Review
Biochemical Research Methods
Gregory Bauwens, Aleksandra Gorska, Giorgia Purcaro
Summary: Mineral oil hydrocarbons (MOH) are analyzed using the online coupled liquid chromatography-gas chromatography with flame ionization detection (LC-GC-FID) method, which lacks sufficient separation performance and may result in coelution of interfering compounds. Comprehensive two-dimensional gas chromatography (GC x GC) has proven to be relevant in overcoming the weaknesses of the LC-GC method and meeting the analytical requirements. This paper highlights the contributions of GC x GC in understanding MOH, meeting the determination requirements, and overcoming analytical and toxicological challenges.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Gregory Bauwens, Chiara Conchione, Nicola Sdrigotti, Sabrina Moret, Giorgia Purcaro
Summary: This study analyzed seven commercial feeds and found consistent results between two laboratories. The combination of liquid-gas chromatography and liquid chromatography coupled with comprehensive multidimensional gas chromatography, along with parallel detection using flame ionization detector and time-of-flight mass spectrometer, allowed for a more comprehensive characterization of the contaminants.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Raquel B. Gomez-Coca, Maria Del Carmen Perez-Camino, Wenceslao Moreda
Summary: The aim of this study was to determine the content of mineral oil hydrocarbons (MOH) in olive pomace oil and contribute to the monitoring required by EFSA. The study provided information on the concentrations and molecular mass profiles of MOH, as well as identified the structures that prove the harmless nature of the mineral oil aromatic hydrocarbon (MOAH) fraction. The results showed that the C25-C35 fraction is the main fraction to which humans are exposed through olive pomace oil, with concentrations dependent on the extraction process.
Article
Biochemical Research Methods
Gregory Bauwens, Sebastiano Panto, Giorgia Purcaro
Summary: The determination of mineral oil contamination levels in foods is a complex issue that requires methods like GC x GC for data interpretation and reliable integration of analytical results. The application of a new algorithm on an automated platform provides a novel solution for quantification and highlights the advantages of using an integrated platform.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Dieter Schrenk, Margherita Bignami, Laurent Bodin, Jesus del Mazo, Bettina Grasl-Kraupp, Christer Hogstrand, Laurentius (Ron) Hoogenboom, Jean-Charles Leblanc, Carlo Stefano Nebbia, Elsa Nielsen, Evangelia Ntzani, Annette Petersen, Salomon Sand, Tanja Schwerdtle, Christiane Vleminckx, Heather Wallace, Jan Alexander, Christophe Goldbeck, Konrad Grob, Jose angel Gomez Ruiz, Olaf Mosbach-Schulz, Marco Binaglia, James Kevin Chipman
Summary: Mineral oil hydrocarbons (MOH) consist of saturated hydrocarbons (MOSH) and aromatic hydrocarbons (MOAH). The complex composition of MOH makes it impossible to fully characterize chemically. Accumulation of MOSH differs in various tissues among different species. Liver damage and inflammation are observed in F344 rats due to specific accumulation of wax components, while no such effects are seen in SD rats or humans. Based on dietary exposure assessments, the present dietary exposure to MOSH is not a concern for human health. However, the presence of genotoxic and carcinogenic MOAH with three or more aromatic rings raises possible concerns for human health. The highest dietary exposure to MOAH is found in the young population. Overall, this article highlights the importance of understanding the effects and risks associated with the different components of mineral oil hydrocarbons. Rating: 8/10.
Article
Chemistry, Applied
Janira Jaen, Celia Domeno, Cristina Nerin
Summary: An analysis method was developed to detect chemical markers of mineral oil aromatic hydrocarbons (MOAH) from offset printing inks in food packaging materials. Different solid phase extraction procedures and gas chromatography coupled to mass spectrometry were used to identify the target analytes in both offset printing inks and food packages.
Article
Chemistry, Applied
Luca Menegoz Ursol, Chiara Conchione, Daniela Peroni, Andrea Carretta, Sabrina Moret
Summary: During the 2020-21 olive oil campaign, the contribution of harvesting operations to MOSH and MOAH contamination was studied. Hand-picked olives generally had low levels of MOSH and no quantifiable MOAH. However, contamination increased significantly after harvesting operations in 40% of the cases, with accidental leaks and contact with lubricated mechanical parts identified as important sources. Source identification was done through chromatographic traces obtained by HPLC-GC-FID, and confirmation and characterization were attempted using GC x GC with parallel FID/MS detection. Good harvesting practices are recommended to minimize contamination risks.
