Review
Multidisciplinary Sciences
Wenping Wu, Jimin Zheng, Zongchao Jia
Summary: The mitochondrial uniporter is a Ca2+-selective ion-conducting channel in the inner mitochondrial membrane composed of MCU, MCUb, EMRE, MICU1, and MICU2. Recent structural studies have provided insights into the component structures and their spatial relationship, contributing to a better understanding of mitochondrial Ca2+ uptake and regulation. Further research in this area could lead to advancements in studying mitochondrial Ca2+ permeation and related diseases.
Article
Multidisciplinary Sciences
Macarena Rodriguez-Prados, Elena Berezhnaya, Maria Teresa Castromonte, Sergio L. Menezes-Filho, Melanie Paillard, Gyorgy Hajnoczky
Summary: Mitochondrial Ca2+ uptake is regulated by the mtCU complex, which consists of MCU, EMRE, and MICU1/2/3 subunits. MICU1 has been proposed to physically block the mtCU pore and regulate Ca2+ flux at different cytoplasmic [Ca2+]. We studied the mtCU in divalent-free conditions and found that MICU1 restricts Na+ influx, but some mtCU channels appear to lack MICU1-dependent gating in cells with high endogenous MICU1 expression. The rearrangement of mtCU and loss of MICU1 during mitoplast preparation might have affected previous studies in divalent-free conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Endocrinology & Metabolism
N. Vishnu, A. Hamilton, A. Bagge, A. Wernersson, E. Cowan, H. Barnard, Y. Sancak, K. J. Kamer, P. Spegel, M. Fex, A. Tengholm, V. K. Mootha, D. G. Nicholls, H. Mulder
Summary: This study demonstrated that deficiency of the accessory protein MICU2 leads to decreased mitochondrial Ca2+ uptake in insulin-secreting cells, resulting in disrupted insulin secretion and impaired mitochondrial function.
MOLECULAR METABOLISM
(2021)
Article
Cell Biology
Mayara S. Bertolini, Roberto Docampo
Summary: In this study, we generated TcMICU1/TcMICU2 double knockout cell lines using CRISPR/Cas9 genome editing and found that these knockout cell lines were unable to uptake calcium at low extramitochondrial calcium concentrations. Further investigation revealed that Mg2+ negatively regulates the mitochondrial calcium uptake mediated by MCUC.
Article
Critical Care Medicine
Xuexin Li, Bowen Sun, Jie Li, Wanlin Ye, Mingjuan Li, Fasheng Guan, Songlin Wu, Xuerong Luo, Jianguo Feng, Jing Jia, Xueru Liu, Tao Li, Li Liu
Summary: This study found that ICUAW is associated with impaired mitochondrial calcium uptake caused by a decreased MICU1:MCU protein ratio. Overexpression of MICU1 can improve this dysfunction and alleviate sepsis-induced skeletal muscle weakness and atrophy.
Article
Cell Biology
Emily Fernandez Garcia, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Anil K. Rustgi, Jason R. Pitarresi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin Foskett
Summary: Inhibition of MCU in oncogenic cell lines leads to an energetic crisis and reduced cell proliferation unless media is supplemented with nucleosides, pyruvate or a-KG.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Giorgia Pallafacchina, Sofia Zanin, Rosario Rizzuto
Summary: Advances in scientific research have led to increasing interest in the regulation and physiological role of mitochondrial Ca2+ signaling, with the discovery and characterization of genes and pathways involved in recent years. The identification of the mitochondrial channel components and their function over the past decade has provided a clearer understanding of the role of mitochondrial Ca2+ signals in cellular processes. Recent studies have highlighted the importance of the MCU complex in controlling muscle nutrition and metabolism.
Article
Biochemistry & Molecular Biology
Toshitaka Nakamura, Motoyuki Ogawa, Kazuki Kojima, Saki Takayanagi, Shunya Ishihara, Kazuki Hattori, Isao Naguro, Hidenori Ichijo
Summary: The study revealed that mitochondrial Ca2+ regulation and hyperpolarization play a key role in lipid peroxidation and ferroptosis under cold stress.
