Biosynthesis and incorporation of an alkylproline-derivative (APD) precursor into complex natural products

A tricyclic pyrrolobenzodiazepine produced byKlebsiella oxytocais associated with cytotoxicity in antibiotic-associated hemorrhagic colitis

(2017) Herman Tse et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Exploring the biocombinatorial potential of benzoxazoles: generation of novel caboxamycin derivatives

(2017) Armando A. Losada et al.   Microbial Cell Factories

1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life

(2017) Supratim Mukherjee et al.   NATURE BIOTECHNOLOGY

Evolution-guided adaptation of an adenylation domain substrate specificity to an unusual amino acid

(2017) Simon Vobruba et al.   PLoS One

Elucidation of salicylate attachment in celesticetin biosynthesis opens the door to create a library of more efficient hybrid lincosamide antibiotics

(2017) S. Kadlcik et al.   Chemical Science

4-alkyl-L-(Dehydro)proline biosynthesis in actinobacteria involves N-terminal nucleophile-hydrolase activity of γ-glutamyltranspeptidase homolog for C-C bond cleavage

(2017) Guannan Zhong et al.   Nature Communications

From Anthramycin to Pyrrolobenzodiazepine (PBD)-Containing Antibody-Drug Conjugates (ADCs)

(2016) Julia Mantaj et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Activator of an Adenylation Domain Revealed by Activity but Not Sequence Homology

(2016) Shalini Saha et al.   CHEMBIOCHEM

Characterization of Enzymes Catalyzing Transformations of CysteineS-Conjugated Intermediates in the Lincosamide Biosynthetic Pathway

(2016) Richiro Ushimaru et al.   CHEMBIOCHEM

Linking Biosynthetic Gene Clusters to their Metabolites via Pathway- Targeted Molecular Networking

(2016) Eric P. Trautman et al.   CURRENT TOPICS IN MEDICINAL CHEMISTRY

Design and synthesis of pyrrolobenzodiazepine-gallic hybrid agents as p53-dependent and -independent apoptogenic signaling in melanoma cells

(2016) Yu-Wei Chou et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Differences in PLP-Dependent Cysteinyl Processing Lead to Diverse S-Functionalization of Lincosamide Antibiotics

(2016) Min Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis of coral settlement cue tetrabromopyrrole in marine bacteria by a uniquely adapted brominase–thioesterase enzyme pair

(2016) Abrahim El Gamal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Deacetylation of mycothiol-derived ‘waste product’ triggers the last biosynthetic steps of lincosamide antibiotics

(2016) Zdenek Kamenik et al.   Chemical Science

New Concept of the Biosynthesis of 4-Alkyl-L-Proline Precursors of Lincomycin, Hormaomycin, and Pyrrolobenzodiazepines: Could a γ-Glutamyltransferase Cleave the C–C Bond?

(2016) Petra Jiraskova et al.   Frontiers in Microbiology

The versatile low-molecular-weight thiols: Beyond cell protection

(2015) Min Wang et al.   BIOESSAYS

Identification of the dioxygenase-generated intermediate formed during biosynthesis of the dihydropyrrole moiety common to anthramycin and sibiromycin

(2015) Shalini Saha et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Synthetic modified pyrrolo[1,4] benzodiazepine molecules demonstrate selective anticancer activity by targeting the human ligase 1 enzyme: An in silico and in vitro mechanistic study

(2015) Mohammad Shameem et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Characterization of the Biosynthetic Gene Cluster for Benzoxazole Antibiotics A33853 Reveals Unusual Assembly Logic

(2015) Meinan Lv et al.   CHEMISTRY & BIOLOGY

Co-overexpression oflmbWandmetKled to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain ofStreptomyces lincolnensis

(2015) A.-P. Pang et al.   JOURNAL OF APPLIED MICROBIOLOGY

Hormaomycins B and C: New Antibiotic Cyclic Depsipeptides from a Marine Mudflat-Derived Streptomyces sp.

