Glycoengineering in CHO Cells: Advances in Systems Biology

Fresh from the biotech pipeline—2016

(2017) Chris Morrison   NATURE BIOTECHNOLOGY

Public biotech in 2016—the numbers

(2017) Chris Morrison et al.   NATURE BIOTECHNOLOGY

Model-based analysis of N-glycosylation in Chinese hamster ovary cells

(2017) Frederick J. Krambeck et al.   PLoS One

Glycoproteomics: A Balance between High-Throughput and In-Depth Analysis

(2017) Yang Yang et al.   TRENDS IN BIOTECHNOLOGY

Investigation of O-glycosylation heterogeneity of recombinant coagulation factor IX using LC–MS/MS

(2017) Youngsuk Seo et al.   Bioanalysis

Integrated Genome and Protein Editing Swaps α -2,6 Sialylation for α -2,3 Sialic Acid on Recombinant Antibodies from CHO

(2017) Cheng-yu Chung et al.   Biotechnology Journal

Predictive glycoengineering of biosimilars using a Markov chain glycosylation model

(2016) Philipp N. Spahn et al.   Biotechnology Journal

Chemical Glycoproteomics

(2016) Krishnan K. Palaniappan et al.   CHEMICAL REVIEWS

Glyco-Engineering of Human IgG-Fc to Modulate Biologic Activities

(2016) Roy Jefferis   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

An omics approach to rational feed

(2016) Eric J.M. Blondeel et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines

(2016) Yusuke Mimura et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A Markov chain model for N-linked protein glycosylation – towards a low-parameter tool for model-driven glycoengineering

(2016) Philipp N. Spahn et al.   METABOLIC ENGINEERING

At least two Fc Neu5Gc residues of monoclonal antibodies are required for binding to anti-Neu5Gc antibody

(2016) Chuanfei Yu et al.   Scientific Reports

Predictive glycoengineering of biosimilars using a Markov chain glycosylation model

(2016) Philipp N. Spahn et al.   Biotechnology Journal

Posttranslational Modifications and the Immunogenicity of Biotherapeutics

(2016) Roy Jefferis   Journal of Immunology Research

Immunogenicity of Biotherapeutics: Causes and Association with Posttranslational Modifications

(2016) Anshu Kuriakose et al.   Journal of Immunology Research

Advancements in mass spectrometry-based glycoproteomics and glycomics

(2016) Haojie Lu et al.   National Science Review

A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism

(2016) Hooman Hefzi et al.   Cell Systems

Advances in mass spectrometry driven O-glycoproteomics

(2015) Steven B. Levery et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Multi-omic profiling ­of EPO-producing Chinese hamster ovary cell panel reveals metabolic adaptation to heterologous protein production

(2015) Daniel Ley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation

(2015) Bojiao Yin et al.   BIOTECHNOLOGY AND BIOENGINEERING

The use of ‘Omics technology to rationally improve industrial mammalian cell line performance

(2015) Amanda M. Lewis et al.   BIOTECHNOLOGY AND BIOENGINEERING

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

(2015) Kah Fai Chan et al.   Biotechnology Journal

Effect of Bcl-xLoverexpression on sialylation of Fc-fusion protein in recombinant Chinese hamster ovary cell cultures

(2015) Jong Hyun Lee et al.   BIOTECHNOLOGY PROGRESS

Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

(2015) Ernst Böhm et al.   BMC BIOTECHNOLOGY

O -glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O -glycan core enzyme cDNAs

(2015) Jining Liu et al.   JOURNAL OF BIOTECHNOLOGY

Production of α2,6-sialylated IgG1 in CHO cells

(2015) Céline Raymond et al.   mAbs

Engineered CHO cells for production of diverse, homogeneous glycoproteins

(2015) Zhang Yang et al.   NATURE BIOTECHNOLOGY

A common glycan structure on immunoglobulin G for enhancement of effector functions

(2015) Chin-Wei Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

(2015) Kah Fai Chan et al.   Biotechnology Journal

Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities

(2015) Masaki Kurogochi et al.   PLoS One

Analysis of 3-O-sulfo group-containing heparin tetrasaccharides in heparin by liquid chromatography–mass spectrometry

(2014) Guoyun Li et al.   ANALYTICAL BIOCHEMISTRY

Non-anticoagulant derivatives of heparin for the management of asthma: distant dream or close reality?

