Article
Dermatology
Wei Shang, Alvin Yong Quan Tan, Maurice A. M. van Steensel, Xinhong Lim
Summary: Balancing stem cell proliferation and differentiation is crucial for tissue maintenance and growth. Dysregulation of stem cell regulation, such as Wnt signaling, may lead to abnormal fate determination of stem cells in the hair follicle junction zone (JZ), resulting in cystic structures resembling human acne. Modulating Wnt signaling in JZ stem cells in mouse models confirmed the involvement of Wnt signaling in the formation of JZ cysts and sebaceous gland atrophy. Additionally, all-trans retinoic acid treatment and Hedgehog signaling inhibition showed promising therapeutic effects on these cysts.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Prem Swaroop Yadav, Shuhao Feng, Qian Cong, Hanjun Kim, Yuchen Liu, Yingzi Yang
Summary: Job syndrome is a rare genetic disorder characterized by immune dysfunction and skeletal developmental abnormalities. Current treatment options are limited, but enhancing Wnt/β-catenin signaling could potentially reduce bone symptoms in patients with Job syndrome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Pathirennehelage Chandrasekera, Mark Perfetto, Congyu Lu, Minghui Zhuo, Harinath Bahudhanapati, Jiejing Li, Wei-Chih Chen, Pallavi Kulkarni, Laura Christian, Jun Liu, Yvette Y. Yien, Chundong Yu, Shuo Wei
Summary: This study demonstrates that ADAM9 in mammals can cleave ephrin-B1 and ephrin-B2, promoting neural crest induction. The study also found that ADAM9 expression is elevated in colorectal cancer (CRC) tissues and knocking down ADAM9 inhibits the migration and invasion of CRC cells, mediated by reducing Akt kinase activity. Additionally, the study reveals differential effects of ADAM9 knockdown on Wnt signaling and mTOR signaling in different CRC cell lines.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Multidisciplinary Sciences
Abhishek Sinha, Vinson B. Fan, Aravinda-Bharathi Ramakrishnan, Nicole Engelhardt, Jennifer Kennell, Ken M. Cadigan
Summary: SOX9 promotes turnover of β-catenin independently of the destruction complex and proteasome in mammalian cell culture. This requires the activation of MAML2, which acts as a coactivator for SOX9 in antagonizing the Wnt/β-catenin pathway. This newly identified pathway provides a parallel mechanism for restricting Wnt/β-catenin signaling.
Article
Pharmacology & Pharmacy
Xiujuan Zhu, Huanyao Gao, Sisi Qin, Duan Liu, Junmei Cairns, Yayun Gu, Jia Yu, Richard M. M. Weinshilboum, Liewei Wang
Summary: This study has revealed a novel mechanism for regulation of the expression of cholesterol-metabolizing CYPs, particularly CYP1B1, by TSPYL1 via Wnt/beta-catenin signaling.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shuangyi Cai, Thomas Hu, Mythreye Venkatesan, Mayar Allam, Frank Schneider, Suresh S. Ramalingam, Shi-Yong Sun, Ahmet F. Coskun
Summary: Protein-protein interaction networks are altered in multi-gene dysregulations in many disorders. Image-based protein multiplexing sheds light on signaling pathways to dissect cell-to-cell heterogeneity, previously masked by the bulk assays. RapMIF is a rapid multiplexed immunofluorescence method that can measure the spatial protein maps of up to 25 proteins, providing combinatorial signaling states and enabling precision therapy design.
Article
Medicine, Research & Experimental
Hoda E. Mohamad, Dina M. Abo-Elmatty, Nehal S. Wahba, Mohamed A. Shaheen, Rowan T. Sakr, Alaa S. Wahba
Summary: The current study aimed to investigate the neurotoxic potential of DOX in inducing AD-like pathology and the efficacy of infliximab (IFX) and 2-mercaptoethane sulfonate sodium (MESNA) in attenuating DOX-induced neurotoxicity. The results showed that both IFX and MESNA significantly mitigated DOX-induced brain injury, neuroinflammation, and apoptosis, with the best improvement observed with their combined administration.
