Computational Library Design for Increasing Haloalkane Dehalogenase Stability

Computationally designed libraries for rapid enzyme stabilization

(2014) H. J. Wijma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel

(2013) Tana Koudelakova et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Conformational dynamics of xylanase a fromStreptomyces lividans: Implications for TIM-barrel enzyme thermostability

(2013) Yanrui Ding et al.   BIOPOLYMERS

Organic co-solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases

(2013) Veronika Stepankova et al.   Biotechnology Journal

Structure- and sequence-analysis inspired engineering of proteins for enhanced thermostability

(2013) Hein J Wijma et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Computational design gains momentum in enzyme catalysis engineering

(2013) Hein J. Wijma et al.   FEBS Journal

Crystal Structure and Site-Directed Mutagenesis Analyses of Haloalkane Dehalogenase LinB from Sphingobium sp. Strain MI1205

(2013) M. Okai et al.   JOURNAL OF BACTERIOLOGY

Enhanced thermostability of keratinase by computational design and empirical mutation

(2013) Baihong liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Variations in the stability of NCR ene reductase by rational enzyme loop modulation

(2013) Sabrina Reich et al.   JOURNAL OF STRUCTURAL BIOLOGY

Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method

(2013) Xiangfei Song et al.   PLoS Computational Biology

Novel Protease Inhibitors via Computational Redesign of Subtilisin BPN′ Propeptide

(2012) Ashley B. Daugherty et al.   BIOCHEMISTRY

Directed enzyme evolution: beyond the low-hanging fruit

(2012) Moshe Goldsmith et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A Single Mutation in a Tunnel to the Active Site Changes the Mechanism and Kinetics of Product Release in Haloalkane Dehalogenase LinB

(2012) Lada Biedermannová et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

SS-Stabilizing Proteins Rationally: Intrinsic Disorder-Based Design of Stabilizing Disulphide Bridges in GFP

(2012) Bogdan S. Melnik et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Improved thermostability of AEH by combining B-FIT analysis and structure-guided consensus method

(2012) Janna K. Blum et al.   JOURNAL OF BIOTECHNOLOGY

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Role of Active Site Rigidity in Activity: MD Simulation and Fluorescence Study on a Lipase Mutant

(2012) Md. Zahid Kamal et al.   PLoS One

Assessing Predictors of Changes in Protein Stability upon Mutation Using Self-Consistency

(2012) Grant Thiltgen et al.   PLoS One

Engineering a Disulfide Bond in the Lid Hinge Region of Rhizopus chinensis Lipase: Increased Thermostability and Altered Acyl Chain Length Specificity

(2012) Xiao-Wei Yu et al.   PLoS One

Automated selection of stabilizing mutations in designed and natural proteins

(2012) B. Borgo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59

(2012) O. Khersonsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biophysical characterization of mutants ofBacillus subtilislipase evolved for thermostability: Factors contributing to increased activity retention

(2012) Wojciech Augustyniak et al.   PROTEIN SCIENCE

Haloalkane dehalogenase catalysed desymmetrisation and tandem kinetic resolution for the preparation of chiral haloalcohols

(2012) Alja Westerbeek et al.   TETRAHEDRON

Kinetic Resolution of α-Bromoamides: Experimental and Theoretical Investigation of Highly Enantioselective Reactions Catalyzed by Haloalkane Dehalogenases

(2011) Alja Westerbeek et al.   ADVANCED SYNTHESIS & CATALYSIS

A Simple Enantioconvergent and Chemoenzymatic Synthesis of Optically Active α-Substituted Amides

(2011) Wiktor Szymański et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Exceptional thermal stability and organic solvent tolerance of an esterase expressed from a thermophilic host

(2011) Yuxia Mei et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed Evolution Strategies for Enantiocomplementary Haloalkane Dehalogenases: From Chemical Waste to Enantiopure Building Blocks

(2011) Jan G. E. van Leeuwen et al.   CHEMBIOCHEM

Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering

(2011) Umut Y. Ulge et al.   NUCLEIC ACIDS RESEARCH

Computational design and selections for an engineered, thermostable terpene synthase

(2011) Juan E. Diaz et al.   PROTEIN SCIENCE

Computational protein design with explicit consideration of surface hydrophobic patches

(2011) Ron Jacak et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Mathematics, Thermodynamics, and Modeling to Address Ten Common Misconceptions about Protein Structure, Folding, and Stability

(2010) Srebrenka Robic   CBE-Life Sciences Education

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

(2010) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein engineering of microbial enzymes

(2010) Dominique Böttcher et al.   CURRENT OPINION IN MICROBIOLOGY

A comparison of different initialization protocols to obtain statistically independent molecular dynamics simulations

(2010) Samuel Genheden et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Role of conformational sampling in computing mutation-induced changes in protein structure and stability

(2010) Elizabeth H. Kellogg et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Enhancing thermostability of a Rhizomucor miehei lipase by engineering a disulfide bond and displaying on the yeast cell surface

(2009) Zhen-lin Han et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Organic solvent-tolerant enzymes

(2009) Noriyuki Doukyu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Functions and evolution of selenoprotein methionine sulfoxide reductases

(2009) Byung Cheon Lee et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Stability effects of mutations and protein evolvability

(2009) Nobuhiko Tokuriki et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility

(2009) Garrett M. Morris et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Rational stabilization of enzymes by computational redesign of surface charge–charge interactions

(2009) Alexey V. Gribenko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessing computational methods for predicting protein stability upon mutation: good on average but not in the details

(2009) V. Potapov et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Enzymatic synthesis of α-2-deoxyglucosyl derivatives catalyzed by organic solvent-resistant α-glucosidase

(2008) Young-Min Kim et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Enzyme stability and stabilization—Aqueous and non-aqueous environment

(2008) Padma V. Iyer et al.   PROCESS BIOCHEMISTRY

Dynameomics: Large-scale assessment of native protein flexibility

(2008) Noah C. Benson et al.   PROTEIN SCIENCE

How Protein Stability and New Functions Trade Off

(2008) Nobuhiko Tokuriki et al.   PLoS Computational Biology