Article
Biochemistry & Molecular Biology
Ramazan Kalin, Zeynep Koksal, Songul Bayrak, Serpil Gerni, Isil Nihan Ozyurek, Hande Usanmaz, Muhammet Karaman, Ali Atasever, Hasan Ozdemir, Ilhami Gulcin
Summary: This study tested the inhibition effects of several antibiotics on lactoperoxidase (LPO) and found that cefazolin exhibited the strongest inhibitory efficacy. The study also identified the key role of the 5-methyl-1,3,4-thiadiazol-2-yl moiety in the inhibition mechanism of cefazolin.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Multidisciplinary
Neslihan Macit Kavaz, Deniz Erel, Isil Nihan Korkmaz, Serpil Gerni, Nurgul Abul, Songul Bayrak, Zeynep Koksal, Ramazan Kalin, Aykut Oztekin, Hasan Ozdemir
Summary: A new affinity process using synthesized aminobenzohydrazide derivatives was developed for the purification of Lactoperoxidase. The 4-amino-3-bromo-2-methylbenzohydrazide molecule showed promising results in purifying Lactoperoxidase from mammalian milk. It was demonstrated for the first time that aminobenzohydrazide molecules can be used as ligands for the purification of Lactoperoxidase enzyme.
Article
Biochemistry & Molecular Biology
James O. Larkin, Brianna Jayanthi, Laura Segatori, Zachary T. Ball
Summary: The use of transition-metal-mediated boronic acid chemistry provides a new method for immobilizing proteins on a solid support. This one-step method selectively immobilizes pyroglutamate-histidine (pGH)-tagged proteins. The synthesis of alkenylboronic acid-functionalized poly(ethylene glycol) acrylamide (PEGA) resin and its subsequent reactions with pGH-tagged proteins to form covalent linkages are described in this paper. The selectivity of immobilization is demonstrated through fluorescent studies, model mixtures, and lysates.
Article
Chemistry, Multidisciplinary
Zeynep Koksal
Summary: This study investigated the in vitro effects of some thiophene-2-sulfonamide derivatives on bovine milk LPO enzymatic activity. Among these compounds, 5-(2-thienylthio) thiophene-2-sulfonamide demonstrated the strongest inhibition impact, showing competitive inhibition with an IC50 value of 3.4 nM and a Ki value of 2 +/- 0.6 nM.
DRUG AND CHEMICAL TOXICOLOGY
(2021)
Article
Engineering, Chemical
Nicolas Urtasun, Arn Mignon, Lucas M. Martinez-Alvarez, Maria F. Baieli, Daniela B. Hirsch, Osvaldo Cascone, Peter Dubruel, Federico J. Wolman
Summary: Lactoperoxidase (LP) and lactoferrin (LF) are two proteins in dairy whey with various beneficial properties. Traditional purification methods require pre-treatment steps, but the novel support material CMS-Orange R-HE shows promising adsorption capabilities. Through a one-step adsorption and two-step elution process, LP and LF can be obtained with high yields, purity, and minimal cross-contamination.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yu-ki Tanaka, Shunsuke Shimazaki, Yasunori Fukumoto, Yasumitsu Ogra
Summary: A rapid and precise quantification method for a histidine-tagged recombinant protein produced in Escherichia coli (E. coli) has been developed using single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). This method allows for the determination of protein quantity based on the signal of nickel or cobalt ions bound to the His-tag, without the need for extraction and purification processes.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Wenhao Yin, Xinping Wang, Ying Liao, Lixin Ma, Jie Qiao, Hui Liu, Xin Song, Yi Liu
Summary: The team has established a cost-effective protein purification method, which has been successfully applied in biotransformation. This method not only enables rapid purification with high purity in a single step, but also produces reusable immobilized cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhenlong Guo, YiFei Su, Kexin Li, MengYi Tang, Qiang Li, Shandong Xu
