Structure–function analysis of the nsp14 N7–guanine methyltransferase reveals an essential role in Betacoronavirus replication

New targets for drug design: Importance of nsp14/nsp10 complex formation for the 3’‐5’ exoribonucleolytic activity on SARS‐CoV‐2

(2021) Margarida Saramago et al.   FEBS Journal

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferase

(2021) Souradeep Basu et al.   BIOCHEMICAL JOURNAL

Coupling of N7-methyltransferase and 3′-5′ exoribonuclease with SARS-CoV-2 polymerase reveals mechanisms for capping and proofreading

(2021) Liming Yan et al.   CELL

Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme

(2021) Chang Liu et al.   SCIENCE

A pneumonia outbreak associated with a new coronavirus of probable bat origin

(2020) Peng Zhou et al.   NATURE

The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2

(2020)   Nature Microbiology

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

(2020) Tran Thi Nhu Thao et al.   NATURE

A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis

(2020) Eric J. Snijder et al.   PLOS BIOLOGY

Synthesis of adenine dinucleosides SAM analogs as specific inhibitors of SARS-CoV nsp14 RNA cap guanine-N7-methyltransferase

(2020) Rostom Ahmed-Belkacem et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

The enzymatic activity of the nsp14 exoribonuclease is critical for replication of MERS-CoV and SARS-CoV-2

(2020) Natacha S. Ogando et al.   JOURNAL OF VIROLOGY

Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

(2020) Liming Yan et al.   CELL

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

(2020) Steffen Klein et al.   Nature Communications

The Curious Case of the Nidovirus Exoribonuclease: Its Role in RNA Synthesis and Replication Fidelity

(2019) Natacha S. Ogando et al.   Frontiers in Microbiology

Functional and evolutionary analysis of viral proteins containing a Rossmann-like fold

(2018) Kirill E. Medvedev et al.   PROTEIN SCIENCE

Rossmann-Fold Methyltransferases: Taking a “β-Turn” around Their Cofactor, S-Adenosylmethionine

(2018) Bhanu Pratap Singh Chouhan et al.   BIOCHEMISTRY

Origin and evolution of pathogenic coronaviruses

(2018) Jie Cui et al.   NATURE REVIEWS MICROBIOLOGY

A planarian nidovirus expands the limits of RNA genome size

(2018) Amir Saberi et al.   PLoS Pathogens

Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

(2017) François Ferron et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improvements to the APBS biomolecular solvation software suite

(2017) Elizabeth Jurrus et al.   PROTEIN SCIENCE

Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus

(2017) Ben Hu et al.   PLoS Pathogens

Mutagenesis ofS-Adenosyl-l-Methionine-Binding Residues in Coronavirus nsp14 N7-Methyltransferase Demonstrates Differing Requirements for Genome Translation and Resistance to Innate Immunity

(2016) James Brett Case et al.   JOURNAL OF VIROLOGY

mRNA capping: biological functions and applications

(2016) Anand Ramanathan et al.   NUCLEIC ACIDS RESEARCH

Middle East Respiratory Coronavirus Accessory Protein 4a Inhibits PKR-Mediated Antiviral Stress Responses

(2016) Huib H. Rabouw et al.   PLoS Pathogens

Better prediction of functional effects for sequence variants

(2015) Maximilian Hecht et al.   BMC GENOMICS

Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived PeptideIn VitroandIn VivoTo Reduce Replication and Pathogenesis

(2015) Yi Wang et al.   JOURNAL OF VIROLOGY

A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence

(2015) Vineet D Menachery et al.   NATURE MEDICINE

Discovery of an essential nucleotidylating activity associated with a newly delineated conserved domain in the RNA polymerase-containing protein of all nidoviruses

(2015) Kathleen C. Lehmann et al.   NUCLEIC ACIDS RESEARCH

Structural basis and functional analysis of the SARS coronavirus nsp14–nsp10 complex

(2015) Yuanyuan Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA methyltransferases involved in 5′ cap biosynthesis

(2015) Magdalena Byszewska et al.   RNA Biology

Yeast-based assays for the high-throughput screening of inhibitors of coronavirus RNA cap guanine-N7-methyltransferase

