Biochemical and biophysical characterization of humanized IgG1 produced inPichia pastoris

Isolation and characterization of IgG1 with asymmetrical Fc glycosylation

(2011) S. Ha et al.   GLYCOBIOLOGY

Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study

(2011) Ningyan Zhang et al.   mAbs

Impact of methionine oxidation in human IgG1 Fc on serum half-life of monoclonal antibodies

(2011) Weirong Wang et al.   MOLECULAR IMMUNOLOGY

Separation of post-translational modifications in monoclonal antibodies by exploiting subtle conformational changes under mildly acidic conditions

(2010) Shiyi Wang et al.   JOURNAL OF CHROMATOGRAPHY A

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

(2010) Gavin C. Barnard et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris

(2010) Youwei Jiang et al.   PROTEIN EXPRESSION AND PURIFICATION

Identification and characterization of asparagine deamidation in the light chain CDR1 of a humanized IgG1 antibody

(2009) Josef Vlasak et al.   ANALYTICAL BIOCHEMISTRY

Investigation of Sample Preparation Artifacts Formed during the Enzymatic Release of N-Linked Glycans prior to Analysis by Capillary Electrophoresis

(2009) Yong Liu et al.   ANALYTICAL CHEMISTRY

Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

(2009) Steve Schoonooghe et al.   BMC BIOTECHNOLOGY

Protein O-mannosylation: Conserved from bacteria to humans

(2009) M. Lommel et al.   GLYCOBIOLOGY

Impact of methionine oxidation on the binding of human IgG1 to FcRn and Fcγ receptors

(2009) Andrea Bertolotti-Ciarlet et al.   MOLECULAR IMMUNOLOGY

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Methionine oxidation in human IgG2 Fc decreases binding affinities to protein A and FcRn

(2009) Hai Pan et al.   PROTEIN SCIENCE

IgG glycosylation analysis

(2009) Carolin Huhn et al.   PROTEOMICS

Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris

(2008) Hyun-Joo Chang et al.   BIOTECHNOLOGY LETTERS

Applications of CE SDS gel in development of biopharmaceutical antibody-based products

(2008) Richard R. Rustandi et al.   ELECTROPHORESIS

Production of monoclonal antibodies by glycoengineered Pichia pastoris

(2008) Thomas I. Potgieter et al.   JOURNAL OF BIOTECHNOLOGY

Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast

(2008) Terrance A Stadheim et al.   Nature Protocols

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008) Christine H. Chung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Enhancing the Production of Fc Fusion Protein in Fed-Batch Fermentation of Pichia pastoris by Design of Experiments

(2007) Henry Lin et al.   BIOTECHNOLOGY PROGRESS

Contribution of Variable Domains to the Stability of Humanized IgG1 Monoclonal Antibodies

(2007) Roxana M. Ionescu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Structural effect of deglycosylation and methionine oxidation on a recombinant monoclonal antibody

(2007) Hongcheng Liu et al.   MOLECULAR IMMUNOLOGY

Glycosylation of Recombinant Antibody Therapeutics

(2005) Royston Jefferis   BIOTECHNOLOGY PROGRESS