Article
Agricultural Engineering
Xuyang Zhu, Kang Zhang, Hui Luo, Jing Wu
Summary: The bottleneck of recombinant protein production in microbial cell factories is sometimes caused by limited manipulable targets and a lack of gene annotation related to protein expression. In this study, the novel functions of PonA in Bacillus subtilis recombinant protein expression were described, and the mechanism of its chaperone activity was analyzed. Overexpression of PonA led to a significant increase in the expression of hyperthermophilic amylase in shake flasks and fed-batch processes. Increased cell diameter and reinforced cell walls were observed in PonA-overexpressing strains. The FN3 structural domain and the natural dimeric structure of PonA may play critical roles in its chaperone activity. These findings suggest that PonA can be an effective target for modifying the expression of recombinant proteins in B. subtilis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Silja Vaelimets, Patricia Pedetti, Ludovika Jessica Virginia, Mai Ngoc Hoang, Michael Sauer, Clemens Peterbauer
Summary: Small laccases from Streptomyces coelicolor, Streptomyces viridosporus, and Amycolatopsis 75iv2 were successfully expressed in both Gram-positive expression hosts B. subtilis and S. lividans. However, the extracellular activities of the laccases were significantly higher in S. lividans compared to B. subtilis.
MICROBIAL CELL FACTORIES
(2023)
Article
Biology
Xian Zhang, Ruiqi Lu, Qiang Wang, Mengkai Hu, Zhiyue Li, Meijuan Xu, Taowei Yang, Rongzhen Zhang, Zhiming Rao
Summary: This study successfully achieved the production of D-tagatose in a one-step process using a D-alanine-deficient heterologous gene expression system through integrated expression and co-expression plasmids method. The innovative integration of the beta-galactosidase gene in recombinant is promising for high-value product production.
Article
Biochemistry & Molecular Biology
Seunghyun Sim, Yue Hui, David A. Tirrell
Summary: The incorporation of living cells into materials presents both challenges and possibilities. In this study, a new class of 3D-printable materials based on poly(N-hydroxymethylacrylamide) is developed, which allows the cross-linking of spore-forming bacteria into a polymeric scaffold. These embedded cells and spores can become metabolically active and produce proteins after re-swelling in nutrient-rich media. The leak-free scaffold enables protein production while preventing cell escape. This research demonstrates the potential utility of living composite materials in various applications.
Review
Biotechnology & Applied Microbiology
Haiquan Yang, Jinfeng Qu, Wei Zou, Wei Shen, Xianzhong Chen
Summary: This article comprehensively reviews recent studies on recombinant protein production in Bacillus subtilis, focusing on strain optimization, expression enhancement, secretion improvement, surface display of proteins, and fermentation optimization. Novel methods and future challenges for recombinant protein production in B. subtilis are proposed.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yaqing Zhu, Linlin Wang, Kaixuan Zheng, Ping Liu, Wenkang Li, Jian Lin, Wenjing Liu, Shoushui Shan, Liqin Sun, Hailing Zhang
Summary: Collagenase was successfully recombinantly expressed in Bacillus subtilis and its properties were characterized. The activity of the recombinant collagenase was significantly improved by optimizing the culture conditions.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Aisling Brady, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Sara Zamora-Caballero, Alonso Felipe-Ruiz, Jorge Val-Calvo, Wilfried J. J. Meijer, Alberto Marina, Jose R. Penades
Summary: Some Bacillus-infecting bacteriophages utilize a peptide-based communication system, called arbitrium, to coordinate the lysis-lysogeny decision. AimR, a key player in this system, is involved in a complex network of regulation. The SP beta phage, possessing a functional arbitrium system, can optimize production of infective particles while preserving the number of surviving cells, which increases phage persistence in nature.
Article
Agriculture, Dairy & Animal Science
Yunpeng Xu, Qiong Wu, Li Bai, Guangqing Mu, Yanfeng Tuo, Shujuan Jiang, Xuemei Zhu, Fang Qian
Summary: The study cloned a novel thermostable neutral lactase from Bacillus coagulans T242, which showed optimum hydrolytic activity under certain conditions, indicating its potential application in the dairy industry.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Penghao Lv, Xuesong Zhang, Mengze Song, Guijuan Hao, Fangkun Wang, Shuhong Sun
Summary: In this study, we constructed a recombinant Bacillus subtilis that can secrete a multi-epitope protein. Oral immunization of chickens with this recombinant B. subtilis induced high levels of specific IgG and specific secretory immunoglobulin A (sIgA), as well as increased levels of IL-2 and T cell-mediated immunity. This recombinant B. subtilis effectively protected chickens against S. Enteritidis and reduced pathological damage to the spleen and jejunum, indicating its potential as a mucosal vaccine candidate for animals to defend against S. Enteritidis in the future.