Summary: A octopus-like azobenzene fluorescent probe TPB was synthesized for detecting residual abamectin B-1 in apples, showing reasonable sensitivity, specificity and selectivity.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jui-Chia Chang, Yi-An Chen, Sung-Chyr Lin
Summary: The application of metal chelate-epoxy bifunctional loofah sponge in the selective adsorption and covalent immobilization of His-tagged enzymes was reported. The bifunctional sponge showed high adsorption capacity, superior selectivity, and excellent stability. It can significantly facilitate the enzyme immobilization process and improve the performance compared to other materials.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemistry & Molecular Biology
Cansu Ozturk, Songul Bayrak, Yeliz Demir, Mine Aksoy, Zuhal Alim, Hasan Ozdemir, Omer Irfan Kufrevioglu
Summary: Fresh-cut vegetables and fruits are popular among consumers due to their fresh appearance, lack of pollution, nutrition, and convenience, but enzymatic browning caused by polyphenol oxidase (PPO) is a major issue. Indazoles have been shown to exhibit inhibitory properties against PPO, with 7-chloro-1H-indazole being the most potent inhibitor.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Serpil Gerni, Hasan Ozdemir
Summary: In this study, benzohydroxamic acid molecules were synthesized and the horseradish peroxidase (HRP) enzyme was purified using affinity chromatography technique. The activity of benzohydroxamic acid molecules was found to be influenced by different substituents. The purified HRP enzyme showed optimal activity under specific conditions.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jonas Schilz, Charlotte Clement, Franziska Greiner, Arne Skerra
Summary: A novel strategy for the efficient and gentle affinity purification of PASylated proteins is reported, which allows direct one-step purification from cell extracts or culture supernatants while avoiding harsh elution conditions. This method can improve the efficiency of biological drug development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Anne Chevrel, Leo Candela, Elisa Innocenti, Carolin Golibrzuch, Romas Skudas, Achim Schwaemmle, Manuel J. T. Carrondo, Olivier Kitten, Mikkel Nissum, Ricardo J. S. Silva
Summary: In this study, a novel custom affinity system based on Nanofitin protein scaffold was developed for the purification of biologics beyond antibodies. The system showed excellent performance in purifying a mutant form of streptolysin O, resulting in high purity product and improved process efficiency compared to the traditional three-step process. The custom affinity system has the potential to revolutionize the manufacturing of vaccines and other biological targets.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Nurgul Abul, Serpil Gerni, Isil Nihan Korkmaz, Yeliz Demir, Hasan Ozdemir, Ilhami Gulcin
Summary: Lactoperoxidase enzyme (LPO) is a natural defense mechanism against viruses and bacteria secreted from salivary, mammary, and other mucosal glands. Methyl benzoates were examined for their impact on LPO activity and found to inhibit LPO with Ki values ranging from 0.033±0.004 to 1540.011±460.020 μM. Compound 1a (methyl 2-amino-3-bromobenzoate) showed the strongest inhibition with a Ki value of 0.033±0.004 μM. Docking and MM-GBSA analyses revealed H-bond interactions between the methyl benzoate derivatives and residues Asp108, Ala114, and His351 within the binding cavity.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Armagan Gunsel, Bilge Yazar, Parham Taslimi, Yavuz Erden, Tugba Taskin-Tok, Hasan Piskin, Ahmet T. Bilgicli, M. Nilufer Yarasir, Ilhami Gulcin
Summary: In this study, a novel starting material was synthesized and its structure was verified. Several new phthalocyanine compounds were synthesized and characterized using various spectroscopic methods. These compounds showed inhibitory effects on acetylcholinesterase and alpha-glycosidase enzymes, and exhibited normal cytotoxic effects on cancer cells.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Mustafa Abdullah Yilmaz, Parham Taslimi, Omer Kilic, Ilhami Gulcin, Abhijit Dey, Ercan Bursal
