Article
Polymer Science
Masafumi Yamato, Ayano Imai, Hiroyoshi Kawakami
Summary: This study investigated the thermal behavior of a polymer with intrinsic microporosity (PIM-1) membrane using thermomechanical analysis and differential scanning calorimetry. It was found that the coefficient of thermal expansion (CTE) remained constant below 110 degrees C, with inflection points observed between 120-150 degrees C. The CTE after the inflection point was significantly influenced by the fabrication conditions and thermal history. Cooperative molecular motion was observed below 200 degrees C.
Article
Chemistry, Physical
Riccardo Carloni, Maria Francesca Ottaviani, Mario Ficker, Jorn B. Christensen
Summary: The study analyzed the interactions between differently functionalized PAMAM dendrimers and model membranes. The results showed that acetamide- and pyrrolidone-dendrimers can be used as good alternatives to amino-dendrimers for drug delivery.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Gustavo Penteado Battesini Carretero, Greice Kelle Viegas Saraiva, Magali Aparecida Rodrigues, Sumika Kiyota, Marcelo Porto Bemquerer, Hernan Chaimovich, Iolanda Midea Cuccovia
Summary: Antimicrobial peptide BP100 demonstrates antibacterial activity, low toxicity, and increased membrane permeability. The fluorescent BP100 analogue NAPHT-BP100 interacts with cell membranes and DNA, showing stronger antibacterial and hemolytic activities compared to BP100, potentially making it an efficient antimicrobial agent for dermatological use.
Article
Polymer Science
Sibele Santos Fernandes, Leonardo Greque, Maria de Fatima C. Santos, Leice M. R. de Novais, Caroline D. R. M. D'Oca, Carlos Prentice, Myriam de las M. Salas-Mellado
Summary: Spray drying is an effective strategy to minimize the oxidation of chia oil, and the assessment showed that the microparticles have high yield, encapsulation efficiency, and a small particle size range. Furthermore, thermal evaluation and storage indicated an increase in stability of the microparticles.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Arianna Actis, Michele Melchionna, Giacomo Filippini, Paolo Fornasiero, Maurizio Prato, Enrico Salvadori, Mario Chiesa
Summary: This study assesses the influence of morphology and light-excitation on the number and chemical nature of radical defects in carbon nitride (CN) and derives the spatial distribution of native radicals in CN using magnetic dipole-dipole coupling.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Poornima Budime Santhosh, Julia Genova, Zdravka Slavkova, Hassan Chamati
Summary: Melatonin plays a crucial role in regulating sleep-wake cycles and has shown protective effects against cancer and neurodegenerative disorders. This study examines the effects of melatonin on the thermal and structural properties of SOPC phospholipid membranes. The results indicate that melatonin interacts with the membrane lipids, reducing phase transition temperature and increasing membrane fluidity.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Physical
Yubexi Correa, Sarah Waldie, Michel Thepaut, Samantha Micciulla, Martine Moulin, Franck Fieschi, Harald Pichler, V. Trevor Forsyth, Michael Haertlein, Marite Cardenas
Summary: Cholesterol levels and lipid disorders are correlated with Covid-19 severity. The SARS-CoV-2 spike (S) protein interferes with lipoprotein function, particularly dependent on cholesterol.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Ekaterina Kostyurina, Juergen Allgaier, Margarita Kruteva, Henrich Frielinghaus, Agnes Csiszar, Stephan Foerster, Ralf Biehl
Summary: The translocation of macromolecules across cell membranes is crucial for cells and drug delivery. This study investigates the factors influencing membrane partition and translocation rate, and reveals that membrane insertion is a key step determining the translocation rate. Furthermore, it demonstrates that macromolecules with different monomer structures can have similar translocation rates, providing insights for optimizing translocation conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Inorganic & Nuclear
Nitish Kumar Garg, Yogesh Goriya, Seetharaman Manojveer, Senada Muratovic, Damir Pajic, Mario Cetina, Irina Petreska, Yulia Krupskaya, Vladislav Kataev, Magnus T. Johnson, Ola F. Wendt, Dijana Zilic
Summary: A new mononuclear iron(iii) octahedral complex, [FeL6](BF4)3, has been synthesized from the reaction between 2-hydroxy-6-methylpyridine (L) and iron(ii) tetrafluoroborate. The color of the complex changes reversibly from red to yellow-orange with temperature. Magnetization measurements show that the iron(iii) in [FeL6](BF4)3 is in a high-spin state. Although spin-crossover is not observed, X-band and multifrequency high-field/high-frequency electron spin resonance (ESR) spectroscopy reveal unusual iron(iii) spectra at room temperature and changes with cooling.
DALTON TRANSACTIONS
(2022)
Article
Multidisciplinary Sciences
Peipei Zuo, Chunchun Ye, Zhongren Jiao, Jian Luo, Junkai Fang, Ulrich S. Schubert, Neil B. McKeown, T. Leo Liu, Zhengjin Yang, Tongwen Xu
Summary: The development of efficient ion-transport membranes is crucial for improving separation processes and electrochemical technologies. We have designed synthetic membranes with covalently bonded polymer frameworks that achieve near-frictionless ion flow through rigidly confined ion channels. These membranes have the potential to greatly enhance the performance of electrochemical devices and molecular separation.
