Journal
MICROBIAL BIOTECHNOLOGY
Volume 3, Issue 4, Pages 403-411Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1751-7915.2009.00148.x
Keywords
-
Categories
Funding
- Swedish Research Council (VR)
- Carl Tryggers Stiftelse
- National Institutes of Health (NIH)
- EMBO YI
- The Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
Ask authors/readers for more resources
The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available