4.7 Article

Preparative separation of triterpene alcohol ferulates from rice bran oil using a high performance counter-current chromatography

Journal

FOOD CHEMISTRY
Volume 139, Issue 1-4, Pages 919-924

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.01.106

Keywords

HPCCC; Rice bran oil; Triterpene alcohol ferulate

Funding

  1. SJTU 985-III disciplines platform and talent funds [TS0414115001, TS0320215001]
  2. Special Fund for Agro-scientific Research in the Public Interest [201203069]
  3. Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.

Ask authors/readers for more resources

A novel method for the separation of two major triterpene alcohol ferulates from rice bran oil (RBO) was developed using a high performance counter-current chromatography (HPCCC). A two-phase solvent system of n-hexane-acetonitrile (1:1, v/v) was applied to purify cycloartenyl ferulate (CAF) and 24-methylene cycloartanyl ferulate (24-mCAF) from RBO. The yields were 20.50 +/- 2.60 mg CAF and 12.62 +/- 1.15 mg 24-mCAF from 390 mg RBO through a two-step separation procedure. The purities of the two compounds were 97.97 +/- 0.90% and 95.50 +/- 0.75%, respectively, as determined by high performance liquid chromatography (HPLC). Their chemical structures were confirmed by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and H-1, C-13 and 2D nuclear magnetic resonance (NMR). This represents the first report on direct separation of CAF and 24-mCAF from RBO by HPCCC. (C) 2013 Elsevier Ltd. All rights reserved.

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