(2014) Ying Sun et al.   ANTIVIRAL RESEARCH

To sense or not to sense viral RNA—essentials of coronavirus innate immune evasion

(2014) Eveline Kindler et al.   CURRENT OPINION IN MICROBIOLOGY

CoronavirusNsp10, a Critical Co-factor for Activation of Multiple Replicative Enzymes

(2014) Mickaël Bouvet et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The nsp3 Macrodomain Promotes Virulence in Mice with Coronavirus-Induced Encephalitis

(2014) Anthony R. Fehr et al.   JOURNAL OF VIROLOGY

MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-  treatment

(2013) A. H. de Wilde et al.   JOURNAL OF GENERAL VIROLOGY

Structure-Function Analysis of Severe Acute Respiratory Syndrome Coronavirus RNA Cap Guanine-N7-Methyltransferase

(2013) Y. Chen et al.   JOURNAL OF VIROLOGY

Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor

(2013) Xing-Yi Ge et al.   NATURE

Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP

(2013) Xu Jin et al.   VIRUS RESEARCH

Engineering a Replication-Competent, Propagation-Defective Middle East Respiratory Syndrome Coronavirus as a Vaccine Candidate

(2013) F. Almazan et al.   mBio

Structural and functional studies of S-adenosyl-L-methionine binding proteins: a ligand-centric approach

(2013) Rajaram Gana et al.   BMC STRUCTURAL BIOLOGY

Coronaviruses Lacking Exoribonuclease Activity Are Susceptible to Lethal Mutagenesis: Evidence for Proofreading and Potential Therapeutics

(2013) Everett Clinton Smith et al.   PLoS Pathogens

The viral RNA capping machinery as a target for antiviral drugs

(2012) François Ferron et al.   ANTIVIRAL RESEARCH

Reverse Genetics of SARS-Related Coronavirus Using Vaccinia Virus-Based Recombination

(2012) Sjoerd H. E. van den Worm et al.   PLoS One

RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex

(2012) M. Bouvet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality

(2011) Yves Dehouck et al.   BMC BIOINFORMATICS

Conventional and unconventional mechanisms for capping viral mRNA

(2011) Etienne Decroly et al.   NATURE REVIEWS MICROBIOLOGY

Coronaviruses

(2011) Mark R. Denison et al.   RNA Biology

Discovery of the First Insect Nidovirus, a Missing Evolutionary Link in the Emergence of the Largest RNA Virus Genomes

(2011) Phan Thi Nga et al.   PLoS Pathogens

Biochemical and Structural Insights into the Mechanisms of SARS Coronavirus RNA Ribose 2′-O-Methylation by nsp16/nsp10 Protein Complex

(2011) Yu Chen et al.   PLoS Pathogens

Viruses know more than one way to don a cap

(2010) Eugene V. Koonin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Arterivirus Nsp1 Modulates the Accumulation of Minus-Strand Templates to Control the Relative Abundance of Viral mRNAs

(2010) Danny D. Nedialkova et al.   PLoS Pathogens

In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation

(2010) Mickaël Bouvet et al.   PLoS Pathogens

Enzymology of RNA cap synthesis

(2010) Agnidipta Ghosh et al.   Wiley Interdisciplinary Reviews-RNA

SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building

(2009) M. Gouy et al.   MOLECULAR BIOLOGY AND EVOLUTION

Functional screen reveals SARS coronavirus nonstructural protein nsp14 as a novel cap N7 methyltransferase

(2009) Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

(2009) Susanne Pfefferle et al.   Virology Journal

Coronavirus Nonstructural Protein 16 Is a Cap-0 Binding Enzyme Possessing (Nucleoside-2'O)-Methyltransferase Activity

(2008) E. Decroly et al.   JOURNAL OF VIROLOGY

Detecting evolutionary relationships across existing fold space, using sequence order-independent profile-profile alignments

(2008) L. Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SARS-Coronavirus Replication Is Supported by a Reticulovesicular Network of Modified Endoplasmic Reticulum

(2008) Kèvin Knoops et al.   PLOS BIOLOGY

SARS-Coronavirus Replication/Transcription Complexes Are Membrane-Protected and Need a Host Factor for Activity In Vitro

(2008) Martijn J. van Hemert et al.   PLoS Pathogens