Improving livestock for agriculture - technological progress from random transgenesis to precision genome editing heralds a new era

Generation of Transgenic Pigs by Cytoplasmic Injection of piggyBac Transposase-Based pmGENIE-3 Plasmids1

(2014) Zicong Li et al.   BIOLOGY OF REPRODUCTION

Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos1

(2014) Kristin M. Whitworth et al.   BIOLOGY OF REPRODUCTION

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

High-Level Recombinant Human Lysozyme Expressed in Milk of Transgenic Pigs Can Inhibit the Growth of Escherichia coli in the Duodenum and Influence Intestinal Morphology of Sucking Pigs

(2014) Dan Lu et al.   PLoS One

Efficient Generation of Myostatin (MSTN) Biallelic Mutations in Cattle Using Zinc Finger Nucleases

(2014) Junjie Luo et al.   PLoS One

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System

(2014) Wei Ni et al.   PLoS One

Generation of mastitis resistance in cows by targeting human lysozyme gene to  -casein locus using zinc-finger nucleases

(2014) X. Liu et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

What the French ban of Bt MON810 maize means for science-based risk assessment

(2013) Marcel Kuntz et al.   NATURE BIOTECHNOLOGY

Staying on target with CRISPR-Cas

(2013) Dana Carroll   NATURE BIOTECHNOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Comparative regulatory approaches for groups of new plant breeding techniques

(2013) Maria Lusser et al.   New Biotechnology

Consuming Transgenic Goats' Milk Containing the Antimicrobial Protein Lysozyme Helps Resolve Diarrhea in Young Pigs

(2013) Caitlin A. Cooper et al.   PLoS One

Handmade Cloned Transgenic Sheep Rich in Omega-3 Fatty Acids

(2013) Peng Zhang et al.   PLoS One

Efficient nonmeiotic allele introgression in livestock using custom endonucleases

(2013) W. Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer

(2013) Zhenfang Wu et al.   TRANSGENIC RESEARCH

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

The current state of GMO governance: Are we ready for GM animals?

(2012) Núria Vàzquez-Salat et al.   BIOTECHNOLOGY ADVANCES

Politics holds back animal engineers

(2012) Amy Maxmen   NATURE

Tiptoeing around transgenics

(2012) Emily Waltz   NATURE BIOTECHNOLOGY

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Primary Transgenic Bovine Cells and Their Rejuvenated Cloned Equivalents Show Transgene-Specific Epigenetic Differences

(2012) Lucia Alonso-González et al.   PLoS One

Identification of Short Hairpin RNA Targeting Foot-And-Mouth Disease Virus with Transgenic Bovine Fetal Epithelium Cells

(2012) Hongmei Wang et al.   PLoS One

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted microRNA expression in dairy cattle directs production of  -lactoglobulin-free, high-casein milk

(2012) A. Jabed et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of myostatin-targeted goat by nuclear transfer from cultured adult somatic cells

(2012) Zheng-Rong Zhou et al.   THERIOGENOLOGY

Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT)

(2012) Jannik Ejnar Jakobsen et al.   TRANSGENIC RESEARCH

Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle

(2011) Shengli Yu et al.   CELL RESEARCH

Site-specific recombinases: from tag-and-target- to tag-and-exchange-based genomic modifications

(2011) Soeren Turan et al.   FASEB JOURNAL

Production of transgenic calves expressing an shRNA targeting myostatin

(2011) K. Tessanne et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Transgenic salmon: a final leap to the grocery shelf?

(2011) Alison L Van Eenennaam et al.   NATURE BIOTECHNOLOGY

Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Cattle

(2011) Bin Yang et al.   PLoS One

Germline Transgenic Pigs by Sleeping Beauty Transposition in Porcine Zygotes and Targeted Integration in the Pig Genome

(2011) Wiebke Garrels et al.   PLoS One

Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter

(2011) Pimprapar Wongsrikeao et al.   Prion

Site-directed mutagenesis of the myostatin gene in ovine fetal myoblast cells in vitro

(2011) Yan-ling Zheng et al.   RESEARCH IN VETERINARY SCIENCE

Production of cloned transgenic cow expressing omega-3 fatty acids

(2011) Xia Wu et al.   TRANSGENIC RESEARCH

Lysozyme transgenic goats’ milk positively impacts intestinal cytokine expression and morphology

(2011) Caitlin A. Cooper et al.   TRANSGENIC RESEARCH

Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system

(2011) Daniel F. Carlson et al.   TRANSGENIC RESEARCH

Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts

(2010) Jannik E. Jakobsen et al.   MOLECULAR BIOLOGY REPORTS

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer

(2010) DengKe Pan et al.   Science China-Life Sciences

Pig transgenesis by Sleeping Beauty DNA transposition

(2010) Jannik E. Jakobsen et al.   TRANSGENIC RESEARCH

Production of recombinant human lysozyme in the milk of transgenic pigs

(2010) Jia Tong et al.   TRANSGENIC RESEARCH

High concentration of human lactoferrin in milk of rhLf-transgenic cows relieves signs of bovine experimental Staphylococcus chromogenes intramammary infection

(2010) Heli Simojoki et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Site-specific modification of the bovine genome using Cre recombinase-mediated gene targeting.

(2009) Catriona Graham et al.   Biotechnology Journal

Gene targeting from laboratory to livestock: Current status and emerging concepts

(2009) Götz Laible et al.   Biotechnology Journal

First US approval for a transgenic animal drug

(2009) Jim Kling   NATURE BIOTECHNOLOGY

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

Is there a risk from not using GE animals?

(2009) James D. Murray et al.   TRANSGENIC RESEARCH

The Importance of the Omega-6/Omega-3 Fatty Acid Ratio in Cardiovascular Disease and Other Chronic Diseases

(2008) Artemis P. Simopoulos   EXPERIMENTAL BIOLOGY AND MEDICINE

Expression and Characterization of Bioactive Recombinant Human α-Lactalbumin in the Milk of Transgenic Cloned Cows

(2008) J. Wang et al.   JOURNAL OF DAIRY SCIENCE

Generation of goats lacking prion protein

(2008) Guohua Yu et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Cattle Mammary Bioreactor Generated by a Novel Procedure of Transgenic Cloning for Large-Scale Production of Functional Human Lactoferrin

(2008) Penghua Yang et al.   PLoS One

Production of Prnp −/− goats by gene targeting in adult fibroblasts

(2008) Caihong Zhu et al.   TRANSGENIC RESEARCH

Expression of active recombinant human lactoferrin in the milk of transgenic goats

(2007) Jie Zhang et al.   PROTEIN EXPRESSION AND PURIFICATION