Article
Plant Sciences
Gernot Beihammer, Julia Koenig-Beihammer, Benjamin Kogelmann, Valentina Ruocco, Clemens Gruenwald-Gruber, Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Pooja Saxena, Johannes S. S. Gach, Herta Steinkellner, Richard Strasser
Summary: By testing a single-subunit oligosaccharyltransferase from Leishmania donovani (LdOST), we found that it can significantly increase the Fc glycosylation level of IgG1 without changing the overall N-glycan composition. This finding is important for reducing the heterogeneity of plant-produced antibodies and improving their stability and effector functions.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Tobias Strittmatter, Sabina Egli, Adrian Bertschi, Richard Plieninger, Daniel Bojar, Mingqi Xie, Martin Fussenegger
Summary: In this study, a gene switch using l-glucose and d-idonate to regulate gene expression was designed and successfully utilized to control the production of rituximab.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Plant Sciences
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Summary: N-glycan engineering allows for the optimization and quality control of recombinant antibodies. Nicotiana benthamiana has emerged as an attractive production system for therapeutic antibodies. In this study, a CD27-targeting monoclonal antibody Varlilumab was successfully produced in N. benthamiana with biantennary β1,4-galactosylated N-glycan structures through N-glycan engineering.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Yongjing Xie, Leticia Martins Mota, Adam Bergin, Roisin O'Flaherty, Aled Jones, Bethan Morgan, Michael Butler
Summary: This study presents an improved 96-well plate format platform for streamlined glycan profiling, allowing high sensitivity and high-throughput identification and quantification of diverse N-glycans. The technology offers a powerful tool for rapid advancement in glycan analysis for biopharmaceutical development and biomarker discovery in clinical disease diagnosis.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
David Fiebig, Jan P. Bogen, Stefania C. Carrara, Lukas Deweid, Stefan Zielonka, Julius Grzeschik, Bjoern Hock, Harald Kolmar
Summary: A new approach was presented to facilitate the transition from antibody screening using YSD Fab libraries to the production of full-length IgG antibodies. By utilizing Golden Gate Cloning and a bidirectional promoter system, a novel, straightforward, and time-saving workflow was established. This streamlining of antibody hit discovery efforts may lead to the faster characterization of antibodies against various targets for the development of new therapeutic agents.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kara M. Gillmann, J. Sebastian Temme, Samantha Marglous, Claire E. Brown, Jeffrey C. Gildersleeve
Summary: Anti-glycan monoclonal antibodies (mAbs) have diverse applications in human health and basic research, including cancer diagnostics and therapeutics. Despite the limited supply of high-quality anti-glycan mAbs, recent advances have been made in targeting cancer- and infectious disease-associated glycans, providing new opportunities for discovery and development of therapeutic antibodies.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ines F. Pinto, Ruben R. G. Soares, Meeri E-L Makinen, Veronique Chotteau, Aman Russom
Summary: The rapidly growing biopharmaceutical market emphasizes the importance of balancing clinical safety with profitability through critical R&D efforts. New technologies highlight the complexity of cell culture processes, while the lack of simple, rapid, and cost-effective devices for routine protein monitoring is a current challenge.
Article
Cell Biology
Charles D. Murin, Pavlo Gilchuk, Philipp A. Ilinykh, Kai Huang, Natalia Kuzmina, Xiaoli Shen, Jessica F. Bruhn, Aubrey L. Bryan, Edgar Davidson, Benjamin J. Doranz, Lauren E. Williamson, Jeffrey Copps, Tanwee Alkutkar, Andrew Flyak, Alexander Bukreyev, James E. Crowe, Andrew B. Ward
Summary: This study identified antibodies targeting the glycan cap epitope on the ebolavirus glycoprotein with broad neutralizing activity, and revealed the structural features of these antibodies working in synergy with base-binding antibodies. The study provides a molecular basis for ebolavirus neutralization by broadly reactive glycan cap antibodies.