Summary: This ethnobotanical study investigated the chemical constituents and pharmacological activities of Achillea pseudoaleppica. The ethanol and water extracts of A. pseudoaleppica exhibited strong antioxidant properties and inhibited several enzymes. Quinic acid was identified as the most abundant phenolic compound in the extracts. The findings suggest that A. pseudoaleppica has potential therapeutic applications due to its rich phenolic content and pharmacological properties.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Mahmut O. Karaytug, Neslihan Balci, Fikret Turkan, Mahmut Gurbuz, Mehmet E. Demirkol, Zeynep Namli, Lut Tamam, Ilhami Gulcin
Summary: Cholinesterases and glutathione S-transferases (GSTs) play crucial roles in the biochemical processes of the nervous system and detoxification in the human body. Piperazine derivatives show potential therapeutic effects in neurological disorders and cancer research.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Emine O. Karaca, Zeynebe Bingol, Nevin Gurbuz, Ismail Ozdemir, Ilhami Gulcin
Summary: A series of vinyl functionalized 5,6-dimethylbenzimidazolium salts were synthesized and evaluated for their inhibition ability against various enzymes. The compounds showed effective inhibition profiles and may contribute to the development of new drugs for global disorders.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zubeyda Israfilova, Parham Taslimi, Ilhami Gulcin, Yusif Abdullayev, Vagif Farzaliyev, Muhammet Karaman, Afsun Sujayev, Saleh H. Alwasel
Summary: This study focuses on the synthesis of thiocyanate-containing heterocyclic compounds and determines their inhibitory abilities against various enzymes. Theoretical calculations reveal the mechanism of substituting chloride with thiocyanate in aziridine derivatives. The synthesized compounds show potent inhibitory abilities and specifically inhibit hCA I and hCA II enzymes.
Article
Biology
Muzaffer Mutlu, Zeynebe Bingol, Eda Mehtap Uc, Ekrem Koksal, Ahmet C. Goren, Saleh H. Alwasel, Ilhami Gulcin
Summary: This study evaluated the antioxidant and antidiabetic properties of cinnamon leaf essential oil and determined its inhibitory effects on enzymes associated with metabolic diseases. Phenolic contents and essential oil profiles were analyzed. Cinnamon oil showed strong radical scavenging abilities and effectively inhibited carbonic anhydrase II, acetylcholinesterase, and alpha-amylase. This multidisciplinary study is valuable for further research on antioxidant properties and enzyme inhibition profiles of important plants and their oils.
Article
Chemistry, Multidisciplinary
Mehmet Koca, Ilhami Gulcin, Eda Mehtap Uc, Sinan Bilginer, Amine Sena Aydin
Summary: In this study, a hybrid molecule consisting of salicylic acid and salicyl amide structures was synthesized and five new amide derivatives were synthesized for the first time. Their antioxidant potentials were evaluated through various assays, and their potential as drug candidates for Alzheimer's disease was investigated through AChE inhibition studies.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Medicinal
Halil Senol, Gurbet Celik Turgut, Alaattin Sen, Rueya Saglamtas, Salih Tuncay, Ilhami Gulcin, Guelacti Topcu
Summary: In this study, a series of compounds were synthesized from oleanolic acid (OA), including five new compounds (10, 11, 12, 15 and 17) as oxime, imine and hydrazone derivatives. NMR and HRMS analyses were conducted to determine their structures. The inhibitory effects of these compounds on acetylcholinesterase (AChE), human carbonic anhydrase I (hCA I) and II (hCA II) were evaluated. Compounds 13 and 15 exhibited significant inhibitory activity against both hCA I and hCA II, with compound 15 also showing the strongest AChE inhibitory activity.
