Article
Microbiology
Wenzhu Guo, Dandan Liu, Jingen Li, Wenliang Sun, Tao Sun, Xingji Wang, Kefen Wang, Qian Liu, Chaoguang Tian
Summary: The transglycosylation activity of enzymes during starch hydrolysis is a key concern in the starch-hydrolysis industry. In this study, two key alpha-glucosidases responsible for producing non-fermentable sugars were identified, and overexpression of a specific domain significantly increased glucoamylase activity while reducing transglycosylation activity and product formation. These findings have important implications for reducing costs and adding value to starch biorefinery products.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Passakorn Teerapongpisan, Virayu Suthiphasilp, Pakit Kumboonma, Tharakorn Maneerat, Thidarat Duangyod, Rawiwan Charoensup, Raymond J. Andersen, Surat Laphookhieo
Summary: The first phytochemical investigation of the twigs of Phaeanthus lucidus Oliv. led to the discovery of four previously undescribed alkaloids, including two aporphine dimers, phaeanthuslucidines A and B, a hybrid of aristolactam-aporphine, phaeanthuslucidine C, and a C-N linked aporphine dimer, phaeanthuslucidine D, along with two known compounds. Their structures were determined through extensive spectroscopic analysis and comparison with previous reports. Chiral HPLC analysis of phaeanthuslucidines A-C and bidebiline E yielded the (R-a) and (S-a) atropisomers, whose absolute configurations were determined by ECD calculations. Phaeanthuslucidines A and B, bidebiline E, and lanuginosine exhibited alpha-glucosidase inhibitory activities with IC50 values ranging from 6.7 to 29.2 μM. Molecular docking simulations of the active compounds' inhibition of alpha-glucosidase were also studied.
Article
Food Science & Technology
Vittorio Silano, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Claude Lambre, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Lieve Herman, Jaime Aguilera, Magdalena Andryszkiewicz, Davide Arcella, Yi Liu, Elsa Nielsen, Karin Norby, Andrew Chesson
Summary: The food enzyme alpha-glucosidase, produced with non-genetically modified Aspergillus niger strain AE-TGU, is safe for use in various food processes with low risk of allergic sensitization. The estimated dietary exposure in European populations is within acceptable limits. Toxicity tests and studies indicate that the enzyme does not raise safety concerns under the intended conditions of use.
Article
Biochemistry & Molecular Biology
Tao Yang, Kailing Yang, Yu Zhang, Ruixi Zhou, Fuxin Zhang, Guanqun Zhan, Zengjun Guo
Summary: One new compound and 13 known compounds were isolated from Aspergillus niger, a plant endophytic fungus. Compound 1 has a simple structure and remarkable biological activity, making it a potential lead compound. Compounds 2 and 3 exhibited significant antioxidant activities, while compound 1 displayed remarkable inhibitory activity against alpha-glucosidase.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Claude Lambre, Jose Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Riviere, Inger-Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Boet Glandorf, Lieve Herman, Jaime Aguilera, Natalia Kovalkovicova, Yi Liu, Andrew Chesson
Summary: This article discusses the production and application of a food enzyme called glucan 1,4-alpha-glucosidase. However, due to the lack of data and reports, the safety of this food enzyme produced from an unknown strain of Aspergillus niger cannot be assessed.
Article
Chemistry, Multidisciplinary
Ngoc-Hong Nguyen, Nguyen-Minh-An Tran, Thuc-Huy Duong, Giau Van Vo
Summary: In this study, we found eight flavonoid compounds from Bouea macrophylla that showed promising antidiabetic properties. Among them, 8-bromoquercetin exhibited the highest inhibition activity, being 37 times more effective than the standard drug acarbose. These compounds have the potential to be developed as potential drugs.