Article
Biotechnology & Applied Microbiology
Yen-Hsi Chen, Weihua Tian, Makiko Yasuda, Zilu Ye, Ming Song, Ulla Mandel, Claus Kristensen, Lorenzo Povolo, Andre R. A. Marques, Tomislav Caval, Albert J. R. Heck, Julio Lopes Sampaio, Ludger Johannes, Takahiro Tsukimura, Robert Desnick, Sergey Y. Y. Vakhrushev, Zhang Yang, Henrik Clausen
Summary: Currently available enzyme replacement therapies for lysosomal storage diseases are limited in their effectiveness due to short circulation times and suboptimal biodistribution of the therapeutic enzymes. Researchers have engineered Chinese hamster ovary (CHO) cells to produce glycoengineered enzymes, which have improved circulation time and biodistribution. This glycoengineering approach, known as Long-Acting-GlycoDesign (LAGD), may be widely applicable to lysosomal replacement enzymes to improve their circulatory stability and therapeutic efficacy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biology
Taiki Kuribara, Kiichiro Totani
Summary: Glycans have diverse roles in biological processes, including mediating cell-cell interaction, viral infection, and glycoprotein folding in the endoplasmic reticulum (ER). This review summarizes the relationship between oligomannose-type glycan processing in the ER and misfolding diseases caused by disruption of ER homeostasis. The review also discusses the potential of alteration in oligomannose-type glycan processing as indicators of ER stress-related diseases.
Article
Multidisciplinary Sciences
Meiyan Jin, Cyna Shirazinejad, Bowen Wang, Amy Yan, Johannes Schoneberg, Srigokul Upadhyayula, Ke Xu, David G. Drubin
Summary: This study investigates the organization of actin networks at clathrin-mediated endocytosis (CME) sites and how actin assembly is regulated. The findings show that actin assembles preferentially at stalled CME sites, where it exerts an asymmetric force to pull vesicles into the cell. N-WASP and the Arp2/3 complex are recruited to one side of CME sites for stimulating asymmetric actin assembly.
NATURE COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Natalie Hammond, Jamie Snider, Igor Stagljar, Kevin Mitchell, Kirill Lagutin, Matthew Jessulat, Mohan Babu, Paul H. Teesdale-Spittle, Jeffrey P. Sheridan, Stephen L. Sturley, Andrew B. Munkacsi
Summary: A study identified 11 proteins that interact with the yeast Ncr1 protein, mainly located in the endoplasmic reticulum membrane and influenced by sterol metabolism. The lack of Ncr1-Cyb5 interaction leads to the accumulation of cholesterol and sphingolipids, resembling the symptoms of NP-C disease.
Review
Immunology
Meihui Xu, Chi Ma, Ming Dong, Chunjie Guo, Simin Yang, Yue Liu, Xu Wang
Summary: This study aims to characterize the clinical symptoms, MRI findings, and prognosis of adult cerebral cortical encephalitis (CCE) with anti-MOG antibody. Two adult cases of CCE with anti-MOG antibody were presented and a systematic review of the literature was conducted. The majority of patients had a favorable prognosis after treatment, highlighting the importance of early detection and immunotherapy in improving outcomes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Ronald Derking, Joel D. Allen, Christopher A. Cottrell, Kwinten Sliepen, Gemma E. Seabright, Wen-Hsin Lee, Yoann Aldon, Kimmo Rantalainen, Aleksandar Antanasijevic, Jeffrey Copps, Anila Yasmeen, Albert Cupo, Victor M. Cruz Portillo, Meliawati Poniman, Niki Bol, Patricia van Der Woude, Steven W. de Taeye, Tom L. G. M. van den Kerkhof, P. J. Klasse, Gabriel Ozorowski, Marit J. van Gils, John P. Moore, Andrew B. Ward, Max Crispin, Rogier W. Sanders
Summary: Artificial glycan holes can induce strain-specific neutralizing antibodies or non-neutralizing antibodies on recombinant Env-based vaccines. By replacing NxS with NxT, researchers have successfully increased the occupancy of most N-linked glycosylation sites to mimic native glycosylation and eliminate glycan holes.