(2015) Munhyung Bae et al.   Marine Drugs

Characterization of Cichopeptins, New Phytotoxic Cyclic Lipodepsipeptides Produced byPseudomonas cichoriiSF1-54 and Their Role in Bacterial Midrib Rot Disease of Lettuce

(2015) Chien-Jui Huang et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

Metabolic coupling of two small-molecule thiols programs the biosynthesis of lincomycin A

(2015) Qunfei Zhao et al.   NATURE

Spithioneines A and B, Two New Bohemamine Derivatives Possessing Ergothioneine Moiety from a Marine-Derived Streptomyces spinoverrucosus

(2015) Peng Fu et al.   ORGANIC LETTERS

Lincosamide Synthetase—A Unique Condensation System Combining Elements of Nonribosomal Peptide Synthetase and Mycothiol Metabolism

(2015) Jiri Janata et al.   PLoS One

Identification and Analysis of the Paulomycin Biosynthetic Gene Cluster and Titer Improvement of the Paulomycins in Streptomyces paulus NRRL 8115

(2015) Jine Li et al.   PLoS One

4-Methylproline Guided Natural Product Discovery: Co-Occurrence of 4-Hydroxy- and 4-Methylprolines in Nostoweipeptins and Nostopeptolides

(2014) Liwei Liu et al.   ACS Chemical Biology

Boseongazepines A–C, pyrrolobenzodiazepine derivatives from a Streptomyces sp. 11A057

(2014) Mijin Oh et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Insights into Secondary Metabolism from a Global Analysis of Prokaryotic Biosynthetic Gene Clusters

(2014) Peter Cimermancic et al.   CELL

Sequence analysis of porothramycin biosynthetic gene cluster

(2014) Lucie Najmanova et al.   FOLIA MICROBIOLOGICA

In Vitro Characterization of LmbK and LmbO: Identification of GDP-d-erythro-α-d-gluco-octose as a Key Intermediate in Lincomycin A Biosynthesis

(2014) Chia-I Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Elucidation of Final Steps of the Marineosins Biosynthetic Pathway through Identification and Characterization of the Corresponding Gene Cluster

(2014) Shaimaa M. Salem et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Hybrid Non-Ribosomal Peptide/Polyketide Synthetase Containing Fatty-Acyl Ligase (FAAL) Synthesizes the β-Amino Fatty Acid Lipopeptides Puwainaphycins in the Cyanobacterium Cylindrospermum alatosporum

(2014) Jan Mareš et al.   PLoS One

Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis

(2014) G. Schneditz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Systematic Computational Analysis of Biosynthetic Gene Cluster Evolution: Lessons for Engineering Biosynthesis

(2014) Marnix H. Medema et al.   PLoS Computational Biology

Structure and possible mechanism of the CcbJ methyltransferase fromStreptomyces caelestis

(2014) Jacob Bauer et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure and mechanism of a nonhaem-iron SAM-dependentC-methyltransferase and its engineering to a hydratase and anO-methyltransferase

(2014) Xiao-Wei Zou et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Characterization ofN-Demethyllincosamide Methyltransferases LmbJ and CcbJ

(2013) Lucie Najmanová et al.   CHEMBIOCHEM

Manipulation of Regulatory Genes Reveals Complexity and Fidelity in Hormaomycin Biosynthesis

(2013) Xiaofeng Cai et al.   CHEMISTRY & BIOLOGY

Neuraminidase Inhibitors from marine-derived actinomyceteStreptomyces seoulensis

(2013) R.H. Jiao et al.   JOURNAL OF APPLIED MICROBIOLOGY

Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus

(2013) Xiaoling Wang et al.   Molecular BioSystems

Ribosome-targeting antibiotics and mechanisms of bacterial resistance

(2013) Daniel N. Wilson   NATURE REVIEWS MICROBIOLOGY

Lincomycin Biosynthesis Involves a Tyrosine Hydroxylating Heme Protein of an Unusual Enzyme Family

(2013) Jitka Novotna et al.   PLoS One

Adaptation of an L-Proline Adenylation Domain to Use 4-Propyl-L-Proline in the Evolution of Lincosamide Biosynthesis

