Article
Biotechnology & Applied Microbiology
Yifan Yang, Xiaoping Fu, Xingya Zhao, Jianyong Xu, Yihan Liu, Hongchen Zheng, Wenqin Bai, Hui Song
Summary: A carbohydrate binding module 68 (CBM68) was used to enhance the secretory expression of a thermostable alpha-amylase (BLA702) in Bacillus subtilis. The extracellular activity of BLA702 guided by CBM68 was significantly higher than that guided by its original signal peptide. By constructing an engineered strain, the extracellular amylase activity was further improved and showed better stability. This new strain has potential for industrial production of BLA702.
FERMENTATION-BASEL
(2023)
Article
Agriculture, Multidisciplinary
Li Liangfei, Zhu Yafeng, Xu Kai, Xu Zheng
Summary: The study demonstrated efficient production of epilactose using a food-grade host expressing a novel CE enzyme, and an efficient and low-cost process for separation. Additionally, a production process for a mixed syrup containing epilactose and d-tagatose from lactose was established for the first time.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Zhen Huang, Guorong Ni, Fei Wang, Xiaoyan Zhao, Yunda Chen, Lixia Zhang, Mingren Qu
Summary: This study cloned and expressed a lichenase gene (CelA203) from Bacillus subtilis B110 and characterized its properties. The CelA203 enzyme exhibited high stability and catalytic activity, showing great potential for oligosaccharide production in animal feed and brewing industries.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Rania Hammami, Maroua Oueslati, Marwa Smiri, Souhaila Nefzi, Mustapha Ruissi, Francesca Comitini, Gianfranco Romanazzi, Santa Olga Cacciola, Najla Sadfi Zouaoui
Summary: In this study, yeast and bacterial isolates with antagonistic activity against Penicillium digitatum and P. italicum on citrus fruits were screened and identified. These isolates significantly reduced the incidence of green and blue mold caused by these pathogens on citrus fruits, and effectively prevented fungal infection during low-temperature storage. Moreover, they did not have any negative effect on fruit quality and shelf life. These selected isolates have multiple modes of action and show promise as biocontrol agents for postharvest green and blue molds of citrus fruits.
Article
Biotechnology & Applied Microbiology
Qian Pi, Zhubing Zhu, Lei Tang
Summary: A new superfamily of heme-containing peroxidases, DyPs, has been discovered to efficiently decolorize various dyes, especially anthraquinone dyes. Using TfuDyP, it was found that the attack site on RB19 was identified and the incomplete degradation products of RB19 inhibited the growth of Bacillus subtilis. These findings provide a novel understanding of DyPs catalysis to anthraquinone dyes.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Xingxiang Xi, Kefeng Ni, Helong Hao, Yuepeng Shang, Bo Zhao, Zhen Qian
Summary: The environmental threat of plastic waste disposal has been a major concern, and recycling PET waste into reusable materials has become a solution. This study introduced a highly efficient PETase, BhrPETase, with superior hydrolyzing activity towards amorphous PET and remarkable thermostability, making it a promising candidate for PET recycling and bioremediation applications.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zidong Jiang, Lujia Zhang, Wei Zhou, Hui Li, Yuanyi Li, Weichuang Qin, Fengqing Wang, Dongzhi Wei, Bei Gao
Summary: In this study, a binding structure between SecA and a signal peptide was established, and a grinding approach was used to modify them, leading to improved protein secretion efficiency. A bidirectional grinding strategy was also developed to enhance the secretion system, resulting in a significantly increased protein activity. This research provides a novel and promising strategy for optimizing protein secretion and production in B. subtilis.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Mohamed A. Sobhy, Lingyun Zhao, Dalaver Anjum, Ali Behzad, Masateru Takahashi, Muhammad Tehseen, Alfredo De Biasio, Rachid Sougrat, Samir Hamdan
Summary: Thermostable enzymes have great potential in biotechnological applications. Cryo-electron microscopy was used to determine the high resolution structures of two thermostable enzymes with multimeric cage-like structures. These structures serve as models for studying biocatalysts and as benchmarks for cryo-EM sample preparation and reconstruction.