Article
Engineering, Manufacturing
Romy Fengler, Ludwig Gruber
Summary: This article compares migration studies of mineral oil hydrocarbons (MOH) from real papers with previous studies on surrogates and food simulants. The results show that the migration behavior of MOH can be depicted by the use of respective surrogates, which helps to simplify the analytical tasks. The study also verifies the applicability of food simulants in contact with MOH.
PACKAGING TECHNOLOGY AND SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Christopher Albert, Hans-Ulrich Humpf, Ludger Bruehl
Summary: A method for analyzing saturated and aromatic mineral oil hydrocarbons has been developed and evaluated in an interlaboratory trial. The method shows good reproducibility and precision for different oil samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Andrea Hochegger, Sabrina Moret, Lucie Geurts, Thomas Gude, Erich Leitner, Birgit Mertens, Susan O'Hagan, Fatima Pocas, Thomas J. Simat, Giorgia Purcaro
Summary: The key issue in the field of MOH risk analysis and management lies in the lack of standardized and validated analytical methods for good inter-laboratory reproducibility. There is also a major gap in exposure estimation due to limited surveys covering a wide range of foods and major sources of contamination other than packaging in paperboard. Additional toxicological studies are needed to better understand the hazards of MOH for a more comprehensive human health risk assessment.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Applied
Luca Menegoz Ursol, Chiara Conchione, Ana Srbinovska, Sabrina Moret
Summary: A rapid and solvent-saving method based on microwave-assisted saponification (MAS) followed by epoxidation and on-line LC-GC-FID has been optimized and validated for high-sensitivity determination of MOAH in extra virgin olive oils. The method was also applied for MOSH determination and showed that olive oils from the Italian market or oil mills were contaminated with variable amounts of MOSH and MOAH, while laboratory-extracted oils had low and rather constant levels of MOSH and no detectable MOAH.
Article
Food Science & Technology
Jose Luis Hidalgo Ruiz, Javier Arrebola Liebanas, Jose Luis Martinez Vidal, Antonia Garrido Frenich, Roberto Romero Gonzalez
Summary: A method has been developed for the determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) in edible oils, achieving similar quantification limits to online extraction methods. The method involves isolation of MOSH and MOAH in a silver nitrated silica gel stationary phase prior to analysis by gas chromatography-flame ionization detector (GC-FID). The method was validated and applied to a total of 27 samples of different types of oil, detecting MOSH up to 79.2 mg/kg and MOAH up to 22.4 mg/kg.
Article
Biochemical Research Methods
Alessia Arena, Mariosimone Zoccali, Ivana Bonaccorsi, Monica Mondello, Peter Q. Tranchida, Luigi Mondello
Summary: This study analyzed and discussed the contamination of mineral oil hydrocarbons in Citrus essential oils using an online liquid-gas chromatography system. Various degrees of mineral oil saturated hydrocarbon (MOSH) contamination were found in the cold-pressed (CP) essential oils, ranging from 10.7 to 338.4 mg kg(-1). However, no MOSH contamination was detected in the distilled essential oils. The level of mineral oil aromatic hydrocarbons in all samples was below the limit of quantification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Jing Jing Pan, Yan Fen Chen, Jian Guo Zheng, Changying Hu, Dan Li, Huai Ning Zhong
Summary: The study in China found that the occurrence of mineral oil hydrocarbons (MOH) in food contact papers poses a potential health risk due to the migration of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH). Printed paper had significantly higher migration of MOH compared to unprinted paper, with the highest migration levels observed in packaging papers used for long-term storage.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Chemistry, Applied
Gregory Bauwens, Alexandre Cavaco Soares, Florence Lacoste, Daniel Ribera, Coen Blomsma, Iekje Berg, Fernando Campos, Alwin Coenradie, Adina Creanga, Ralph Zwagerman, Giorgia Purcaro
Summary: The goal of this study was to investigate the impact of refining on the most toxicologically relevant fraction of MOAH contamination in coconut oil. A fully integrated platform consisting of LC, LC-GC x GC, and FID was used to obtain a more detailed characterization of the MOAH sub-classes distribution. The results showed that refining effectively removed naphthalenes and significantly reduced benzo(a)pyrenes.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Plant Sciences
Giuseppe Micalizzi, Yassine Oulad El Majdoub, Filippo Alibrando, Anna Cacciola, Vivienne Spadaro, Luigi Mondello, Maria Paola Germano, Francesco Cacciola
Summary: Anthemis partatoreana, a plant found in the north west coast of Sicily, was analyzed for its chemical composition and antioxidant potential. The most abundant chemical classes in both fresh and dried samples were ketones and esters, with beta-thujone and beta-artemisia acetate as the main compounds. The extracts showed a diverse profile in terms of polyphenolic content, with hydroxycinnamic and flavonoid compounds being the most prominent. The dried methanolic and dried aqueous extracts exhibited the highest quenching ability. However, a slight difference in iron chelating capacity was observed between dried and fresh samples, with the former showing slightly higher power.