Article
Biology
Vivek Garg, Junji Suzuki, Ishan Paranjpe, Tiffany Unsulangi, Liron Boyman, Lorin S. Milescu, W. Jonathan Lederer, Yuriy Kirichok
Summary: The study reveals that the MICU subunits do not block the MCUcx pore in the absence or presence of extramitochondrial Ca2+, but instead potentiate MCUcx activity by modifying its gating properties to spend more time in the open state when extramitochondrial Ca2+ is elevated.
Article
Cardiac & Cardiovascular Systems
Celio Damacena de Angelis, Benney T. Endoni, Daniel Nuno, Kathryn Lamping, Johannes Ledolter, Olha M. Koval, Isabella M. Grumbach
Summary: This study investigated sex differences in vasoreactivity and the role of mitochondrial Ca2+ entry via the MCU in resistance arteries. Female mice exhibited reduced vasodilation compared to male mice in genetic models of endothelial MCU ablation. These differences were attributed to higher cytosolic Ca2+ transients and enhanced mitochondrial Ca2+ entry in female mice. Estradiol treatment in human aortic endothelial cells also increased cytosolic Ca2+ transients and mitochondrial Ca2+ entry. These findings suggest that differences in mitochondrial function, rather than MCU complex composition, contribute to the sex-specific differences in vasodilation.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cell Biology
Melanie Paillard, Kai-Ting Huang, David Weaver, Jonathan P. Lambert, John W. Elrod, Gyorgy Hajnoczky
Summary: Heart failure is a significant cause of hospitalization and mortality worldwide. The regulation of mitochondrial calcium handling appears to play a crucial role in the progression of heart failure. This study investigated the protein composition of mitochondrial calcium uniporter (mtCU) in human hearts and observed alterations in the regulators MICU1 and MICU2, suggesting a possible role for adaptive/maladaptive changes in mtCU composition in the initiation/progression of human heart failure.
Article
Multidisciplinary Sciences
Akshar Patel, Matthew Simkulet, Soumya Maity, Manigandan Venkatesan, Anastasios Matzavinos, Muniswamy Madesh, B. Rita Alevriadou
Summary: This study found that under shear stress, the increase in mitochondrial Ca2+ levels is associated with the function and energy metabolism of endothelial cells. Activation of the mechanosensitive channel Piezo1 and the endothelial nitric oxide synthase eNOS are involved in this process.
SCIENTIFIC REPORTS
(2022)
Review
Physiology
Omar Lozano, Patricio Marcos, Felipe de Jesus Salazar-Ramirez, Anay F. Lazaro-Alfaro, Luis Sobrevia, Gerardo Garcia-Rivas
Summary: Cardiovascular diseases (CVDs) are the leading cause of death worldwide and are associated with mitochondrial dysfunction, specifically in the regulation of Ca2+ uptake. Modulating the activity of the mitochondrial Ca2+ uniporter (MCU) complex has been suggested as a potential therapeutic approach for CVDs.
Article
Cell Biology
Agnese De Mario, Anna Tosatto, Julia Marie Hill, Janos Kriston-Vizi, Robin Ketteler, Denis Vecellio Reane, Gino Cortopassi, Gyorgy Szabadkai, Rosario Rizzuto, Cristina Mammucari
Summary: In this study, amorolfine and benzethonium were identified as positive and negative modulators of the mitochondrial calcium uniporter (MCU), demonstrating their potential roles in muscle development and cancer progression.
Editorial Material
Cell Biology
Janane F. Rahbani, Lawrence Kazak
Summary: The study suggests that the mitochondrial calcium uniporter binds uncoupling protein 1 via the MCU regulator to form a protein complex called the thermoporter. Through this complex, calcium influx into the mitochondrial matrix is enhanced, leading to increased NADH production and thermogenesis in brown adipose tissue.