(2013) Stanislav Kadlčík et al.   PLoS One

Low-Molecular-Weight Thiols in Thiol–Disulfide Exchange

(2012) Koen Van Laer et al.   ANTIOXIDANTS & REDOX SIGNALING

Glutathione analogs in prokaryotes

(2012) Robert C. Fahey   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Construction of the Octose 8-Phosphate Intermediate in Lincomycin A Biosynthesis: Characterization of the Reactions Catalyzed by LmbR and LmbN

(2012) Eita Sasaki et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A stand-alone adenylation domain forms amide bonds in streptothricin biosynthesis

(2012) Chitose Maruyama et al.   Nature Chemical Biology

Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains

(2012) Max Crüsemann et al.   Chemical Science

Anatoxin-a Synthetase Gene Cluster of the Cyanobacterium Anabaena sp. Strain 37 and Molecular Methods To Detect Potential Producers

(2011) Anne Rantala-Ylinen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biosynthesis of the nargenicin A1 pyrrole moiety from Nocardia sp. CS682

(2011) Sushila Maharjan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biosynthesis and function of gliotoxin in Aspergillus fumigatus

(2011) Daniel H. Scharf et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Four-Enzyme Pathway for 3,5-Dihydroxy-4-methylanthranilic Acid Formation and Incorporation into the Antitumor Antibiotic Sibiromycin

(2011) Tobias W. Giessen et al.   BIOCHEMISTRY

A Heme Peroxidase with a Functional Role as anl-Tyrosine Hydroxylase in the Biosynthesis of Anthramycin

(2011) Katherine L. Connor et al.   BIOCHEMISTRY

Kinetic, Crystallographic, and Mechanistic Characterization of TomN: Elucidation of a Function for a 4-Oxalocrotonate Tautomerase Homologue in the Tomaymycin Biosynthetic Pathway

(2011) Elizabeth A. Burks et al.   BIOCHEMISTRY

Usabamycins A–C: New anthramycin-type analogues from a marine-derived actinomycete

(2011) Seizo Sato et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Elegant Metabolite Biosynthesis

(2011) Adam C. Jones et al.   CHEMISTRY & BIOLOGY

Insights into the Biosynthesis of Hormaomycin, An Exceptionally Complex Bacterial Signaling Metabolite

(2011) Ivonne Höfer et al.   CHEMISTRY & BIOLOGY

Molecular cloning and identification of the laspartomycin biosynthetic gene cluster from Streptomyces viridochromogenes

(2011) Yang Wang et al.   GENE

Two novel benzastatin derivatives, JBIR-67 and JBIR-73, isolated from Streptomyces sp. RI18

(2011) Keiichiro Motohashi et al.   JOURNAL OF ANTIBIOTICS

Ligand Binding Induces an Ammonia Channel in 2-Amino-2-desoxyisochorismate (ADIC) Synthase PhzE

(2011) Qi-Ang Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biosynthetic Gene Cluster of the Non-ribosomally Synthesized Cyclodepsipeptide Skyllamycin: Deciphering Unprecedented Ways of Unusual Hydroxylation Reactions

(2011) Stefan Pohle et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Insights into the complex biosynthesis of the leupyrrins in Sorangium cellulosum So ce690

(2011) Maren Kopp et al.   Molecular BioSystems

Aminobenzoates as building blocks for natural productassembly lines

(2011) Christopher T. Walsh et al.   NATURAL PRODUCT REPORTS

A DC-81-indole conjugate agent suppresses melanoma A375 cell migration partially via interrupting VEGF production and stromal cell-derived factor-1α-mediated signaling

(2011) Ming-Chu Hsieh et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Characterization of the Biosynthesis Gene Cluster for the Pyrrole Polyether Antibiotic Calcimycin (A23187) inStreptomyces chartreusisNRRL 3882

(2010) Qiulin Wu et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Identification of the Biosynthetic Gene Cluster for 3-Methylarginine, a Toxin Produced by Pseudomonas syringae pv. syringae 22d/93

(2010) S. D. Braun et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Pharmacokinetics, pharmacodynamics and metabolism of the dimeric pyrrolobenzodiazepine SJG-136 in rats