VETERINARY MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jian-Hong Tang, Hao Li, Chaonan Yuan, Giacomo Parigi, Claudio Luchinat, Thomas J. Meade
Summary: We have developed a new enzyme-activatable agent for real-time detection of bio-events in whole animals using magnetic resonance imaging. This new agent has a low magnetic resonance relaxivity in the off-state but generates significantly higher relaxivity upon enzymatic processing. Mechanistic insights regarding the magnetic resonance signal enhancement at the molecular scale were obtained through single X-ray crystal and nuclear magnetic relaxation dispersion analyses.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biotechnology & Applied Microbiology
Patrick Lenz, Fabienne Hilgers, Alina Burmeister, Leonie Zimmermann, Kristina Volkenborn, Alexander Grunberger, Dietrich Kohlheyer, Thomas Drepper, Karl-Erich Jaeger, Andreas Knapp
Summary: The iSplit GFP assay has been successfully developed for the detection, quantification, and online monitoring of recombinant protein production in Bacillus subtilis during cultivation. This system allows for monitoring protein production at a single-cell level, providing insights into intracellular protein distribution and culture heterogeneity.
MICROBIAL CELL FACTORIES
(2021)
Article
Engineering, Environmental
Vasanth Kumar Vaithyanathan, Hubert Cabana, Vinoth Kumar Vaidyanathan
Summary: The study evaluated the effect of four different pre-treatment strategies on biosolids valorization and TrOCs removal during bioaugmentation with Bacillus subtilis. Results showed that using B. subtilis aided aerobic digestion is an effective process for biosolids treatment, and different pre-treatment strategies impact the removal of TrOCs and TSS.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Food Science & Technology
Meng Zhao, Duanmu Liu, Zhihong Liang, Kunlun Huang, Xiaoyan Wu
Summary: The proteins produced by Bacillus subtilis CW14 and β-glucanase can effectively inhibit Aspergillus ochraceus, increase OTA production, and may affect the secondary metabolism of Aspergillus ochraceus by activating the CWI signaling pathway.
Article
Mathematics, Applied
Jianyu Hu, Xiaoli Chen, Jinqiao Duan
Summary: We investigate the quantitative network of gene expression dynamics in the competence development of Bacillus subtilis, quantifying the most probable transition pathways for both excitable and bistable dynamics and analyzing how noise intensity affects the transition phenomena.
Article
Biotechnology & Applied Microbiology
Zhuang Yao, Yu Meng, Huong Giang Le, Se Jin Lee, Hye Sung Jeon, Ji Yeon Yoo, Jeong Hwan Kim
Summary: In this study, the aprE3-5 promoter in Bacillus subtilis CH3-5 was replaced with stronger promoters, leading to increased fibrinolytic activity. The use of the P10 promoter resulted in the highest fibrinolytic activity, and changing the initiation codon from GTG to ATG further enhanced this activity. These results suggest that significant overproduction of fibrinolytic enzymes can be achieved through appropriate promoter modification.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Ecology
Frank Unfried, Stefan Becker, Craig S. Robb, Jan-Hendrik Hehemann, Stephanie Markert, Stefan E. Heiden, Tjorven Hinzke, Doerte Becher, Greta Reintjes, Karen Krueger, Burak Avci, Lennart Kappelmann, Richard L. Hahnke, Tanja Fischer, Jens Harder, Hanno Teeling, Bernhard Fuchs, Tristan Barbeyron, Rudolf I. Amann, Thomas Schweder
Article
Ecology
Lennart Kappelmann, Karen Krueger, Jan-Hendrik Hehemann, Jens Harder, Stephanie Markert, Frank Unfried, Doerte Becher, Nicole Shapiro, Thomas Schweder, Rudolf I. Amann, Hanno Teeling
Article
Ecology
Hanna Koch, Alexandra Duerwald, Thomas Schweder, Beatriz Noriega-Ortega, Silvia Vidal-Melgosa, Jan-Hendrik Hehemann, Thorsten Dittmar, Heike M. Freese, Doerte Becher, Meinhard Simon, Matthias Wietz
Article
Microbiology
Florian Goetz, Petra Pjevac, Stephanie Markert, Jesse McNichol, Doerte Becher, Thomas Schweder, Marc Mussmann, Stefan M. Sievert
ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Dimitri Meier, Petra Pjevac, Wolfgang Bach, Stephanie Markert, Thomas Schweder, John Jamieson, Sven Petersen, Rudolf Amann, Anke Meyerdierks
ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Ecology
Karen Krueger, Meghan Chafee, T. Ben Francis, Tijana Glavina del Rio, Doerte Becher, Thomas Schweder, Rudolf I. Amann, Hanno Teeling
Article
Biotechnology & Applied Microbiology
Marie-Katherin Zuehlke, Rabea Schlueter, Annett Mikolasch, Ann-Kristin Henning, Martin Giersberg, Michael Lalk, Gotthard Kunze, Thomas Schweder, Tim Urich, Frieder Schauer
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Microbiology