Article
Food Science & Technology
Yucong Zou, Xiaofeng Li, Xuan Xin, Haixia Xu, Lan Mo, Yigang Yu, Guanglei Zhao
Summary: A new Aspergillus niger whole-cell catalyst was cultured for the hydrolysis of hesperidin to produce hesperetin-7-O-glucoside and hesperetin. The choice of inducers affected the catalytic activity and product composition of the cells. Transcriptome analysis revealed different gene expressions in response to different inducers. Several genes related to enzyme activities were identified. qRT-PCR analysis confirmed the reliability of the transcriptome sequencing results.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Wenzhu Guo, Jianhua Yang, Tianchen Huang, Dandan Liu, Qian Liu, Jingen Li, Wenliang Sun, Xingji Wang, Leilei Zhu, Chaoguang Tian
Summary: This study identified key enzymes involved in the starch saccharification process, including glucoamylase, alpha-amylase, and acid alpha-amylase, out of 29 glycoside hydrolases from the 6-day fermentation products of A. niger O1. Synergistic effects were observed among the components of glucoamylases cocktail, with an increase in alpha-amylase leading to improved efficiency of starch saccharification. Overexpression of acid alpha-amylase in strain O1 resulted in an increase in total glucoamylase activity.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Chemistry, Multidisciplinary
Mahendra Gowdru Srinivasa, Jagdish Gopal Paithankar, Sumit Rao Saheb Birangal, Aravinda Pai, Vasudev Pai, Shridhar N. Deshpande, B. C. Revanasiddappa
Summary: Hybrid molecules containing multiple biologically active functional groups have a greater affinity and efficiency compared to standard medicinal compounds. In this study, a combination of thiazolidinediones and 1,3,4-oxadiazoles was investigated for its potential enhancement of alpha-amylase and alpha-glucosidase inhibition activity. Novel derivatives were synthesized and characterized, and in silico and in vitro assays were performed to evaluate their inhibitory effects. The compounds showed promising inhibitory activity and could be further developed as a new class of antidiabetic agents.
Article
Microbiology
Sandra Garrigues, Roland S. Kun, Mao Peng, Birgit S. Gruben, Isabelle Benoit Gelber, Miia Makela, Ronald P. de Vries
Summary: The study compared the transcriptomic responses of Aspergillus niger to growth on liquid and solid cultures containing sugar beet pulp as a carbon source. It was found that the culture condition highly affected the transcriptomic response of A. niger, with the response in a liquid environment unable to fully explain the behavior of the fungus in a solid environment. This suggests that submerged cultures may introduce variations in fungal physiology during growth on plant biomass.
MICROBIOLOGY SPECTRUM
(2021)
Article
Plant Sciences
Wei Shen, Xiaojing Huang, Kai Wang
Summary: A strain was isolated from Hangzhou Bay's sea sediment and cultured using rice bran through solid fermentation. Ethyl acetate was used to extract the fermented material, from which 8 butenolide derivatives (1-8) were isolated and their structures were determined through spectroscopic techniques. Compound 1 was identified as a new compound and named 4-demethyl ester aspernolide N, while the previously misassigned derivative was revised. Compounds 2-6 showed better inhibitory effects on alpha-glucosidase compared to the positive control acabose.
RECORDS OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Shoaib Khan, Shahid Iqbal, Muhammad Taha, Rafaqat Hussain, Fazal Rahim, Mazloom Shah, Nasser S. Awwad, Hala A. Ibrahium, Mohammed Issa Alahmdi, Ayed A. Dera, Hayat Ullah, Ali Bahadur, Samar O. Aljazzar, Eslam B. Elkaeed, Muhammad Rauf
Summary: The clinical significance of benzimidazole-containing drugs has increased, and research interest in these moieties is strong due to their diverse biological features. This study aimed to synthesize a new series of benzimidazole analogs and evaluate their inhibitory activity against alpha-amylase and alpha-glucosidase. Most of the derivatives showed promising activity, with analog 2 displaying the best inhibitory activity. The structure-activity relationship (SAR) was determined by examining the substitution effects, and molecular docking provided insights into the interactions between the active substances and the targeted enzymes.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wenwen He, Yanchao Xu, Dan Wu, Dongyang Wang, Hai Gao, Liping Wang, Weiming Zhu
Summary: Three new alkaloids and four previously reported compounds were identified from the fermentation broth of the endophytic fungus associated with Garcinia muldflora. Some of these compounds showed inhibitory activity against alpha-glucosidase, with structural insights provided by spectroscopic analysis and quantum-mechanical energies calculations.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Hua Zhu, Xin Zhong
Summary: In this study, six flavonoid derivatives were synthesized and tested for their anti-alpha-glucosidase activities. The results showed that all derivatives exhibited excellent inhibitory effects on alpha-glucosidase, with derivative four displaying the highest activity. Further analysis of the structure-activity relationship, inhibitory mechanism, and molecular docking revealed that the bromine group was the most beneficial group for anti-alpha-glucosidase activity.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Chun-Mei Hu, Ying-Ying Zheng, Ai-Tian Lin, Xin Zhang, Xiao-Zheng Wu, Jing Lin, Xue-Tao Xu, Zhuang Xiong
Summary: A series of derivatives were synthesized and evaluated for their inhibitory activities on α-glucosidase. Compound 4j exhibited the highest inhibitory activity. Spectroscopy and docking results revealed the binding mechanism and mode of action of compound 4j on α-glucosidase.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
