Article
Biochemistry & Molecular Biology
Xiaohong Zhang, Zhixing Liu, Mengdi Li, Ziyang Zhang, Xiaochen Wen, Daochuan Zhang, Hong Yin
Summary: The transcription factor Scalloped (Sd) regulates cell proliferation and organ growth in the Hippo pathway. The research identified and characterized LmSd, showing its importance in wing formation and development by controlling cell proliferation and inhibiting apoptosis. The study found that silencing of LmSd resulted in deformed wings and abnormal wing pad closure during molting.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Immunology
Woojong Lee, M. Suresh
Summary: This review focuses on recent insights into DC cross-presentation and the impact of clinically relevant vaccine adjuvants on DC cross-presentation efficiency. Understanding these mechanisms is crucial for the rational design of vaccines that elicit balanced antibody- and T cell-based immunity against infectious diseases and cancer.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Monika Priyadarshini, Julie Zhouli Ni, Amhed M. Vargas-Velazquez, Sam Guoping Gu, Christian Frokjaer-Jensen
Summary: This work presents a piRNA-mediated interference method for multiplexed gene silencing in Caenorhabditis elegans. The piRNAi method is more efficient than RNAi and can be multiplexed. It can induce transgenerational epigenetic silencing and has the potential for scalability.
Article
Entomology
Lu Gao, Yanli Wang, Mureed Abbas, Tingting Zhang, Enbo Ma, Shuping Xing, Kun Yan Zhu, Jianzhen Zhang
Summary: The study identified an LmR2D2 gene in Locusta migratoria, encoding a protein with two dsRNA-binding domains. LmR2D2 plays an important role in the siRNA pathway, and its expression levels are stable during development in Locusta migratoria, with higher levels in the testis and ovary. Silencing LmR2D2 led to reduced RNAi efficiency against a marker gene, supporting its essential role in siRNA binding in vitro.
Article
Physiology
Muhammad Zaryab Khalid, Zhipeng Sun, Jing Zhang, Shijie Zhang, Guohua Zhong
Summary: Cyromazine has been found to decrease the number of germ cells in livestock and household insect pests by affecting the ecdysone signaling pathway. This finding is important for understanding the mechanism of action of cyromazine.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agronomy
Chunni Zhang, Taoling Zhou, Yao Li, Wu Dai, Shaokai Du
Summary: In this study, the overexpression of CYP6FV21 gene was found in the clothianidin-resistant strain of B. odoriphaga. Knockdown of CYP6FV21 increased the susceptibility of larvae to clothianidin. Thus, the overexpression of the CYP6FV21 gene is the molecular basis for clothianidin resistance.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ni Wang, Min Chen, Ying Zhou, Wen-Wu Zhou, Zeng-Rong Zhu
Summary: This study systematically characterized five core genes involved in miRNA biogenesis and found that they play essential roles in the development and reproduction of the Nilaparvata lugens agricultural pest.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Hanbang Zhang, Juliana Veira, Sarah Twitty Bauer, Christopher Yip, Upinder Singh
Summary: This study characterizes, for the first time, the components of the RNA-induced silencing complex (RISC) in Entamoeba, revealing multiple functional protein components and novel nuclear assembly protein-interacting partners. The research further enhances our understanding of the molecular regulatory mechanisms of RNAi in this parasite.