(2014) Madhur D Shastri et al.   EXPERT OPINION ON INVESTIGATIONAL DRUGS

Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism

(2014) Anas M Abdel Rahman et al.   GLYCOBIOLOGY

Towards Controlling the Glycoform: A Model Framework Linking Extracellular Metabolites to Antibody Glycosylation

(2014) Philip Jedrzejewski et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Galactosyltransferase 4 is a major control point for glycan branching in N-linked glycosylation

(2014) A. G. McDonald et al.   JOURNAL OF CELL SCIENCE

Application of Multi-Omics Techniques for Bioprocess Design and Optimization in Chinese Hamster Ovary Cells

(2014) Amy Farrell et al.   JOURNAL OF PROTEOME RESEARCH

Versatile characterization of glycosylation modification in CTLA4-Ig fusion proteins by liquid chromatography-mass spectrometry

(2014) Lei Zhu et al.   mAbs

The GalNAc-type O-Glycoproteome of CHO Cells Characterized by the SimpleCell Strategy

(2014) Zhang Yang et al.   MOLECULAR & CELLULAR PROTEOMICS

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Inverse hormesis of cancer growth mediated by narrow ranges of tumor-directed antibodies

(2014) O. M. T. Pearce et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

N- and O-Glycosylation Analysis of Etanercept Using Liquid Chromatography and Quadrupole Time-of-Flight Mass Spectrometry Equipped with Electron-Transfer Dissociation Functionality

(2013) Stephane Houel et al.   ANALYTICAL CHEMISTRY

An 'omics approach towards CHO cell engineering

(2013) Payel Datta et al.   BIOTECHNOLOGY AND BIOENGINEERING

The emerging CHO systems biology era: harnessing the ‘omics revolution for biotechnology

(2013) Helene Faustrup Kildegaard et al.   CURRENT OPINION IN BIOTECHNOLOGY

Heparin and Heparin Binding Proteins: Potential Relevance to Reproductive Physiology

(2013) Vikash Kumar Yadav et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Adjunct low-molecular-weight heparin to improve live birth rate after recurrent implantation failure: a systematic review and meta-analysis

(2013) N. Potdar et al.   HUMAN REPRODUCTION UPDATE

Bioengineered Chinese Hamster Ovary Cells with Golgi-targeted 3-O-Sulfotransferase-1 Biosynthesize Heparan Sulfate with an Antithrombin-binding Site

(2013) Payel Datta et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering Hydrophobic Protein–Carbohydrate Interactions to Fine-Tune Monoclonal Antibodies

(2013) Xiaojie Yu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Glycosaminoglycan Glycomics Using Mass Spectrometry

(2013) Joseph Zaia   MOLECULAR & CELLULAR PROTEOMICS

Chinese hamster genome sequenced from sorted chromosomes

(2013) Karina Brinkrolf et al.   NATURE BIOTECHNOLOGY

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

(2013) Nathan E Lewis et al.   NATURE BIOTECHNOLOGY

Recombinant human lecithin-cholesterol acyltransferase Fc fusion: Analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region

(2013) Chris Spahr et al.   PROTEIN SCIENCE

Glycosaminoglycan polysaccharide biosynthesis and production: today and tomorrow

(2012) Paul L. DeAngelis   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recent advances in technology supporting biopharmaceutical production from mammalian cells

(2012) M. Butler et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Sialic acid metabolism and sialyltransferases: natural functions and applications

(2012) Yanhong Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation

(2012) Darius Ghaderi et al.   Biotechnology & Genetic Engineering Reviews

Identifying the CHO Secretome using Mucin-type O-Linked Glycosylation and Click-chemistry

(2012) Peter G. Slade et al.   JOURNAL OF PROTEOME RESEARCH

Proteomic Analysis of Chinese Hamster Ovary Cells

(2012) Deniz Baycin-Hizal et al.   JOURNAL OF PROTEOME RESEARCH

Heparosan-Derived Heparan Sulfate/Heparin-Like Compounds: One Kind of Potential Therapeutic Agents

(2012) Pingli Li et al.   MEDICINAL RESEARCH REVIEWS

Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin

(2012) Jong Youn Baik et al.   METABOLIC ENGINEERING

Effect of heparin on the outcome of IVF treatment: a systematic review and meta-analysis

