An efficient blue-white screening system for markerless deletions and stable integrations in Streptomyces chromosomes based on the blue pigment indigoidine biosynthetic gene bpsA

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Quantitation of secreted proteins using mCherry fusion constructs and a fluorescent microplate reader

(2015) Tyler Duellman et al.   ANALYTICAL BIOCHEMISTRY

An efficient blue-white screening based gene inactivation system for Streptomyces

(2015) Pengwei Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Complete genome sequence of Streptomyces lividans TK24

(2015) Christian Rückert et al.   JOURNAL OF BIOTECHNOLOGY

Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes

(2015) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces

(2015) Renata Knirschova et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Iterative marker excision system

(2014) Maksym Myronovskyi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes

(2014) Lorena T. Fernández-Martínez et al.   Scientific Reports

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Activation and identification of five clusters for secondary metabolites inStreptomyces albus J1074

(2014) Carlos Olano et al.   Microbial Biotechnology

Genome engineering in actinomycetes using site-specific recombinases

(2013) Maksym Myronovskyi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Actinomycetes genome engineering approaches

(2012) Theresa Siegl et al.   ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY

An indigoidine biosynthetic gene cluster from Streptomyces chromofuscus ATCC 49982 contains an unusual IndB homologue

(2012) Dayu Yu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Recombinant protein production and streptomycetes

(2011) Jozef Anné et al.   JOURNAL OF BIOTECHNOLOGY

Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms)

(2011) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

I-SceI endonuclease: a new tool for DNA repair studies and genetic manipulations in streptomycetes

(2010) Theresa Siegl et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Identification and characterization of an indigoidine-like gene for a blue pigment biosynthesis in Streptomyces aureofaciens CCM 3239

(2010) R. Novakova et al.   FOLIA MICROBIOLOGICA

Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria

(2008) M.-P. Dubeau et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Marker removal from actinomycetes genome using Flp recombinase

(2008) Marta Fedoryshyn et al.   GENE