Article
Agricultural Engineering
Ran Xu, Litao Zhang, Wenjie Yu, Jianguo Liu
Summary: The study used transcriptomic data and a transformation system to downregulate the key enzyme alpha-1,6-mannosyltransferase (HpOCH1) in Haematococcus pluvialis cells, resulting in thinner cell walls and simplified astaxanthin extraction. The downregulation of HpOCH1 led to decreased cell wall organization and protection, but did not affect astaxanthin synthesis.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Matthew E. Griffin, Alexander W. Sorum, Gregory M. Miller, William A. Goddard, Linda C. Hsieh-Wilson
Summary: Heparan sulfate glycosaminoglycans (HS GAGs) regulate Ang-Tie signaling by interacting directly with Ang ligands and Tie1 receptors, enhancing endothelial survival signaling and maintaining Tie1 stability within the mature vasculature. Loss of HS-Tie1 binding leads to decreased Tie protein levels, pathway suppression, and aberrant retinal vascularization.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yanxin Ren, Xueyu Li, Zhonghui Tian, Yan Xu, Ruilin Zhang, Yang Li
Summary: Zebrafish possess evolutionarily conserved innate and adaptive immunity, making them a valuable model organism for studying infection and immunity. This study evaluated the potential of zebrafish to study antiviral RNAi, showing that virus-derived small interfering RNAs are produced after infection with SINVs and NoVs. The slicing activity of human Ago2 can inhibit RNA virus accumulation when expressed in larval zebrafish, suggesting zebrafish as a unique model for studying the antiviral RNAi pathway.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jiajia Zhao, Shuo Yan, Mingshan Li, Lina Sun, Min Dong, Meizhen Yin, Jie Shen, Zhangwu Zhao
Summary: RNA biopesticides are seen as the third revolution in pesticides history due to their precision, efficiency, and eco-friendliness. Through a star polycation nanocarrier-based delivery system, high RNA interference efficiency of npfr was achieved. A highly efficient bacteria-based expression system was used to produce large amounts of dsRNA segments targeting npfr and ampk simultaneously, which were then complexed with nanocarriers to develop a dual-target RNA pesticide. It significantly inhibits larval feeding, growth, and development, outperforming the widely-used azadirachtin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agronomy
Liqiong Yan, Hui Du, Ye Li, Xue Li, Lili Sun, Chuanwang Cao
Summary: In this study, five genes in the insulin signaling pathway were identified and characterized in Hyphantria cunea. The expression profiles of these genes in different developmental stages and tissues were investigated. RNA interference experiments revealed that these genes play important roles in the growth, development, and molting process of H. cunea. The findings provide a valuable basis for developing RNAi-based pest control strategies and understanding the mechanism of H. cunea outbreaks.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Bi-Yue Ding, Xiu-Cheng Xie, Feng Shang, Guy Smagghe, Jin-Zhi Niu, Jin-Jun Wang
Summary: The study on carotenoid biosynthetic genes in pea aphids shows that different genes have specific functions in the synthesis process, and silencing some of these genes can affect the overall carotenoid levels.
Article
Biotechnology & Applied Microbiology
Defne A. Amado, Julianne M. Rieders, Fortunay Diatta, Pilar Hernandez-Con, Adina Singer, Jordan T. Mak, Junxian Zhang, Eric Lancaster, Beverly L. Davidson, Alice S. Chen-Plotkin
Article
Cell & Tissue Engineering
Jean Ann Maguire, Alyssa L. Gagne, Pedro Gonzalez-Alegre, Beverly L. Davidson, Vikram Shakkottai, Paul Gadue, Deborah L. French
STEM CELL RESEARCH
(2019)
Editorial Material
Biotechnology & Applied Microbiology
Beverly L. Davidson
NATURE BIOTECHNOLOGY
(2019)
Review
Biotechnology & Applied Microbiology
Defne A. Amado, Beverly L. Davidson
Summary: ALS has historically been challenging for gene therapy due to a lack of therapeutic targets and difficulty accessing the brain and spinal cord. Recent advances in disease mechanism understanding, ALS genetics, and CNS targeting, along with progress in gene delivery and editing techniques, have opened new therapeutic possibilities. Gene therapy clinical trials are underway for ALS patients with various genetic mutations, showing potential for disease-modifying treatment.
Review
Biotechnology & Applied Microbiology
Jill A. Morris, Chris H. Boshoff, Nina F. Schor, Ling M. Wong, Guangping Gao, Beverly L. Davidson
Summary: NINDS organized a workshop on gene-targeted therapies for CNS disorders, bringing together experts from various fields to identify challenges and propose solutions. Prior to the workshop, key obstacles and research gaps were identified through pre-meeting teleconferences, allowing for problem-solving discussions and strategy development at the workshop.
