Iterative marker excision system

Genome engineering in actinomycetes using site-specific recombinases

(2013) Maksym Myronovskyi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Design, construction and characterisation of a synthetic promoter library for fine-tuned gene expression in actinomycetes

(2013) Theresa Siegl et al.   METABOLIC ENGINEERING

Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor

(2013) M. M. Rudolph et al.   MICROBIOLOGY-SGM

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

antiSMASH 2.0—a versatile platform for genome mining of secondary metabolite producers

(2013) Kai Blin et al.   NUCLEIC ACIDS RESEARCH

An Efficient Procedure for Marker-Free Mutagenesis of S. coelicolor by Site-Specific Recombination for Secondary Metabolite Overproduction

(2013) Bo Zhang et al.   PLoS One

Engineered Streptomyces avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

(2013) Mamoru Komatsu et al.   ACS Synthetic Biology

Site-Specific Recombination Strategies for Engineering Actinomycete Genomes

(2012) Simone Herrmann et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters

(2012) Kozo Ochi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Magic Bullet for Antibiotic Discovery

(2012) Kozo Ochi et al.   CHEMISTRY & BIOLOGY

Chemical Perturbation of Secondary Metabolism Demonstrates Important Links to Primary Metabolism

(2012) Arryn Craney et al.   CHEMISTRY & BIOLOGY

Synthetic Biology of secondary metabolite biosynthesis in actinomycetes: Engineering precursor supply as a way to optimize antibiotic production

(2012) Wolfgang Wohlleben et al.   FEBS LETTERS

β-Glucuronidase as a Sensitive and Versatile Reporter in Actinomycetes

(2011) Maksym Myronovskyi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced Flavonoid Production in Streptomyces venezuelae via Metabolic Engineering

(2011) Sung Ryeol Park   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

A phage protein that binds φC31 integrase to switch its directionality

(2011) Thanafez Khaleel et al.   MOLECULAR MICROBIOLOGY

antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences

(2011) Marnix H. Medema et al.   NUCLEIC ACIDS RESEARCH

A system for the targeted amplification of bacterial gene clusters multiplies antibiotic yield in Streptomyces coelicolor

(2011) T. Murakami et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters

(2010) Richard H. Baltz   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Genetic factors that influence moenomycin production in streptomycetes

(2010) Roman Makitrynskyy et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites

(2010) Hong Gao et al.   Protein & Cell

Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters

(2010) Juan Pablo Gomez-Escribano et al.   Microbial Biotechnology

Cloning and Heterologous Expression of the Cyclooctatin Biosynthetic Gene Cluster Afford a Diterpene Cyclase and Two P450 Hydroxylases

(2009) Seung-Young Kim et al.   CHEMISTRY & BIOLOGY

Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production

(2009) Jun Lee et al.   Microbial Cell Factories

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

(2009) Hiroko Tsuruta et al.   PLoS One

Functional expression of the Cre recombinase in actinomycetes

(2008) Marta Fedoryshyn et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineered production of iso-migrastatin in heterologous Streptomyces hosts

(2008) Zhiyang Feng et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Marker removal from actinomycetes genome using Flp recombinase

(2008) Marta Fedoryshyn et al.   GENE

Genome Sequence of the Streptomycin-Producing Microorganism Streptomyces griseus IFO 13350

(2008) Y. Ohnishi et al.   JOURNAL OF BACTERIOLOGY

Antibiotic Overproduction inStreptomyces coelicolorA3(2) Mediated by Phosphofructokinase Deletion

(2008) Irina Borodina et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette

(2008) E. Sangiorgi et al.   NUCLEIC ACIDS RESEARCH