(2010) Joel M. Reid et al.   CANCER CHEMOTHERAPY AND PHARMACOLOGY

Determination of sibiromycin and its natural derivatives using new analytical and structural approaches

(2010) Miroslav Šulc et al.   JOURNAL OF CHROMATOGRAPHY A

Biosynthesis, synthesis, and biological activities of pyrrolobenzodiazepines

(2010) Barbara Gerratana   MEDICINAL RESEARCH REVIEWS

Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action

(2010) J. A. Dunkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli

(2010) A. Gaballa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mutasynthesis of Lincomycin Derivatives with Activity against Drug-Resistant Staphylococci

(2009) D. Ulanova et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Cloning and Characterization of the Biosynthetic Gene Cluster for Tomaymycin, an SJG-136 Monomeric Analog

(2009) W. Li et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biosynthesis of Sibiromycin, a Potent Antitumor Antibiotic

(2009) W. Li et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

In VitroReconstitution of the First Steps of Anatoxin-a Biosynthesis inOscillatoriaPCC 6506: From Freel-Proline to Acyl Carrier Protein Bound Dehydroproline

(2009) Annick Méjean et al.   BIOCHEMISTRY

In vitro Characterization of Enzymes Involved in the Synthesis of Nonproteinogenic Residue (2S,3S)-β-Methylphenylalanine in Glycopeptide Antibiotic Mannopeptimycin

(2009) Yu-Ting Huang et al.   CHEMBIOCHEM

Analysis of the Indanomycin Biosynthetic Gene Cluster fromStreptomyces antibioticusNRRL 8167

(2009) Chaoxuan Li et al.   CHEMBIOCHEM

Adenylation Enzyme Characterization Using γ -18O4-ATP Pyrophosphate Exchange

(2009) Vanessa V. Phelan et al.   CHEMISTRY & BIOLOGY

Prodigiosin biosynthesis gene cluster in the roseophilin producer Streptomyces griseoviridis

(2009) Takashi Kawasaki et al.   JOURNAL OF ANTIBIOTICS

Limazepines A−F, Pyrrolo[1,4]benzodiazepine Antibiotics from an Indonesian Micrococcus sp.

(2009) Serge Fotso et al.   JOURNAL OF NATURAL PRODUCTS

Biochemical characterization of l-DOPA 2,3-dioxygenase, a single-domain type I extradiol dioxygenase from lincomycin biosynthesis

(2008) Keri L. Colabroy et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

novE and novG act as positive regulators of novobiocin biosynthesis

(2008) Volker Dangel et al.   ARCHIVES OF MICROBIOLOGY

Use of a Halogenase of Hormaomycin Biosynthesis for Formation of New Clorobiocin Analogues with 5-Chloropyrrole Moieties

(2008) Lutz Heide et al.   CHEMBIOCHEM

DKxanthene Biosynthesis—Understanding the Basis for Diversity-Oriented Synthesis in Myxobacterial Secondary Metabolism

(2008) Peter Meiser et al.   CHEMISTRY & BIOLOGY

Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

(2008) M. Koběrská et al.   FOLIA MICROBIOLOGICA

Biosynthesis of Diazepinomicin/ECO-4601, aMicromonosporaSecondary Metabolite with a Novel Ring System

(2008) James B. McAlpine et al.   JOURNAL OF NATURAL PRODUCTS

Isolation, Structure, and Antibacterial Activities of Lucensimycins D−G, Discovered fromStreptomyces lucensisMA7349 Using an Antisense Strategy⊥

(2008) Sheo B. Singh et al.   JOURNAL OF NATURAL PRODUCTS

Biosynthesis and Functions of Mycothiol, the Unique Protective Thiol of Actinobacteria

(2008) G. L. Newton et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78

(2008) M. Kotecha et al.   MOLECULAR CANCER THERAPEUTICS

Mycothiol: synthesis, biosynthesis and biological functions of the major low molecular weight thiol in actinomycetes

(2008) Vishnu Karthik Jothivasan et al.   NATURAL PRODUCT REPORTS

Four enzymes define the incorporation of coenzyme A in thienamycin biosynthesis

(2008) M. F. Freeman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA