Oral gavage is thought to be stressful for mice. This article indicates no major differences in cortisol levels of control vs study mice (male CD1's) in the first week but cortisol levels increased. There is added risk of perforating the esophagus so not my favorite method of dosing mice.
Well written protocol. It covers all aspects of rodent surgery preparation for the new surgeon including the most important one which is aseptic techniques.
Dexamethasone, traditionally used to relieve inflammation and treat arthritis, is such a versatile drug. Lately, it has been studied in PTSD amongst war veterans by Mount Sinai researchers and even as potential COVID treatment during the current pandemic.
They passaged the aggressive metastatic human small-cell lung cancer cell line in mice. This is quite common practice now to develop new in vivo cancer models.
Klotho mice have the 'klotho' gene which is involved in the suppression of different ageing phenotypes and transgenic mice that over express the klotho gene live longer than the wild type mice.
Apparently the engineered T cells are programmed to detect CD5 on the T cells and they selectively kill malignant T cells only whereas in normal T cells the cytotoxic pathway is blocked by PI-9 and Bcl-2.
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