Tjorven Hinzke, Manuel Kleiner, Corinna Breusing, Horst Felbeck, Robert Hasler, Stefan M. Sievert, Rabea Schluter, Philip Rosenstiel, Thorsten B. H. Reusch, Thomas Schweder, Stephanie Markert
Article
Microbiology
Alexandra Duerwald, Marie-Katherin Zuehlke, Rabea Schlueter, Rebecca Gebbe, Daniel Bartosik, Frank Unfried, Doerte Becher, Thomas Schweder
Summary: The study revealed that Pseudoalteromonas distincta ANT/505 produces outer membrane vesicles and vesicle chains on different carbon sources, equipped with highly substrate-specific proteins to facilitate rapid sensing and response in nutritionally deprived environments. This adaptation may also help in efficient carbohydrate processing and reduce loss of substrates and enzymes in the aquatic ecosystem.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marcus Baeumgen, Theresa Dutschei, Daniel Bartosik, Christoph Suster, Lukas Reisky, Nadine Gerlach, Christian Stanetty, Marko D. Mihovilovic, Thomas Schweder, Jan-Hendrik Hehemann, Uwe T. Bornscheuer
Summary: This study identified a new enzymatic degradation pathway of marine bacterium Formosa agariphila for ulvan oligosaccharides, which involves a new dehydratase P29_PDnc acting on the nonreducing end, forming an unsaturated uronic acid residue as substrate for GH105 glycoside hydrolases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Stefan Brott, Francois Thomas, Maike Behrens, Karen Methling, Daniel Bartosik, Theresa Dutschei, Michael Lalk, Gurvan Michel, Thomas Schweder, Uwe T. Bornscheuer
Summary: Marine bacteria Zobellia galactanivorans utilize the ribulose monophosphate pathway to detoxify formaldehyde, and the genes encoding key enzymes in this pathway are crucial for maintaining formaldehyde resistance.
Article
Ecology
Irena Beidler, Craig S. Robb, Silvia Vidal-Melgosa, Marie-Katherin Zuhlke, Daniel Bartosik, Vipul Solanki, Stephanie Markert, Dorte Becher, Thomas Schweder, Jan-Hendrik Hehemann
Summary: The presence of beta-mannan in diatom blooms suggests that marine microorganisms have a conserved enzymatic cascade for degrading beta-mannans from marine and terrestrial sources, highlighting the importance of this metabolic pathway in marine carbon cycling.
Article
Biotechnology & Applied Microbiology
Stefan Brott, Ki Hyun Nam, Francois Thomas, Theresa Dutschei, Lukas Reisky, Maike Behrens, Hanna C. C. Grimm, Gurvan Michel, Thomas Schweder, Uwe T. T. Bornscheuer
Summary: Marine algae produce complex polysaccharides that can be degraded by marine heterotrophic bacteria using carbohydrate-active enzymes. The degradation of the red algal polysaccharide porphyran involves oxidative demethylation, catalyzed by cytochrome P450 monooxygenase and its redox partners. Genes encoding zinc-dependent alcohol dehydrogenases (ADHs) were found near the genes for key enzymes in this process. The physiological role of these marine ADHs was investigated and it was found that they are required for the utilization of a specific substrate, G6Me.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Ecology
Florian U. U. Moeller, Craig W. W. Herbold, Arno Schintlmeister, Maria Mooshammer, Cherie Motti, Bettina Glasl, Katharina Kitzinger, Faris Behnam, Margarete Watzka, Thomas Schweder, Mads Albertsen, Andreas Richter, Nicole S. S. Webster, Michael Wagner
Summary: Marine sponges play a critical role in marine ecosystems as filter-feeders and reef-builders, and they harbor dense communities of microbes. These microbes contribute to the processing of dissolved organic matter (DOM). A study using metaproteogenomics and laboratory incubations found that a dominant gammaproteobacterial symbiont in a marine sponge expressed a pathway for the import and dissimilation of taurine, a common metabolite in marine sponges. The symbiont also imported and used DMSP as a carbon and sulfur source. These findings highlight the importance of biogenic sulfur compounds in the interaction between marine sponges and their microbial symbionts.
Article
Microbiology
Joachim Vater, Le Thi Thanh Tam, Jennifer Jaehne, Stefanie Herfort, Christian Blumenscheit, Andy Schneider, Pham Thi Luong, Le Thi Phuong Thao, Jochen Blom, Silke R. Klee, Thomas Schweder, Peter Lasch, Rainer Borriss
Summary: Seventeen bacterial strains capable of suppressing plant pathogens have been isolated from healthy Vietnamese crops and identified as members of the Bacillus cereus group. Through comprehensive analysis, including whole-genome sequencing and gene mining, their potential as biocontrol agents has been proven. The study found that these plant-associated strains contain various antimicrobial peptides, most of which have been shown to enhance plant growth and suppress plant pathogens. However, the presence of virulence genes may restrict their application in agriculture.