Letter
Biotechnology & Applied Microbiology
David Leib, Yong Hong Chen, Alex Mas Monteys, Beverly L. Davision
Correction
Multidisciplinary Sciences
Alex Mas Monteys, Amiel A. Hundley, Paul T. Ranum, Luis Tecedor, Amy Muehlmatt, Euyn Lim, Dmitriy Lukashev, Rajeev Sivasankaran, Beverly L. Davidson
Review
Biotechnology & Applied Microbiology
Beverly L. Davidson, Guangping Gao, Elizabeth Berry-Kravis, Allison M. Bradbury, Carsten Bonnemann, Joseph D. Buxbaum, Gavin R. Corcoran, Steven J. Gray, Heather Gray-Edwards, Robin J. Kleiman, Adam J. Shaywitz, Dan Wang, Huda Y. Zoghbi, Terence R. Flotte, Sitra Tauscher-Wisniewski, Cynthia J. Tifft, Mustafa Sahin
Summary: The review summarizes the discussions and presentations of a workshop sponsored by the National Institute of Mental Health, focusing on gene-based therapeutics for rare genetic neurodevelopmental psychiatric disorders. It highlights the potential of precision medicine approaches in treating these disorders.
Article
Medicine, Research & Experimental
Ellie M. Carrell, Megan S. Keiser, Ashley B. Robbins, Beverly L. Davidson
Summary: The study shows that targeting ATXN1 through RNA interference and overexpression of ATXN1L can prevent and reverse disease phenotypes in SCA1 mice. Combination of these therapeutic approaches leads to more effective SCA1 therapy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Sophie St-Cyr, Daniel D. Child, Emilie Giaime, Alicia R. Smith, Christine J. Pascua, Seung Hahm, Eddine Saiah, Beverly L. Davidson
Summary: A novel small molecule mTORC1 pathway activator, NV-5297, has been found to improve disease phenotypes in a mouse model of Huntington's Disease, including increased striatal volume, improved motor learning, and heart contractility.
Article
Biotechnology & Applied Microbiology
Bryan P. Simpson, Carolyn M. Yrigollen, Aleksandar Izda, Beverly L. Davidson
Summary: Using polymerase-free, targeted long-read nanopore sequencing, we evaluated the editing effects of single and dual gRNA AAV-CRISPR on the human ATXN2 gene in transgenic mouse models of SCA2. The results showed an editing rate of 10%-25% and identified AAV integration, large deletions, and transgene rearrangements as unexpected outcomes. PCR-based nanopore sequencing, in contrast, showed a bias for partial AAV fragments and inverted terminal repeats and failed to detect full-length AAV. This study provides a comprehensive understanding of the outcome spectrum of CRISPR editing in the mouse brain after AAV gene transfer using an unbiased long-read sequencing approach.
Letter
Biotechnology & Applied Microbiology
Paul T. Ranum, Luis Tecedor, Megan S. Keiser, Yong Hong Chen, David E. Leib, Xueyuan Liu, Beverly L. Davidson
Article
Multidisciplinary Sciences
Alex Mas Monteys, Amiel A. Hundley, Paul T. Ranum, Luis Tecedor, Amy Muehlmatt, Euyn Lim, Dmitriy Lukashev, Rajeev Sivasankaran, Beverly L. Davidson
Summary: A universal switch element has been developed for precise control of gene replacement or editing machinery after exposure to small molecules. This switch system, called X-on, allows controlled protein expression by adjusting drug dose, protein stability, and redosing. It offers an opportunity to refine and tailor gene therapies in humans due to its oral bioavailability and safety of the drugs used.
Article
Medicine, Research & Experimental
Rebecca C. Ahrens-Nicklas, Luis Tecedor, Arron F. Hall, Elena Lysenko, Akiva S. Cohen, Beverly L. Davidson, Eric D. Marsh
Review
Neurosciences
Carolyn M. Yrigollen, Beverly L. Davidson
