Single Pair Förster Resonance Energy Transfer: A Versatile Tool To Investigate Protein Conformational Dynamics

48-spot single-molecule FRET setup with periodic acceptor excitation

(2018) Antonino Ingargiola et al.   JOURNAL OF CHEMICAL PHYSICS

Combining Graphical and Analytical Methods with Molecular Simulations To Analyze Time-Resolved FRET Measurements of Labeled Macromolecules Accurately

(2017) Thomas-Otavio Peulen et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Conformational dynamics of dynamin-like MxA revealed by single-molecule FRET

(2017) Yang Chen et al.   Nature Communications

Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules

(2017) Antonino Ingargiola et al.   PLoS One

Nanoscale devices for linkerless long-term single-molecule observation

(2016) Niccolò Banterle et al.   CURRENT OPINION IN BIOTECHNOLOGY

Multidomain structure and correlated dynamics determined by self-consistent FRET networks

(2016) Björn Hellenkamp et al.   NATURE METHODS

Single-molecule-sensitive fluorescence resonance energy transfer in freely-diffusing attoliter droplets

(2015) Sheema Rahmanseresht et al.   APPLIED PHYSICS LETTERS

Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond

(2015) Ivana Nikić et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Quantitative single molecule FRET efficiencies using TIRF microscopy

(2015) Lasse L. Hildebrandt et al.   FARADAY DISCUSSIONS

Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α

(2015) Elise Delaforge et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Conformational dynamics of a class C G-protein-coupled receptor

(2015) Reza Vafabakhsh et al.   NATURE

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

(2015) Iwo König et al.   NATURE METHODS

Direct observation of TALE protein dynamics reveals a two-state search mechanism

(2015) Luke Cuculis et al.   Nature Communications

Quantitative single molecule FRET efficiencies using TIRF microscopy

(2015) Lasse L. Hildebrandt et al.   FARADAY DISCUSSIONS

Geminate Recombination as a Photoprotection Mechanism for Fluorescent Dyes

(2014) Phil Holzmeister et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Exploding Genetic Code

(2014) Edward A. Lemke   CHEMBIOCHEM

Probing Protein Multidimensional Conformational Fluctuations by Single-Molecule Multiparameter Photon Stamping Spectroscopy

(2014) Maolin Lu et al.   JOURNAL OF PHYSICAL CHEMISTRY B

An improved surface passivation method for single-molecule studies

(2014) Boyang Hua et al.   NATURE METHODS

Continuous throughput and long-term observation of single-molecule FRET without immobilization

(2014) Swati Tyagi et al.   NATURE METHODS

Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ

(2014) Giorgos Gouridis et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules

(2014) A. Plochowietz et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Fine tuning of sub-millisecond conformational dynamics controls metabotropic glutamate receptors agonist efficacy

(2014) Linnea Olofsson et al.   Nature Communications

Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET

(2013) Martin Sikor et al.   EMBO JOURNAL

High performance, LED powered, waveguide based total internal reflection microscopy

(2013) Srinivasan Ramachandran et al.   Scientific Reports

Filtered FCS: Species Auto- and Cross-Correlation Functions Highlight Binding and Dynamics in Biomolecules

(2012) Suren Felekyan et al.   CHEMPHYSCHEM

Combining MFD and PIE for Accurate Single-Pair Förster Resonance Energy Transfer Measurements

(2012) Volodymyr Kudryavtsev et al.   CHEMPHYSCHEM

Dynamic Architecture of a Minimal RNA Polymerase II Open Promoter Complex

(2012) Barbara Treutlein et al.   MOLECULAR CELL

'Self-healing' dyes: intramolecular stabilization of organic fluorophores

(2012) Philip Tinnefeld et al.   NATURE METHODS

Enhanced photostability of cyanine fluorophores across the visible spectrum

(2012) Roger B Altman et al.   NATURE METHODS

A toolkit and benchmark study for FRET-restrained high-precision structural modeling

(2012) Stanislav Kalinin et al.   NATURE METHODS

Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules

(2012) Saumya Saurabh et al.   OPTICS EXPRESS

Single-molecule photon stamping FRET spectroscopy study of enzymatic conformational dynamics

(2012) Yufan He et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Quantifying internal friction in unfolded and intrinsically disordered proteins with single-molecule spectroscopy

(2012) A. Soranno et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Theory of the energy transfer efficiency and fluorescence lifetime distribution in single-molecule FRET

(2012) I. V. Gopich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-Molecule Fluorescence Experiments Determine Protein Folding Transition Path Times

