Article
Chemistry, Multidisciplinary
David S. Spencer, Aaliyah B. Shodeinde, David W. Beckman, Bryan C. Luu, Hannah R. Hodges, Nicholas A. Peppas
Summary: By evaluating a series of nanoparticle formulations, this study identified candidates with low cytotoxicity, pH-dependent membrane disruption potential, highest siRNA loading, and transfection efficacy for in vitro applications.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Ye Cao, Yang Fei Tan, Yee Shan Wong, Muhammad Aminuddin, Bhuthalingam Ramya, Melvin Wen Jie Liew, Jiaxin Liu, Subbu S. Venkatraman
Summary: Despite the challenges, this study presents a new nanogel system that can encapsulate and sustainably release siRNA, enhancing its efficiency and prolonging the gene silencing effect. This system shows promise for both fundamental biological studies and clinical applications in siRNA-based therapies.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
J. Gutierrez-Alvarez, J. M. Honrubia, A. Sanz-Bravo, E. Gonzalez-Miranda, R. Fernandez-Delgado, M. T. Rejas, S. Zuniga, I Sola, L. Enjuanes
Summary: Deletion of the envelope (E) gene from the Middle East respiratory syndrome coronavirus (MERS-CoV) generates a replication-competent propagation-defective RNA replicon. Further combination with accessory open reading frames (ORFs) can enhance the attenuation of the replicon, resulting in highly safe and promising vaccine candidates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Pharmacology & Pharmacy
Dima Hattab, Amirah Mohd Gazzali, Athirah Bakhtiar
Summary: RNA interference (RNAi) offers a genetic approach for cancer treatment by inhibiting specific messenger RNA signaling uncontrollable cell growth and proliferation. Challenges such as low tumor localization, stability and rapid clearance from circulation hinder the success of gene therapy applications.
Article
Pharmacology & Pharmacy
Patrick Connerty, Ernest Moles, Charles E. de Bock, Nisitha Jayatilleke, Jenny L. Smith, Soheil Meshinchi, Chelsea Mayoh, Maria Kavallaris, Richard B. Lock
Summary: The study developed a new lipid nanoparticle loaded with siRNA for silencing oncogenic lncRNA LINC01257, demonstrating its potential feasibility in the treatment of AML.
Article
Chemistry, Multidisciplinary
Jong Won Lee, Jiwon Choi, Yeonho Choi, Kwangmeyung Kim, Yoosoo Yang, Sun Hwa Kim, Hong Yeol Yoon, Ick Chan Kwon
Summary: RNA interference (RNAi) is a cellular mechanism that regulates gene expression, with small interfering RNA molecules (siRNA) mediating mRNA degradation. Despite the promising therapeutic potential of RNAi technology for diseases like cancer, challenges such as biological instability and limited delivery efficiency remain. Recent advancements in siRNA delivery strategies using cationic lipids and polymers have shown improved pharmacokinetics and delivery efficiency.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Matthew K. Vasher, Gokay Yamankurt, Chad A. Mirkin
Summary: A new hairpin-like siRNA-SNA design is introduced in this study to prevent guide strand dissociation, resulting in enhanced stability, reduced cytotoxicity, and more durable gene knockdown compared to traditional hybridized siRNA-SNAs. This novel approach significantly improves the biological function and establishes a next-generation SNA construct for life science and medical research.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Saba Abbasi Dezfouli, Amarnath P. Rajendran, Jillian Claerhout, Hasan Uludag
Summary: This study aims to engineer novel siRNA/lipopolymer nanocomplexes with anionic additives for gene therapy in breast cancer. The results demonstrate that efficient gene silencing can be achieved with minimal cytotoxicity by optimizing the formulation.
Article
Biochemistry & Molecular Biology
Beinuo Wang, Ruijie Zhang, Yao Wang, Hang Qian, Di Wu, Binfeng He, Hu Liao
Summary: Overcoming cisplatin-based drug resistance in lung cancer remains a challenge globally. Recent studies have shown that Rab GTPases play a role in multiple aspects of tumor progression, including drug resistance. In this study, a nanosystem was developed using Rab26 siRNA-loaded nanoparticles, which effectively transfected cisplatin-resistant lung cancer cells and induced apoptosis while inhibiting autophagy disruption. The combination therapy of siRab26 knockdown with cisplatin improved antitumor therapy in vitro and in vivo. These findings suggest that siRNP is an effective platform for lung cancer therapy in cases of drug resistance.