Article
Biochemistry & Molecular Biology
Kuangen Zhang, Rui Wu, Fang Mei, Yuhe Zhou, Lin He, Yanhua Liu, Xuyang Zhao, Jiangfeng You, Beiying Liu, Qingyang Meng, Fei Pei
Summary: LASS2 acts as a tumor-suppressor gene in prostate cancer by promoting beta-catenin degradation through physical interaction with specific proteins, and phosphorylation of LASS2 at a specific residue plays a crucial role in inhibiting cancer cell growth and metastasis.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Zheng Qin, Yupei Li, Jiameng Li, Luojia Jiang, Zhuyun Zhang, Kaixi Chang, Qinbo Yang, Shanshan Chen, Ruoxi Liao, Baihai Su
Summary: This study found that exosomal STAT1 derived from HP-treated HUVECs can promote VSMC calcification. Activation of the Wnt/beta-catenin pathway may be a potential mechanism of exosome-promoted VSMC calcification.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Zeliha Guney, Sivge Kurgan, Canan Onder, Mahmure Ayse Tayman, Oemer Gunhan, Alpdogan Kantarci, Muhittin Abdulkadir Serdar, Meral Gunhan
Summary: This study aimed to evaluate the activity of the Wnt/beta-catenin signaling pathway in gingival samples obtained from patients with periodontitis. The results showed that periodontal inflammation disrupts the Wnt/beta-catenin signaling pathway, leading to tissue inflammation and changes in morphology. This finding may shed light on the pathogenesis of periodontal disease.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Biochemistry & Molecular Biology
Rocio Jimenez-Guerrero, Alejandro Belmonte-Fernandez, M. Luz Flores, Monica Gonzalez-Moreno, Begona Perez-Valderrama, Francisco Romero, Miguel A. Japon, Carmen Saez
Summary: This study reveals the role of the Wnt/β-catenin pathway in paclitaxel resistance in bladder cancer cells with CSC-like properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Michael Latour, Nam-Gu Her, Santosh Kesari, Elmar Nurmemmedov
Summary: The WNT signaling pathway plays a crucial role in glioblastoma genesis and therapy resistance, presenting a therapeutic challenge. Current targeted therapies under clinical investigation may offer new treatment options.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Oncology
Jason K. Sa, Yeup Yoon, Misuk Kim, Yeonghwan Kim, Hee Jin Cho, Jin-Ku Lee, Gi-Soo Kim, Suji Han, Woon Jin Kim, Yong Jae Shin, Kyeung Min Joo, Patrick J. Paddison, Tohru Ishitani, Jeongwu Lee, Do-Hyun Nam
Article
Plant Sciences
Kok Hoong Leong, Mohamad Azrul Mahdzir, Mohd Fadzli Md Din, Khalijah Awang, Yuka Tanaka, Kasem Kulkeaw, Tohru Ishitani, Daisuke Sugiyama
Article
Neurosciences
Yuto Ueda, Yuki Shimizu, Nobuyuki Shimizu, Tohru Ishitani, Toshio Ohshima
JOURNAL OF COMPARATIVE NEUROLOGY
(2018)
Article
Neurosciences
Masashi Watanave, Chiaki Hoshino, Ayumu Konno, Yumi Fukuzaki, Yasunori Matsuzaki, Tohru Ishitani, Hirokazu Hirai
NEUROBIOLOGY OF DISEASE
(2019)
Article
Cell Biology
Kota Abe, Atsuko Shimada, Sayaka Tayama, Hotaka Nishikawa, Takuya Kaneko, Sachiko Tsuda, Akari Karaiwa, Takaaki Matsui, Tohru Ishitani, Hiroyuki Takeda
Article
Multidisciplinary Sciences
Yuki Akieda, Shohei Ogamino, Hironobu Furuie, Shizuka Ishitani, Ryutaro Akiyoshi, Jumpei Nogami, Takamasa Masuda, Nobuyuki Shimizu, Yasuyuki Ohkawa, Tohru Ishitani
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Masayuki Oginuma, Yukiko Harima, Oscar A. Tarazona, Margarete Diaz-Cuadros, Arthur Michaut, Tohru Ishitani, Fengzhu Xiong, Olivier Pourquie
Article
Biochemistry & Molecular Biology
Yusuke Mori, Naoka Shiratsuchi, Nanami Sato, Azusa Chaya, Nobuyuki Tanimura, Susumu Ishikawa, Mugihiko Kato, Ikumi Kameda, Shunsuke Kon, Yukinari Haraoka, Tohru Ishitani, Yasuyuki Fujita
Summary: This study found that reactive oxygen species (ROS) are elevated in the surrounding epithelial layers during cell extrusion, and reducing ROS levels inhibits the extrusion process. Additionally, extruding cells release ATP, which promotes ROS levels and cell extrusion. The extracellular ATP and ROS pathways also regulate the movement of surrounding cells towards the extruding cells. These findings highlight the important role of extracellular ATP in cell extrusion.