(2012) H. S. Chung et al.   SCIENCE

Fluorescence Correlation Spectroscopy: A Review of Biochemical and Microfluidic Applications

(2011) Yu Tian et al.   APPLIED SPECTROSCOPY

Single Molecule Study of the Intrinsically Disordered FG-Repeat Nucleoporin 153

(2011) Sigrid Milles et al.   BIOPHYSICAL JOURNAL

Fluorescence Correlation Spectroscopy: Past, Present, Future

(2011) Elliot L. Elson   BIOPHYSICAL JOURNAL

FRET-FCS Detection of Intralobe Dynamics in Calmodulin

(2011) E. Shane Price et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Accurate Distance Determination of Nucleic Acids via Förster Resonance Energy Transfer: Implications of Dye Linker Length and Rigidity

(2011) Simon Sindbert et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cyanine fluorophore derivatives with enhanced photostability

(2011) Roger B Altman et al.   NATURE METHODS

Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing

(2011) Yann Gambin et al.   NATURE METHODS

Substrate discrimination of the chaperone BiP by autonomous and cochaperone-regulated conformational transitions

(2011) Moritz Marcinowski et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Quantifying heterogeneity and conformational dynamics from single molecule FRET of diffusing molecules: recurrence analysis of single particles (RASP)

(2011) Armin Hoffmann et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

Distinguishing between Protein Dynamics and Dye Photophysics in Single-Molecule FRET Experiments

(2010) Hoi Sung Chung et al.   BIOPHYSICAL JOURNAL

Characterizing Single-Molecule FRET Dynamics with Probability Distribution Analysis

(2010) Yusdi Santoso et al.   CHEMPHYSCHEM

Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon

(2010) Travis S. Young et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection of Structural Dynamics by FRET: A Photon Distribution and Fluorescence Lifetime Analysis of Systems with Multiple States

(2010) Stanislav Kalinin et al.   JOURNAL OF PHYSICAL CHEMISTRY B

The Conformational Dynamics of the Mitochondrial Hsp70 Chaperone

(2010) Koyeli Mapa et al.   MOLECULAR CELL

Single-molecule spectroscopy of protein folding in a chaperonin cage

(2010) H. Hofmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimal strategy for trapping single fluorescent molecules in solution using the ABEL trap

(2009) Q. Wang et al.   APPLIED PHYSICS B-LASERS AND OPTICS

A novel form of Total Internal Reflection Fluorescence Microscopy (LG-TIRFM) reveals different and independent lipid raft domains in living cells

(2009) Alexander Asanov et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Nonnatural Amino Acids for Site-Specific Protein Conjugation

(2009) Albert J. de Graaf et al.   BIOCONJUGATE CHEMISTRY

FRET Fluctuation Spectroscopy of Diffusing Biopolymers: Contributions of Conformational Dynamics and Translational Diffusion

(2009) Kaushik Gurunathan et al.   JOURNAL OF PHYSICAL CHEMISTRY B

On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent

(2009) Thorben Cordes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Quantitative and Qualitative Evaluation of Adsorption/Desorption of Bovine Serum Albumin on Hydrophilic and Hydrophobic Surfaces

(2009) Y. L. Jeyachandran et al.   LANGMUIR

Nucleosome disassembly intermediates characterized by single-molecule FRET

(2009) A. Gansen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interplay of  -synuclein binding and conformational switching probed by single-molecule fluorescence

(2009) A. C. M. Ferreon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct single-molecule observation of a protein living in two opposed native structures

(2009) Y. Gambin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories

(2009) H. S. Chung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Waveguide evanescent field fluorescence microscopy: Thin film fluorescence intensities and its application in cell biology

(2008) Abdollah Hassanzadeh et al.   APPLIED PHYSICS LETTERS

Monitoring Protein Conformation along the Pathway of Chaperonin-Assisted Folding

(2008) Shruti Sharma et al.   CELL

Probing Transient Copper Chaperone−Wilson Disease Protein Interactions at the Single-Molecule Level with Nanovesicle Trapping

(2008) Jaime J. Benítez et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The importance of surfaces in single-molecule bioscience

(2008) Mari-Liis Visnapuu et al.   Molecular BioSystems

A nano-positioning system for macromolecular structural analysis

(2008) Adam Muschielok et al.   NATURE METHODS

A practical guide to single-molecule FRET

(2008) Rahul Roy et al.   NATURE METHODS

An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments

(2007) Colin Echeverría Aitken et al.   BIOPHYSICAL JOURNAL