Article
Environmental Sciences
Lauris Evariste, Antoine Mottier, Eric Pinelli, Emmanuel Flahaut, Laury Gauthier, Florence Mouchet
Summary: The interest in graphene-based nanomaterials (GBMs) is increasing globally due to their innovative applications, but concerns about their potential adverse effects on the environment and endocrine disruption potential are also growing. A study found that graphene oxide (GO) and reduced graphene oxide (rGO) have different effects on amphibian endocrine systems, with GBMs potentially increasing the bioavailability and toxicity of other pollutants through hormone adsorption. This highlights the need for further research on the environmental impact and potential risks associated with GBMs.
Review
Endocrinology & Metabolism
Yun-Bo Shi
Summary: Thyroid hormone T3 plays a critical role in organ function, metabolism, and animal development, especially during the neonatal period in mammals. Studies on anuran metamorphosis in amphibians have shed light on how T3 regulates vertebrate development. The absence of T3 receptors in tadpoles leads to premature development of adult tissues and eventually lethality, highlighting the importance of TRs in mediating T3 effects.
Article
Multidisciplinary Sciences
Sayumi Oishi, Minoru Moriyama, Masaki Mizutani, Ryo Futahashi, Takema Fukatsu
Summary: This study investigates the remodeling mechanism of symbiotic organs and its impact on microbial symbionts during insect metamorphosis. By manipulating the metamorphosis process in stinkbugs, the researchers discovered that the symbiotic organ transformed from nymph type to adult type under the control of the MEKRE93 pathway. The remodeling of the symbiotic organ not only affects the retention function of symbionts but also extends its role in food digestion. Additionally, the symbiotic bacteria in the adult-type symbiotic organ were found to upregulate genes associated with the production of sulfur-containing amino acids, indicating their specific function in host reproduction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
Rikke Poulsen, Shireen H. Jackman, Martin Hansen, Caren C. Helbing
Summary: Anuran metamorphosis is associated with changes in thyroid hormone levels and gene expression. New technology allows for measuring TH levels and gene transcription during metamorphosis. The concentration profile of T2 during metamorphosis suggests its role in anuran development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Environmental Sciences
ZhiChao Dang
Summary: Both AMA and LAGDA are used to detect thyroid-mediated modality and adversity based on morphological changes during thyroid hormone-dependent metamorphosis. The EU Guidance recommends their use for investigating the effects of thyroid hormone system disrupting chemicals. However, there is debate over the necessity of using LAGDA as a follow-up to positive AMA results. This study analyzes the similarities, differences, and sensitivity of these two assays in detecting TDCs.
ENVIRONMENTAL POLLUTION
(2022)
Article
Toxicology
Douglas J. Fort, M. Annegaaike Leopold, Jeffrey C. Wolf, Kevin J. Todhunter, Peter J. J. M. Weterings
Summary: The present study evaluated the impact of dietary quality on the effects of chemical stressors on premetamorphic development and metamorphosis in tadpoles. The results showed that suboptimal dietary quality negatively affected both growth and metamorphic development, and iodine supplementation only partially compensated for the effects on metamorphosis.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Cell Biology
Takashi Hasebe, Kenta Fujimoto, Atsuko Ishizuya-Oka
Summary: Recent research has shown that the Hippo signaling pathway plays an important role in intestinal remodeling during amphibian metamorphosis. This study found that the expression of genes involved in the Hippo pathway is regulated by thyroid hormone (TH) in the metamorphosing intestine of Xenopus laevis. The transcriptional co-activator YAP1, a major effector of the Hippo signaling, is expressed in adult stem cells and surrounding connective tissue cells. The interaction between YAP1 and its binding partner TEAD1 is inhibited by verteporfin (VP), and this inhibition leads to decreased stem cell number and proliferation, resulting in the failure of adult epithelial development.
CELL AND TISSUE RESEARCH
(2022)
Article
Cell Biology
Yuki Shibata, Yuta Tanizaki, Hongen Zhang, Hangnoh Lee, Mary Dasso, Yun-Bo Shi
Summary: Research shows that TR genes play crucial roles in larval epithelial cell death and adult stem cell formation during anuran metamorphosis. Lack of these processes in TRDKO tadpoles results in inhibition of metamorphosis progression.
Article
Endocrinology & Metabolism
E. M. Koide, E. A. Abbott, C. C. Helbing
Summary: Thyroid hormone (TH) plays a critical role in vertebrate development by regulating gene expression and potentially through a molecular memory mechanism. This study investigates the early steps of TH signal transduction and identifies potential components of the molecular memory using the American bullfrog as a model. RNA-seq analysis reveals complex gene expression changes under TH exposure at low temperature, suggesting a more intricate molecular memory than previously believed. The findings suggest that changes in RNA stability, rather than transcription or translation, may contribute to the molecular memory. Manipulating temperature and TH response in cultured organs provide a valuable opportunity for further studying early TH signaling mechanisms.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Muriel Rigolet, Nicolas Buisine, Marylou Scharwatt, Evelyne Duvernois-Berthet, Daniel R. Buchholz, Laurent M. Sachs
Summary: Thyroid hormones and glucocorticoids play a role in cell-cycle regulation through the joint regulation of genes in the liver of tadpoles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Kenta Fujimoto, Yuki Shibata, Takashi Hasebe
Summary: This study investigates the molecular mechanisms involved in postembryonic maturation of the vertebrate intestine using thyroid hormone (TH)-dependent intestinal remodeling during amphibian metamorphosis as a model. The study reveals the significant role of signaling pathways, such as SHH/BMP4, WNT, Notch, and Hippo, in stem cell regulation during this process.