Article
Biotechnology & Applied Microbiology
Cong Fu, Xuexia Xu, Yukang Xie, Yufei Liu, Min Liu, Ai Chen, Jenny M. Blamey, Jiping Shi, Suwen Zhao, Junsong Sun
Summary: In this study, a novel microbial engineering platform for the fermentation production of l-fucose was established by introducing heterologous GDP-l-fucose synthesis pathway and engineering GDP-mannose mannosyl hydrolase (WcaH) in Bacillus subtilis. By rational design and mutagenesis, the efficiency of GDP-l-fucose hydrolysis by WcaH was enhanced. The engineered strain with WcaH(R36Y/N38R) produced 1.6 g/L l-fucose during shake-flask growth, which was 67.3% higher than wild-type WcaH. The accumulated l-fucose concentration in a 5 L bioreactor reached 6.4 g/L.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemical Research Methods
Kuidong Xu, Yi Tong, Yi Li, Jin Tao, Jianghua Li, Jingwen Zhou, Song Liu
Summary: A statistical model was developed to predict the effect of N-terminal coding sequence (NCS) on protein expression in Bacillus subtilis, leading to up to 515% increase or 79% decrease in the extracellular yield of B. subtilis pullulanase. This study provides a candidate tool for fine-tuning gene expression or enzyme production in B. subtilis through synonymous mutations and regression analysis.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agricultural Engineering
Kyoungseon Min, Hee Taek Kim, Si Jae Park, Siseon Lee, Ye Jean Jung, Jin-Suk Lee, Young Je Yoo, Jeong Chan Joo
Summary: This study explores a structure-based strategy to stabilize lipase A from Bacillus subtilis in a water-ethanol cosolvent, resulting in 4 mutants with higher stability in ethanol and potential applications in enzyme-based biorefineries for producing value-added fuels and chemicals.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Jingyuan Yang, Chao Wang, Qunqun Guo, Wenjun Deng, Guicai Du, Ronggui Li
Summary: A bacterial strain capable of secreting thermostable beta-glucosidase was isolated for GB production. Recombinant beta-glucosidase was obtained through genetic engineering and easily purified for industrial applications. This study provides a feasible method for producing GB and other industrial applications.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jingyuan Yang, Chao Wang, Qunqun Guo, Wenjun Deng, Guicai Du, Ronggui Lia
Summary: In this study, a bacterial strain capable of producing thermostable β-glucosidase was isolated from soil and identified as Bacillus altitudinis JYY-02. The recombinant β-glucosidase was successfully expressed in Escherichia coli and purified for industrial applications, such as the production of gardenia blue (GB) pigment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Ridwan Elemosho, Antonius Suwanto, Maggy Thenawidjaja
Summary: The successful extracellular expression of Geobacillus stearothermophilus lipase (T1.2RQ) in Bacillus subtilis WB800 using signal peptide of lipase A resulted in the highest extracellular yield. The enzyme was better expressed in rich media compared to minimal media, and an attempt to further increase expression by promoter optimization showed similar levels, indicating the need for downstream factor optimization.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Pulin Liu, Jingxiao Guo, Lihong Miao, Hanyan Liu
Summary: In this study, the secretion of feruloyl esterase was enhanced by optimizing codon usage and screening signal peptides. The genetically engineered B. subtilis successfully released ferulic acid from maize bran through solid-state fermentation, providing a promising candidate for ferulic acid production.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Chemistry, Multidisciplinary
Nana Ma, Zhifeng Chen, Jie Chen, Jingfei Chen, Cong Wang, Haifeng Zhou, Lishan Yao, Osami Shoji, Yoshihito Watanabe, Zhiqi Cong
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Biochemistry & Molecular Biology
Kun-Di Zhang, Wen Li, Ye-Fei Wang, Yan-Lin Zheng, Fang-Cheng Tan, Xiao-Qing Ma, Li-Shan Yao, Edward A. Bayer, Lu-Shan Wang, Fu-Li Li
Article
Chemistry, Multidisciplinary
Jingwen Li, Yefei Wang, Liaoyuan An, Jingfei Chen, Lishan Yao
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Biochemistry & Molecular Biology
Jia Wang, Jingfei Chen, Jingwen Li, Liaoyuan An, Yefei Wang, Qingshan Huang, Lishan Yao
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2018)
Article
Chemistry, Multidisciplinary
Xiangfei Song, Tianhang Lv, Jingfei Chen, Jia Wang, Lishan Yao
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Biochemical Research Methods
Jia Zhang, Yefei Wang, Baihui Chai, Jichao Wang, Lulu Li, Min Liu, Guang Zhao, Lishan Yao, Xiaolian Gao, Yifeng Yin, Jian Xu
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Xiangfei Song, Liaoyuan An, Mengting Wang, Jingfei Chen, Zhijun Liu, Lishan Yao
Summary: This study found that glycerol stabilizes protein folding, while betaine and taurine destabilize protein folding by affecting folding rates and instability. Betaine and taurine enhance quinary interactions between the protein and cellular environment, weakening electrostatic attraction in protein salt bridges, leading to protein destabilization in E. coli cells. These factors counteract the preferential exclusion mechanism used by osmolytes to stabilize proteins.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiangfei Song, Mengting Wang, Xiaoxu Chen, Xueying Zhang, Ying Yang, Zhijun Liu, Lishan Yao
Summary: This study investigated how the cellular environment affects electrostatic interactions between protein charges using in-cell nuclear magnetic resonance spectroscopy. The results showed that the transfer free energy in cells can modulate protein electrostatic interactions, with a larger effect on the interactions in the unfolded state compared to the folded state. This work highlights the importance of direct in-cell studies for understanding protein interactions and function.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Xiaoyan Liu, Mingming Dong, Yating Yao, Yan Wang, Jiawei Mao, Lianghai Hu, Lishan Yao, Mingliang Ye
Summary: This study presents an antibody-free approach to identify phosphotyrosine peptides by utilizing protein tyrosine phosphatase (PTP). By combining two Ti4+-IMAC-based phosphopeptide enrichment steps with PTP-catalyzed dephosphorylation, a cost-effective alternative for tyrosine phosphoproteomics analysis is provided.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Tingting Liu, Han Wu, Xiaofan Du, Jinzhi Wang, Zheng Chen, Hao Wang, Jinran Sun, Jianjun Zhang, Jiaping Niu, Lishan Yao, Jingwen Zhao, Guanglei Cui
Summary: This study presents a hydrated eutectic electrolyte with a water-locked effect, which strengthens the O-H bond of water via weak hydrogen bonding, leading to improved rechargeability and stability of aqueous sodium batteries.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Mengting Wang, Xiangfei Song, Jingfei Chen, Xiaoxu Chen, Xueying Zhang, Ying Yang, Zhijun Liu, Lishan Yao
Summary: Conformational dynamics of proteins play a crucial role in cellular functions. However, the influence of the intracellular environment on protein conformational dynamics is not well understood. In this study, the loop conformational dynamics of a model protein in Escherichia coli cells was investigated using nuclear magnetic resonance (NMR) spectroscopy. Weak interactions between the protein and surrounding macromolecules in cells hindered the rotational diffusion of the protein, allowing the detection of dynamics in the microsecond timescale using NMR spin relaxation method. The loop dynamics in the picosecond to microsecond range were confirmed using nanoparticle-assisted spin relaxation and residual dipolar coupling methods. Point mutation of the loop sequence perturbed the loop interactions with the intracellular environment. For the protein sequence with stronger interactions with surrounding macromolecules, the loop became more rigid in cells. In contrast, the effect of mutation on the loop dynamics in vitro was small. This study provides direct evidence that the intracellular environment can modify protein loop conformational dynamics through weak interactions.
Article
Chemistry, Analytical
Yefei Wang, Liaoyuan An, Ying Yang, Lishan Yao
ANALYTICAL CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Fengwei Li, Li Ma, Xingwang Zhang, Jingfei Chen, Feifei Qi, Yinyue Huang, Zepeng Qu, Lishan Yao, Wei Zhang, Eung-Soo Kim, Shengying Li
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Dong-Dong Meng, Xi Liu, Sheng Dong, Ye-Fei Wang, Xiao-Qing Ma, Haixia Zhou, Xinquan Wang, Li-Shan Yao, Yingang Feng, Fu-Li Li
BIOCHEMICAL JOURNAL
(2017)
Article
Chemistry, Multidisciplinary
Ning Zhang, Yefei Wang, Liaoyuan An, Xiangfei Song, Qingshan Huang, Zhijun Liu, Lishan Yao
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)