Review
Cell Biology
Don-On Daniel Mak, J. Kevin Foskett
Letter
Cell Biology
Horia Vais, Jessica E. Tanis, Marioly Mueller, Riley Payne, Karthik Mallilankaraman, J. Kevin Foskett
Article
Behavioral Sciences
Michael G. Tordoff, Tiffany R. Aleman, Hillary T. Ellis, Makoto Ohmoto, Ichiro Matsumoto, Val I. Shestopalov, Claire H. Mitchell, J. Kevin Foskett, Rachel L. Poole
Correction
Biochemistry & Molecular Biology
Patrick J. Doonan, Harish C. Chandramoorthy, Nicholas E. Hoffman, Xueqian Zhang, Cesar Cardenas, Santhanam Shanmughapriya, Sudarsan Rajan, Sandhya Vallem, Xiongwen Chen, J. Kevin Foskett, Joseph Y. Cheung, Steven R. Houser, Muniswamy Madesh
Review
Cardiac & Cardiovascular Systems
J. Kevin Foskett, Benjamin Philipson
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2015)
Correction
Cell Biology
Karthik Mallilankaraman, Cesar Cardenas, Patrick J. Doonan, Harish C. Chandramoorthy, Krishna M. Irrinki, Tuende Golenar, Gyoergy Csordas, Priyanka Madireddi, Jun Yang, Marioly Mueller, Russell Miller, Jill E. Kolesar, Jordi Molgo, Brett Kaufman, Gyoergy Hajnoczky, J. Kevin Foskett, Muniswamy Madesh
NATURE CELL BIOLOGY
(2015)
Review
Physiology
Zhongming Ma, Jessica E. Tanis, Akiyuki Taruno, J. Kevin Foskett
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2016)
Article
Biochemical Research Methods
Don-On Daniel Mak, King-Ho Cheung, Patrick Toglia, J. Kevin Foskett, Ghanim Ullah
PLOS COMPUTATIONAL BIOLOGY
(2015)
Article
Cell Biology
Horia Vais, Karthik Mallilankaraman, Don-On Daniel Mak, Henry Hoff, Riley Payne, Jessica E. Tanis, J. Kevin Foskett
Article
Biochemistry & Molecular Biology
Russell A. Miller, Yuji Shi, Wenyun Lu, David A. Pirman, Aditi Jatkar, Matthew Blatnik, Hong Wu, Cesar Cardenas, Min Wan, J. Kevin Foskett, Junyoung O. Park, Yiyi Zhang, William L. Holland, Joshua D. Rabinowitz, Morris J. Birnbaum
Correction
Biochemistry & Molecular Biology
Russell A. Miller, Yuji Shi, Wenyun Lu, David A. Pirman, Aditi Jatkar, Matthew Blatnik, Hong Wu, Cesar Cardenas, Min Wan, J. Kevin Foskett, Junyoung O. Park, Yiyi Zhang, William L. Holland, Joshua D. Rabinowitz, Morris J. Birnbaum
Review
Physiology
Akiyuki Taruno, Kengo Nomura, Tsukasa Kusakizako, Zhongming Ma, Osamu Nureki, J. Kevin Foskett
Summary: Recent research has made significant progress in understanding the molecular mechanisms behind taste transduction, including the identification of specific taste sensors and components for each taste sensation. The discovery of unconventional chemical synapses and insights into the structures of ion channels have provided new perspectives in the field of taste sensation and neurotransmission.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Horia Vais, Riley Payne, Usha Paudel, Carmen Li, J. Kevin Foskett
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Cristina Aguirre-Portoles, Riley Payne, Aspen Trautz, J. Kevin Foskett, Christopher A. Natale, John T. Seykora, Todd W. Ridky
Summary: Testosterone signaling through ZIP9 mediates some of the sex differences in melanoma, and drugs that target AR can be repurposed to block ZIP9 and inhibit melanoma in males.
Article
Cell Biology
Zhongming Ma, Usha Paudel, J. Kevin Foskett
Summary: The intensity of taste is strongly influenced by temperature, but there is a lack of comprehensive understanding about the physiological, hedonic, and commercial implications of this relationship. This study used patch-clamp electrophysiology to investigate the effects of temperature on taste-bud cell electrical excitability, revealing that temperature significantly affects the electrical activity of type II taste-bud cells. These findings provide a mechanism for understanding how temperature influences taste sensitivity and perception.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