Article
Multidisciplinary Sciences
Yukinari Haraoka, Yuki Akieda, Yuri Nagai, Chihiro Mogi, Tohru Ishitani
Summary: The study demonstrates that oncogenic cells can be eliminated through oncogene-induced senescence, but this behavior can be altered by a p53 mutation, leading to promotion rather than suppression of tumorigenesis. The findings provide important insights into the initial step of tumor formation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Hisato Suzuki, Kana Aoki, Kenji Kurosawa, Kazuo Imagawa, Tatsuyuki Ohto, Mamiko Yamada, Toshiki Takenouchi, Kenjiro Kosaki, Tohru Ishitani
Summary: CTR9 gene mutations are associated with macrocephaly, motor delay, and intellectual disability. The mutant proteins showed stronger affinity to PAF1 protein, and knockout of ctr9 gene in zebrafish resulted in motor defects and enlargement of the telencephalon. Rescue experiments failed to restore normal function, and overexpression of human CTR9 mutant mRNA caused enlargement of telencephalon in zebrafish larvae.
HUMAN MOLECULAR GENETICS
(2022)
Article
Multidisciplinary Sciences
Masayuki Oginuma, Moana Nishida, Tomomi Ohmura-Adachi, Kota Abe, Shohei Ogamino, Chihiro Mogi, Hideaki Matsui, Tohru Ishitani
Summary: Researchers have developed a new CRISPR/Cas9 method for rapid generation of knockout and transgenic African turquoise killifish. They have also successfully developed a method for knock-in of reporter genes without crossing. These advances will accelerate aging and developmental studies using the fish model.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Akira Kikuchi, Junichi Takagi, Shinji Takada, Tohru Ishitani, Yasuhiro Minami
Summary: Wnt2022 was an in-person meeting held in Awaji Yumebutai International Conference Center, Hyogo Prefecture, Japan, from November 15th to 19th, 2022, marking the first physical gathering in the past three years. The conference aimed to commemorate the 40th anniversary of Wnt research and discuss the future direction of this field, considering its crucial roles in various physiological and pathological processes. Despite travel and administrative restrictions due to COVID-19, the meeting successfully brought together 148 researchers from 21 countries, enabling face-to-face discussions.
Article
Cell Biology
Tohru Ishitani
Summary: Morphogen gradients play a crucial role in tissue patterning. Cadherin-mediated cell competition is involved in ensuring the formation of robust morphogen gradients. This process eliminates unfit cells and corrects noisy gradients, promoting reproducible patterning.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Hematology
Kasem Kulkeaw, Tomoko Inoue, Tohru Ishitani, Yoichi Nakanishi, Leonard I. Zon, Daisuke Sugiyama
BRITISH JOURNAL OF HAEMATOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Seung-Ju Cho, Bok-Sik Cha, Ok-Seon Kwon, Jisun Lim, Dong-Myung Shin, Dong Wook Han, Tohru Ishitani, Eek-hoon Jho, Albert J. Fornace, Hyuk-Jin Cha
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2017)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)