Article
Engineering, Biomedical
Shrusti S. Patel, Ella N. Hoogenboezem, Fang Yu, Carlisle R. DeJulius, R. Brock Fletcher, Alex G. Sorets, Fiona K. Cherry, Justin H. Lo, Mariah G. Bezold, Nora Francini, Richard d'Arcy, Jordan E. Brasuell, Rebecca S. Cook, Craig L. Duvall
Summary: This study explores the use of ternary siRNA nanoparticles (si-NPs) for gene silencing in tumors. By optimizing the molecular weight and ratio of the core and surface polymer components, safe and effective si-NPs with strong inhibitory effects were developed. This work provides new insights into the structure-function relationships of ternary si-NPs and identifies strategies for safe and potent oncogene silencing.
Article
Pharmacology & Pharmacy
Muhammad Imran Sajid, Dindyal Mandal, Naglaa Salem El-Sayed, Sandeep Lohan, Jonathan Moreno, Rakesh Kumar Tiwari
Summary: Recent approvals of siRNA-based products have led to exploration of siRNA as a potential treatment option for cancer and other diseases. This study introduces a series of oleyl-conjugated histidine-arginine peptides as a promising nonviral siRNA delivery tool.
Article
Multidisciplinary Sciences
Natacha S. Ogando, Priscila El Kazzi, Jessika C. Zevenhoven-Dobbe, Brenda W. Bontes, Alice Decombe, Clara C. Posthuma, Volker Thiel, Bruno Canard, Francois Ferron, Etienne Decroly, Eric J. Snijder
Summary: The study found that N7-MTase is a critical enzyme for betacoronavirus replication and essential for virus viability. By conducting mutagenesis experiments on betacoronavirus nonstructural protein 14, key residues of the catalytic pocket were identified, which could be targeted for designing inhibitors with potential pan-coronaviral activity spectrum.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Zhiqing Zhang, Dongyan Xu, Jiawei Wang, Ruyan Zhang, Huan Du, Ting Zhou, Xiufeng Wang, Fang Wang
Summary: This study designed a DNA nano-assembly based on rolling circle amplification that can co-deliver doxorubicin (Dox) and siRNA simultaneously, achieving a comprehensive effect in tumor treatment.
Review
Chemistry, Multidisciplinary
Bijan Emiliano Ferdows, Dylan Neal Patel, Wei Chen, Xiangang Huang, Na Kong, Wei Tao
Summary: This article discusses the research progress in using nanoparticles to deliver therapeutic RNAs for cancer therapy. By selectively regulating cancer-specific genes, researchers have successfully suppressed tumor progression and metastasis, overcoming the degradation of exogenous RNAs by the human immune system. Various types of nanoparticles have been developed for delivering therapeutic RNAs and eliciting tumor suppressing responses.
Article
Chemistry, Multidisciplinary
Haohao Yin, Liping Sun, Yinying Pu, Jifeng Yu, Wei Feng, Caihong Dong, Bangguo Zhou, Dou Du, Yan Zhang, Yu Chen, Huixiong Xu
Summary: This study identified the activation of NFE2L2 during SDT, which hindered the treatment efficacy. A novel HMME @ Lip-Cas9 system was designed to enhance therapeutic efficiency, laying the groundwork for new approaches in HCC management.
ACS CENTRAL SCIENCE
(2021)
Letter
Public, Environmental & Occupational Health
Faisal Ismail, Atiya Farag, Soghra Haq, Mohammad Amjad Kamal
DISASTER MEDICINE AND PUBLIC HEALTH PREPAREDNESS
(2023)
Review
Endocrinology & Metabolism
Nobendu Mukerjee, Swastika Maitra, Subhradeep Roy, Shaswata Modak, Mohammad Mehedi Hasan, Biswajit Chakraborty, Arabinda Ghosh, Asmita Ghosh, Mohammad Amjad Kamal, Abhijit Dey, Ghulam Md Ashraf, Sumira Malik, Md Habibur Rahman, Badrah S. Alghamdi, Adel Mohammad Abuzenadah, Athanasios Alexiou
Summary: Glioblastoma is an aggressive tumor that hinders normal brain function, and the inability of the skull to expand results in increased pressure in the brain. Recent studies have found that SNPs play a significant role in the development and spread of glioblastoma, with many GB-related SNPs being highly abundant in the promoter region, creating genetic vulnerability to glioma.