(2012) S. Seshadri et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Engineering of routes to heparin and related polysaccharides

(2011) Ujjwal Bhaskar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancement of recombinant human EPO production and sialylation in chinese hamster ovary cells through Bombyx mori 30Kc19 gene expression

(2011) Zesong Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mammalian cells as biopharmaceutical production hosts in the age of omics

(2011) Stefanie Dietmair et al.   Biotechnology Journal

A dynamic mathematical model for monoclonal antibody N-linked glycosylation and nucleotide sugar donor transport within a maturing Golgi apparatus

(2011) Ioscani Jimenez del Val et al.   BIOTECHNOLOGY PROGRESS

Enhanced sialylation of recombinant human erythropoietin in Chinese hamster ovary cells by combinatorial engineering of selected genes

(2011) Y.-D. Son et al.   GLYCOBIOLOGY

High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans

(2011) A. M. Goetze et al.   GLYCOBIOLOGY

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing

(2011) Jennifer Becker et al.   JOURNAL OF BIOTECHNOLOGY

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies

(2010) Laetitia Malphettes et al.   BIOTECHNOLOGY AND BIOENGINEERING

An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding

(2010) Niki S.C. Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Malignant melanoma as a target malignancy for the study of the anti-metastatic properties of the heparins

(2010) Anthony Maraveyas et al.   CANCER AND METASTASIS REVIEWS

Superior In vivo Efficacy of Afucosylated Trastuzumab in the Treatment of HER2-Amplified Breast Cancer

(2010) Teemu T. Junttila et al.   CANCER RESEARCH

Antitumor Effect of Butanoylated Heparin with Low Anticoagulant Activity on Lung Cancer Growth in Mice and Rats

(2010) Lunyin Yu et al.   CURRENT CANCER DRUG TARGETS

Post-translational modifications of protein biopharmaceuticals

(2010) Gary Walsh   DRUG DISCOVERY TODAY

Hypoxic regulation of glycosylation via the N-acetylglucosamine cycle

(2010) Ken Shirato et al.   JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION

The potential benefits of low-molecular-weight heparins in cancer patients

(2010) Francisco Robert   Journal of Hematology & Oncology

Industrialization of mAb production technology: The bioprocessing industry at a crossroads

(2010) Brian Kelley   mAbs

Heparin-derived heparan sulfate mimics to modulate heparan sulfate-protein interaction in inflammation and cancer

(2010) Benito Casu et al.   MATRIX BIOLOGY

Chinese hamster ovary cells can produce galactose-α-1,3-galactose antigens on proteins

(2010) Carlos J Bosques et al.   NATURE BIOTECHNOLOGY

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins

(2010) Darius Ghaderi et al.   NATURE BIOTECHNOLOGY

A mathematical model to derive N-glycan structures and cellular enzyme activities from mass spectrometric data

(2009) Frederick J Krambeck et al.   GLYCOBIOLOGY

Glycomics Profiling of Chinese Hamster Ovary Cell Glycosylation Mutants RevealsN-Glycans of a Novel Size and Complexity

(2009) Simon J. North et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lessons learned from the contamination of heparin

(2009) Haiying Liu et al.   NATURAL PRODUCT REPORTS

Enhanced sialylation of recombinant erythropoietin in genetically engineered Chinese-hamster ovary cells

(2008) Yeon Tae Jeong et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon-γ glycosylation during batch and fed-batch cultures of CHO cells

(2008) N. Kochanowski et al.   BIOTECHNOLOGY AND BIOENGINEERING

Branched N-glycans regulate the biological functions of integrins and cadherins

(2008) Yanyang Zhao et al.   FEBS Journal

Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease

(2008) V. Padler-Karavani et al.   GLYCOBIOLOGY

Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression

(2008) M. Hedlund et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recapitulation of IVIG Anti-Inflammatory Activity with a Recombinant IgG Fc

(2008) R. M. Anthony et al.   SCIENCE

The Galα1,3Galβ1,4GlcNAc-R (α-Gal) epitope: A carbohydrate of unique evolution and clinical relevance

(2007) Bruce A. Macher et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Novel long-lasting interferon alpha derivatives designed by glycoengineering

(2007) N. Ceaglio et al.   BIOCHIMIE