Engineering terpenoid production through transient expression in Nicotiana benthamiana

A translational synthetic biology platform for rapid access to gram-scale quantities of novel drug-like molecules

(2017) James Reed et al.   METABOLIC ENGINEERING

Unearthing a sesterterpene biosynthetic repertoire in the Brassicaceae through genome mining reveals convergent evolution

(2017) Ancheng C. Huang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant scientists plan massive effort to sequence 10,000 genomes

(2017) Dennis Normile   SCIENCE

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis

(2016) Johan Andersen-Ranberg et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Molecular pharming — VLPs made in plants

(2016) Johanna Marsian et al.   CURRENT OPINION IN BIOTECHNOLOGY

Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua

(2016) Bo Wang et al.   METABOLIC ENGINEERING

Computational genomic identification and functional reconstitution of plant natural product biosynthetic pathways

(2016) Marnix H. Medema et al.   NATURAL PRODUCT REPORTS

Plant metabolic clusters - from genetics to genomics

(2016) Hans-Wilhelm Nützmann et al.   NEW PHYTOLOGIST

Co-expression of peppermint geranyl diphosphate synthase small subunit enhances monoterpene production in transgenic tobacco plants

(2016) Jun-Lin Yin et al.   NEW PHYTOLOGIST

Engineering biosynthesis of the anticancer alkaloid noscapine in yeast

(2016) Yanran Li et al.   Nature Communications

Discovery and resupply of pharmacologically active plant-derived natural products: A review

(2015) Atanas G. Atanasov et al.   BIOTECHNOLOGY ADVANCES

The increasing value of plant-made proteins

(2015) Markus Sack et al.   CURRENT OPINION IN BIOTECHNOLOGY

SNARE-RNAi Results in Higher Terpene Emission from Ectopically Expressed Caryophyllene Synthase in Nicotiana benthamiana

(2015) Hieng-Ming Ting et al.   Molecular Plant

A new cyanogenic metabolite in Arabidopsis required for inducible pathogen defence

(2015) Jakub Rajniak et al.   NATURE

Monoterpene biosynthesis potential of plant subcellular compartments

(2015) Lemeng Dong et al.   NEW PHYTOLOGIST

Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants

(2015) Guy Polturak et al.   NEW PHYTOLOGIST

P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins

(2015) Hikaru Seki et al.   PLANT AND CELL PHYSIOLOGY

Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals

(2015) Barry R. Holtz et al.   PLANT BIOTECHNOLOGY JOURNAL

A library of synthetic transcription activator-like effector-activated promoters for coordinated orthogonal gene expression in plants

(2015) Kathleen Brückner et al.   PLANT JOURNAL

Identification and genome organization of saponin pathway genes from a wild crucifer, and their use for transient production of saponins inNicotiana benthamiana

(2015) Bekzod Khakimov et al.   PLANT JOURNAL

De novo production of the plant-derived alkaloid strictosidine in yeast

(2015) Stephanie Brown et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone

(2015) Warren Lau et al.   SCIENCE

Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

(2015) Philipp Zerbe et al.   TRENDS IN BIOTECHNOLOGY

Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

(2015) Thiyagarajan Gnanasekaran et al.   Journal of Biological Engineering

(+)-Valencene production inNicotiana benthamianais increased by down-regulation of competing pathways

(2014) Katarina Cankar et al.   Biotechnology Journal

Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway

(2014) Qing Liu et al.   METABOLIC ENGINEERING

Large-scale de novo DNA synthesis: technologies and applications

(2014) Sriram Kosuri et al.   NATURE METHODS

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene

(2014) Md. Mohidul Hasan et al.   PLANT CELL REPORTS

Comparison of plant-based expression platforms for the heterologous production of geraniol

(2014) Nikolay Vasilev et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway

(2014) Kaouthar Eljounaidi et al.   PLANT SCIENCE

Investigation of terpene diversification across multiple sequenced plant genomes

(2014) Alexander M. Boutanaev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The seco-iridoid pathway from Catharanthus roseus

(2014) Karel Miettinen et al.   Nature Communications

A Golden Gate Modular Cloning Toolbox for Plants

(2014) Carola Engler et al.   ACS Synthetic Biology

Data access for the 1,000 Plants (1KP) project

(2014) Naim Matasci et al.   GigaScience

Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis

(2013) Eva Vranová et al.   Annual Review of Plant Biology

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

A metabolic gene cluster inLotus japonicusdiscloses novel enzyme functions and products in triterpene biosynthesis

(2013) Afrodite Krokida et al.   NEW PHYTOLOGIST

Plant P450s as versatile drivers for evolution of species-specific chemical diversity

(2013) B. Hamberger et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles

(2013) Eva C. Thuenemann et al.   PLANT BIOTECHNOLOGY JOURNAL

High-level diterpene production by transient expression in Nicotiana benthamiana

(2013) Kathleen Brückner et al.   Plant Methods

Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants

(2013) K. Geisler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems

(2012) Marc J.C. Fischer et al.   JOURNAL OF BIOTECHNOLOGY

Engineering triterpene metabolism in tobacco

(2012) Shuiqin Wu et al.   PLANTA

Recent advances of Cowpea mosaic virus-based particle technology

(2011) Nicholas P. Montague et al.   Human vaccines & immunotherapeutics

Generation of the potent anti-malarial drug artemisinin in tobacco

(2011) Moran Farhi et al.   NATURE BIOTECHNOLOGY

Metabolic Engineering inNicotiana benthamianaReveals Key Enzyme Functions inArabidopsisIndole Glucosinolate Modification

(2011) Marina Pfalz et al.   PLANT CELL

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Reconstitution of the Costunolide Biosynthetic Pathway in Yeast and Nicotiana benthamiana

(2011) Qing Liu et al.   PLoS One

A raison d’être for two distinct pathways in the early steps of plant isoprenoid biosynthesis?

(2011) Andréa Hemmerlin et al.   PROGRESS IN LIPID RESEARCH

Nicotiana benthamiana as a Production Platform for Artemisinin Precursors

(2010) Teun W. J. M. van Herpen et al.   PLoS One

Glucosinolate engineering identifies a γ-glutamyl peptidase

(2009) Fernando Geu-Flores et al.   Nature Chemical Biology