Article
Biochemistry & Molecular Biology
Kohki Ishida, Makoto Shimizu, Ayumi Wakasugi, Yuko Matsui, Akira Nakamura, Shuichi Kojima
Summary: In this study, a short peptide inhibitor against subtilisin BPN' was developed with high inhibitory activity and binding affinity.
Article
Biochemistry & Molecular Biology
Sunday N. Okafor, Pavimol Angsantikul, Hashim Ahmed
Summary: This research utilized computational tools to screen a large compound library and identified potential HIV-1 protease inhibitors. Two optimized molecules showed promising activity and should be further investigated in vitro and in clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Gulbag Kahlon, Rogelio Lira, Nikolay Masvlov, Emmett Pompa, Nimrat Brar, Scott Eagon, Marc O. Anderson, Armann Andaya, Jeff P. Chance, Hannah Fejzic, Aaron Keniston, Brian Vidal, Nicholas Trieu, Paulina Rodriguez, Jeremy P. Mallari
Summary: This study focused on investigating the function of the protease FLN in the protozoan parasite Plasmodium falciparum, which causes severe human malaria. The development of inhibitors targeting FLN showed significant improvements in potency against purified FLN and cultured P. falciparum, with computational studies predicting a binding model consistent with biochemical data.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Ismail Hasnaoui, Hassan Gwaithan, Taha Bilel Chalbi, Ennouamane Saalaoui, Abdeslam Asehraou, Alexandre Noiriel, Abdelkarim Abousalham, Hocine Hacene, Amel Bouanane-Darenfed, Marilize Le Roes-Hill, Bassem Jaouadi
Summary: In this study, a serine alkaline protease named SPSM was purified and characterized from Streptomyces mutabilis strain TN-X30. The SPSM gene was cloned and over-expressed in E. coli BL21(DE3)pLysS and E. coli BL21-AITM strains. The immobilization of SPSM using encapsulation and adsorption-encapsulation approaches and different carriers was investigated. The results showed that the immobilized SPSM exhibited similar characteristics to the soluble form, and the white clay and kaolin used as binders showed great potential in the immobilization of SPSM.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Casper Bojer Rasmussen, Carsten Scavenius, Ida B. Thogersen, Seandean Lykke Harwood, Oivind Larsen, Gro Elin Kjaereng Bjerga, Peter Stougaard, Jan J. Enghild, Mariane Schmidt Thogersen
Summary: In this study, a novel intracellular subtilisin protease (ISP) P355 derived from the genome of Planococcus halocryophilus Or1 was characterized. P355 was found to be one of the most unstable enzymes but had a higher catalytic rate. Compared to other ISPs (T0099, T0034, and SC), P355 and T0034 showed higher catalytic constants, demonstrating the typical stability-activity trade-off observed in cold-adapted proteases.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Guillem Macip, Pol Garcia-Segura, Julia Mestres-Truyol, Bryan Saldivar-Espinoza, Gerard Pujadas, Santiago Garcia-Vallve
Summary: This review collected 1765 SARS-CoV-2 M-pro inhibitors and discussed their properties, measurement variations, and inhibitory effects on viral replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaoyang Chen, Xiabing Li, Yuhang Duan, Zhangxin Pei, Hao Liu, Weixiao Yin, Junbin Huang, Chaoxi Luo, Xiaolin Chen, Guotian Li, Kabin Xie, Tom Hsiang, Lu Zheng
Summary: In this study, we identified a secreted fungal subtilase, UvPr1a, from the rice false smut fungus Ustilaginoidea virens, which interferes with rice immunity by degrading the rice protein OsSGT1, thereby promoting infection. This discovery provides tools for introducing resistance to rice false smut and deepens our understanding of plant-pathogen interactions.
Article
Biochemistry & Molecular Biology
Mizuki Tokonami, Akari Horiguchi, Naoki Ueshima, Teisuke Takita, Toru Takahashi, Kosaku Nishimura, Kiyoshi Yasukawa
Summary: In this study, ficin and a new serine protease FSP were purified from Ficus carica cultivar Masui Dauphine fruit. FSP was identified as a subtilisin-like protease and exists as a dimer. The optimal pH and temperature for FSP activity were found to be 7.5 and 50-60 degrees C, respectively.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Immunology
Rong Wang, Qingyan Li, Fangyan Liu, Xiaoqun Dang, Quan Sun, Xiaotian Sheng, Mingyu Hu, Jialing Bao, Jie Chen, Guoqing Pan, Zeyang Zhou
Summary: Microsporidia infect host cells through germination, and NbSLP1 protein plays a significant role in this process. The study found that the signal peptide of NbSLP1 affects the activation of the protease, and intramolecular proteolysis is essential for NbSLP1 maturation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Zenichi Ikeda, Taku Kamei, Yusuke Sasaki, Matthew Reynolds, Nozomu Sakai, Masato Yoshikawa, Michiko Tawada, Nao Morishita, Douglas R. Dougan, Chien-Hung Chen, Irena Levin, Hua Zou, Masako Kuno, Naoto Arimura, Yusuke Kikukawa, Mitsuyo Kondo, Kimio Tohyama, Kenjiro Sato
Summary: We explored a novel series of non-amidine-based C1s inhibitors. By replacing isoquinoline with 1-aminophthalazine, we enhanced the C1s inhibitory activity while maintaining good selectivity against other serine proteases. Through structure-based optimization around the S2 and S3 sites and improving membrane permeability, we identified (R)-8 as a potent, selective, orally available, and brain-penetrable C1s inhibitor, which effectively blocked the classical complement pathway.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Fabian Falkenberg, Jade Rahba, David Fischer, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: The study characterized a high-alkaline subtilisin and found that it retains good activity under high salt concentrations and high temperatures. It also demonstrated high oxidative stability to hydrogen peroxide, making it a promising candidate for biotechnological applications.
