAGNOSTICS

Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples

(2014) Rongchang Yang et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses

(2014) Jernej Pavšič et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

(2014) Eva Malatinkova et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus

(2014) R. H. Sedlak et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Digital droplet PCR (ddPCR) for the precise quantification of human T-lymphotropic virus 1 proviral loads in peripheral blood and cerebrospinal fluid of HAM/TSP patients and identification of viral mutations

(2014) Giovanna S. Brunetto et al.   JOURNAL OF NEUROVIROLOGY

Low copy target detection by Droplet Digital PCR through application of a novel open access bioinformatic pipeline, ‘definetherain’

(2014) Mathew Jones et al.   JOURNAL OF VIROLOGICAL METHODS

High-throughput and sensitive next-generation droplet digital PCR assay for the quantitation of the hepatitis C virus mutation at core amino acid 70

(2014) Motokazu Mukaide et al.   JOURNAL OF VIROLOGICAL METHODS

Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples

(2014) Nejc Rački et al.   Plant Methods

Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA

(2014) Maja Kiselinova et al.   PLoS One

Quantitative detection of human enteric adenoviruses in river water by microfluidic digital polymerase chain reaction

(2014) Naohiro Kishida et al.   WATER SCIENCE AND TECHNOLOGY

One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses

(2013) Nejc Rački et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

Tolerance of Droplet-Digital PCR vs Real-Time Quantitative PCR to Inhibitory Substances

(2013) T. C. Dingle et al.   CLINICAL CHEMISTRY

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

(2013) Ross J. Haynes et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Absolute quantification by droplet digital PCR versus analog real-time PCR

(2013) Christopher M Hindson et al.   NATURE METHODS

Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR

(2013) Dany Morisset et al.   PLoS One

Highly Precise Measurement of HIV DNA by Droplet Digital PCR

(2013) Matthew C. Strain et al.   PLoS One

Evaluation of Digital PCR for Absolute RNA Quantification

(2013) Rebecca Sanders et al.   PLoS One

Viral diagnostics in the era of digital polymerase chain reaction

(2012) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Digital PCR for Noninvasive Detection of Aneuploidy: Power Analysis Equations for Feasibility

(2012) Mark I. Evans et al.   FETAL DIAGNOSIS AND THERAPY

Comparison of Droplet Digital PCR to Real-Time PCR for Quantitative Detection of Cytomegalovirus

(2012) R. T. Hayden et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR

(2012) Timothy J. Henrich et al.   JOURNAL OF VIROLOGICAL METHODS

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

(2012) Alexandra S. Whale et al.   NUCLEIC ACIDS RESEARCH

Quantitative Analysis of Viral Load per Haploid Genome Revealed the Different Biological Features of Merkel Cell Polyomavirus Infection in Skin Tumor

(2012) Satoshi Ota et al.   PLoS One

Molecular quantification of environmental DNA using microfluidics and digital PCR

(2012) Tatsuhiko Hoshino et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

(2011) Leonardo B. Pinheiro et al.   ANALYTICAL CHEMISTRY

Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR

(2011) Jason E. Kreutz et al.   ANALYTICAL CHEMISTRY

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number

(2011) Benjamin J. Hindson et al.   ANALYTICAL CHEMISTRY

Digital PCR provides absolute quantitation of viral load for an occult RNA virus

(2011) Richard Allen White et al.   JOURNAL OF VIROLOGICAL METHODS

Quantitative and sensitive detection of rare mutations using droplet-based microfluidics

(2011) Deniz Pekin et al.   LAB ON A CHIP

Quantifying EGFR Alterations in the Lung Cancer Genome with Nanofluidic Digital PCR Arrays

(2010) J. Wang et al.   CLINICAL CHEMISTRY

Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number

(2009) Somanath Bhat et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Elimination of amplification artifacts in real-time reverse transcription PCR using laser capture microdissected samples

(2008) Ward De Spiegelaere et al.   ANALYTICAL BIOCHEMISTRY

Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device

(2008) Simant Dube et al.   PLoS One