JOURNAL OF NIPPON MEDICAL SCHOOL
(2023)
Article
Biology
Yaxin Shi, Yang Shi, Wenyao Ji, Xike Li, Zhiyi Shi, Jilun Hou, Wenjuan Li, Yuanshuai Fu
Summary: In this study, the role of thyroid hormones (TH) in visual remodeling during Japanese flounder (Paralichthys olivaceus) metamorphosis was investigated through cellular molecular biology experiments. The results showed that the expression of the five opsin genes of the flounder were highest in eye tissue and varied with the metamorphosis process. The expression of rh1, sws2a beta and lws was positively regulated by exogenous TH, but inhibited by thiourea (TU) compared to the control group. Meanwhile, T3 levels in flounder larvae were increased by TH and decreased by TU. Based on the differences in the expression of the three isoforms of the thyroid hormone receptor (TR) (Tr alpha a, Tr alpha b and Tr beta), it was further hypothesized that T3 may directly or indirectly regulate the expression of sws2a beta through Tr alpha a. This study demonstrates the regulatory role of TH in opsins during flounder metamorphosis and provides a basis for further investigation on the molecular mechanisms underlying the development of the retinal photoreceptor system in flounders.
Article
Multidisciplinary Sciences
Fang Ye, Guodong Zhang, E. Weigao, Haide Chen, Chengxuan Yu, Lei Yang, Yuting Fu, Jiaqi Li, Sulei Fu, Zhongyi Sun, Lijiang Fei, Qile Guo, Jingjing Wang, Yanyu Xiao, Xinru Wang, Peijing Zhang, Lifeng Ma, Dapeng Ge, Suhong Xu, Juan Caballero-Perez, Alfredo Cruz-Ramirez, Yincong Zhou, Ming Chen, Ji-Feng Fei, Xiaoping Han, Guoji Guo
Summary: This study developed a new method of single-cell sequencing to analyze the perturbation patterns of cell type-related gene expression in neotenic and metamorphosed axolotls. By analyzing gene expression of over 1 million single cells, the first adult axolotl cell landscape was constructed. Comparison of single-cell transcriptomes between tissues of neotenic and metamorphosed axolotls revealed the heterogeneity of non-immune parenchymal cells in different tissues and identified their regulatory network.
NATURE COMMUNICATIONS
(2022)
Article
Gastroenterology & Hepatology
Paul Manka, Jason D. Coombes, Svenja Sydor, Marzena K. Swiderska-Syn, Jan Best, Karine Gauthier, Leo A. van Grunsven, Ye H. Oo, Cindy Wang, Anna M. Diehl, Georg S. Hoenes, Lars C. Moeller, Anja Figge, Rene J. Boosman, Klaas N. Faber, Andrea Tannapfel, Oliver Goetze, Patricia Aspichueta, Christian M. Lange, Ali Canbay, Wing-Kin Syn
Summary: Thyroid hormones play a crucial role in hepatic fibrogenesis by regulating the phenotype of hepatic stellate cells (HSC) via the TGF beta signaling pathway. In vitro experiments showed that T3 can inhibit the expression of fibrogenic genes and promote wound healing in HSC. In vivo experiments on TR alpha-deficient mice further confirmed the importance of the TH-TR axis in liver fibrosis.