Article
Biochemical Research Methods
Katia Arena, Francesco Cacciola, Natalizia Miceli, Maria Fernanda Taviano, Emilia Cavo, Robert E. Murphy, Paola Dugo, Luigi Mondello
Summary: In this study, a comprehensive two-dimensional liquid chromatography system was employed to analyze polyphenolic compounds in commercial berry juices. A total of 104 polyphenolic compounds belonging to different chemical classes were characterized and quantified. Notable variations in polyphenol content were observed among different berry species. The study also determined the total phenolic, flavonoid, and anthocyanin contents, as well as the free-radical scavenging activity and reducing power of the juices. The developed chromatography system showed high separation capability and could be applied to other complex food samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Imad Kabach, Najat Bouchmaa, Reda Ben Mrid, Zakia Zouaoui, Mohammed El Maadoudi, Ayoub Kounnoun, Fadoua Asraoui, Fouad El Mansouri, Abdelmajid Zyad, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Mohamed Nhiri
Summary: This study investigated the phytochemical composition, antioxidant properties, antidiabetic potential, and cytotoxicity of Olea europaea var. oleaster (Oeo) fruit. The results showed that Oeo fruit contains high levels of phenolic and flavonoid compounds and exhibits strong antioxidant activity. Methanolic extract demonstrated better antioxidant activity than aqueous extract and showed inhibitory effects on glycation and breast cancer cells.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Food Science & Technology
Ghanya Al-Naqeb, Cinzia Cafarella, Eugenio Aprea, Giovanna Ferrentino, Alessandra Gasparini, Chiara Buzzanca, Giuseppe Micalizzi, Paola Dugo, Luigi Mondello, Francesca Rigano
Summary: This study evaluated the potential of supercritical fluid extraction (SFE) as a green alternative to Soxhlet extraction for obtaining oils from Opuntia ficus-indica (OFI) and Opuntia dillenii (OD) seeds. SFE with specific parameters produced oils rich in unsaturated fatty acids and tocopherols. The highest yield was achieved through Soxhlet extraction, and the most abundant fatty acid in all samples was linoleic acid. SFE of OFI seeds from Yemen resulted in the highest gamma-tocopherol content. Overall, SFE demonstrated its suitability for producing oils with potential applications in functional food and nutraceutical products.
Article
Food Science & Technology
Marianna Oteri, Giovanni Bartolomeo, Francesca Rigano, Juan Aspromonte, Emanuela Trovato, Giorgia Purcaro, Paola Dugo, Luigi Mondello, Marco Beccaria
Summary: This study comprehensively characterized the chemical components of Chia seed oil, including fatty acid methyl esters (FAMEs), triacylglycerols (TAGs), phospholipids (PLs), free fatty acids (FFAs), sterols, carotenoids, tocopherols, and polyphenols. Different analytical methods such as reversed phase liquid chromatography (RP-LC), gas chromatography (GC), and mass spectrometry (MS) were employed for the analysis of these components. The results showed that Chia seed oil contained high levels of antioxidant tocopherols and essential fatty acids, especially alpha-linolenic acid and linoleic acid. Additionally, this study provided new insights into the composition of FFAs and certain classes of carotenoids in Chia seed oil.
Article
Chemistry, Analytical
Tania Maria Grazia Salerno, Marianna Oteri, Paola Arena, Emanuela Trovato, Danilo Sciarrone, Paola Donato, Luigi Mondello
Summary: Supercritical fluid chromatography (SFC) has gained renewed interest, thanks to advancements in hardware capabilities and tailored packing materials. The use of a subcritical co-solvent mixed with carbon dioxide has expanded the range of SFC-amenable compounds, including lipids. This research developed a subFC method for separating triacylglycerols (TAGs) in various vegetable oils, achieving efficient separations with reduced analysis time and solvent consumption.
