Hydrophobic supplements in cell-free systems: Designing artificial environments for membrane proteins

Surfactant-free purification of membrane proteins with intact native membrane environment

(2015) Mohammed Jamshad et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments

(2013) Davide Proverbio et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins

(2013) Franz Hagn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystal Structure of MraY, an Essential Membrane Enzyme for Bacterial Cell Wall Synthesis

(2013) B. C. Chung et al.   SCIENCE

Cell-free synthesis of cytochrome bo3 ubiquinol oxidase in artificial membranes

(2012) Ahu Arslan Yildiz et al.   ANALYTICAL BIOCHEMISTRY

Detergent-Free Formation and Physicochemical Characterization of Nanosized Lipid-Polymer Complexes: Lipodisq

(2012) Marcella C. Orwick et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Nonionic Homopolymeric Amphipols: Application to Membrane Protein Folding, Cell-Free Synthesis, and Solution Nuclear Magnetic Resonance

(2012) Paola Bazzacco et al.   BIOCHEMISTRY

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase

(2012) Christian Roos et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

(2012) Agalya Periasamy et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Modified lipid and protein dynamics in nanodiscs

(2012) Karsten Mörs et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

(2012) Christian Roos et al.   MOLECULAR MEMBRANE BIOLOGY

The Cell-Free Integration of a Polytopic Mitochondrial Membrane Protein into Liposomes Occurs Cotranslationally and in a Lipid-Dependent Manner

(2012) Ashley R. Long et al.   PLoS One

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

(2011) Michael Zocher et al.   ACS Nano

Solution NMR Structure of Proteorhodopsin

(2011) Sina Reckel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Amphipols From A to Z*

(2011) J.-L. Popot et al.   Annual Review of Biophysics

Refolding of proteins from inclusion bodies: rational design and recipes

(2011) Anindya Basu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Nanodiscs versus Macrodiscs for NMR of Membrane Proteins

(2011) Sang Ho Park et al.   BIOCHEMISTRY

Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant

(2011) Iulia Blesneac et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes

(2011) E.N. Lyukmanova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs

(2011) Jian-Ping Yang et al.   BMC BIOTECHNOLOGY

Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

(2011) Akira Nozawa et al.   BMC BIOTECHNOLOGY

Functional Expression of the PorAH Channel fromCorynebacterium glutamicumin Cell-free Expression Systems

(2011) Parthasarathi Rath et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes

(2011) Yi Ma et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Consequences of the Overexpression of a Eukaryotic Membrane Protein, the Human KDEL Receptor, in Escherichia coli

(2011) Mirjam M. Klepsch et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal Structure of the Eukaryotic Light-Driven Proton-Pumping Rhodopsin, Acetabularia Rhodopsin II, from Marine Alga

(2011) Takashi Wada et al.   JOURNAL OF MOLECULAR BIOLOGY

Cell-Free Expression and Assembly of ATP Synthase

(2011) Doreen Matthies et al.   JOURNAL OF MOLECULAR BIOLOGY

Green Proteorhodopsin Reconstituted into Nanoscale Phospholipid Bilayers (Nanodiscs) as Photoactive Monomers

(2011) Matthew J. Ranaghan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystallizing membrane proteins using lipidic bicelles

(2011) Rachna Ujwal et al.   METHODS

Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems

(2011) Karolina Corin et al.   PLoS One

Peptide surfactants for cell-free production of functional G protein-coupled receptors

(2011) X. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents

(2011) Christian Klammt et al.   PROTEIN SCIENCE

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunitafold similar to the protein in the cell membrane

(2011) Eva-Maria E. Uhlemann et al.   PROTEIN SCIENCE

New advances in production and functional folding of G-protein-coupled receptors

(2011) Jean-Louis Banères et al.   TRENDS IN BIOTECHNOLOGY

Amphipols, Nanodiscs, and Fluorinated Surfactants: Three Nonconventional Approaches to Studying Membrane Proteins in Aqueous Solutions

(2010) Jean-Luc Popot   Annual Review of Biochemistry

Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents

(2010) Takahisa Genji et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL

(2010) Catherine Berrier et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Non-reducing trisaccharide fatty acid monoesters: Novel detergents in membrane biochemistry

(2010) Ignacio Pérez-Victoria et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation

(2010) Yuki Moritani et al.   FEBS Journal

Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor

(2010) Friederike Junge et al.   JOURNAL OF STRUCTURAL BIOLOGY

Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

(2010) Pil Seok Chae et al.   NATURE METHODS

In the cauldron of cell-free synthesis of membrane proteins: playing with new surfactants

(2010) Kyu-Ho Park et al.   New Biotechnology

Structural investigation of the C-terminal catalytic fragment of presenilin 1

(2010) S. Sobhanifar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis

(2010) I. Maslennikov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Amphipol-Assisted in Vitro Folding of G Protein-Coupled Receptors

(2009) Tassadite Dahmane et al.   BIOCHEMISTRY

Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes

(2009) Makoto Kaneda et al.   BIOMATERIALS

Micellar and Biochemical Properties of (Hemi)Fluorinated Surfactants Are Controlled by the Size of the Polar Head

(2009) Cécile Breyton et al.   BIOPHYSICAL JOURNAL

Cell-free synthesis of functional aquaporin Z in synthetic liposomes

(2009) Norman T. Hovijitra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Lipopeptide detergents for membrane protein studies

(2009) Gilbert G Privé   CURRENT OPINION IN STRUCTURAL BIOLOGY

Membrane Proteins Solubilized Intact in Lipid Containing Nanoparticles Bounded by Styrene Maleic Acid Copolymer

(2009) Timothy J. Knowles et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Functional in vitro assembly of the integral membrane bacterial thermosensor DesK

(2009) Mariana Martín et al.   PROTEIN EXPRESSION AND PURIFICATION

Single-step production of functional OEP24 proteoliposomes

(2009) Lavinia Liguori et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of functional bacteriorhodopsin by anEscherichia colicell-free protein synthesis system supplemented with steroid detergent and lipid

(2009) Kazumi Shimono et al.   PROTEIN SCIENCE

Revolutionizing membrane protein overexpression in bacteria

(2009) Susan Schlegel et al.   Microbial Biotechnology

Cell Free Expression and Functional Reconstitution of Eukaryotic Drug Transporters†

(2008) Thorsten Keller et al.   BIOCHEMISTRY

High yield cell-free production of integral membrane proteins without refolding or detergents

(2008) Jessica J. Wuu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins

(2008) Lavinia Liguori et al.   JOURNAL OF CONTROLLED RELEASE

In Vitro Multimerization and Membrane Insertion of Bacterial Outer Membrane Secretin PulD

(2008) Ingrid Guilvout et al.   JOURNAL OF MOLECULAR BIOLOGY

Designer Peptide Surfactants Stabilize Functional Photosystem-I Membrane Complex in Aqueous Solution for Extended Time⊥

(2008) Kazuya Matsumoto et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach

(2008) Federico Katzen et al.   JOURNAL OF PROTEOME RESEARCH

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles

(2008) Jenny A. Cappuccio et al.   MOLECULAR & CELLULAR PROTEOMICS

Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

(2008) L. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis

(2007) Shin-ichiro M. Nomura et al.   JOURNAL OF BIOTECHNOLOGY

Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton pump

(2007) Pontus Gourdon et al.   PROTEIN EXPRESSION AND PURIFICATION