Article
Chemistry, Multidisciplinary
Xuxiao Liu, Ruilian Qi, Shumu Li, Wuran Liu, Yueyang Yu, Jihui Wang, Songmei Wu, Kejian Ding, Yu Yu
Summary: Covalent organic frameworks (COFs) with porphyrins as structural units are a new type of porous organic polymers that exhibit regular structure, abundant porosity, and good stability. A new COF structure, TA-Por-sp(2)-COF, was constructed by linking porphyrin with the triazine ring through the self-polymerization of a cyano group-terminated porphyrin. The resulting highly ordered two-dimensional topological structure exhibited high photocatalytic performance in organic reactions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Polymer Science
Charlotte Frost, Sandra Wiedbrauk, Elizabeth Graham, Nageshwar R. Yepuri, Andrew R. J. Nelson, Nathan R. B. Boase
Summary: Amphipathic copolymers are synthesized and studied for their interactions with model lipid membranes using complementary techniques. The results show that these materials can interact with Langmuir lipid monolayers, and neutron reflectometry confirms the intercalation of polymers with the monolayers due to hydrophobic interactions. In situ quartz crystal microbalance (QCM) model is used to study more complex models of lipid bilayers, and it is found that the composition and hydrophobic comonomer affect the stability of these interactions, but have no effect on the viscoelasticity of the lipid membranes. This in-depth understanding of the interfacial interactions is crucial for further research on complex copolymers in nanomedicine, providing insights into important processes such as cellular entry and endosomal escape.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Yunfan Qiu, Asif Equbal, Chenjian Lin, Yuheng Huang, Paige J. Brown, Ryan M. Young, Matthew D. Krzyaniak, Michael R. Wasielewski
Summary: Photoexcited organic chromophores combined with stable radicals can be used as candidates for quantum information applications. In this study, the synthesis and characterization of 1,6,7,12-Tetra-(4-tert-butylphenoxy)-perylene-3,4 : 9,10-bis(dicarboximide) (tpPDI) linked to a partially deuterated alpha,gamma-bisdiphenylene-beta-phenylallyl radical (BDPA-d(16)) were performed using time-resolved optical and electron paramagnetic resonance (EPR) spectroscopies. The results showed efficient generation of a quartet state (Q) followed by a spin-polarized doublet ground state (D-0) upon photoexcitation of tpPDI-BDPA-d(16).
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Tomasz Czerniak, James P. Saenz
Summary: The presence of lipid membranes can affect the activity of RNA, with the interaction dependent on the nucleotide content, base pairing, and length of RNA. Short RNAs with guanine are crucial for the RNA-lipid interaction, and the formation of G-quadruplex further enhances lipid binding. By modifying the substrate sequence, a lipid-sensitive ribozyme reaction with riboswitch-like behavior can be generated.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Polymer Science
Jia-Ci Jhang, Chiao-Chi Cheng, Chen-Hung Huang, Yueh-Sheng Chen, Jia-Horng Lin, Ching-Wen Lou
Summary: Polylactic acid (PLA) possesses many advantages but its brittleness limits its application. This study modifies PLA by melt-blending it with Polystyrene-b-poly(ethylene-co-1-butene)-b-polystyrene (SEBS) and maleic anhydride grafted polypropylene (PP-g-MA) to produce antibacterial PLA/SEBS membranes.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Chemistry, Analytical
Franze Mueller, Andrea Graziadei, Juri Rappsilber
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Juan Francisco Bada Juarez, Andrew J. Harper, Peter J. Judge, Stephen R. Tonge, Anthony Watts
CHEMISTRY AND PHYSICS OF LIPIDS
(2019)
Article
Biochemistry & Molecular Biology
Luis Felipe S. Mendes, Mariana R. B. Batista, Peter J. Judge, Anthony Watts, Christina Redfield, Antonio J. Costa-Filho
Article
Biology
Andrea Graziadei, Frank Gabel, John Kirkpatrick, Teresa Carlomagno
Article
Multidisciplinary Sciences
Francis J. O'Reilly, Liang Xue, Andrea Graziadei, Ludwig Sinn, Swantje Lenz, Dimitry Tegunov, Cedric Bloetz, Neil Singh, Wim J. H. Hagen, Patrick Cramer, Jobrg Stuelke, Julia Mahamid, Juri Rappsilber
Article
Multidisciplinary Sciences
Juan F. Bada Juarez, Peter J. Judge, Suliman Adam, Danny Axford, Javier Vinals, James Birch, Tristan O. C. Kwan, Kin Kuan Hoi, Hsin-Yung Yen, Anthony Vial, Pierre-Emmanuel Milhiet, Carol V. Robinson, Igor Schapiro, Isabel Moraes, Anthony Watts
Summary: The study reveals significant differences between the ground and dark-adapted states of Archaerhodopsin-3 (AR3), with dynamics of water molecules and internal water networks playing key roles in this desensitization process.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Kin Kuan Hoi, Juan Francisco Bada Juarez, Peter J. Judge, Hsin-Yung Yen, Di Wu, Javier Vinals, Garrick F. Taylor, Anthony Watts, Carol Robinson
Summary: The study used native MS to investigate the spectra of membrane proteins in different membrane mimetics, revealing that SMA may favor selective solubilization of correctly folded proteins. The results provide insights into the correlation between PTMs, lipid interactions, and protein folding states.
