Optimized inducible shRNA and CRISPR/Cas9 platforms forin vitrostudies of human development using hPSCs

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

(2016) Mohammad A. Mandegar et al.   Cell Stem Cell

Initiation of stem cell differentiation involves cell cycle-dependent regulation of developmental genes by Cyclin D

(2016) Siim Pauklin et al.   GENES & DEVELOPMENT

Pluripotent stem cells in disease modelling and drug discovery

(2016) Yishai Avior et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Factors that coordinate mesoderm specification from neuromesodermal progenitors with segmentation during vertebrate axial extension

(2016) Benjamin L. Martin   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

(2015) Yuejun Chen et al.   Cell Stem Cell

Brachyury and SMAD signalling collaboratively orchestrate distinct mesoderm and endoderm gene regulatory networks in differentiating human embryonic stem cells

(2015) T. Faial et al.   DEVELOPMENT

Activin/Nodal signaling and NANOG orchestrate human embryonic stem cell fate decisions by controlling the H3K4me3 chromatin mark

(2015) Alessandro Bertero et al.   GENES & DEVELOPMENT

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

(2015) Antonia A. Dominguez et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition

(2015) Laura Ordovás et al.   Stem Cell Reports

The DPY30 subunit in SET1/MLL complexes regulates the proliferation and differentiation of hematopoietic progenitor cells

(2014) Z. Yang et al.   BLOOD

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

NANOG and CDX2 Pattern Distinct Subtypes of Human Mesoderm during Exit from Pluripotency

(2014) Sasha Mendjan et al.   Cell Stem Cell

An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells

(2014) Federico González et al.   Cell Stem Cell

Looking inwards: opening a window onto human development

(2014) O. Pourquie et al.   DEVELOPMENT

The T-box gene family: emerging roles in development, stem cells and cancer

(2014) V. E. Papaioannou   DEVELOPMENT

The Cell-Cycle State of Stem Cells Determines Cell Fate Propensity

(2013) Siim Pauklin et al.   CELL

Human pluripotent stem cells: an emerging model in developmental biology

(2013) Z. Zhu et al.   DEVELOPMENT

Efficient ROSA26-Based Conditional and/or Inducible Transgenesis Using RMCE-Compatible F1 Hybrid Mouse Embryonic Stem Cells

(2013) Lieven Haenebalcke et al.   Stem Cell Reviews and Reports

Distinct Lineage Specification Roles for NANOG, OCT4, and SOX2 in Human Embryonic Stem Cells

(2012) Zheng Wang et al.   Cell Stem Cell

Extensive Methylation of Promoter Sequences Silences Lentiviral Transgene Expression During Stem Cell Differentiation In Vivo

(2012) Friederike Herbst et al.   MOLECULAR THERAPY

An RNA interference screen uncovers a new molecule in stem cell self-renewal and long-term regeneration

(2012) Ting Chen et al.   NATURE

Generation of human vascular smooth muscle subtypes provides insight into embryological origin–dependent disease susceptibility

(2012) Christine Cheung et al.   NATURE BIOTECHNOLOGY

Therapeutic opportunities for manipulating TReg cells in autoimmunity and cancer

(2012) Harald von Boehmer et al.   NATURE REVIEWS DRUG DISCOVERY

Induction of MesP1 by Brachyury(T) generates the common multipotent cardiovascular stem cell

(2011) Robert David et al.   CARDIOVASCULAR RESEARCH

Pluripotency Factors in Embryonic Stem Cells Regulate Differentiation into Germ Layers

(2011) Matt Thomson et al.   CELL

Role for Dpy-30 in ES Cell-Fate Specification by Regulation of H3K4 Methylation within Bivalent Domains

(2011) Hao Jiang et al.   CELL

BRACHYURY and CDX2 Mediate BMP-Induced Differentiation of Human and Mouse Pluripotent Stem Cells into Embryonic and Extraembryonic Lineages

(2011) Andreia S. Bernardo et al.   Cell Stem Cell

Needles in stacks of needles: finding disease-causal variants in a wealth of genomic data

(2011) Gregory M. Cooper et al.   NATURE REVIEWS GENETICS

Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors

(2011) Chrishan J. A. Ramachandra et al.   NUCLEIC ACIDS RESEARCH

Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression

(2011) Stephan Fath et al.   PLoS One

Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome

(2010) R. C. DeKelver et al.   GENOME RESEARCH

Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells

(2010) S. Tamir Rashid et al.   JOURNAL OF CLINICAL INVESTIGATION

Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter

(2010) Jane Yuxia Qin et al.   PLoS One

MicroRNA sponges: Progress and possibilities

(2010) M. S. Ebert et al.   RNA

Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development

(2009) Thomas Touboul et al.   HEPATOLOGY

Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells

(2009) Ángel Raya et al.   NATURE

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Specific Knockdown of OCT4 in Human Embryonic Stem Cells by Inducible Short Hairpin RNA Interference

(2009) Gaetano Zafarana et al.   STEM CELLS