Article
Chemistry, Medicinal
Lucile Brier, Haitham Hassan, Xavier Hanoulle, Valerie Landry, Danai Moschidi, Lowiese Desmarets, Yves Rouille, Julie Dumont, Adrien Herledan, Sandrine Warenghem, Catherine Piveteau, Paul Carr, Sarah Ikherbane, Francois-Xavier Cantrelle, Elian Dupre, Jean Dubuisson, Sandrine Belouzard, Florence Leroux, Benoit Deprez, Julie Charton
Summary: Since the end of 2019, the global outbreak of the coronavirus SARS-CoV-2 has caused a significant increase in infections and deaths worldwide. Researchers have identified two proteases, 3CLpro and papain-like protease, encoded by the virus that play a crucial role in the virus lifecycle. Targeting the 3CLpro, which is highly conserved within this viral family, a screening of over 89,000 small molecules led to the discovery of a potent inhibitor of the SARS-CoV-2 3CLpro. The mechanism of inhibition, interaction with the protease, specificity against host proteases, and promising antiviral properties in cells are reported.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yasel Guerra, Vinicio Armijos-Jaramillo, Tirso Pons, Eduardo Tejera, Colin Berry
Summary: The Kunitz-Soybean Trypsin Inhibitor (Kunitz-STI) family is a large family of proteins with most of its members being protease inhibitors. This study analyzed the crystallographic structures of Kunitz-STI inhibitors to characterize the structural features and locations of serine protease-binding loops. The findings suggest a relationship between the conformation of these loops, the inhibition mechanism, and the plant source of the inhibitors. This research challenges the perception that all Kunitz-STI inhibitors are canonical and will aid in the identification and design of multifunctional proteins.
Review
Physiology
Lisse Decraecker, Guy Boeckxstaens, Alexandre Denadai-Souza
Summary: Serine proteases play a crucial role in the development of abdominal pain in irritable bowel syndrome (IBS), and protease inhibitors are emerging as a potential therapeutic tool. However, the identity of dysregulated serine proteases released by the rectal mucosa of IBS patients remains poorly understood, highlighting a knowledge gap that needs to be addressed with advanced techniques.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Immunology
Kangchen Li, Qianting Ji, Shibo Jiang, Naru Zhang
Summary: This review discusses the pathogenesis of ZIKV infection and summarizes the progress in the development of therapeutics against ZIKV infection, providing guidance for the development of effective therapeutics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Pravin Muthu, Stefan Lutz
Article
Biochemistry & Molecular Biology
Pinar Iyidogan, Stefan Lutz
Article
Biochemistry & Molecular Biology
Stephan Reitinger, Ying Yu, Jacqueline Wicki, Martin Ludwiczek, Igor D'Angelo, Simon Baturin, Mark Okon, Natalie C. J. Strynadka, Stefan Lutz, Stephen G. Withers, Lawrence P. McIntosh
Article
Chemistry, Medicinal
Yongfeng Li, Priti B. Soni, Lingfeng Liu, Xiao Zhang, Dennis C. Liotta, Stefan Lutz
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2010)
Editorial Material
Biochemistry & Molecular Biology
Romas Kazlauskas, Stefan Lutz
CURRENT OPINION IN CHEMICAL BIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Joseph A. Laszlo, Ying Yu, Stefan Lutz, David L. Compton
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2011)
Article
Biochemical Research Methods
Elsie M. Williams, Se Min Jung, Jennifer L. Coffman, Stefan Lutz
ACS SYNTHETIC BIOLOGY
(2018)
Article
Biochemical Research Methods
Matthew C. Jenkins, Stefan Lutz
Summary: The study demonstrates the successful concurrent surface functionalization and internal packaging of encapsulins from Thermotoga maritima to create a catalytically competent two-enzyme metabolon, highlighting the engineerability of encapsulins as flexible scaffolds for biocatalytic applications. These engineered encapsulins functioned at speeds equivalent to those of the two enzymes freely dispersed in solution, emphasizing their potential in biotechnology and synthetic biology.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Monica L. Gerth, Stefan Lutz
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2007)
Article
Chemistry, Multidisciplinary
Z Qian, S Lutz
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2005)
Article
Biochemistry & Molecular Biology
PTR Rajagopalan, S Lutz, SJ Benkovic