Article
Microbiology
Sofia Banchenko, Ferdinand Krupp, Christine Gotthold, Joerg Buerger, Andrea Graziadei, Francis J. O'Reilly, Ludwig Sinn, Olga Ruda, Juri Rappsilber, Christian M. T. Spahn, Thorsten Mielke, Ian A. Taylor, David Schwefel
Summary: Viruses rely on host cell components for replication, with certain viral factors altering the specificity of host ubiquitin ligases to down-regulate antiviral factors. Studies have shown how viruses exploit the dynamic assembly of the CRL4(DCAF1) complex to optimize SAMHD1 ubiquitylation.
Review
Biochemistry & Molecular Biology
Andrea Graziadei, Juri Rappsilber
Summary: Crosslinking mass spectrometry is a versatile tool that provides structural insights into protein conformation and protein-protein interactions. It has been used for protein structure validation and integrative modeling, and complements the resolution revolution in electron microscopy. Its flexibility and high-throughput nature make it valuable in protein research.
Article
Biochemical Research Methods
Danny Axford, Peter J. Judge, Juan F. Bada Juarez, Tristan O. C. Kwan, James Birch, Javier Vinals, Anthony Watts, Isabel Moraes
Summary: Room-temperature diffraction methods are useful for dynamic studies of biological macromolecules. The researchers developed a sample-efficient environment using polymer films to study crystals grown in lipidic cubic phase. They successfully solved two structural states of the AR3 photoreceptor.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marcin Jaciuk, David Scherf, Karol Kaszuba, Monika Gaik, Alexander Rau, Anna Koscielniak, Roscislaw Krutyholowa, Michal Rawski, Paulina Indyka, Andrea Graziadei, Andrzej Chramiec-Glabik, Anna Biela, Dominika Dobosz, Ting-Yu Lin, Nour-el-Hana Abbassi, Alexander Hammermeister, Juri Rappsilber, Jan Kosinski, Raffael Schaffrath, Sebastian Glatt
Summary: The Elongator complex is responsible for modifying tRNAs in eukaryotes by adding a carboxymethyl group to wobble uridines. This modification is crucial for maintaining cellular proteome integrity and protecting against neurodegenerative diseases.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Vu Thuy Khanh Le-Trilling, Sofia Banchenko, Darius Paydar, Pia Madeleine Leipe, Lukas Binting, Simon Lauer, Andrea Graziadei, Robin Klingen, Christine Gotthold, Joerg Buerger, Thilo Bracht, Barbara Sitek, Robert Jan Lebbink, Anna Malyshkina, Thorsten Mielke, Juri Rappsilber, Christian M. T. Spahn, Sebastian Voigt, Mirko Trilling, David Schwefel
Summary: This study revealed that rat cytomegalovirus infection induces the loss of transcription factor STAT2, which is crucial for antiviral interferon signaling. It was found that viral protein E27 exploits the host-cell CRL4 complexes to degrade STAT2 through poly-ubiquitylation. Furthermore, structural analyses showed that E27 recruits STAT2 through a bipartite binding interface, partially overlapping with the IRF9 binding site.
Article
Biochemistry & Molecular Biology
Francis J. O'Reilly, Andrea Graziadei, Christian Forbrig, Rica Bremenkamp, Kristine Charles, Swantje Lenz, Christoph Elfmann, Lutz Fischer, Joerg Stuelke, Juri Rappsilber
Summary: Accurately modeling protein structures and their complexes using AI is revolutionizing molecular biology. In this study, we used a combination of crosslinking mass spectrometry and CoFrac-MS to identify protein-protein interactions in a Gram-positive bacterium. By predicting the structures of these interactions and validating them with crosslinking MS data, we proposed novel structural models of protein assemblies and identified new interactors of important cellular machineries. This approach provides insights into protein-protein interactions inside intact cells and is applicable to genetically intractable organisms.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kolja Stahl, Andrea Graziadei, Therese Dau, Oliver Brock, Juri Rappsilber
Summary: Limitations of Alphafold2 structure prediction are overcome by incorporating experimentally determined distance constraints using AlphaLink. This modified version improves prediction for challenging targets by utilizing sparse experimental contacts and can predict distinct protein conformations. Experimental data on residue-residue contacts obtained through crosslinking mass spectrometry validates the improved performance. The noise-tolerant framework for integrating data allows accurate characterization of protein structures from in-cell data.
NATURE BIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Maria Lyngaas Torgersen, Peter J. Judge, Juan F. Bada Juarez, Abhilash D. Pandya, Markus Fusser, Charlie W. Davies, Matylda K. Maciejewska, Daniel J. Yin, Gunhild M. Maelandsmo, Tore Skotland, Anthony Watts, Kirsten Sandvig
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2020)
