Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System

Split Cas9, Not Hairs − Advancing the Therapeutic Index of CRISPR Technology

(2018) Carolin Schmelas et al.   Biotechnology Journal

Turning up the Heat with Therapeutic Epigenome Editing

(2018) Alexandra Zezulin et al.   Cell Stem Cell

Epigenetics as biomarkers in autoimmune diseases

(2018) Haijing Wu et al.   CLINICAL IMMUNOLOGY

Seamless Genetic Conversion of SMN2 to SMN1 via CRISPR/Cpf1 and Single-Stranded Oligodeoxynucleotides in Spinal Muscular Atrophy Patient-Specific Induced Pluripotent Stem Cells

(2018) Miaojin Zhou et al.   HUMAN GENE THERAPY

Split Cas9, Not Hairs − Advancing the Therapeutic Index of CRISPR Technology

(2018) Carolin Schmelas et al.   Biotechnology Journal

Identification of physical interactions between genomic regions by enChIP-Seq

(2017) Toshitsugu Fujita et al.   GENES TO CELLS

Genome and Epigenome Editing to Treat Disorders of the Hematopoietic System

(2017) Claudio Mussolino et al.   HUMAN GENE THERAPY

CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter

(2017) Sergio Covarrubias et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR Epigenome Editing of AKAP150 in DRG Neurons Abolishes Degenerative IVD-Induced Neuronal Activation

(2017) Joshua D. Stover et al.   MOLECULAR THERAPY

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome

(2017) Tyler S Klann et al.   NATURE BIOTECHNOLOGY

Epigenetics of cell fate reprogramming and its implications for neurological disorders modelling

(2017) Maciej Grzybek et al.   NEUROBIOLOGY OF DISEASE

Cell-type-specific genome editing with a microRNA-responsive CRISPR–Cas9 switch

(2017) Moe Hirosawa et al.   NUCLEIC ACIDS RESEARCH

Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells

(2017) Masahiro Okada et al.   Epigenetics & Chromatin

Rapid and reversible epigenome editing by endogenous chromatin regulators

(2017) Simon M. G. Braun et al.   Nature Communications

Applications of CRISPR/Cas9 in retinal degenerative diseases

(2017)   International Journal of Ophthalmology

DNA methylation/hydroxymethylation in melanoma

(2017) Siqi Fu et al.   Oncotarget

Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics

(2017) Chiao-Ling Lo et al.   Scientific Reports

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

CRISPR/Cas9 for Human Genome Engineering and Disease Research

(2016) Xin Xiong et al.   Annual Review of Genomics and Human Genetics

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing

(2016) Angelo Amabile et al.   CELL

In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming

(2016) Alejandro Ocampo et al.   CELL

Optimized inducible shRNA and CRISPR/Cas9 platforms forin vitrostudies of human development using hPSCs

(2016) Alessandro Bertero et al.   DEVELOPMENT

Epigenetic Treatment of Persistent Viral Infections

(2016) Walter H. Moos et al.   DRUG DEVELOPMENT RESEARCH

Genome and Epigenome Editing in Mechanistic Studies of Human Aging and Aging-Related Disease

(2016) Cia-Hin Lau et al.   GERONTOLOGY

Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions

(2016) Sumiyo Morita et al.   NATURE BIOTECHNOLOGY

Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9

(2016) Gozde Korkmaz et al.   NATURE BIOTECHNOLOGY

A multifunctional AAV–CRISPR–Cas9 and its host response

(2016) Wei Leong Chew et al.   NATURE METHODS

In vivo high-throughput profiling of CRISPR–Cpf1 activity

(2016) Hui K Kim et al.   NATURE METHODS

From profiles to function in epigenomics

(2016) Stefan H. Stricker et al.   NATURE REVIEWS GENETICS

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Expanding the CRISPR imaging toolset withStaphylococcus aureusCas9 for simultaneous imaging of multiple genomic loci

