Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Shu-An Hsieh, Danial Shamsaei, Derek R. Eitzmann, Jared L. Anderson
Summary: This study demonstrates the use of a 3D printed droplet generation platform coupled with molecular beacons in LAMP detection, allowing for low-cost and customizable droplet generation. The study also demonstrates the use of a smartphone-based imaging system to increase accessibility. This method can be used for sequence-specific, sensitive, and accurate DNA quantification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jingyang Zhang, Yu Wang, Xudong Zhao, Mengmeng Chen, Yuan Peng, Jialei Bai, Shuang Li, Dianpeng Han, Shuyue Ren, Kang Qin, Sen Li, Tie Han, Zhixian Gao
Summary: The development of a dual sensitization smartphone colorimetric strategy based on rolling circle amplification (RCA) coils gathering Au tetrahedra has been successful. This strategy utilizes the dual sensitization effect of RCA and Au tetrahedra to amplify the signal. The use of ImageJ software allows for timely target detection under practical conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuyu Zheng, Jinpeng Wang, Genwang Chen, Meie Wang, Tebin Chen, Qiaohong Ke, Yajun Huang, Fan Cai, Rongfu Huang, Chunmei Fan
Summary: An innovative and simple electrochemical aptasensing platform was designed for the voltammetric detection of prostate-specific antigen (PSA) in biological fluids. The method showed good sensitivity and reproducibility, and yielded consistent results compared to the traditional enzyme-linked immunosorbant assay (ELISA) method. This technique provides a new possibility for the quantitative monitoring of low-concentration biomarkers or nucleic acids.
Article
Chemistry, Multidisciplinary
Jing Wang, Shanshan Yu, Qiong Wu, Xue Gong, Shizhen He, Jinhua Shang, Xiaoqing Liu, Fuan Wang
Summary: We designed a self-catabolic DNA nanocapsule for sustained activation of therapeutic DNAzyme in cancer cells, utilizing a hierarchical rolling circle replication (RCR) nanostructure. Through tumor-anchoring aptamer strands, the nanocapsule was specifically accumulated in cancer cells and sequentially activated for gene silencing. By employing a programmable RCR assembly strategy, our DNAzyme nanoplatform shows promise for developing smart gene therapeutics and personalized nanomedicines.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Masanobu Nagano, Takumi Toda, Kurumi Makino, Hiroko Miki, Yoshiaki Sugizaki, Hideyuki Tomizawa, Atsunobu Isobayashi, Keitaro Yoshimoto
Summary: Researchers have developed an MTX detection system using chemically synthesizable homogeneous oligonucleotides. Through microbead-assisted capillary electrophoresis, they successfully identified MSmt7 with a similar level of specificity to antiMTX antibodies. This oligonucleotide can be coupled with a peroxidase-like hemin-DNAzyme to construct a bifunctional MTX sensing method with a low detection limit.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Bingzhi Li, Anqi Xia, Siying Xie, Lei Lin, Zhirun Ji, Tiying Suo, Xing Zhang, He Huang
Summary: A signal-amplified detection method of FEN1 based on cleavage-induced ligation and rolling circle amplification was developed in this study, achieving highly sensitive and specific detection of FEN1 in real samples, as well as evaluating the inhibitory effects of chemicals on FEN1. This novel biosensor method enriches the tools for FEN1-based diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Mai Zhang, Honghong Wang, Hui Wang, Fangfang Wang, Zhengping Li
Summary: A CRISPR/Cas12a-assisted sequence-specific detection method for LAMP products was developed to eliminate non-specific amplification effects. By designing target-specific stem-loop DNA probes, primer design for LAMP was greatly simplified, achieving sensitive detection of miRNAs with high specificity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yanfei Zhang, Jun Chen, Huihui Yang, Wen Yin, Chunrong Li, Yuzhi Xu, Si-Yang Liu, Zong Dai, Xiaoyong Zou
Summary: The LCR-HCR method uses exosomes as vehicles for controlled release and recruitment, significantly improving miRNA imaging contrast by confining reactants on exosome vehicles. It provides a powerful tool for sensitive miRNA imaging in living cells and cancer diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Chi Yao, Junhan Ou, Jianpu Tang, Dayong Yang
Summary: Facing the increasing demand for precision medicine, materials chemistry systems with accurate molecular design, controllable structure, and adjustable biological activity are required for bioanalysis. In recent years, DNA has been considered a potential biomaterial for analysis and has been applied in various fields. DNA supramolecular assembly on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules and enhance their functions in bioanalysis. This research direction is expected to guide the construction of more bioanalytical materials and promote the development of precision medicine.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Lei He, Xiaocheng Yu, Rongrong Huang, Lian Jin, Yuan Liu, Yan Deng, Song Li, Hui Chen, Zhu Chen, Zhiyang Li, Pengfeng Xiao, Nongyue He
Summary: A highly specific and sensitive fluorescence scheme based on padlock probe-based exponential rolling circle amplification (P-ERCA) assay for detecting extracellular vesicles derived from lung cancer cells was reported. The method exhibits high specificity and sensitivity, and successfully distinguishes between lung cancer patients and healthy individuals in real human serum samples.
