Article
Immunology
Elena Putscher, Michael Hecker, Brit Fitzner, Nina Boxberger, Margit Schwartz, Dirk Koczan, Peter Lorenz, Uwe Klaus Zettl
Summary: This study found that genetic variants from MS risk loci affect pre-mRNA splicing. These findings substantiate the role of alternative splicing events in the genetics of MS. Further research on how disease-causing genetic variants modify the interactions between splicing regulatory sequence elements and RNA-binding proteins can help deepen our understanding of the genetic susceptibility to MS.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
James D. D. Fackenthal
Summary: Cancer is a leading cause of death worldwide. Alternative mRNA splicing has recently been recognized as a major contributor to cancer initiation and progression. Cancer cells exhibit different distribution of splicing variants compared to non-cancer cells, and they are more sensitive to drugs targeting the splicing regulatory network. Certain recurring splicing variants have been shown to contribute to tumor progression, while some disruption events result from mutations in splicing regulatory sequences or shifts in balance among naturally occurring splicing variants of mRNAs involved in cell cycle progression and apoptosis regulation. This review explores cancer-related alternative splicing events and potential therapies.
Article
Endocrinology & Metabolism
Weiwei Liang, Fangfang Sun
Summary: This study identified 1005 survival-associated alternative splicing events in ACC patients and constructed a prediction model for prognostic genes, providing biomarkers for disease progression and potentially rich source of novel therapeutic targets. A nomogram integrating clinical-pathological factors and riskscore was developed for predicting 1- and 3-year survival rate.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Oncology
Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee K. Kim
Summary: Alternative pre-mRNA splicing plays a critical role in generating multiple mRNA and increasing protein diversity. Specific splicing isoforms have been found to be important in cellular processes, particularly in regulating cell numbers. Abnormal expression of splicing isoforms and factors, as well as disruptions in splicing caused by genetic mutations, are implicated in cancer development and progression. Alternative splicing has potential as a therapeutic target for cancer.
CANCER CELL INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Yang Pan, John W. Phillips, Beatrice D. Zhang, Miyako Noguchi, Eric Kutschera, Jami McLaughlin, Pavlo A. Nesterenko, Zhiyuan Mao, Nathanael J. Bangayan, Robert Wang, Wendy Tran, Harry T. Yang, Yuanyuan Wang, Yang Xu, Matthew B. Obusan, Donghui Cheng, Alex H. Lee, Kathryn E. Kadash-Edmondson, Ameya Champhekar, Cristina Puig-Saus, Antoni Ribas, Robert M. Prins, Christopher S. Seet, Gay M. Crooks, Owen N. Witte, Yi Xing
Summary: This study describes a computational platform called IRIS that can discover tumor antigens derived from alternative splicing, providing potential targets for TCR and CAR-T immunotherapies. Through the analysis of transcriptomics and immuno-peptidomics data, the study demonstrates that the predicted targets by IRIS can bind with HLA molecules. The study illustrates the contribution of alternative splicing to the repertoire of tumor antigens and demonstrates the utility of IRIS in discovering AS-derived antigens and expanding cancer immunotherapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Praveen Kumar Kathare, Ruijiao Xin, Abirama Sundari Ganesan, Viviana M. June, Anireddy S. N. Reddy, Enamul Huq
Summary: Light signals have significant effects on the physiology, growth, and development of plants. SWAP1, an RNA-binding splicing factor, regulates light-regulated alternative splicing by interacting with other splicing factors and photoactivated phyB.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Joseph T. Smith Jr, Brianna Tylec, Arunasalam Naguleswaran, Isabel Roditi, Laurie K. Read
Summary: This study reveals the importance of mitochondrial mRNA editing in the developmental regulation of Trypanosoma brucei. The researchers found that temperature reduction and depletion of the differentiation-repressive kinase RDK1 can affect the metabolism of Trypanosoma brucei by altering the editing of mitochondrial cytochrome mRNAs.
Article
Pharmacology & Pharmacy
Ni Li, Yan Li, Xiaowan Han, Jing Zhang, Jiangxue Han, Xinhai Jiang, Weizhi Wang, Yang Xu, Yanni Xu, Yu Fu, Shuyi Si
Summary: The LXR agonist T0901317 inhibits lipopolysaccharide-induced inflammation by regulating the alternative splicing of MyD88 mRNA involved in the TLR4 signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Honey Bokharaie, Walter Kolch, Aleksandar Krstic
Summary: Alternative splicing plays an important role in cancer, and one common mechanism of vemurafenib resistance in malignant melanoma involves alternative splicing of the BRAF gene. Our study shows that alternative splicing in vemurafenib-resistant cells can affect cell motility and melanin synthesis. The results highlight the need for further investigation of aberrant alternative splicing in patients with melanoma.