Article
Food Science & Technology
Emanuela Trovato, Marina Russo, Lorenzo Cucinotta, Yassine Oulad El Majdoub, Maria Rita Testa Camillo, Gemma De Grazia, Adriana Arigo, Danilo Sciarrone, Luigi Mondello, Paola Dugo
Summary: Flavoured EVOOs in the niche market of extra-virgin olive oil are enriched with aromatic and medicinal plants, which affect their chemical composition. Analytical techniques including HPLC and GC methods were used to investigate the qualiquantitative profile of molecules in the volatile and non-volatile fractions of flavoured EVOOs. The results confirmed the genuineness of most flavoured EVOOs, with a few requiring additional analytical devices for further authentication.
FOOD ANALYTICAL METHODS
(2023)
Article
Chemistry, Applied
Emanuela Trovato, Katia Arena, Roberta La Tella, Francesca Rigano, Roberto Lagana Vinci, Paola Dugo, Luigi Mondello, Paolo Guarnaccia
Summary: This study provides a comprehensive characterization of minor components in hemp seed-based food products, including volatile and non-volatile metabolites. Terpenes were found to be the most abundant compounds among volatiles, while hempseed oils contained 58 compounds in the unsaponifiable matter. Phenols and cannabinoids were also investigated, with a total of 52 compounds identified and quantified.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Katia Arena, Emanuela Trovato, Domenica Mangraviti, Cristina Occhiuto, Francesca Rigano, Francesco Occhiuto, Francesco Cacciola, Luigi Mondello
Summary: This study focuses on the phytochemical characterization and evaluation of the antianginal activity of the bark of Sterculia setigera. Through direct-mass spectrometry analysis, the main constituents of the bark were identified and their correlation with the antianginal activity was determined.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Integrative & Complementary Medicine
Csongor Bako, Viktoria Lilla Balazs, Erika Kerekes, Bela Kocsis, David U. Nagy, Peter Szabo, Giuseppe Micalizzi, Luigi Mondello, Judit Krisch, Dora Petho, Gyorgyi Horvath
Summary: Thyme essential oil is popular due to its antimicrobial effect, but the chemical composition of the oil influences its biological activity. This study investigates the influence of flowering phenophases on the chemical composition, antibacterial and anti-biofilm activity of Thymus vulgaris essential oil, finding that the collection time has important implications for its activity.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Biochemical Research Methods
Alessia Arena, Antonio Ferracane, Mariosimone Zoccali, Markus Obkircher, Hanspeter Sprecher, Peter Q. Tranchida, Luigi Mondello
Summary: This study presents a rapid and limited-volume solvent-extraction sample preparation method for pesticide determination in hemp seed oil. Compared to previous research, this study extends the range of pesticides and investigates a more challenging matrix.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Laura Martin-Pozo, Katia Arena, Francesco Cacciola, Paola Dugo, Luigi Mondello
Summary: Due to concerns over the use of chemicals and pesticides, a comprehensive method using two-dimensional liquid chromatography coupled with tandem mass spectrometry was proposed for the analysis of 112 pesticides in corn-based products. The method employed a reduced QuEChERS-based extraction and clean-up procedure, and achieved low limits of quantification and precision values. Real samples were analyzed and three pesticides were detected at trace levels. This work paves the way for the treatment of complex matrices such as corn products.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Antonio Ferracane, Mariosimone Zoccali, Alessia Arena, Monica Mondello, Peter Q. Tranchida, Luigi Mondello
Summary: The study developed a simplified method for detecting nine phthalic acid esters in extra virgin olive oils using low-pressure gas chromatography-triple-quadrupole mass spectrometry. The method showed high precision and accuracy, with a low limit of quantification for the phthalates. Compared to a previously published method, this approach reduced energy and helium consumption and had shorter analysis times. A total of 23 olive oil samples were analyzed, with almost all samples containing at least one phthalate.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Gemma De Grazia, Lorenzo Cucinotta, Danilo Sciarrone, Paola Donato, Emanuela Trovato, Nacera Riad, Mohamed El Hattab, Luigi Mondello, Archimede Rotondo
Summary: In this study, novel compounds were isolated from brown algae using preparative multidimensional gas chromatography. The correct 3D structures were determined by matching the experimental NMR data with density functional theory simulations. This research provides a new approach for structure elucidation of highly asymmetric molecules.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Applied
Jingfan Wang, Ping Yang, Junmei Liu, Weifang Yang, Yu Qiang, Wei Jia, Dong Han, Chunhui Zhang, Giorgia Purcaro, Marie-Laure Fauconnier
Summary: Soy sauce-based marinade beef (SSMB) is a traditional Chinese cuisine with a unique flavor. Sensory-directed flavor analysis was conducted to identify the key aroma-active compounds in SSMB and their relationship with sensory attributes.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