Article
Dermatology
Ji Yeon Hong, Tae-Rin Kwon, Jong Hwan Kim, Byung Chul Lee, Beom Joon Kim
JOURNAL OF COSMETIC DERMATOLOGY
(2020)
Article
Multidisciplinary Sciences
Jong Hwan Kim, Tae-Rin Kwon, Sung Eun Lee, Yoo Na Jang, Hye Sung Han, Seog Kyun Mun, Beom Joon Kim
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Ki-Cheol Han, Daechan Park, Shinyeong Ju, Young Eun Lee, Sun-Hee Heo, Young-Ae Kim, Ji Eun Lee, Yuna Lee, Kyong Hwa Park, Se-Ho Park, Hee Jin Lee, Cheolju Lee, Mihue Jang
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Jinhong Cho, Jinyoung Park, Sang Chul Shin, Mihue Jang, Jae-Hong Kim, Eunice EunKyeong Kim, Eun Joo Song
Article
Dermatology
Kwang Ho Yoo, Tae Rin Kwon, Jong Hwan Kim, Beom Joon Kim
Summary: The study evaluated the efficacy and safety of treating lower eyelid fat bulging with ultrasound-assisted lipolysis (UAL) on a Yorkshire pig. Treatment with UAL successfully reduced fat bulging and increased collagen contraction, suggesting it may be a beneficial and well-tolerated option.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Dermatology
Tae-Rin Kwon, Jong Hwan Kim, You Na Jang, Sung-Eun Lee, Ji-Yeon Hong, Kwang Ho Yoo, Beom Joon Kim
Summary: This study compared the treatment results of two parameters with different energy outputs in an in vivo porcine model, and found that 1,060 nm laser energy at 0.9 and 1.4 W/cm(2) caused significant changes in the density of the fat layer. Blood chemistry analysis, histological examination, and TUNEL assays were used to determine the mechanism of action, efficacy, and safety of the procedure.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Hyojin Lee, Tae Hee Kim, Daechan Park, Mihue Jang, Justin J. Chung, Soo Hyun Kim, Sang-Heon Kim, Kwan Hyi Lee, Youngmee Jung, Seung Ja Oh
Article
Dermatology
Ji Yeon Hong, Guk Jin Jeong, Tae-Rin Kwon, Jong Hwan Kim, Kapsok Li, Beom Joon Kim
Summary: The study found that prabotulinum toxin A can effectively improve lower face contour, with an optimal dose of 48 to 72 units showing significant results after 12 weeks. However, a dose of 24 units may be insufficient for resolving square face problems. Participants in Group E reported discomfort during jaw movement.
DERMATOLOGIC SURGERY
(2021)
Article
Dermatology
Ji Yeon Hong, Jong Hwan Kim, Tae-Rin Kwon, Jun Ki Hong, Kapsok Li, Beom Joon Kim
Summary: Dermal fillers, particularly polycaprolactone (PCL), have gained popularity for skin rejuvenation, but current limitations include irritation and nonhomogeneous particle suspensions. A novel particle-free PCL filler, DLMR01, was developed to address these issues, showing promising results in terms of safety, biodegradability, and neocollagenesis in a rat model.
DERMATOLOGIC THERAPY
(2021)
Article
Engineering, Biomedical
Byoungjae Kim, Young Eun Lee, Ji Woo Yeon, Ga-Yeon Go, Junhyoung Byun, Kijeong Lee, Hyomin K. Lee, Junho K. Hur, Mihue Jang, Tae Hoon Kim
Summary: This study established a DC-based therapeutic approach using CRISPR/Cas9-mediated targeted gene disruption to alleviate allergic reactions associated with allergic rhinitis. Targeting VPS37A/B enabled reduction of Th2 cytokine production and alleviation of AR-related symptoms in mouse models, providing a promising strategy for treating allergic diseases.
Article
Oncology
Jonghwan Kim, Soyoung Chun, Seul-Ong Ohk, Sanghoon Kim, Juwan Kim, Sungoh Lee, Hangyu Kim, Sujong Kim
Summary: The study demonstrated the protective effects of food-polydeoxyribonucleotides (f-PDRNs) in a rat model of gastric mucosal injury, showing reduction in gastric acid secretion and promotion of tissue repair. This suggests that f-PDRNs as a food supplement may serve as an effective approach for treating gastritis.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Dermatology
Hye Sung Han, Jong Hwan Kim, Tae-Rin Kwon, Sung Eun Lee, Kwang Ho Yoo, Kapsok Li, Hyeonji Park, Joon Ho Bae, Su Jeong Park, Jisun Han, Suyeon Kim, Beom Joon Kim, You Na Jang
Summary: The study found a moderate correlation between the roughness parameter values obtained using the PRIMOS system and the clinical roughness grade. This suggests that PRIMOS could be a valuable tool for clinicians and researchers to conduct accurate and objective patient assessments.
SKIN RESEARCH AND TECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
Young Eun Lee, Chae Min Yuk, Minseok Lee, Ki-Cheol Han, Eunsung Jun, Tae Sung Kim, Ja-Lok Ku, Sung G. Im, Eunjung Lee, Mihue Jang
Summary: This study introduces a novel 3D assay platform for monitoring NK-mediated cancer cell killing, offering a new approach for studying cancer therapy.
BIOMATERIALS SCIENCE
(2022)
Article
Oncology
Young Eun Lee, Ga-Yeon Go, Eun-Young Koh, Han-Na Yoon, Minkoo Seo, Seung-Mo Hong, Ji Hye Jeong, Jin-Chul Kim, Duck Cho, Tae Sung Kim, Song Cheol Kim, Eunsung Jun, Mihue Jang
Summary: Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) impair the functionality of natural killer (NK) cells, which are considered as a promising therapeutic modality. Targeted therapies against CAFs may enhance NK-mediated cancer killing.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Pharmacology & Pharmacy
Jeong-Hwan Kim, Chang-Taek Oh, Tae-Rin Kwon, Jong Hwan Kim, Dong-Ho Bak, Hyuk Kim, Won-Seok Park, Beom Joon Kim
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
(2020)