Article
Cell Biology
Yanfei Wang, Cuiqiao Zhang, Wu Peng, Haibo Du, Yuehui Xi, Zhigang Xu
Summary: This study further explored the role of RBM24 in hearing and balance through conditional knockout mice and found that the loss of RBM24 results in severe deficits. It was observed that the development of hair cells was significantly affected, leading to degeneration and disorganization of the hair bundles. RNAseq analysis also identified several genes whose mRNA stability or pre-mRNA alternative splicing is affected by RBM24 knockout.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Immunology
Felix Ostwaldt, Bruna Los, Florian Heyd
Summary: Intracellular trafficking and protein secretion are regulated by multiple layers of control, especially during lymphocyte activation and differentiation. Alternative splicing plays a significant role in dynamically adapting intracellular traffic and protein secretion in different B cell subtypes, while NERF/ELF2 regulates the expression of COPII-related genes in a cell type-specific manner. T cells and B cells utilize different adaptive strategies in adjusting their secretory machineries during effector and memory cell generation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Songbiao Chen, Bichen Miao, Nannan Chen, Caiyi Chen, Ting Shao, Xuezhi Zhang, Lingling Chang, Xiujuan Zhang, Qian Du, Yong Huang, Dewen Tong
Summary: The study identified SYNCRIP as a critical molecule in the alternative splicing process of PPV mRNA, which is significantly up-regulated by PPV infection and promotes the cleavage of NS1 mRNA into NS2 mRNA. This protein reveals a novel function and provides a potential target for antiviral intervention in controlling porcine parvovirus disease.
VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mingtian Deng, Baobao Chen, Zifei Liu, Yongjie Wan, Dongxu Li, Yingnan Yang, Feng Wang
Summary: YBX1 plays important roles in maternal mRNA decay, alternative splicing, and transcriptional activity during early embryogenesis. These findings broaden our understanding of YBX1 functions.
CELL AND BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xiaohong Yao, Chaofan Wang, Weiran Yu, Longjie Sun, Zheng Lv, Xiaomei Xie, Shuang Tian, Lu Yan, Hua Zhang, Jiali Liu
Summary: This study found that SRSF1 plays an important role in mouse granulosa cells. Specific knockout of SRSF1 leads to inhibition of follicular development, decreased granulosa cell proliferation, and increased apoptosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Genetics & Heredity
Zhongjing Su, Dongyang Huang
Summary: Alternative splicing plays a critical role in the regulation of immune cell development and immune activity, with aberrant splicing leading to abnormal immune responses. This review summarizes recent discoveries on immune-associated alternative splice variants, clinical disorders, and regulatory mechanisms.
Article
Biochemistry & Molecular Biology
Thomas A. Hilimire, Ryan P. Bennett, Ryan A. Stewart, Pablo Garcia-Miranda, Alex Blume, Jordan Becker, Nathan Sherer, Eric D. Helms, Samuel E. Butcher, Harold C. Smith, Benjamin L. Miller
ACS CHEMICAL BIOLOGY
(2016)
Article
Pharmacology & Pharmacy
Ryan P. Bennett, Ryan A. Stewart, Priscilla A. Hogan, Roger G. Ptak, Marie K. Mankowski, Tracy L. Hartman, Robert W. Buckheit, Beth A. Snyder, Jason D. Salter, Guillermo A. Morales, Harold C. Smith
ANTIVIRAL RESEARCH
(2016)
Review
Biochemistry & Molecular Biology
Harold C. Smith
Article
Biochemistry & Molecular Biology
Bogdan Polevoda, Rebecca Joseph, Alan E. Friedman, Ryan P. Bennett, Rebecca Greiner, Thareendra De Zoysa, Ryan A. Stewart, Harold C. Smith
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Multidisciplinary Sciences
Chad A. Galloway, Sonal Dalvi, Sandy S. C. Hung, Leslie A. MacDonald, Lisa R. Latchney, Raymond C. B. Wong, Robyn H. Guymer, David A. Mackey, David S. Williams, Mina M. Chung, David M. Gamm, Alice Pebay, Alex W. Hewitt, Ruchira Singh
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Microbiology
Elysia A. Masters, Karen L. de Mesy Bentley, Ann Lindley Gill, Stephanie P. Hao, Chad A. Galloway, Alec T. Salminen, Diamond R. Guy, James L. McGrath, Hani A. Awad, Steven R. Gill, Edward M. Schwarz
Article
Orthopedics
Karen L. de Mesy Bentley, Chad A. Galloway, Gowrishankar Muthukrishnan, Scott R. Echternacht, Elysia A. Masters, Stephan Zeiter, Edward M. Schwarz, Jonathan I. Leckenby