MEDICINAL CHEMISTRY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hasan Karagecili, Mustafa Abdullah Yilmaz, Adem Erturk, Hatice Kiziltas, Leyla Guven, Saleh H. Alwasel, Ilhami Gulcin
Summary: This study investigated the chemical composition and antioxidant activities of propolis, finding that it is rich in phenolic and flavonoid compounds with antioxidant, antimicrobial, and anticancer properties. Propolis extract also exhibited inhibitory activities against enzymes associated with diabetes, Alzheimer's disease, and glaucoma. Additionally, twenty-eight phenolic compounds were determined to be major organic compounds in propolis, making it a potential natural product for the treatment of various diseases.
Article
Biochemistry & Molecular Biology
Hakan Inci, Ebubekir Izol, Mustafa Abdullah Yilmaz, Mehmet Ilkaya, Zeynebe Bingol, Ilhami Gulcin
Summary: This study evaluated the antioxidant and enzyme inhibition effects of Apilarnil. The antioxidant activity of Apilarnil was lower than standard molecules, but it exhibited higher radical scavenging ability in the DPPH assay. Furthermore, its enzyme inhibition effects were tested against various enzymes. The phytochemical composition of Apilarnil was also determined, with quinic acid, fumaric acid, aconitic acid, kaempferol, and quercetin being the most abundant compounds. Overall, Apilarnil showed effective antioxidant properties compared to standard antioxidants.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Cetin Bayrak, Parham Taslimi, Namik Kilinc, Ilhami Gulcin, Abdullah Menzek
Summary: In this study, the natural bromophenol butyl 2-(3,5-dibromo-4-hydroxyphenyl)acetate (1) and indene derivatives 34 and 35 were successfully synthesized. Several known natural bromophenols and their derivatives were also synthesized. The synthesized compounds exhibited strong inhibition effects against acetylcholinesterase, butyrylcholinesterase, and α-glycosidase enzymes.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Plant Sciences
Mehmet Nuri Atalara, Mine Kokturk, Fikret Altindag, Gunes Ozhan, Tevfik Ozen, Ibrahim Demirtas, Llhami Gulcini
Summary: St. John's wort, a popular medicinal plant, contains bioactive components that are beneficial for health. The extracts showed high survival rates and no significant changes in malformation and larval emergence in zebrafish experiments. However, dosage should be controlled below 10 mg/mL to avoid toxicity.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Biology
Eda Mehtap Ozden, Zeynebe Bingol, Muzaffer Mutlu, Hasan Karagecili, Ekrem Koksal, Ahmet C. Goren, Saleh H. Alwasel, Ilhami Gulcin
Summary: This research comprehensively examined and researched the kiwifruit oil using various bioanalytical methods, determining its antioxidant, antiglaucoma, antidiabetic, and anti-Alzheimer's capabilities. The inhibitory effects on several enzymes linked to metabolic illnesses were also established. The chemical composition and quantity of essential oils contained in kiwifruit oil were studied. The results demonstrated the potent radical scavenging properties and inhibition effects on enzymes of kiwifruit oil, suggesting its significant potential in pharmaceutical procedures.
Article
Biochemistry & Molecular Biology
Alper Yildirim, Ufuk Atmaca, Ertan Sahin, Parham Taslimi, Tugba Taskin-Tok, Murat Celik, Ilhami Gulcin
Summary: This article examines the use of oxazolidinones in various applications such as antibiotics and protein synthesis inhibition. The synthesized compounds were found to have potent inhibitory effects on enzymes such as acetylcholinesterase and carbonic anhydrase. Molecular docking and ADMET analysis were conducted to correlate the experimental data.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Erkan Oner, Yetkin Gok, Yeliz Demir, Tugba Taskin-Tok, Aydin Aktas, Ilhami Gulcin, Serap Yalin
Summary: This study presents the synthesis and characterization of a series of benzimidazolium salts, and investigates their enzyme inhibition abilities against acetylcholinesterase and carbonic anhydrase. The results show that these salts have potent inhibition effects. The pharmacokinetic properties of the compounds were also predicted.
CHEMISTRY & BIODIVERSITY
(2023)