(2016) Baohui Chen et al.   NUCLEIC ACIDS RESEARCH

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

(2016) Yi Fu et al.   Nature Communications

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

(2016) Duy P. Nguyen et al.   Nature Communications

Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system

(2016) Benjamin Garcia-Bloj et al.   Oncotarget

Optogenetic regulation of site-specific subtelomeric DNA methylation

(2016) Samrat Roy Choudhury et al.   Oncotarget

In vivo reprogramming reactive glia into iPSCs to produce new neurons in the cortex following traumatic brain injury

(2016) Xiang Gao et al.   Scientific Reports

In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system

(2016) Anand Narayanan et al.   Scientific Reports

The Application of Human iPSCs in Neurological Diseases: From Bench to Bedside

(2016) Nina Xie et al.   Stem Cells International

Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation

(2016) James I. McDonald et al.   Biology Open

Precision Medicine: Genetic Repair of Retinitis Pigmentosa in Patient-Derived Stem Cells

(2016) Alexander G. Bassuk et al.   Scientific Reports

CRISPR/cas9, a novel genomic tool to knock down microRNA in vitro and in vivo

(2016) Hong Chang et al.   Scientific Reports

DNA methylation and hydroxymethylation in stem cells

(2015) Ying Cheng et al.   CELL BIOCHEMISTRY AND FUNCTION

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Functional annotation of native enhancers with a Cas9–histone demethylase fusion

(2015) Nicola A Kearns et al.   NATURE METHODS

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Rapid reverse genetic screening using CRISPR in zebrafish

(2015) Arish N Shah et al.   NATURE METHODS

Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

(2015) Pratiksha I Thakore et al.   NATURE METHODS

Amyotrophic lateral sclerosis: mechanisms and therapeutics in the epigenomic era

(2015) Ximena Paez-Colasante et al.   Nature Reviews Neurology

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

Multicolor CRISPR labeling of chromosomal loci in human cells

(2015) Hanhui Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rational design of a split-Cas9 enzyme complex

(2015) Addison V. Wright et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained

(2015) Goran Kungulovski et al.   Epigenetics & Chromatin

Epigenetics and Triplet-Repeat Neurological Diseases

(2015) Sathiji Nageshwaran et al.   Frontiers in Neurology

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors

(2014) Elizabeth A Heller et al.   NATURE NEUROSCIENCE

Direct activation of human and mouse Oct4 genes using engineered TALE and Cas9 transcription factors

(2014) Jiabiao Hu et al.   NUCLEIC ACIDS RESEARCH

Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines

(2014) Tsui-Ting Ho et al.   NUCLEIC ACIDS RESEARCH

Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9

(2014) Jinxiong Han et al.   RNA Biology

Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA

(2014) C. Long et al.   SCIENCE

Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR

(2013) Toshitsugu Fujita et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

Programming genomes with light

(2013) Andreas Möglich et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Optical control of mammalian endogenous transcription and epigenetic states

(2013) Silvana Konermann et al.   NATURE

Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins

(2013) Morgan L Maeder et al.   NATURE BIOTECHNOLOGY

Epigenome editing

(2013) Philipp Voigt et al.   NATURE BIOTECHNOLOGY

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

Structure and function of long noncoding RNAs in epigenetic regulation

(2013) Tim R Mercer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish

(2013) An Xiao et al.   NUCLEIC ACIDS RESEARCH

In Vivo Cell Reprogramming towards Pluripotency by Virus-Free Overexpression of Defined Factors

(2013) Açelya Yilmazer et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

An ex vivo gene therapy approach to treat muscular dystrophy using inducible pluripotent stem cells

(2013) Antonio Filareto et al.   Nature Communications

Toward pluripotency by reprogramming: mechanisms and application

(2013) Tao Wang et al.   Protein & Cell

An optimized two-finger archive for ZFN-mediated gene targeting

(2012) Ankit Gupta et al.   NATURE METHODS

Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors

(2012) D. Deng et al.   SCIENCE

State-of-the-art gene-based therapies: the road ahead

(2011) Mark A. Kay   NATURE REVIEWS GENETICS

Origins and Mechanisms of miRNAs and siRNAs

(2009) Richard W. Carthew et al.   CELL

Mechanisms of imprinting of the Prader-Willi/Angelman region

(2008) Bernhard Horsthemke et al.   AMERICAN JOURNAL OF MEDICAL GENETICS PART A