Summary: Recent breakthroughs in understanding orthopaedic infections have been made through advances in transmission electron microscopy imaging of murine models, particularly in revealing the colonization process of Staphylococcus aureus in the bone networks. Three-dimensional reconstruction highlighted adjacent uninfected canaliculi, suggesting viable osteocytes may respond and resist infection in chronic osteomyelitis, opening new research directions.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Orthopedics
Elysia A. Masters, Stephanie P. Hao, H. Mark Kenney, Yugo Morita, Chad A. Galloway, Karen L. de Mesy Bentley, Benjamin F. Ricciardi, Brendan F. Boyce, Edward M. Schwarz, Irvin Oh
Summary: Osteomyelitis, a severe complication of orthopedic surgery, is commonly caused by Staphylococcus aureus and Group B Streptococcus. In a murine model, S. aureus induced more severe damage compared to S. agalactiae, with the former showing greater virulence and the latter exhibiting a vasculotropic mode of infection.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Virology
Ryan P. Bennett, Courtney L. Finch, Elena N. Postnikova, Ryan A. Stewart, Yingyun Cai, Shuiqing Yu, Janie Liang, Julie Dyall, Jason D. Salter, Harold C. Smith, Jens H. Kuhn
Summary: Filoviruses, such as Ebola virus and Marburg virus, pose significant threats to human health. Despite progress in vaccine and antibody-based therapies, there remains a need for efficacious small-molecule therapeutics. A novel high-throughput screening assay has shown promise in quickly identifying potent medical countermeasures against Ebola virus and its relatives.
Article
Biology
Cynthia Tang, Jimin Han, Sonal Dalvi, Kannan Manian, Lauren Winschel, Stefanie Volland, Celia A. Soto, Chad A. Galloway, Whitney Spencer, Michael Roll, Caroline Milliner, Vera L. Bonilha, Tyler B. Johnson, Lisa Latchney, Jill M. Weimer, Erika F. Augustine, Jonathan W. Mink, Vamsi K. Gullapalli, Mina Chung, David S. Williams, Ruchira Singh
Summary: Mutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, affecting the phagocytosis of photoreceptor outer segments by retinal pigment epithelium (RPE) cells. Patient-derived cells show that CLN3 is essential for RPE cell structure and phagocytosis, suggesting potential for gene therapy targeting RPE cells in treating CLN3 disease.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Humberto Mestre, Natasha Verma, Thom D. Greene, LiJing A. Lin, Antonio Ladron-de-Guevara, Amanda M. Sweeney, Guojun Liu, V. Kaye Thomas, Chad A. Galloway, Karen L. de Mesy Bentley, Maiken Nedergaard, Rupal I. Mehta
Summary: The study reveals that the pia mater, previously thought to confine fluid flow around blood vessels in the brain, is actually perforated and permissive to cerebrospinal fluid (CSF) flow. The pial structure and function are found to be associated with CSF transport and amyloid clearance, suggesting a previously unrecognized role of pia in regulating aging and disease in the brain.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Trishna Shah, Sue E. Leurgans, Rashi I. Mehta, Jingyun Yang, Chad A. Galloway, Karen de Mesy L. Bentley, Julie A. Schneider, Rupal I. Mehta
Summary: The study investigates the microstructure and functions of arachnoid granulations (AG) in the brain. It shows that AG harbor immune cells and communicate with perisinus spaces, suggesting their roles in neuroimmune functions and as transarachnoidal passageways. These findings have implications for glymphatic-lymphatic coupling and mechanisms of diseases.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Cell Biology
Sonal Dalvi, Chad A. Galloway, Lauren Winschel, Ali Hashim, Celia Soto, Cynthia Tang, Leslie A. MacDonald, Ruchira Singh
CELL DEATH DISCOVERY
(2019)
Article
Ophthalmology
Chad A. Galloway, Sonal Dalvi, Audra M. A. Shadforth, Shuko Suzuki, Molly Wilson, David Kuai, Ali Hashim, Leslie A. MacDonald, David M. Gamm, Damien G. Harkin, Ruchira Singh
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Thomas A. Hilimire, Jeffrey M. Chamberlain, Viktoriya Anokhina, Ryan P. Bennett, Oliver Swart, Jason R. Myers, John M. Ashton, Ryan A. Stewart, Aaron L. Featherston, Kathleen Gates, Eric D. Helms, Harold C. Smith, Stephen Dewhurst, Benjamin L. Miller
ACS CHEMICAL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