METABOLIC BRAIN DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Etimad Huwait, Rehab Almassabi, Sanaa Almowallad, Salma Saddeek, Sajjad Karim, Gauthaman Kalamegam, Zeenat Mirza
Summary: Myricetin, a flavonol found in certain foods, has been found to have anti-inflammatory properties and can prevent the occurrence of macrophage infiltration, lipid deposition, and arterial wall thickening, which are characteristic of atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Shailja Singhal, Utsang Kumar, Taha Alqahtani, Igor Vladimirovich Rzhepakovsky, Rekha Khandia, Megha Pandey, Saud Alqahtani, Hanan Alharbi, Mohammad Amjad Kamal
Summary: Apoptosis and autophagy are two fundamental processes involved in maintaining homeostasis, and they can be initiated by a common stimulus. Autophagy has been implicated in various diseases, including viral infections. Genetic manipulations that alter gene expression could be a strategy to control viral infection.
CURRENT PHARMACEUTICAL DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Mandeep Chouhan, Prashant Kumar Tiwari, Mahmoud Moustafa, Kundan Kumar Chaubey, Arti Gupta, Rajeev Kumar, Amaresh Kumar Sahoo, Esam I. Azhar, Vivek Dhar Dwivedi, Sanjay Kumar
Summary: Approximately 25% of the global population carries latent Mycobacterium tuberculosis (Mtb) infection, with only 5-10% progressing to active tuberculosis (TB) and the remaining 90-95% remaining in a latent state. The resuscitation-promoting factor B (RpfB) has been identified as a promising target for tuberculosis drug discovery due to its role in reactivating latent TB infection. This study used computational methods to identify potential natural compounds that inhibit the Mtb RpfB protein, providing a cost-effective approach for further in vitro and in vivo validation experiments.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Sayed Sartaj Sohrab, Riya Raj, Amka Nagar, Susan Hawthorne, Ana Claudia Paiva-Santos, Mohammad Amjad Kamal, Mai M. M. El-Daly, Esam I. I. Azhar, Ankur Sharma
Summary: The body's immune response to an invading pathogen that causes infection results in inflammation, which can lead to chronic inflammatory diseases and cancer. Inflammation can be short-term and non-specific, or long-term and specific, causing angiogenesis, fibrosis, tissue destruction, and cancer progression.
Article
Oncology
Zeenat Mirza, Md Shahid Ansari, Md Shahid Iqbal, Nesar Ahmad, Nofe Alganmi, Haneen Banjar, Mohammed H. H. Al-Qahtani, Sajjad Karim
Summary: Using AI and machine learning, a diagnostic model and a prognostic model for breast cancer classification were constructed based on transcriptomics data. Through experimental validation, a nine-gene signature for diagnosis and an eight-gene signature for prognosis were identified, showing potential in improving the accuracy and cost-effectiveness of breast cancer management.
Article
Multidisciplinary Sciences
Sayed Sartaj Sohrab, Sherif Aly El-Kafrawy, Zeenat Mirza, Ahmed M. Hassan, Fatima Alsaqaf, Esam Ibraheem Azhar
Summary: A new coronavirus, MERS-CoV, was discovered in Jeddah, Saudi Arabia in 2012. It has been reported in 27 countries and can be transmitted to humans from camels. There is currently no vaccine or antiviral therapy available for this virus.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Pediatrics
Sajjad Karim, Ibtessam Ramzi Hussein, Hans-Juergen Schulten, Saad Alsaedi, Zeenat Mirza, Mohammed Al-Qahtani, Adeel Chaudhary
Summary: This study aimed to investigate the genetic heterogeneity in Saudi children with developmental delay (DD), congenital malformations (CM), and intellectual disability (ID). High-resolution array comparative genomic hybridization (array CGH) was used to detect disease-associated copy number variations (CNVs). The diagnosis rate of array CGH was around two-fold higher than that of standard karyotyping.