Article
Chemistry, Multidisciplinary
Kota Nomura, Ryo Okamoto, Yuta Maki, Ayumu Hayashibara, Toshifumi Takao, Tomoya Fukuoka, Eiji Miyoshi, Bradley L. Pentelute, Yasuhiro Kajihara
Summary: SPINK13 is a secreted protein that has been studied as a therapeutic drug and biomarker for cancer cells. The existence and functions of N-glycosylation in SPINK13 are still unclear. This study successfully synthesized the scarce N-glycosylated form of SPINK13 using a chemical synthesis method, providing a new approach for the preparation of glycosylated SPINK13.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Analytical
Yoshio Suzuki, Atsushi Kuno, Yasunori Chiba
SENSORS AND ACTUATORS B-CHEMICAL
(2015)
Article
Biochemistry & Molecular Biology
Fumie Saito, Izumi Sakamoto, Akio Kanatani, Yasunori Chiba
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2016)
Article
Biochemistry & Molecular Biology
Hiroko Abe, Kazuya Tomimoto, Yasuko Fujita, Tomoko Iwaki, Yasunori Chiba, Ken-ichi Nakayama, Yoshihiro Nakajima
Article
Biochemistry & Molecular Biology
Takashi Sato, Hiroaki Tateno, Hiroyuki Kaji, Yasunori Chiba, Tomomi Kubota, Jun Hirabayashi, Hisashi Narimatsu
Article
Biotechnology & Applied Microbiology
Takeshi Suzuki, Satoshi Baba, Minako Ono, Koichi Nonaka, Kimihisa Ichikawa, Masayuki Yabuta, Rie Ito, Yasunori Chiba
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2017)
Article
Biochemistry & Molecular Biology
Xin-Xin Xu, Akiko Komatsuzaki, Yasunori Chiba, Xiao-Dong Gao, Takehiko Yoko-o
Article
Biochemistry & Molecular Biology
Ze-Cheng Jin, Toshihiko Kitajima, Weijie Dong, Yi-Fan Huang, Wei-Wei Ren, Feng Guan, Yasunori Chiba, Xiao-Dong Gao, Morihisa Fujita
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Toshihiko Kitajima, Yuanling Jia, Akiko Komatsuzaki, Juan Cui, Fumiko Matsuzawa, Sei-Ichi Aikawa, Xiao-Dong Gao, Yasunori Chiba
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2018)
Article
Biotechnology & Applied Microbiology
Takehiko Yoko-o, Akiko Komatsuzaki, Erina Yoshihara, Mariko Umemura, Yasunori Chibal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biochemical Research Methods
Hiroyuki Kaji, Makoto Ocho, Akira Togayachi, Atsushi Kuno, Maki Sogabe, Takashi Ohkura, Hirofumi Nozaki, Takashi Angata, Yasunori Chiba, Hidenori Ozaki, Jun Hirabayashi, Yasuhito Tanaka, Masashi Mizokami, Yuzuru Ikehara, Hisashi Narimatsu
JOURNAL OF PROTEOME RESEARCH
(2013)
Article
Endocrinology & Metabolism
Tadayasu Togawa, Masaru Takada, Yoshiaki Aizawa, Takahiro Tsukimura, Yasunori Chiba, Hitoshi Sakuraba
MOLECULAR GENETICS AND METABOLISM
(2014)
Article
Multidisciplinary Sciences
Yayoi Yoshimura, Kaori Denda-Nagai, Yoshie Takahashi, Izuru Nagashima, Hiroki Shimizu, Toshimitsu Kishimoto, Miki Noji, Shigeyuki Shichino, Yasunori Chiba, Tatsuro Irimura
SCIENTIFIC REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Takehiko Yoko-o, Akiko Komatsuzaki, Erina Yoshihara, Song Zhao, Mariko Umemura, Xiao-Dong Gao, Yasunori Chiba
Summary: In this study, the regulation mechanisms of the AOX1 promoter in O. minuta were investigated using three putative transcription factor genes TRM1, TRM2, and MPP1. Results showed that TRM1 and MPP1 regulate the transcription of AOX1 in the same pathway, while TRM2 regulates it in another pathway. Additionally, it was found that these two pathways compensate for each other and have crosstalk mechanisms with each other.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Masashi Tsuda, Yuki Nakatani, Satoshi Baba, Isshin Tanaka, Kimihisa Ichikawa, Koichi Nonaka, Rie Ito, Takehiko Yoko-o, Yasunori Chiba
Summary: To select the best strain for heterologous protein expression, researchers investigated the ability to produce secretory proteins and various genotypic and phenotypic characteristics. NBRC 10746 showed the highest performance in Cypridina nocti-luca luciferase expression. A promising host for heterologous protein expression, clone #5-30 (tat06213), was obtained from NBRC 10746 through single-colony isolation. Further analysis revealed differences in genetic sequences and chromosome structures among the tested O. minuta var. minuta strains, which might explain the variations in heterologous protein expression.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Yukiko Kamiya, Kotaro Yanagi, Toshihiko Kitajima, Takumi Yamaguchi, Yasunori Chiba, Koichi Kato