LIVER INTERNATIONAL
(2023)
Article
Ecology
Marissa Fabrezi, Julio Cesar Cruz
Summary: Studies on the effects of thyroid hormones on larval development in frogs provide insights into the genetic and hormonal mechanisms driving growth and transitions throughout development. Mesocosm experiments offer realistic data on environmental conditions, critical for understanding anuran larval development and endocrine disruption. Thyroid axis disruption, specifically with methimazole and thyroxine, influences developmental rates and musculoskeletal morphogenesis in premetamorphic stages of tadpoles.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Genetics & Heredity
Bridget D. DeLay, Mark E. Corkins, Hannah L. Hanania, Matthew Salanga, Jian Min Deng, Norihiro Sudou, Masanori Taira, Marko E. Horb, Rachel K. Miller
Article
Genetics & Heredity
Bridget D. DeLay, Mark E. Corkins, Hannah L. Hanania, Matthew Salanga, Jian Min Deng, Norihiro Sudou, Masanori Taira, Marko E. Horb, Rachel K. Miller
Article
Genetics & Heredity
Mark E. Corkins, Hannah L. Hanania, Vanja Krneta-Stankic, Bridget D. DeLay, Esther J. Pearl, Moonsup Lee, Hong Ji, Alan J. Davidson, Marko E. Horb, Rachel K. Miller
Article
Multidisciplinary Sciences
Jeffrey D. Steimle, Scott A. Rankin, Christopher E. Slagle, Jenna Bekeny, Ariel B. Rydeen, Sunny Sun-Kin Chan, Junghun Kweon, Xinan H. Yang, Kohta Ikegami, Rangarajan D. Nadadur, Megan Rowton, Andrew D. Hoffmann, Sonja Lazarevic, William Thomas, Erin A. T. Boyle Anderson, Marko E. Horb, Luis Luna-Zurita, Robert K. Ho, Michael Kyba, Bjarke Jensen, Aaron M. Zorn, Frank L. Conlon, Ivan P. Moskowitz
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Makoto Nakamura, Hitoshi Yoshida, Eri Takahashi, Marcin Wlizla, Kimiko Takebayashi-Suzuki, Marko E. Horb, Atsushi Suzuki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Cell Biology
Jean A. Smith, Ennessa G. Curry, R. Eric Blue, Christine Roden, Samantha E. R. Dundon, Anthony Rodriguez-Vargas, Danielle C. Jordan, Xiaomin Chen, Shawn M. Lyons, John Crutchley, Paul Anderson, Marko E. Horb, Amy S. Gladfelter, Jimena Giudice
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Allan F. Wiechmann, Teryn A. Martin, Marko E. Horb
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Thomas Naert, Dieter Tulkens, Nicole A. Edwards, Marjolein Carron, Nikko-Ideen Shaidani, Marcin Wlizla, Annekatrien Boel, Suzan Demuynck, Marko E. Horb, Paul Coucke, Andy Willaert, Aaron M. Zorn, Kris Vleminckx
SCIENTIFIC REPORTS
(2020)
Article
Developmental Biology
Kelsey Coppenrath, Andre L. P. Tavares, Nikko-Ideen Shaidani, Marcin Wlizla, Sally A. Moody, Marko Horb
Summary: The Six1 gene plays a crucial role in vertebrate organ development, and its mutation leads to severe disruptions in the morphology of multiple organs and contributes to the formation of related diseases.
Article
Developmental Biology
Natalya Zahn, Christina James-Zorn, Virgilio G. Ponferrada, Dany S. Adams, Julia Grzymkowski, Daniel R. Buchholz, Nanette M. Nascone-Yoder, Marko Horb, Sally A. Moody, Peter D. Vize, Aaron M. Zorn
Summary: Normal tables and illustrations of embryonic development play a crucial role in research and teaching. However, due to copyright restrictions, the use of classic illustrations is no longer possible. To address this, we have created a new resource consisting of 133 high-quality illustrations and a landmarks table, which will support future Xenopus research and teaching.
Article
Developmental Biology
Douglas W. Houston, Karen L. Elliott, Kelsey Coppenrath, Marcin Wlizla, Marko E. Horb
Summary: Asymmetric signalling centres are crucial for early axis formation in vertebrates. The study finds that Wnt11b is involved in the distribution of dorsal determinants and plays a key role in dorsal axis formation and embryonic development.
Article
Biochemistry & Molecular Biology
Marco Morselli, Ronan Bennett, Nikko-Ideen Shaidani, Marko Horb, Leonid Peshkin, Matteo Pellegrini
Summary: This study finds that age-associated changes in DNA methylation also exist in amphibians, and whole-genome bisulfite sequencing (WGBS) profiles from skin samples of nine frogs were used to characterize the gene- and chromosome-scale features of age-associated DNA methylation changes. The results suggest that the mechanism of DNA methylation changes in amphibians is similar to that in mammals. Additionally, a targeted DNA methylation analysis (TBSseq) assay was developed for future studies with larger cohorts of individuals. The findings of this study will contribute to understanding the relationship between DNA methylation and other hallmarks of aging using the unique resources available for Xenopus.
Article
Genetics & Heredity
Benjamin L. S. Furman, Caroline M. S. Cauret, Martin Knytl, Xue-Ying Song, Tharindu Premachandra, Caleb Ofori-Boateng, Danielle C. Jordan, Marko E. Horb, Ben J. Evans
Review
Physiology
Marko Horb, Marcin Wlizia, Anita Abu-Daya, Sean McNamara, Dominika Gajdasik, Takeshi Igawa, Atsushi Suzuki, Hajime Ogino, Anna Noble, Jacques Robert, Christina James-Zorn, Matthew Guille, Morgane Nicolas, Thomas Lafond, Daniel Boujard, Yann Audic, Brigitte Guillet, Akihiko Kashiwagi, Keiko Kashiwagi, Nanoka Suzuki, Ichiro Tazawa, Haruki Ochi, Nobuaki Furuno, Minoru Takasc, Keisuke Nakajima, Hicleki Hanada, Ikuo Miura, Atsushi Kurabayashi, Takashi Kato, Kei Sato, Kimiko Takcbayashi-Suzuki, Hitoshi Yoshida
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Developmental Biology
Wil Ratzan, Rosalia Falco, Cristy Salanga, Matthew Salanga, Marko E. Horb
DEVELOPMENTAL BIOLOGY
(2017)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)