Article
Biochemistry & Molecular Biology
Divyanshi Shukla, Amer M. Alanazi, Siva Prasad Panda, Vivek Dhar Dwivedi, Mohammad Amjad Kamal
Summary: In this study, a QSAR-based chemical library screening was conducted to identify potential RdRp inhibitors from the Meliaceae family. The top two hit compounds were further analyzed using molecular docking and molecular dynamics simulations, which showed stable binding interactions with RdRp and comparable binding energies to known inhibitors. Compound IMPHY009135 exhibited the strongest binding affinity, while compound IMPHY009276 showed the most stable structure. These findings suggest that these two compounds could be potential lead compounds for developing novel antiviral agents against ZIKV.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Leena H. Bajrai, Thamir A. Alandijany, Isra Alsaady, Mai M. El-Daly, Ahmed M. Tolah, Aiah M. Khateb, Amit Dubey, Vivek Dhar Dwivedi, Esam I. Azhar
Summary: This study used molecular dynamics modeling and high-throughput virtual screening to evaluate the antiviral activity of anti-dengue drugs against Japanese encephalitis virus Non-structural 5 polymerase. MM/GBSA analysis was performed to rank the most promising compounds. Molecular dynamics simulation confirmed the stable binding of the three compounds, and MM/GBSA analysis further confirmed the high binding affinity of two compounds. Therefore, these three anti-dengue molecules have the potential to be used as a remedy for Japanese encephalitis.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biology
Gnana Deepthi Medarametla, Ripudaman Singh Kahlon, Lampimukhi Mahitha, Sanobar Shariff, Naga Praneeth Vakkalagadda, Hitesh Chopra, Mohammad Amjad Kamal, Neil Patel, Yashendra Sethi, Nirja Kaka
Summary: Amyloidosis is a protein deposition disorder that damages organ function by causing the accumulation of insoluble fibrils in bodily tissues. Cardiac amyloidosis, a severe and under-reported condition, involves the accumulation of amyloid in the extracellular area of the myocardium, leading to thickening and stiffening of ventricular walls. The incidence of cardiac amyloidosis has been slowly increasing, and this study aims to provide a comprehensive understanding of its pathophysiology and clinical presentation, as well as offer up-to-date approaches for diagnosis and therapy. Genetic mutations and advancements in imaging and diagnostic methods contribute to the growing incidence of cardiac amyloidosis. Early detection can help improve treatment outcomes, and various therapeutic approaches are being explored to impede disease progression and enhance clinical outcomes.
Review
Biochemistry & Molecular Biology
Ligen Yu, Mohd Khanapi Abd Ghani, Alessio Aghemo, Debmalya Barh, Matteo Bassetti, Fausto Catena, Gaetano Gallo, Ali Gholamrezanezhad, Mohammad Amjad Kamal, Amos Lal, Kamal Kant Sahu, Shailendra K. Saxena, Ugo Elmore, Farid Rahimi, Chiara Robba, Yuanlin Song, Zhengyuan Xia, Boxuan Yu
Summary: This study analyzed keywords and found that factors such as the immune system and nutrition are related to COVID-19. It proposed that the human immune system is a complex system that employs self-destruct and rebuild strategies to fight microbial infections. Microbial infections may be self-limiting and provide essential nutrition for the patient. The study also discussed possible strategies to mitigate COVID-19, including vaccination.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Computer Science, Artificial Intelligence
Pongtep Weerapong, Kotaro Hashikura, Md Abdus Samad Kamal, Iwanori Murakami, Kou Yamada
Summary: This paper discusses a method to evaluate the prediction accuracy of a wheelchair-occupant simulation model by comparing it with real-world experimental results. Different types of seat cushions are used in the model to simulate the low-frequency vibration waves caused by the interaction between wheelchair tires and the ground. The analysis shows that the occupant's torso is most affected by the vibration, resulting in the maximum displacement of organs; and the foam-based cushion performs the best in reducing the amplitude of displacement responses.
INTERNATIONAL JOURNAL OF INNOVATIVE COMPUTING INFORMATION AND CONTROL
(2023)
Article
Computer Science, Artificial Intelligence
Sorawin Phukapak, Daisuke Koyama, Kotaro Hashikura, Md Abdus Samad Kamal, Kou Yamada
Summary: In this paper, the parameterization of all disturbance observers for periodic output disturbances is examined. Disturbance observers have been used to estimate disturbances in plants. While previous papers have clarified the parameterization of all disturbance observers for plants, none have focused on periodic output disturbances. This paper proposes a parameterization method for all disturbance observers for periodic output disturbances.
INTERNATIONAL JOURNAL OF INNOVATIVE COMPUTING INFORMATION AND CONTROL
(2023)
