Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems

(2012) Stefan Haberstock et al.   PROTEIN EXPRESSION AND PURIFICATION

Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes

(2011) Takuma Ohtsuka et al.   ANALYTICAL BIOCHEMISTRY

Lipid–protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes

(2011) E.N. Lyukmanova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs

(2011) Jian-Ping Yang et al.   BMC BIOTECHNOLOGY

Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes

(2011) Yi Ma et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A PCR-directed cell-free approach to optimize protein expression using diverse fusion tags

(2011) Andrew V. Kralicek et al.   PROTEIN EXPRESSION AND PURIFICATION

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunitafold similar to the protein in the cell membrane

(2011) Eva-Maria E. Uhlemann et al.   PROTEIN SCIENCE

Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL

(2010) Catherine Berrier et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

α-Hemolysin pore formation into a supported phospholipid bilayer using cell-free expression

(2010) Jerome Chalmeau et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Atomistic Simulations of Bicelle Mixtures

(2010) Yong Jiang et al.   BIOPHYSICAL JOURNAL

Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation

(2010) Yuki Moritani et al.   FEBS Journal

Cell-free Synthesis and Functional Characterization of Sphingolipid Synthases from Parasitic Trypanosomatid Protozoa

(2010) Elitza S. Sevova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

(2010) Friederike Junge et al.   New Biotechnology

Engineering extended membrane scaffold proteins for self-assembly of soluble nanoscale lipid bilayers

(2010) Y. V. Grinkova et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Cell-free expression profiling of E. coli inner membrane proteins

(2010) Daniel Schwarz et al.   PROTEOMICS

Cell-free synthesis of functional aquaporin Z in synthetic liposomes

(2009) Norman T. Hovijitra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Charged liposome affects the translation and folding steps of in vitro expression of green fluorescent protein

(2009) Hiroshi Umakoshi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Incorporation of Antimicrobial Peptides into Membranes: A Combined Liquid-State NMR and Molecular Dynamics Study of Alamethicin in DMPC/DHPC Bicelles

(2009) Jens Dittmer et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Single-step production of functional OEP24 proteoliposomes

(2009) Lavinia Liguori et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of functional bacteriorhodopsin by anEscherichia colicell-free protein synthesis system supplemented with steroid detergent and lipid

(2009) Kazumi Shimono et al.   PROTEIN SCIENCE

Combined localization and real-time functional studies using a GFP-tagged ABCG2 multidrug transporter

(2008) Tamás I. Orbán et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

High yield cell-free production of integral membrane proteins without refolding or detergents

(2008) Jessica J. Wuu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Large-scale production of functional membrane proteins

(2008) F. Junge et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Diacylglycerol Specifically Blocks Spontaneous Integration of Membrane Proteins and Allows Detection of a Factor-assisted Integration

(2008) Yosuke Kawashima et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins

(2008) Lavinia Liguori et al.   JOURNAL OF CONTROLLED RELEASE

In Vitro Multimerization and Membrane Insertion of Bacterial Outer Membrane Secretin PulD

(2008) Ingrid Guilvout et al.   JOURNAL OF MOLECULAR BIOLOGY

Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach

(2008) Federico Katzen et al.   JOURNAL OF PROTEOME RESEARCH

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles

(2008) Jenny A. Cappuccio et al.   MOLECULAR & CELLULAR PROTEOMICS

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

(2008) Michael A. Goren et al.   PROTEIN EXPRESSION AND PURIFICATION

Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis

(2007) Shin-ichiro M. Nomura et al.   JOURNAL OF BIOTECHNOLOGY

Development of a Minimal Cell-Free Translation System for the Synthesis of Presecretory and Integral Membrane Proteins

(2005) Yutetsu Kuruma et al.   BIOTECHNOLOGY PROGRESS